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The map label for this gene is punA [H]

Identifier: 50843182

GI number: 50843182

Start: 1875582

End: 1876394

Strand: Direct

Name: punA [H]

Synonym: PPA1722

Alternate gene names: 50843182

Gene position: 1875582-1876394 (Clockwise)

Preceding gene: 50843180

Following gene: 50843183

Centisome position: 73.26

GC content: 63.22

Gene sequence:

>813_bases
ATGACGTCACCGGAGTTCACCGACCCCCAGGCCATCGCCCGCAATGCTGCCGATGCCATCCGTTCCCGCCTATCTCTGAC
CAGCGTCGACATGGGCCTCGTCCTTGGTTCAGGATGGTCTGCTGGAGTTGATCGCCTGGGCAAGACTCTTGGGAGGATCC
CGCTGAGTGAACTGCCCGGATTCTTTGCACCTGTTGTTGCCGGACACGACGGCGACCTCGTAGCCGTGGCCCTGCCCAGC
GGAAAGACCGCCGTTGTCCTCACTGGCCGCACTCATTTTTATGAAGGCCGCGGGGTCGAGTCGGTGGTCCACGGAGTGCG
CACTGTCGCCGCGCTAGGTGCCACCACGATGATCCTCACGAACGGTTGCGGCGGATTAGACCCGTCGTGGGGCCCGGGAA
CCATCGTCGCTATTCGTGACCACATCAACCTCACCGGTGCCACCCCACTGCGCGGCGCCACTTTCATTGATATGTCGACG
GCGTGGACTCCACACCTGCTGGAGGTCACCGAGAGGGTTGCTCCTGGCACTCCAACCGGTGTTTACGCTCAGTTCCGCGG
CCCCCAGTACGAGACCCCAGCTGAGGTTCGCATGGCTCGCACCATCGGCGCCGACCTTGTCGGAATGTCAACCGCCCTTG
AGGCCATTGCCGCCCGCGAAGCCGGTCTCGACCTACTCGGGCTGTCCCTGGTCACCAACCATGCAGCCGGGGTCACTGAT
GCCCCACTTGACCATGCCGAAGTTATCGCTGCTGGCAAGCAGGCCGCCCCTCGACTAGCTAGCCTTCTCGCCGATATCAC
GGCTGCCATCTGA

Upstream 100 bases:

>100_bases
CGACACTGAACACCCCAAACGGCTATCCCAGCTGACGAAAATCGCAGCACATGGCCGGGGCTGGCACTCCCAAACAGCGA
AAACCTCTAGGGTTAACCCC

Downstream 100 bases:

>100_bases
TCCATCCCACAGCCCATCGAGGAACCATGACCACCACCCGCCCTGAAAGGCTTAGCCCCGAACTCGTCGCAGAAGTTCAG
TCCTGGCTCGACCAAGACCC

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: PNP; Inosine phosphorylase [H]

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MTSPEFTDPQAIARNAADAIRSRLSLTSVDMGLVLGSGWSAGVDRLGKTLGRIPLSELPGFFAPVVAGHDGDLVAVALPS
GKTAVVLTGRTHFYEGRGVESVVHGVRTVAALGATTMILTNGCGGLDPSWGPGTIVAIRDHINLTGATPLRGATFIDMST
AWTPHLLEVTERVAPGTPTGVYAQFRGPQYETPAEVRMARTIGADLVGMSTALEAIAAREAGLDLLGLSLVTNHAAGVTD
APLDHAEVIAAGKQAAPRLASLLADITAAI

Sequences:

>Translated_270_residues
MTSPEFTDPQAIARNAADAIRSRLSLTSVDMGLVLGSGWSAGVDRLGKTLGRIPLSELPGFFAPVVAGHDGDLVAVALPS
GKTAVVLTGRTHFYEGRGVESVVHGVRTVAALGATTMILTNGCGGLDPSWGPGTIVAIRDHINLTGATPLRGATFIDMST
AWTPHLLEVTERVAPGTPTGVYAQFRGPQYETPAEVRMARTIGADLVGMSTALEAIAAREAGLDLLGLSLVTNHAAGVTD
APLDHAEVIAAGKQAAPRLASLLADITAAI
>Mature_269_residues
TSPEFTDPQAIARNAADAIRSRLSLTSVDMGLVLGSGWSAGVDRLGKTLGRIPLSELPGFFAPVVAGHDGDLVAVALPSG
KTAVVLTGRTHFYEGRGVESVVHGVRTVAALGATTMILTNGCGGLDPSWGPGTIVAIRDHINLTGATPLRGATFIDMSTA
WTPHLLEVTERVAPGTPTGVYAQFRGPQYETPAEVRMARTIGADLVGMSTALEAIAAREAGLDLLGLSLVTNHAAGVTDA
PLDHAEVIAAGKQAAPRLASLLADITAAI

Specific function: Cleavage of guanosine or inosine to respective bases and sugar-1-phosphate molecules [H]

COG id: COG0005

COG function: function code F; Purine nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/MTAP phosphorylase family [H]

Homologues:

Organism=Homo sapiens, GI157168362, Length=257, Percent_Identity=37.3540856031128, Blast_Score=140, Evalue=1e-33,
Organism=Escherichia coli, GI1788746, Length=249, Percent_Identity=36.144578313253, Blast_Score=154, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17541190, Length=276, Percent_Identity=34.7826086956522, Blast_Score=134, Evalue=5e-32,
Organism=Saccharomyces cerevisiae, GI6323238, Length=250, Percent_Identity=32, Blast_Score=111, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24656093, Length=286, Percent_Identity=29.7202797202797, Blast_Score=118, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24656090, Length=284, Percent_Identity=29.5774647887324, Blast_Score=118, Evalue=5e-27,
Organism=Drosophila melanogaster, GI45552887, Length=282, Percent_Identity=29.4326241134752, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI45552885, Length=282, Percent_Identity=29.4326241134752, Blast_Score=117, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24762376, Length=221, Percent_Identity=29.4117647058824, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011268
- InterPro:   IPR000845
- InterPro:   IPR011269
- InterPro:   IPR001369
- InterPro:   IPR018099 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =2.4.2.1 [H]

Molecular weight: Translated: 27730; Mature: 27599

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01240 PNP_MTAP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSPEFTDPQAIARNAADAIRSRLSLTSVDMGLVLGSGWSAGVDRLGKTLGRIPLSELPG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHEEECCCCHHHHHHHHHHHHCCCHHHCCC
FFAPVVAGHDGDLVAVALPSGKTAVVLTGRTHFYEGRGVESVVHGVRTVAALGATTMILT
HHHHHHCCCCCCEEEEEECCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHEEEEE
NGCGGLDPSWGPGTIVAIRDHINLTGATPLRGATFIDMSTAWTPHLLEVTERVAPGTPTG
CCCCCCCCCCCCCEEEEEECCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCC
VYAQFRGPQYETPAEVRMARTIGADLVGMSTALEAIAAREAGLDLLGLSLVTNHAAGVTD
EEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
APLDHAEVIAAGKQAAPRLASLLADITAAI
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TSPEFTDPQAIARNAADAIRSRLSLTSVDMGLVLGSGWSAGVDRLGKTLGRIPLSELPG
CCCCCCCHHHHHHHHHHHHHHHHHHHHEEEHEEECCCCHHHHHHHHHHHHCCCHHHCCC
FFAPVVAGHDGDLVAVALPSGKTAVVLTGRTHFYEGRGVESVVHGVRTVAALGATTMILT
HHHHHHCCCCCCEEEEEECCCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHCHHEEEEE
NGCGGLDPSWGPGTIVAIRDHINLTGATPLRGATFIDMSTAWTPHLLEVTERVAPGTPTG
CCCCCCCCCCCCCEEEEEECCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHCCCCCCC
VYAQFRGPQYETPAEVRMARTIGADLVGMSTALEAIAAREAGLDLLGLSLVTNHAAGVTD
EEEEECCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCC
APLDHAEVIAAGKQAAPRLASLLADITAAI
CCCCHHHHHHCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10600382 [H]