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The map label for this gene is nuoM [H]

Identifier: 50843374

GI number: 50843374

Start: 2083615

End: 2085108

Strand: Reverse

Name: nuoM [H]

Synonym: PPA1923

Alternate gene names: 50843374

Gene position: 2085108-2083615 (Counterclockwise)

Preceding gene: 50843375

Following gene: 50843373

Centisome position: 81.44

GC content: 60.71

Gene sequence:

>1494_bases
ATGTCTTTCCCGATCCTCACAGTGATCGCGCTGCTGCCGTTCGTCGGCGGCGTTATCACCATGATGCTGCGCGGGGCTGC
GGCCCGAGCCACCGGCATGGTGTTTTCTCTGGTCACTCTGGTACTGGGCGTCTACGCTTTCATCGTCGGCGTCAATGGCA
CCAACCTTTCGGAAACCCATCGCTGGATCCGCGCTGTCGGAGCCTGGTATGCGCTAGGTGGTGATGGCCTTTCCCTGACG
ATGATCCTGCTGGTCGTCATCCTCGTGCCGGTGGTGCTACTCGCCGAGTGGCATGTGGGCGACGACGAGACGGCTCGCTG
GTCAACTGGGGCGTTTTTCGGCTTGGCCCTCATGCTTGAGGGACTGGCAATCTATTGCTTCGCTGCCACCGACGTCTTGC
TGTTCTACCTCATGTTCGAGGCCACCCTCATCCCGATGTACTTCCTCATTGCAGGATGGGGCGGTCCACGACGAGGCCGA
GCAGCGATGAAGTTCCTGCTGTACTCCTTGGCTGGTGGGCTGGTCATGCTATTCGCTGTCATCGGCGTCGGGGTGCACGG
CGGATCCTTCCTTCTGAGCGACCTGGCAAAGGTGGACTTCTCGGGGGCGATTGGTAAGGAATTATTTGTCGGATTCTTCA
TCGCCTTCGCGATCAAGGCCCCGATGGTCCCGGTGCACACGTGGCTGCCTGATACCGCCGAACAGGCCACTGCGGGGGCG
TCTTCCCTGCTCGTCGGCTTGCTCGACAAGATCGGCACCTTTGGAATGCTGAGGCTGTGTCTGGGCCTGTTCCCCGCCGC
CTCCGACTGGGCCACCCCCTTCGTCCTCACCCTGGCGATTATCTCGGTGCTGTGGGGTGCCATGATGGCCGCGACCTCGA
GCGACCTCATGAGGTTCGTCTCCTACACCTCAGTAAGTCACTTCGGCTTTATGGTCATTGGCATTTTCGCGCTGACGACG
ACGTCGATCACAGGTTCGATCTTCTACATGTTCAACCACGGCTTTTCGACGGCAGCGTTGTTCCTCGTGCTTGGTTTCCT
GGTGCGCAGGGGCGGGACGGCGAAGGTCGACGGTTACGGTGGTGTCCAGAAGGTCGCCCCGGTACTTGCCGGAACTTTCC
TCTTCGCCGGGCTGGCAACCCTGTCGTTGCCGGGCACGGGAAACTTTGTGAGCGAGTTCATGGCCTTAGCTGGTGCTTGG
TCGCGTCACCCAATCTATGTCGCCATTTCCACCATCGGCATGGTGCTGGCTGCGGTGTACGTTCTGGTGACGTACCGACG
CACTATGACCGGCCCGGTGCAGGGTGTCTCTGAGCAGGTCAGTGACCTGAACGGGCGGGAGAAGTTGGTGCTTGCACCTC
TCATCATTTTGCTGCTGGTGTTCGGGTTCTTCCCCAGGCCGATGCTGCACGCCATCGAACCAGCGGCCACGGCCACCATG
ACTCAGGTCCACGCCACCGATCCGGCACCCGTCGTCAAGGAGGGTCTCAAGTGA

Upstream 100 bases:

>100_bases
GCTACGTGCGCAGTTACGGCCTCATGATGGTCGTCGGCGTGGTGGTCGTCGGACTCGTCGTCGTCCTGGGCCGGATGGCC
TGAGCGCTGGAGGAGCTGAC

Downstream 100 bases:

>100_bases
ATCCCGCCCTGTTGATTACTGCGTCTGGGCTGCCAGCGCTCCTCGACGTCGCGGCTCCGCACCTCGAGTGGGGGCCGCTG
GCGCCGGTGTTCGTCCTCAT

Product: NADH dehydrogenase subunit M

Products: NA

Alternate protein names: NADH dehydrogenase I subunit M; NDH-1 subunit M [H]

Number of amino acids: Translated: 497; Mature: 496

Protein sequence:

>497_residues
MSFPILTVIALLPFVGGVITMMLRGAAARATGMVFSLVTLVLGVYAFIVGVNGTNLSETHRWIRAVGAWYALGGDGLSLT
MILLVVILVPVVLLAEWHVGDDETARWSTGAFFGLALMLEGLAIYCFAATDVLLFYLMFEATLIPMYFLIAGWGGPRRGR
AAMKFLLYSLAGGLVMLFAVIGVGVHGGSFLLSDLAKVDFSGAIGKELFVGFFIAFAIKAPMVPVHTWLPDTAEQATAGA
SSLLVGLLDKIGTFGMLRLCLGLFPAASDWATPFVLTLAIISVLWGAMMAATSSDLMRFVSYTSVSHFGFMVIGIFALTT
TSITGSIFYMFNHGFSTAALFLVLGFLVRRGGTAKVDGYGGVQKVAPVLAGTFLFAGLATLSLPGTGNFVSEFMALAGAW
SRHPIYVAISTIGMVLAAVYVLVTYRRTMTGPVQGVSEQVSDLNGREKLVLAPLIILLLVFGFFPRPMLHAIEPAATATM
TQVHATDPAPVVKEGLK

Sequences:

>Translated_497_residues
MSFPILTVIALLPFVGGVITMMLRGAAARATGMVFSLVTLVLGVYAFIVGVNGTNLSETHRWIRAVGAWYALGGDGLSLT
MILLVVILVPVVLLAEWHVGDDETARWSTGAFFGLALMLEGLAIYCFAATDVLLFYLMFEATLIPMYFLIAGWGGPRRGR
AAMKFLLYSLAGGLVMLFAVIGVGVHGGSFLLSDLAKVDFSGAIGKELFVGFFIAFAIKAPMVPVHTWLPDTAEQATAGA
SSLLVGLLDKIGTFGMLRLCLGLFPAASDWATPFVLTLAIISVLWGAMMAATSSDLMRFVSYTSVSHFGFMVIGIFALTT
TSITGSIFYMFNHGFSTAALFLVLGFLVRRGGTAKVDGYGGVQKVAPVLAGTFLFAGLATLSLPGTGNFVSEFMALAGAW
SRHPIYVAISTIGMVLAAVYVLVTYRRTMTGPVQGVSEQVSDLNGREKLVLAPLIILLLVFGFFPRPMLHAIEPAATATM
TQVHATDPAPVVKEGLK
>Mature_496_residues
SFPILTVIALLPFVGGVITMMLRGAAARATGMVFSLVTLVLGVYAFIVGVNGTNLSETHRWIRAVGAWYALGGDGLSLTM
ILLVVILVPVVLLAEWHVGDDETARWSTGAFFGLALMLEGLAIYCFAATDVLLFYLMFEATLIPMYFLIAGWGGPRRGRA
AMKFLLYSLAGGLVMLFAVIGVGVHGGSFLLSDLAKVDFSGAIGKELFVGFFIAFAIKAPMVPVHTWLPDTAEQATAGAS
SLLVGLLDKIGTFGMLRLCLGLFPAASDWATPFVLTLAIISVLWGAMMAATSSDLMRFVSYTSVSHFGFMVIGIFALTTT
SITGSIFYMFNHGFSTAALFLVLGFLVRRGGTAKVDGYGGVQKVAPVLAGTFLFAGLATLSLPGTGNFVSEFMALAGAWS
RHPIYVAISTIGMVLAAVYVLVTYRRTMTGPVQGVSEQVSDLNGREKLVLAPLIILLLVFGFFPRPMLHAIEPAATATMT
QVHATDPAPVVKEGLK

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be menaquinone. Couples the redox reaction to proton transloca

COG id: COG1008

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 4 (chain M)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 4 family [H]

Homologues:

Organism=Homo sapiens, GI251831116, Length=378, Percent_Identity=29.3650793650794, Blast_Score=145, Evalue=7e-35,
Organism=Escherichia coli, GI1788613, Length=499, Percent_Identity=36.0721442885772, Blast_Score=245, Evalue=5e-66,
Organism=Escherichia coli, GI1788827, Length=272, Percent_Identity=26.1029411764706, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1788831, Length=370, Percent_Identity=22.7027027027027, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788829, Length=471, Percent_Identity=25.2653927813163, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI145693160, Length=355, Percent_Identity=25.0704225352113, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI1788614, Length=511, Percent_Identity=25.6360078277886, Blast_Score=72, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010227
- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 52803; Mature: 52671

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHEECCCCCHHHHCCCC
>Mature Secondary Structure 
SFPILTVIALLPFVGGVITMMLRGAAARATGMVFSLVTLVLGVYAFIVGVNGTNLSETH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHH
RWIRAVGAWYALGGDGLSLTMILLVVILVPVVLLAEWHVGDDETARWSTGAFFGLALMLE
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
GLAIYCFAATDVLLFYLMFEATLIPMYFLIAGWGGPRRGRAAMKFLLYSLAGGLVMLFAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGVGVHGGSFLLSDLAKVDFSGAIGKELFVGFFIAFAIKAPMVPVHTWLPDTAEQATAGA
HHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCHHHHHCCH
SSLLVGLLDKIGTFGMLRLCLGLFPAASDWATPFVLTLAIISVLWGAMMAATSSDLMRFV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYTSVSHFGFMVIGIFALTTTSITGSIFYMFNHGFSTAALFLVLGFLVRRGGTAKVDGYG
HHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHCCCCCEECCCC
GVQKVAPVLAGTFLFAGLATLSLPGTGNFVSEFMALAGAWSRHPIYVAISTIGMVLAAVY
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
VLVTYRRTMTGPVQGVSEQVSDLNGREKLVLAPLIILLLVFGFFPRPMLHAIEPAATATM
HHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHH
TQVHATDPAPVVKEGLK
HHEECCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]