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The map label for this gene is nuoB

Identifier: 50843385

GI number: 50843385

Start: 2096758

End: 2097315

Strand: Reverse

Name: nuoB

Synonym: PPA1935

Alternate gene names: 50843385

Gene position: 2097315-2096758 (Counterclockwise)

Preceding gene: 50843386

Following gene: 50843384

Centisome position: 81.92

GC content: 62.37

Gene sequence:

>558_bases
ATGGCTGAGTACGACGCCGACGTCCCAGCTGGCATCGTGATGACCTCGATGGAGGCCATCGTCGGATGGCTCCGCAAGAC
CTCCTTCTGGCCGCTGACGATGGGGCTGGCGTGCTGCGCCATCGAGATGATTTCCTACGGTGGCCCGCGTGCCGACGCTG
CGCGCTGGGGCCACGAGGTGTTCCGTGCCTCTCCCCGTCAGGCCGACCTCATGATCGTTTCGGGCCGGGTAAGCCAGAAG
ATGGCCCCGGTGGTCCGTCAGCTTTACGACCAAATGCCCGAACCCAAGTGGGTTATCTCGATGGGGGCTTGCGCATCCTC
TGGCGGCATCTTTAATAACTATGCGGTCGTCCAGGGATGCGACCATATCGTCCCGGTGGATGTCTACCTGCCAGGCTGCC
CGCCCAGCCCGGACATGCTCATTGACGCCGTCTTCAAGATCCGTGAGCAGATCCAGCATTGGCCACTCATGGGCCACATG
TCCGAGGTCGAGGCAGCCAAGGAGAAGAAGGCCCTCGACGCCAAGACCACTCTCGAGCAGAAGGGGCTCATGCGATGA

Upstream 100 bases:

>100_bases
GACTTGATCTTCCCGCGCGGCCTGGGGGTCGCGCTCAGCAATATGTTGAGTGGCCTGCGAGCCACGTCACTCAACGTCTT
AGAAAGAAAGGATGCGCGAG

Downstream 100 bases:

>100_bases
GTGACAAGCCGACCCCGCGCGGCGCCGCAGGCGGCTCGGACTCCGGGGATCGCATCCCCGATGGCCGCAACTTCCTTGAT
CCCAGCCATCCCGACCAGGT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 185; Mature: 184

Protein sequence:

>185_residues
MAEYDADVPAGIVMTSMEAIVGWLRKTSFWPLTMGLACCAIEMISYGGPRADAARWGHEVFRASPRQADLMIVSGRVSQK
MAPVVRQLYDQMPEPKWVISMGACASSGGIFNNYAVVQGCDHIVPVDVYLPGCPPSPDMLIDAVFKIREQIQHWPLMGHM
SEVEAAKEKKALDAKTTLEQKGLMR

Sequences:

>Translated_185_residues
MAEYDADVPAGIVMTSMEAIVGWLRKTSFWPLTMGLACCAIEMISYGGPRADAARWGHEVFRASPRQADLMIVSGRVSQK
MAPVVRQLYDQMPEPKWVISMGACASSGGIFNNYAVVQGCDHIVPVDVYLPGCPPSPDMLIDAVFKIREQIQHWPLMGHM
SEVEAAKEKKALDAKTTLEQKGLMR
>Mature_184_residues
AEYDADVPAGIVMTSMEAIVGWLRKTSFWPLTMGLACCAIEMISYGGPRADAARWGHEVFRASPRQADLMIVSGRVSQKM
APVVRQLYDQMPEPKWVISMGACASSGGIFNNYAVVQGCDHIVPVDVYLPGCPPSPDMLIDAVFKIREQIQHWPLMGHMS
EVEAAKEKKALDAKTTLEQKGLMR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be a menaquinone. Couples the redox reaction to proton translo

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=142, Percent_Identity=51.4084507042254, Blast_Score=173, Evalue=9e-44,
Organism=Escherichia coli, GI1788624, Length=147, Percent_Identity=53.0612244897959, Blast_Score=174, Evalue=2e-45,
Organism=Escherichia coli, GI1789074, Length=104, Percent_Identity=46.1538461538462, Blast_Score=92, Evalue=2e-20,
Organism=Escherichia coli, GI1788834, Length=104, Percent_Identity=42.3076923076923, Blast_Score=85, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17509685, Length=145, Percent_Identity=49.6551724137931, Blast_Score=167, Evalue=2e-42,
Organism=Drosophila melanogaster, GI18859983, Length=141, Percent_Identity=52.4822695035461, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24642371, Length=141, Percent_Identity=52.4822695035461, Blast_Score=174, Evalue=4e-44,
Organism=Drosophila melanogaster, GI24651058, Length=138, Percent_Identity=53.6231884057971, Blast_Score=170, Evalue=4e-43,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_PROAC (Q6A6F9)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056612.1
- HSSP:   Q56218
- ProteinModelPortal:   Q6A6F9
- SMR:   Q6A6F9
- GeneID:   2931358
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1935
- NMPDR:   fig|267747.1.peg.1889
- HOGENOM:   HBG553221
- OMA:   EAKWVIS
- ProtClustDB:   PRK06411
- BioCyc:   PACN267747:PPA1935-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 20400; Mature: 20269

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
7.0 %Met     (Translated Protein)
9.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
6.5 %Met     (Mature Protein)
9.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEYDADVPAGIVMTSMEAIVGWLRKTSFWPLTMGLACCAIEMISYGGPRADAARWGHEV
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FRASPRQADLMIVSGRVSQKMAPVVRQLYDQMPEPKWVISMGACASSGGIFNNYAVVQGC
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHCC
DHIVPVDVYLPGCPPSPDMLIDAVFKIREQIQHWPLMGHMSEVEAAKEKKALDAKTTLEQ
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KGLMR
CCCCC
>Mature Secondary Structure 
AEYDADVPAGIVMTSMEAIVGWLRKTSFWPLTMGLACCAIEMISYGGPRADAARWGHEV
CCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
FRASPRQADLMIVSGRVSQKMAPVVRQLYDQMPEPKWVISMGACASSGGIFNNYAVVQGC
HHCCCCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCHHHHHCC
DHIVPVDVYLPGCPPSPDMLIDAVFKIREQIQHWPLMGHMSEVEAAKEKKALDAKTTLEQ
CCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHH
KGLMR
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA