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The map label for this gene is exoA [H]

Identifier: 50843350

GI number: 50843350

Start: 2058192

End: 2059085

Strand: Direct

Name: exoA [H]

Synonym: PPA1898

Alternate gene names: 50843350

Gene position: 2058192-2059085 (Clockwise)

Preceding gene: 50843331

Following gene: 50843356

Centisome position: 80.39

GC content: 62.98

Gene sequence:

>894_bases
ATGCGCATCGCCAGCTTCAACATCAACGGTATCCGAGCTGCCCTGAAGCGGGGATTCTCCGAGTGGATGGAGGCTCGTGA
CTGCGACGTAATCGGACTGCAAGAAGTGCGTTGCCGTCCTGCCGACCTACCCGAAGGAGCCTTCGGCGACCGTCACGTCT
CCTGGGCACCCGGCTCGCTGGCCGGACGCAACGGCGTCGCTCTCCTCACCCGTACCGCCCCGGCCGAGGTCCGGTCCTGG
GGAACCCACCTCGTCATGGCACCCGGCCAGGCTCCTACACCCGCACAGGCACTCACAGGCAACACCGACGGCCTCCCCAC
CGAATTGCTGCCCTTCGCCGACGAGGGACGTTACATCGAGGTCGACCTGGCCGATACTCCCCTCACCGTCGCTTGCCTCT
ACCTGCCCAAAGGCGCCACCCCGGATCCAGCCAACGAGAAAACCATCGCCAAGCAGGACCGCAAAATGGCCTTCCTGACA
GGGTTCCGCGACCACCTAGTCCGACGCCGCCAAGAATGCACCACCCAAGGCCGTCACTTCCTCGTTATGGGTGACTTCAA
CATCGCCCACGAAAACGCCGACCTCAAGAACTGGAAAGCCAACCAGCGCAATGAGGGATTCCTACCCGAAGAGAGGCAGT
GGTTTGACACCATCCTGTCCCCCGACACCCTCATTGACGTTGTGCGCGCCCAGCACCCCGACACCAACGGGCCCTACTCG
TGGTGGAGCTGGCGCGGCAAGGCCTTCGTTAATGACGCGGGCTGGCGTATCGACTACCACCTGGCGTCACCAGAGTTGGC
GAGCAAAGCCATCACGGCCGAGATTGATCGTGACCCCTCCTACTCCGAGCGCACCTCCGACCACGCAGCAGTAGTCGTCG
ACTATGACCTGTAA

Upstream 100 bases:

>100_bases
CAGGCCGCACTCGTACCACCCACCACCAGAGACGTCACCGGTTGCACGAGATCGCCCAGGACAACCGCCTCCGTACTCCG
CGTGTGACACACTCCTGCAT

Downstream 100 bases:

>100_bases
GTCAGGACGGGTCGAACATCCCCTCAACCTGACGAGCGGGCACGTCAGCCACGCTCTCGTGGGACCAATGCGGGTTGCGG
TCCTTGTCGACCAGCTGGGC

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MRIASFNINGIRAALKRGFSEWMEARDCDVIGLQEVRCRPADLPEGAFGDRHVSWAPGSLAGRNGVALLTRTAPAEVRSW
GTHLVMAPGQAPTPAQALTGNTDGLPTELLPFADEGRYIEVDLADTPLTVACLYLPKGATPDPANEKTIAKQDRKMAFLT
GFRDHLVRRRQECTTQGRHFLVMGDFNIAHENADLKNWKANQRNEGFLPEERQWFDTILSPDTLIDVVRAQHPDTNGPYS
WWSWRGKAFVNDAGWRIDYHLASPELASKAITAEIDRDPSYSERTSDHAAVVVDYDL

Sequences:

>Translated_297_residues
MRIASFNINGIRAALKRGFSEWMEARDCDVIGLQEVRCRPADLPEGAFGDRHVSWAPGSLAGRNGVALLTRTAPAEVRSW
GTHLVMAPGQAPTPAQALTGNTDGLPTELLPFADEGRYIEVDLADTPLTVACLYLPKGATPDPANEKTIAKQDRKMAFLT
GFRDHLVRRRQECTTQGRHFLVMGDFNIAHENADLKNWKANQRNEGFLPEERQWFDTILSPDTLIDVVRAQHPDTNGPYS
WWSWRGKAFVNDAGWRIDYHLASPELASKAITAEIDRDPSYSERTSDHAAVVVDYDL
>Mature_297_residues
MRIASFNINGIRAALKRGFSEWMEARDCDVIGLQEVRCRPADLPEGAFGDRHVSWAPGSLAGRNGVALLTRTAPAEVRSW
GTHLVMAPGQAPTPAQALTGNTDGLPTELLPFADEGRYIEVDLADTPLTVACLYLPKGATPDPANEKTIAKQDRKMAFLT
GFRDHLVRRRQECTTQGRHFLVMGDFNIAHENADLKNWKANQRNEGFLPEERQWFDTILSPDTLIDVVRAQHPDTNGPYS
WWSWRGKAFVNDAGWRIDYHLASPELASKAITAEIDRDPSYSERTSDHAAVVVDYDL

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=297, Percent_Identity=27.9461279461279, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI18375503, Length=297, Percent_Identity=27.9461279461279, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI18375501, Length=297, Percent_Identity=27.9461279461279, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1788046, Length=297, Percent_Identity=28.2828282828283, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71989536, Length=301, Percent_Identity=25.9136212624585, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI221330655, Length=301, Percent_Identity=25.2491694352159, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17136678, Length=301, Percent_Identity=25.2491694352159, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 33125; Mature: 33125

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIASFNINGIRAALKRGFSEWMEARDCDVIGLQEVRCRPADLPEGAFGDRHVSWAPGSL
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEECHHHHCCCCCCCCCCCCCCCEEEECCCCC
AGRNGVALLTRTAPAEVRSWGTHLVMAPGQAPTPAQALTGNTDGLPTELLPFADEGRYIE
CCCCCEEEEEECCCHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCEEE
VDLADTPLTVACLYLPKGATPDPANEKTIAKQDRKMAFLTGFRDHLVRRRQECTTQGRHF
EEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LVMGDFNIAHENADLKNWKANQRNEGFLPEERQWFDTILSPDTLIDVVRAQHPDTNGPYS
EEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
WWSWRGKAFVNDAGWRIDYHLASPELASKAITAEIDRDPSYSERTSDHAAVVVDYDL
EECCCCCEEEECCCCEEEEEECCCHHHHHEEEEEECCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MRIASFNINGIRAALKRGFSEWMEARDCDVIGLQEVRCRPADLPEGAFGDRHVSWAPGSL
CEEEEECCHHHHHHHHHHHHHHHHHCCCCEECHHHHCCCCCCCCCCCCCCCEEEECCCCC
AGRNGVALLTRTAPAEVRSWGTHLVMAPGQAPTPAQALTGNTDGLPTELLPFADEGRYIE
CCCCCEEEEEECCCHHHHCCCCEEEECCCCCCCCHHHHCCCCCCCCHHHCCCCCCCCEEE
VDLADTPLTVACLYLPKGATPDPANEKTIAKQDRKMAFLTGFRDHLVRRRQECTTQGRHF
EEECCCCEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LVMGDFNIAHENADLKNWKANQRNEGFLPEERQWFDTILSPDTLIDVVRAQHPDTNGPYS
EEEECCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCCCCCCC
WWSWRGKAFVNDAGWRIDYHLASPELASKAITAEIDRDPSYSERTSDHAAVVVDYDL
EECCCCCEEEECCCCEEEEEECCCHHHHHEEEEEECCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]