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The map label for this gene is yngF [H]

Identifier: 50843351

GI number: 50843351

Start: 2059087

End: 2060013

Strand: Reverse

Name: yngF [H]

Synonym: PPA1899

Alternate gene names: 50843351

Gene position: 2060013-2059087 (Counterclockwise)

Preceding gene: 50843352

Following gene: 50843349

Centisome position: 80.46

GC content: 62.35

Gene sequence:

>927_bases
GTGAAGATCAGCGGTACCGACGATGTCACGATCATCTTGGACCGTCCCCGAGCGATCAACTCGTTATCTGGTGAGATGCT
GGGGGCTATTGGGGAGGCCGTCAAGGGATCGCCGCGCAGCATCGATCTATCCGGTGCGGGAGAGCGCGGCTACTGTTCGG
GAGCTGACATTCGCGAGCTCAGATCGTTGGTATTAAAGGATCCCGAGGCCGCTGGTGACTGGTTGGATGGTGAGTACGAC
GTTGACGCTGCCATTGCTGAGGTGCATTCGGGGACCGCGCATCTGCATGGCATTTCCATGGGCGGCGGGCTTGGGTTGGC
ACTGCGCCTTAAGCGGGTCGAGGCTCGCGACGACCTCGTCCTTGCGATGCCGGAAACGGGTATTGGTCTATGGCCTGACG
TGGGTGCGTGTTTCGAGCTCTCTCGGGCCCCGCGCCTTGTCGGCCGCCATATAGCCATGACCGGGGCCTTCATCGATGCG
GCTTCGGCGCTGTGGGCCGGTTTGGTAGATGAGGTCGTCGACGCCGACGGCGAGCCCGTTGACGTCGATCCGGTCGCTTG
TCAGTTGGCGCGCGATGCGGTGTGGATTCAGGAGTGTTATGACACTGATGATCCCGTCGAGGTGTGTCGTCGATTGGCTG
ATCGTCCCGAGCCGGCCGCGCGTAAGACGGCTGAGCACATCGCGACGCGCTGTCCGTTGTCGGTAGCTGTCGCATTGGCC
GCTGTCGTGAGGGCTGAATCCGCGTCAGGTCTTGGCGAGGTGTTGGAGACTGATCGTGCTCTGGGGCGGTCCTTTATGCG
CAATTCGGACTTTTGTGAGGGGGTGCGCGCCCAGCTGGTCGACAAGGACCGCAACCCGCATTGGTCCCACGAGAGCGTGG
CTGACGTGCCCGCTCGTCAGGTTGAGGGGATGTTCGACCCGTCCTGA

Upstream 100 bases:

>100_bases
CCGGTATGCAGCGGATAGTTCGATGGCGGTAGCCGTGGTTGTTGGGAGCGGAAAGTGACGACGCGAGTTCTTGACGTGGC
AGGCTGGGGATATGGATGAG

Downstream 100 bases:

>100_bases
CTTACAGGTCATAGTCGACGACTACTGCTGCGTGGTCGGAGGTGCGCTCGGAGTAGGAGGGGTCACGATCAATCTCGGCC
GTGATGGCTTTGCTCGCCAA

Product: enoyl-CoA hydratase/isomerase family protein

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MKISGTDDVTIILDRPRAINSLSGEMLGAIGEAVKGSPRSIDLSGAGERGYCSGADIRELRSLVLKDPEAAGDWLDGEYD
VDAAIAEVHSGTAHLHGISMGGGLGLALRLKRVEARDDLVLAMPETGIGLWPDVGACFELSRAPRLVGRHIAMTGAFIDA
ASALWAGLVDEVVDADGEPVDVDPVACQLARDAVWIQECYDTDDPVEVCRRLADRPEPAARKTAEHIATRCPLSVAVALA
AVVRAESASGLGEVLETDRALGRSFMRNSDFCEGVRAQLVDKDRNPHWSHESVADVPARQVEGMFDPS

Sequences:

>Translated_308_residues
MKISGTDDVTIILDRPRAINSLSGEMLGAIGEAVKGSPRSIDLSGAGERGYCSGADIRELRSLVLKDPEAAGDWLDGEYD
VDAAIAEVHSGTAHLHGISMGGGLGLALRLKRVEARDDLVLAMPETGIGLWPDVGACFELSRAPRLVGRHIAMTGAFIDA
ASALWAGLVDEVVDADGEPVDVDPVACQLARDAVWIQECYDTDDPVEVCRRLADRPEPAARKTAEHIATRCPLSVAVALA
AVVRAESASGLGEVLETDRALGRSFMRNSDFCEGVRAQLVDKDRNPHWSHESVADVPARQVEGMFDPS
>Mature_308_residues
MKISGTDDVTIILDRPRAINSLSGEMLGAIGEAVKGSPRSIDLSGAGERGYCSGADIRELRSLVLKDPEAAGDWLDGEYD
VDAAIAEVHSGTAHLHGISMGGGLGLALRLKRVEARDDLVLAMPETGIGLWPDVGACFELSRAPRLVGRHIAMTGAFIDA
ASALWAGLVDEVVDADGEPVDVDPVACQLARDAVWIQECYDTDDPVEVCRRLADRPEPAARKTAEHIATRCPLSVAVALA
AVVRAESASGLGEVLETDRALGRSFMRNSDFCEGVRAQLVDKDRNPHWSHESVADVPARQVEGMFDPS

Specific function: FADb And FADa Are The Alpha And Beta Subunits Of The Multifunctional Enzyme Complex Of The Fatty Acid Degradation Cycle. [C]

COG id: COG1024

COG function: function code I; Enoyl-CoA hydratase/carnithine racemase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI37594471, Length=329, Percent_Identity=27.6595744680851, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI37594469, Length=290, Percent_Identity=25.8620689655172, Blast_Score=95, Evalue=9e-20,
Organism=Escherichia coli, GI1790281, Length=175, Percent_Identity=30.8571428571429, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI25144160, Length=334, Percent_Identity=28.7425149700599, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI25144157, Length=334, Percent_Identity=28.7425149700599, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320241, Length=344, Percent_Identity=23.8372093023256, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI28571730, Length=322, Percent_Identity=29.8136645962733, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571729, Length=322, Percent_Identity=29.8136645962733, Blast_Score=133, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 32802; Mature: 32802

Theoretical pI: Translated: 4.43; Mature: 4.43

Prosite motif: PS00024 HEMOPEXIN ; PS00879 ODR_DC_2_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISGTDDVTIILDRPRAINSLSGEMLGAIGEAVKGSPRSIDLSGAGERGYCSGADIREL
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHH
RSLVLKDPEAAGDWLDGEYDVDAAIAEVHSGTAHLHGISMGGGLGLALRLKRVEARDDLV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCEE
LAMPETGIGLWPDVGACFELSRAPRLVGRHIAMTGAFIDAASALWAGLVDEVVDADGEPV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DVDPVACQLARDAVWIQECYDTDDPVEVCRRLADRPEPAARKTAEHIATRCPLSVAVALA
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
AVVRAESASGLGEVLETDRALGRSFMRNSDFCEGVRAQLVDKDRNPHWSHESVADVPARQ
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHH
VEGMFDPS
HCCCCCCC
>Mature Secondary Structure
MKISGTDDVTIILDRPRAINSLSGEMLGAIGEAVKGSPRSIDLSGAGERGYCSGADIREL
CCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHH
RSLVLKDPEAAGDWLDGEYDVDAAIAEVHSGTAHLHGISMGGGLGLALRLKRVEARDDLV
HHHHHCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHCCCCCEE
LAMPETGIGLWPDVGACFELSRAPRLVGRHIAMTGAFIDAASALWAGLVDEVVDADGEPV
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
DVDPVACQLARDAVWIQECYDTDDPVEVCRRLADRPEPAARKTAEHIATRCPLSVAVALA
CCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHH
AVVRAESASGLGEVLETDRALGRSFMRNSDFCEGVRAQLVDKDRNPHWSHESVADVPARQ
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHH
VEGMFDPS
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.066 {acetoacetyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]