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The map label for this gene is metK [H]

Identifier: 50843058

GI number: 50843058

Start: 1713357

End: 1714547

Strand: Reverse

Name: metK [H]

Synonym: PPA1587

Alternate gene names: 50843058

Gene position: 1714547-1713357 (Counterclockwise)

Preceding gene: 50843059

Following gene: 50843057

Centisome position: 66.97

GC content: 66.08

Gene sequence:

>1191_bases
ATGTGTTCGATTCGTACTGCCGAGTCTGTGTGTGCTGGTCATCCGGACAAGTTGGCCGATCAGATCGCCGACCAGATCCT
CGACGACATCCTCTACGACGACAAGGCCGCACGGGTGGCGGTGGAGGTGATGGCAGCCGGTCGGCGGATCATCGTCACCG
GCGAAATCACCACCAACCACCGCCCGCGTATCCGGGAGTCTGTGCGCACCGCCCTCGCGCGTGCCGGATACAGTCCGCTC
GGGATCCTGATCTACGTGTGGACGCGCCGCCAATCGAGCGACATCAACGCCGGGGTCACCACCTCGCTGGAGGCACGCGG
TGGTGATAGTTCGGCGTTCGCGTTGCAGGGGGCTGGCGATCAGGGCACGGTCTACGGTTACGCCACCGCCGAGACCCCTG
AACGCCTCCCGCTCCCGCTCGTCCTGGCACATCGCATCTGCGAGCGTCTCGATACTGCCCAGGTCGAGGGGACGATCTGC
GGGATCAAGTCGGACGGCAAGGCCCAGGTGAGTGTGCGTTATGACGACACCGGTGCCCCCGCCGCCGTCGAGACCGTCGT
GGTCTCGGTGCAGCATGAGGCGGGCAAGGATCTGGCCGTGTTGGAGCGTGAGGTGCGCTCGCTGATTGTGGCCCCGGCCT
GCCAGACCTACCTACCCATAGACGAGCACACCGAAATTCTTGTGAATCCGTCGGGTCGGTTCGTTGAGGGCGGGCCGCGA
GCCGATACCGGGCTGACGGGCCGGAAGCTCATGGTCGGCACCTACGGCGGGCTCGGGTCTCATGGTGGGGGCGCGTTCTC
CGGCAAGGACCCATCCAAGGTCGACCGGTCAGCGGCCTACATGGCTCGCCTGATCGCTCGTACGATCGTTGACGCCGGGC
TGGCCGCTGAGTGCGAGGTGGGCATCTCGTATGCGATTGGGAAGGCTGATCCGGTCGCGTTCGAGGTGGACACGCTCGGC
ACCGGCGAGTACGCCGACCACATCTTGACCGCCGCCGCACGTGACGTGTTCGTGCTGCGGCCGGCGGGGATCATCGACGT
CCTCAACCTGCGCACACCCCGTTACCGGGATCTCGCGGTCTACGGGCACATGGGCCGGGATTGGCCGCGCTGGGAACAAA
CCTGGCGTTTTGAGCGCGACCTGCGGAAGGCGGTGGAGCCCCGTGCGCATCGAGCAGCTACCCATCGCTGA

Upstream 100 bases:

>100_bases
CAACGCCCGCTCCTAACCGCCCCTCATGATCTTCGCGTCCACCCTCTAGTGGGGTGGACGCTTTCTTGTTCCCCTCCGAT
TCGTGGAAGCGAGTGTGCTT

Downstream 100 bases:

>100_bases
CCTCACGCCAGCCGACTACAACCCGCGCAAAGACCTCCGGCCCGGCGACCCCGACTACGAAAAGCTCAAGCGGTCGCTGA
CGGAGTTCGGATACGTCGAA

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MCSIRTAESVCAGHPDKLADQIADQILDDILYDDKAARVAVEVMAAGRRIIVTGEITTNHRPRIRESVRTALARAGYSPL
GILIYVWTRRQSSDINAGVTTSLEARGGDSSAFALQGAGDQGTVYGYATAETPERLPLPLVLAHRICERLDTAQVEGTIC
GIKSDGKAQVSVRYDDTGAPAAVETVVVSVQHEAGKDLAVLEREVRSLIVAPACQTYLPIDEHTEILVNPSGRFVEGGPR
ADTGLTGRKLMVGTYGGLGSHGGGAFSGKDPSKVDRSAAYMARLIARTIVDAGLAAECEVGISYAIGKADPVAFEVDTLG
TGEYADHILTAAARDVFVLRPAGIIDVLNLRTPRYRDLAVYGHMGRDWPRWEQTWRFERDLRKAVEPRAHRAATHR

Sequences:

