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The map label for this gene is tsf

Identifier: 50842998

GI number: 50842998

Start: 1642256

End: 1643068

Strand: Reverse

Name: tsf

Synonym: PPA1520

Alternate gene names: 50842998

Gene position: 1643068-1642256 (Counterclockwise)

Preceding gene: 50842999

Following gene: 50842997

Centisome position: 64.18

GC content: 60.89

Gene sequence:

>813_bases
ATGGCTATCACTGCTGCTGAGGTCAAGAAACTGCGCGACGCTACTGGCGCCGGCATGATGGACGCCAAGAAGGCCCTCAC
TGAGGCCGATGGCGACTTCGATAAGGCCGTTGACATCTTGCGTGTTTCCGGCGCTGCTAAGGCTGCGAAGCGTTCCGACC
GTGAGGCCAGCAACGGCCTGGTTGCTGCCGCTGGCTCTTCCCTGGTGCATATCGGCTCTGAGACCGACTTCGTCGCCAAG
AATGAGGAGTTCATCGCCGCCGCTCACGAGATCGCCGAGGCTGCTGATAAGGCCGGGGCTGACTCTAAGGATGCTGCTAA
TGCCGCAGCTTTGGCTGATGGCACCACTGTTGGTGACAAGCTTGGTGAGCTCGCCGCCAAAATCGGTGAGAAGATTGAGC
TCGCTAACGCCGCTCACTTCGATGGCAACGCGCACGTGTACCTCCACCGTCGTTCCCAGGACTTGCCCCCGCAGGTCGGC
GTCATGGTTGAGTACGAGGGCGACAACATCGAGGCTGTCCACGGCGTTTGCCTGCAGATCGCGGCTATGAGCCCGCGCTG
GCTGTCCCGTGACGAGGTCCCTGCCGACGTCGTCGAGCACGAGCGCACCGTTGCCGCTGATATTGCCCGCGAGGAGGGTA
AGCCTGAAAAGATCATCGACCGCATCGTCGAGGGTCGCCTAGGCGGCTTCTTCAAGGAGAACTGCCTGCTCGATCAGCCT
GCCATTTCCGATGACAAGAAAACCGTCGCCCAGACCCTTGAGGCCGCTGGTGTCACCCTCAAGCGTTTCGTTCGCTTTTC
TGCCGGAGAGTGA

Upstream 100 bases:

>100_bases
GCTGAAGAGCCGAAGGCCGAGGCCAAGAAGGCTGACGAGGCCCCCGAGGCCGAGAAGTCCAACTGAAGTTCGCCCACGTC
TAGACAGGACTGAGATATCT

Downstream 100 bases:

>100_bases
TCTCGTCCCCACGTGGGGATGATAACGAGATTGTGACGACGAACGGTCCCACCGTACGAGGTGGGGCCGTTCGTCGTTTC
CCAGCGAATGACGGTCGCGG

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts

Number of amino acids: Translated: 270; Mature: 269

Protein sequence:

>270_residues
MAITAAEVKKLRDATGAGMMDAKKALTEADGDFDKAVDILRVSGAAKAAKRSDREASNGLVAAAGSSLVHIGSETDFVAK
NEEFIAAAHEIAEAADKAGADSKDAANAAALADGTTVGDKLGELAAKIGEKIELANAAHFDGNAHVYLHRRSQDLPPQVG
VMVEYEGDNIEAVHGVCLQIAAMSPRWLSRDEVPADVVEHERTVAADIAREEGKPEKIIDRIVEGRLGGFFKENCLLDQP
AISDDKKTVAQTLEAAGVTLKRFVRFSAGE

Sequences:

>Translated_270_residues
MAITAAEVKKLRDATGAGMMDAKKALTEADGDFDKAVDILRVSGAAKAAKRSDREASNGLVAAAGSSLVHIGSETDFVAK
NEEFIAAAHEIAEAADKAGADSKDAANAAALADGTTVGDKLGELAAKIGEKIELANAAHFDGNAHVYLHRRSQDLPPQVG
VMVEYEGDNIEAVHGVCLQIAAMSPRWLSRDEVPADVVEHERTVAADIAREEGKPEKIIDRIVEGRLGGFFKENCLLDQP
AISDDKKTVAQTLEAAGVTLKRFVRFSAGE
>Mature_269_residues
AITAAEVKKLRDATGAGMMDAKKALTEADGDFDKAVDILRVSGAAKAAKRSDREASNGLVAAAGSSLVHIGSETDFVAKN
EEFIAAAHEIAEAADKAGADSKDAANAAALADGTTVGDKLGELAAKIGEKIELANAAHFDGNAHVYLHRRSQDLPPQVGV
MVEYEGDNIEAVHGVCLQIAAMSPRWLSRDEVPADVVEHERTVAADIAREEGKPEKIIDRIVEGRLGGFFKENCLLDQPA
ISDDKKTVAQTLEAAGVTLKRFVRFSAGE

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family

Homologues:

Organism=Escherichia coli, GI1786366, Length=277, Percent_Identity=37.1841155234657, Blast_Score=152, Evalue=2e-38,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): EFTS_PROAC (Q6A7J8)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056225.1
- ProteinModelPortal:   Q6A7J8
- SMR:   Q6A7J8
- GeneID:   2931532
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1520
- NMPDR:   fig|267747.1.peg.1502
- HOGENOM:   HBG713289
- OMA:   YLHGTRI
- ProtClustDB:   PRK09377
- BioCyc:   PACN267747:PPA1520-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00050
- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449
- Gene3D:   G3DSA:3.30.479.20
- PANTHER:   PTHR11741
- TIGRFAMs:   TIGR00116

Pfam domain/function: PF00889 EF_TS; PF00627 UBA; SSF54713 EF_TS; SSF46934 UBA_like

EC number: NA

Molecular weight: Translated: 28488; Mature: 28357

Theoretical pI: Translated: 4.76; Mature: 4.76

Prosite motif: PS01126 EF_TS_1; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAITAAEVKKLRDATGAGMMDAKKALTEADGDFDKAVDILRVSGAAKAAKRSDREASNGL
CCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHCCE
VAAAGSSLVHIGSETDFVAKNEEFIAAAHEIAEAADKAGADSKDAANAAALADGTTVGDK
EEECCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCHHHHH
LGELAAKIGEKIELANAAHFDGNAHVYLHRRSQDLPPQVGVMVEYEGDNIEAVHGVCLQI
HHHHHHHHCCCEECCCCEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHH
AAMSPRWLSRDEVPADVVEHERTVAADIAREEGKPEKIIDRIVEGRLGGFFKENCLLDQP
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCCC
AISDDKKTVAQTLEAAGVTLKRFVRFSAGE
CCCCHHHHHHHHHHHHCHHHHHHHHHCCCC
>Mature Secondary Structure 
AITAAEVKKLRDATGAGMMDAKKALTEADGDFDKAVDILRVSGAAKAAKRSDREASNGL
CCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHHHCCCHHHCCE
VAAAGSSLVHIGSETDFVAKNEEFIAAAHEIAEAADKAGADSKDAANAAALADGTTVGDK
EEECCCCEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCHHHHH
LGELAAKIGEKIELANAAHFDGNAHVYLHRRSQDLPPQVGVMVEYEGDNIEAVHGVCLQI
HHHHHHHHCCCEECCCCEECCCCEEEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHHH
AAMSPRWLSRDEVPADVVEHERTVAADIAREEGKPEKIIDRIVEGRLGGFFKENCLLDQP
HHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCHHHCCCCCCCC
AISDDKKTVAQTLEAAGVTLKRFVRFSAGE
CCCCHHHHHHHHHHHHCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA