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The map label for this gene is dagA [H]

Identifier: 50843230

GI number: 50843230

Start: 1933262

End: 1935016

Strand: Direct

Name: dagA [H]

Synonym: PPA1770

Alternate gene names: 50843230

Gene position: 1933262-1935016 (Clockwise)

Preceding gene: 50843225

Following gene: 50843237

Centisome position: 75.51

GC content: 58.97

Gene sequence:

>1755_bases
GTGCCACTTGGCATCATGACATCTCCCGTTTCCGCGAGTCTCGAAGCCCTTGTCCCAACTCCACTGAGCTTGGATCAAGC
CATCGACAACATTGTCTCCCCCATTGCTACTGCCATTTCTGACGCGGTGTTCTGGGCTATTCCACTGCCCGGGGGAAATA
AGTTCCCGCTCATTCTGGCTTGGTTGCTTGGAGCCGGACTCTTCTTCACCATTTACCTGGGCTTCCAACAGCTGCGTCCA
GCATCGATCCGTCACTCCAGCCACGTGATGCGGGGCCACTTCTCCCGACACACCGACCCCGGTGACGTCACCAGCTTCCA
GGCCCTCGCAACCGAGTTGTCAGGAACCGTCGGCCTGGGAAACATCGCCGGTGTTGCCATCGCGATCGCAACCGGCGGCC
CGGGAGCCTCATTCTGGATTGCGGTAGCCGGATTGGTGGGCATGAGCGTCAAAATGGCTGAAGCCACCCTTGGAGTGAAA
TTCCGCGTCATCAACGAGGACGGAACCACCTCTGGTGGCCCGATGTACTATCTGCGTGACGGTCTTGCGTCGATCGGTAA
ACCCACATTGGGCCGCGTCCTTGCTTCCCTCTTCGCCATCTGCACTGCTTTCGGTGTCATTGGCGCCGGCAATATGTTCC
AGTCCAATCAAGCTGCTTATCAGCTCGCAATGTTCGCCGGCGGTCGGGATTCCTGGATCGGCACCCACATGTGGGTCGTC
GGCGTGGTCTTCGCGATCCTCGTCGGGATCGTCATCATCGGGGGCGCCTCCAAGATTGGCGCCGCAACCTCCAAAATTGT
GCCATTCATGGGCATCCTCTACGTCATCATGTGCCTGGCCGTCATTGGCGCCAATATCTCCCAGGTCGGCAGCGCCTTCG
AGGCCATCTGGTCCGGTGCCTTCACCGGCCACGGTGTCACCGGGGGGATCCTCGGAGTCCTGATCGTCGGCGTCCAGCGT
GCGGCATTCTCGAATGCGGCCGGTATCGGTACCGCTGGCATCGCCCACTCTGCGGTAAAGACGCGCCGTCCAGCCCAAGA
AGGCTTCGTTGCCATGTGGGAACCCTTCATCGACTCAGTCGTCATTTGCACTATGACGGCCATCACCATCATCATCACCG
GGGTCTATCGGAACAGTGGTGCCGATGGCGTGCAAATGACCGCCCAGGCACTAAAGACCGTTGCCCCGTGGTTCAGCTCC
CTTCTGACGCTAGCCGTAGTGCTATTCGCATTCTCGACGATGCTGTCCTACTCGTTCTATGGCAAGAAGGCCGTAGGCTA
TCTGTTCGGCAATTCGACCACCGCCGAACGTATCTACAACGCACTCTTCCTCATCTTGCTGGTAGTCGGAGCTGCGACAA
ATATGGCCTCGATTCTTGGTTTAGCCGACGCCATGCTTTTCCTCATGGCGCTGCCGAACGTGCTGGGAATGTACTTTTTA
GCGCGCGTCGTGGCACGCGAGATTAAGGGGCATCGGGAGCGTATCGACGCCGGAGTCATCCCGCAGGTTCCCGAGGATGC
CCAAGTGGGCATGATGGTGGTTAATGAGGCAACCCCCGAACAGCTCAAGAATGCCGATACCGAGTATGCTCTGCGTGCCG
CAGCCCAGGATGCCCGCAGTAAGGAGCTTAAGCTAGGACATACCGCCCCCGATACCGAGCGCGACTACCTGCAGCACCTG
CCGGAAGGCCATGAGATTTTCAACACCATGGACCGTGAAGGCAAGCCGCTCAAGAACCGACGGCACGAGTCGTAG

Upstream 100 bases:

>100_bases
GGTGTCGGTGCGCGCTGAGATCGGTCATCACAGATGGCTGGTACCACACCGTGGTGTTGCCGGGCTTGGTGTCCCATGTC
GTCAACGGATAGGACCATTC

Downstream 100 bases:

>100_bases
ATCCTGGAGAATCGGGGCCCCTCGCAATCCGCAAAGCTTCATTGCGAGGAGCCCCACTCCGCACACAGATTGGCCGGCCG
AAGGGACCGGCCGGCCAATC

Product: sodium:alanine symporter family protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 584; Mature: 583

Protein sequence:

>584_residues
MPLGIMTSPVSASLEALVPTPLSLDQAIDNIVSPIATAISDAVFWAIPLPGGNKFPLILAWLLGAGLFFTIYLGFQQLRP
ASIRHSSHVMRGHFSRHTDPGDVTSFQALATELSGTVGLGNIAGVAIAIATGGPGASFWIAVAGLVGMSVKMAEATLGVK
FRVINEDGTTSGGPMYYLRDGLASIGKPTLGRVLASLFAICTAFGVIGAGNMFQSNQAAYQLAMFAGGRDSWIGTHMWVV
GVVFAILVGIVIIGGASKIGAATSKIVPFMGILYVIMCLAVIGANISQVGSAFEAIWSGAFTGHGVTGGILGVLIVGVQR
AAFSNAAGIGTAGIAHSAVKTRRPAQEGFVAMWEPFIDSVVICTMTAITIIITGVYRNSGADGVQMTAQALKTVAPWFSS
LLTLAVVLFAFSTMLSYSFYGKKAVGYLFGNSTTAERIYNALFLILLVVGAATNMASILGLADAMLFLMALPNVLGMYFL
ARVVAREIKGHRERIDAGVIPQVPEDAQVGMMVVNEATPEQLKNADTEYALRAAAQDARSKELKLGHTAPDTERDYLQHL
PEGHEIFNTMDREGKPLKNRRHES

Sequences:

>Translated_584_residues
MPLGIMTSPVSASLEALVPTPLSLDQAIDNIVSPIATAISDAVFWAIPLPGGNKFPLILAWLLGAGLFFTIYLGFQQLRP
ASIRHSSHVMRGHFSRHTDPGDVTSFQALATELSGTVGLGNIAGVAIAIATGGPGASFWIAVAGLVGMSVKMAEATLGVK
FRVINEDGTTSGGPMYYLRDGLASIGKPTLGRVLASLFAICTAFGVIGAGNMFQSNQAAYQLAMFAGGRDSWIGTHMWVV
GVVFAILVGIVIIGGASKIGAATSKIVPFMGILYVIMCLAVIGANISQVGSAFEAIWSGAFTGHGVTGGILGVLIVGVQR
AAFSNAAGIGTAGIAHSAVKTRRPAQEGFVAMWEPFIDSVVICTMTAITIIITGVYRNSGADGVQMTAQALKTVAPWFSS
LLTLAVVLFAFSTMLSYSFYGKKAVGYLFGNSTTAERIYNALFLILLVVGAATNMASILGLADAMLFLMALPNVLGMYFL
ARVVAREIKGHRERIDAGVIPQVPEDAQVGMMVVNEATPEQLKNADTEYALRAAAQDARSKELKLGHTAPDTERDYLQHL
PEGHEIFNTMDREGKPLKNRRHES
>Mature_583_residues
PLGIMTSPVSASLEALVPTPLSLDQAIDNIVSPIATAISDAVFWAIPLPGGNKFPLILAWLLGAGLFFTIYLGFQQLRPA
SIRHSSHVMRGHFSRHTDPGDVTSFQALATELSGTVGLGNIAGVAIAIATGGPGASFWIAVAGLVGMSVKMAEATLGVKF
RVINEDGTTSGGPMYYLRDGLASIGKPTLGRVLASLFAICTAFGVIGAGNMFQSNQAAYQLAMFAGGRDSWIGTHMWVVG
VVFAILVGIVIIGGASKIGAATSKIVPFMGILYVIMCLAVIGANISQVGSAFEAIWSGAFTGHGVTGGILGVLIVGVQRA
AFSNAAGIGTAGIAHSAVKTRRPAQEGFVAMWEPFIDSVVICTMTAITIIITGVYRNSGADGVQMTAQALKTVAPWFSSL
LTLAVVLFAFSTMLSYSFYGKKAVGYLFGNSTTAERIYNALFLILLVVGAATNMASILGLADAMLFLMALPNVLGMYFLA
RVVAREIKGHRERIDAGVIPQVPEDAQVGMMVVNEATPEQLKNADTEYALRAAAQDARSKELKLGHTAPDTERDYLQHLP
EGHEIFNTMDREGKPLKNRRHES

Specific function: Catalyzes the sodium-dependent uptake of extracellular D-alanine and glycine [H]

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=451, Percent_Identity=31.7073170731707, Blast_Score=172, Evalue=6e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 61574; Mature: 61442

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLGIMTSPVSASLEALVPTPLSLDQAIDNIVSPIATAISDAVFWAIPLPGGNKFPLILA
CCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
WLLGAGLFFTIYLGFQQLRPASIRHSSHVMRGHFSRHTDPGDVTSFQALATELSGTVGLG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
NIAGVAIAIATGGPGASFWIAVAGLVGMSVKMAEATLGVKFRVINEDGTTSGGPMYYLRD
CCCCEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
GLASIGKPTLGRVLASLFAICTAFGVIGAGNMFQSNQAAYQLAMFAGGRDSWIGTHMWVV
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
GVVFAILVGIVIIGGASKIGAATSKIVPFMGILYVIMCLAVIGANISQVGSAFEAIWSGA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
FTGHGVTGGILGVLIVGVQRAAFSNAAGIGTAGIAHSAVKTRRPAQEGFVAMWEPFIDSV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHCHHHHHHHHHHHH
VICTMTAITIIITGVYRNSGADGVQMTAQALKTVAPWFSSLLTLAVVLFAFSTMLSYSFY
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKKAVGYLFGNSTTAERIYNALFLILLVVGAATNMASILGLADAMLFLMALPNVLGMYFL
CCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVVAREIKGHRERIDAGVIPQVPEDAQVGMMVVNEATPEQLKNADTEYALRAAAQDARS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHC
KELKLGHTAPDTERDYLQHLPEGHEIFNTMDREGKPLKNRRHES
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
PLGIMTSPVSASLEALVPTPLSLDQAIDNIVSPIATAISDAVFWAIPLPGGNKFPLILA
CCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHH
WLLGAGLFFTIYLGFQQLRPASIRHSSHVMRGHFSRHTDPGDVTSFQALATELSGTVGLG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCCCC
NIAGVAIAIATGGPGASFWIAVAGLVGMSVKMAEATLGVKFRVINEDGTTSGGPMYYLRD
CCCCEEEEEECCCCCHHHHHHHHHHHHCHHHHHHHHCCEEEEEECCCCCCCCCCCHHHHH
GLASIGKPTLGRVLASLFAICTAFGVIGAGNMFQSNQAAYQLAMFAGGRDSWIGTHMWVV
HHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHH
GVVFAILVGIVIIGGASKIGAATSKIVPFMGILYVIMCLAVIGANISQVGSAFEAIWSGA
HHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCC
FTGHGVTGGILGVLIVGVQRAAFSNAAGIGTAGIAHSAVKTRRPAQEGFVAMWEPFIDSV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHCHHHHHHHHHHHH
VICTMTAITIIITGVYRNSGADGVQMTAQALKTVAPWFSSLLTLAVVLFAFSTMLSYSFY
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GKKAVGYLFGNSTTAERIYNALFLILLVVGAATNMASILGLADAMLFLMALPNVLGMYFL
CCHHEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARVVAREIKGHRERIDAGVIPQVPEDAQVGMMVVNEATPEQLKNADTEYALRAAAQDARS
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCHHHHHCCCHHHHHHHHHHHHHC
KELKLGHTAPDTERDYLQHLPEGHEIFNTMDREGKPLKNRRHES
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1447975 [H]