>Translated_396_residues
MCSIRTAESVCAGHPDKLADQIADQILDDILYDDKAARVAVEVMAAGRRIIVTGEITTNHRPRIRESVRTALARAGYSPL
GILIYVWTRRQSSDINAGVTTSLEARGGDSSAFALQGAGDQGTVYGYATAETPERLPLPLVLAHRICERLDTAQVEGTIC
GIKSDGKAQVSVRYDDTGAPAAVETVVVSVQHEAGKDLAVLEREVRSLIVAPACQTYLPIDEHTEILVNPSGRFVEGGPR
ADTGLTGRKLMVGTYGGLGSHGGGAFSGKDPSKVDRSAAYMARLIARTIVDAGLAAECEVGISYAIGKADPVAFEVDTLG
TGEYADHILTAAARDVFVLRPAGIIDVLNLRTPRYRDLAVYGHMGRDWPRWEQTWRFERDLRKAVEPRAHRAATHR
>Mature_396_residues
MCSIRTAESVCAGHPDKLADQIADQILDDILYDDKAARVAVEVMAAGRRIIVTGEITTNHRPRIRESVRTALARAGYSPL
GILIYVWTRRQSSDINAGVTTSLEARGGDSSAFALQGAGDQGTVYGYATAETPERLPLPLVLAHRICERLDTAQVEGTIC
GIKSDGKAQVSVRYDDTGAPAAVETVVVSVQHEAGKDLAVLEREVRSLIVAPACQTYLPIDEHTEILVNPSGRFVEGGPR
ADTGLTGRKLMVGTYGGLGSHGGGAFSGKDPSKVDRSAAYMARLIARTIVDAGLAAECEVGISYAIGKADPVAFEVDTLG
TGEYADHILTAAARDVFVLRPAGIIDVLNLRTPRYRDLAVYGHMGRDWPRWEQTWRFERDLRKAVEPRAHRAATHR

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=43.1578947368421, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=42.1052631578947, Blast_Score=235, Evalue=6e-62,
Organism=Escherichia coli, GI1789311, Length=381, Percent_Identity=45.1443569553806, Blast_Score=302, Evalue=2e-83,
Organism=Caenorhabditis elegans, GI17538498, Length=380, Percent_Identity=41.3157894736842, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17551082, Length=383, Percent_Identity=41.77545691906, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI17538494, Length=389, Percent_Identity=40.3598971722365, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI25145633, Length=391, Percent_Identity=41.1764705882353, Blast_Score=241, Evalue=5e-64,
Organism=Caenorhabditis elegans, GI32565905, Length=382, Percent_Identity=38.2198952879581, Blast_Score=218, Evalue=6e-57,
Organism=Caenorhabditis elegans, GI32565911, Length=334, Percent_Identity=40.7185628742515, Blast_Score=205, Evalue=3e-53,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=40.5797101449275, Blast_Score=203, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI32565903, Length=312, Percent_Identity=40.7051282051282, Blast_Score=197, Evalue=7e-51,
Organism=Saccharomyces cerevisiae, GI6320710, Length=387, Percent_Identity=42.3772609819121, Blast_Score=251, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6323209, Length=386, Percent_Identity=41.9689119170984, Blast_Score=246, Evalue=6e-66,
Organism=Drosophila melanogaster, GI24580527, Length=392, Percent_Identity=41.0714285714286, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI24580525, Length=392, Percent_Identity=41.0714285714286, Blast_Score=240, Evalue=1e-63,
Organism=Drosophila melanogaster, GI45552159, Length=390, Percent_Identity=41.5384615384615, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24580529, Length=390, Percent_Identity=41.5384615384615, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24580537, Length=390, Percent_Identity=41.5384615384615, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24580533, Length=390, Percent_Identity=41.5384615384615, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24580535, Length=390, Percent_Identity=41.5384615384615, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24580531, Length=390, Percent_Identity=41.5384615384615, Blast_Score=237, Evalue=8e-63,
Organism=Drosophila melanogaster, GI24580541, Length=380, Percent_Identity=40.5263157894737, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 42714; Mature: 42714

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCSIRTAESVCAGHPDKLADQIADQILDDILYDDKAARVAVEVMAAGRRIIVTGEITTNH
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCC
RPRIRESVRTALARAGYSPLGILIYVWTRRQSSDINAGVTTSLEARGGDSSAFALQGAGD
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEECCCC
QGTVYGYATAETPERLPLPLVLAHRICERLDTAQVEGTICGIKSDGKAQVSVRYDDTGAP
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEECCCCCEEEEEEECCCCCC
AAVETVVVSVQHEAGKDLAVLEREVRSLIVAPACQTYLPIDEHTEILVNPSGRFVEGGPR
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCEECCCCC
ADTGLTGRKLMVGTYGGLGSHGGGAFSGKDPSKVDRSAAYMARLIARTIVDAGLAAECEV
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GISYAIGKADPVAFEVDTLGTGEYADHILTAAARDVFVLRPAGIIDVLNLRTPRYRDLAV
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCEEHHHCCCCCCCEEEE
YGHMGRDWPRWEQTWRFERDLRKAVEPRAHRAATHR
ECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC
>Mature Secondary Structure
MCSIRTAESVCAGHPDKLADQIADQILDDILYDDKAARVAVEVMAAGRRIIVTGEITTNH
CCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEEEEEECCCC
RPRIRESVRTALARAGYSPLGILIYVWTRRQSSDINAGVTTSLEARGGDSSAFALQGAGD
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEEEECCCCCCCEEEEECCCC
QGTVYGYATAETPERLPLPLVLAHRICERLDTAQVEGTICGIKSDGKAQVSVRYDDTGAP
CCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEEECCCCCEEEEEEECCCCCC
AAVETVVVSVQHEAGKDLAVLEREVRSLIVAPACQTYLPIDEHTEILVNPSGRFVEGGPR
CEEEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECCCCCEECCCCC
ADTGLTGRKLMVGTYGGLGSHGGGAFSGKDPSKVDRSAAYMARLIARTIVDAGLAAECEV
CCCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEC
GISYAIGKADPVAFEVDTLGTGEYADHILTAAARDVFVLRPAGIIDVLNLRTPRYRDLAV
CEEEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCEEEECCCCCEEHHHCCCCCCCEEEE
YGHMGRDWPRWEQTWRFERDLRKAVEPRAHRAATHR
ECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA