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The map label for this gene is ispG

Identifier: 50842984

GI number: 50842984

Start: 1628291

End: 1629433

Strand: Reverse

Name: ispG

Synonym: PPA1506

Alternate gene names: 50842984

Gene position: 1629433-1628291 (Counterclockwise)

Preceding gene: 50842985

Following gene: 50842983

Centisome position: 63.64

GC content: 59.41

Gene sequence:

>1143_bases
ATGACCGTGAACCTGGGTATGCCTACCCCTCCCATTCCGACCCTGGCGCCACGTCGTAAGACGCACCAGATCAAGGTGGG
AGATGTGCTCGTCGGTGGTGACGCACCCATTTCAGTGCAGTCCATGACAACCACCAAGACTCACGACGTTGGTGCCACCC
TGCAGCAGATCGCCGCCTTGACCGCTGCAGGTTGTGACATCGTACGCGTCGCATGCCCAACCGATAAGGACGCCGAGGTG
CTACCGATCATCGCCAAACGGTCCCAGATCCCGGTGATTGCCGATATCCACTTCCAGCCGAAGTACGTCTTCCAAGCGAT
CGAGGCTGGCTGTGGCGCGGTGCGCGTCAACCCCGGAAATATCCGCAAGTTCGACGACCAGATCGAATCGATTTGTCAGG
CTGCCACCGAGCACGGTACGAGCATCCGAATCGGCGTGAATGCTGGGTCTCTCGACAAACGTCTGCTTGACAAATACGGA
GCCCCGACCGCCGAGGCCATGGTGGAGTCGGCGCTGTGGGAGGCTAGCCTCTTTGAGCAATACGGATTCCGGGATTTCAA
AATATCGGTGAAACACCACGACCCGGTTGTCATGATCCGTGCCTATGAACAGCTCGCCGCCAAATGCGATTATCCCCTTC
ATCTGGGCGTTACTGAGGCTGGTCCGGCCTTCCAAGGCACCATCAAGTCGGCGGTGGCCTTCGGGCACCTCCTTGCCGAG
GGTATCGGCGACACCATACGCGTCTCCTTGTCGGCTGATCCGGTCGAGGAAGTCAAGGTGGGTATCAAGATCCTGGAGTC
GCTCAACCTACGTCCTCGAGGTCTAGAGATCGTCTCCTGCCCCTCTTGCGGACGTTGCCAGGTCGATGTTCTAACGCTGG
CCAATGACGTTACTGATGCCCTTGAAGGGATTGATGCTCCGCTGCGCGTCGCTGTCATGGGTTGCGTCGTCAATGGGCTC
GGAGAAGGCCGCGAAGCTGACCTGGGTGTTGCCGCCGGCAATGGCAAGGGCAAGATCTTCAAGCATGGCGAGGTCATCCG
TACCGTTCCGGAAGGCGAGATCGTTCAGTTCCTCGTTCAGGAGGCCAACCGGATGGCCGACGAGATGGACACCACCGGGG
CCGTTGAGGTCACGGTCTCCTGA

Upstream 100 bases:

>100_bases
CCGACGACGAGGACTGAGTGCTCGCTGTCAGCGTCACTACAGCGGCGACGGTGGTGACACAATGGAGACGACAACCCGCG
ATTTCAGGAGTGACACGTCA

Downstream 100 bases:

>100_bases
GGTGAGGACCCGCGTCAGGCAGCTCGACGACGCCGACCTGAGCCAGACCATCGAGTTCCTCTCGCGTGCCCCGGTCGCCA
ACGTCTTCCTGCTGTCGAGG

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MTVNLGMPTPPIPTLAPRRKTHQIKVGDVLVGGDAPISVQSMTTTKTHDVGATLQQIAALTAAGCDIVRVACPTDKDAEV
LPIIAKRSQIPVIADIHFQPKYVFQAIEAGCGAVRVNPGNIRKFDDQIESICQAATEHGTSIRIGVNAGSLDKRLLDKYG
APTAEAMVESALWEASLFEQYGFRDFKISVKHHDPVVMIRAYEQLAAKCDYPLHLGVTEAGPAFQGTIKSAVAFGHLLAE
GIGDTIRVSLSADPVEEVKVGIKILESLNLRPRGLEIVSCPSCGRCQVDVLTLANDVTDALEGIDAPLRVAVMGCVVNGL
GEGREADLGVAAGNGKGKIFKHGEVIRTVPEGEIVQFLVQEANRMADEMDTTGAVEVTVS

Sequences:

>Translated_380_residues
MTVNLGMPTPPIPTLAPRRKTHQIKVGDVLVGGDAPISVQSMTTTKTHDVGATLQQIAALTAAGCDIVRVACPTDKDAEV
LPIIAKRSQIPVIADIHFQPKYVFQAIEAGCGAVRVNPGNIRKFDDQIESICQAATEHGTSIRIGVNAGSLDKRLLDKYG
APTAEAMVESALWEASLFEQYGFRDFKISVKHHDPVVMIRAYEQLAAKCDYPLHLGVTEAGPAFQGTIKSAVAFGHLLAE
GIGDTIRVSLSADPVEEVKVGIKILESLNLRPRGLEIVSCPSCGRCQVDVLTLANDVTDALEGIDAPLRVAVMGCVVNGL
GEGREADLGVAAGNGKGKIFKHGEVIRTVPEGEIVQFLVQEANRMADEMDTTGAVEVTVS
>Mature_379_residues
TVNLGMPTPPIPTLAPRRKTHQIKVGDVLVGGDAPISVQSMTTTKTHDVGATLQQIAALTAAGCDIVRVACPTDKDAEVL
PIIAKRSQIPVIADIHFQPKYVFQAIEAGCGAVRVNPGNIRKFDDQIESICQAATEHGTSIRIGVNAGSLDKRLLDKYGA
PTAEAMVESALWEASLFEQYGFRDFKISVKHHDPVVMIRAYEQLAAKCDYPLHLGVTEAGPAFQGTIKSAVAFGHLLAEG
IGDTIRVSLSADPVEEVKVGIKILESLNLRPRGLEIVSCPSCGRCQVDVLTLANDVTDALEGIDAPLRVAVMGCVVNGLG
EGREADLGVAAGNGKGKIFKHGEVIRTVPEGEIVQFLVQEANRMADEMDTTGAVEVTVS

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family

Homologues:

Organism=Escherichia coli, GI1788863, Length=360, Percent_Identity=44.1666666666667, Blast_Score=309, Evalue=2e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPG_PROAC (Q6A7L2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056211.1
- ProteinModelPortal:   Q6A7L2
- GeneID:   2932950
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1506
- NMPDR:   fig|267747.1.peg.1488
- HOGENOM:   HBG335271
- OMA:   APIVVQS
- ProtClustDB:   PRK00366
- BioCyc:   PACN267747:PPA1506-MONOMER
- BRENDA:   1.17.4.3
- HAMAP:   MF_00159
- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF004640
- TIGRFAMs:   TIGR00612

Pfam domain/function: PF04551 GcpE; SSF51717 DHP_synth_like

EC number: =1.17.7.1

Molecular weight: Translated: 40392; Mature: 40261

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVNLGMPTPPIPTLAPRRKTHQIKVGDVLVGGDAPISVQSMTTTKTHDVGATLQQIAAL
CEEECCCCCCCCCCCCCCCCCCEEEECCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHH
TAAGCDIVRVACPTDKDAEVLPIIAKRSQIPVIADIHFQPKYVFQAIEAGCGAVRVNPGN
HHCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCC
IRKFDDQIESICQAATEHGTSIRIGVNAGSLDKRLLDKYGAPTAEAMVESALWEASLFEQ
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YGFRDFKISVKHHDPVVMIRAYEQLAAKCDYPLHLGVTEAGPAFQGTIKSAVAFGHLLAE
HCCEEEEEEEECCCCEEEEEEHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
GIGDTIRVSLSADPVEEVKVGIKILESLNLRPRGLEIVSCPSCGRCQVDVLTLANDVTDA
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHH
LEGIDAPLRVAVMGCVVNGLGEGREADLGVAAGNGKGKIFKHGEVIRTVPEGEIVQFLVQ
HHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCEEEECCCHHHHHHHHH
EANRMADEMDTTGAVEVTVS
HHHHHHHHCCCCCEEEEEEC
>Mature Secondary Structure 
TVNLGMPTPPIPTLAPRRKTHQIKVGDVLVGGDAPISVQSMTTTKTHDVGATLQQIAAL
EEECCCCCCCCCCCCCCCCCCEEEECCEEECCCCCEEEEEEECCCCCHHHHHHHHHHHH
TAAGCDIVRVACPTDKDAEVLPIIAKRSQIPVIADIHFQPKYVFQAIEAGCGAVRVNPGN
HHCCCCEEEEECCCCCCCCEEEEEECCCCCCEEEEECCCHHHHHHHHHCCCCEEEECCCC
IRKFDDQIESICQAATEHGTSIRIGVNAGSLDKRLLDKYGAPTAEAMVESALWEASLFEQ
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
YGFRDFKISVKHHDPVVMIRAYEQLAAKCDYPLHLGVTEAGPAFQGTIKSAVAFGHLLAE
HCCEEEEEEEECCCCEEEEEEHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
GIGDTIRVSLSADPVEEVKVGIKILESLNLRPRGLEIVSCPSCGRCQVDVLTLANDVTDA
CCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEHHHHHHHH
LEGIDAPLRVAVMGCVVNGLGEGREADLGVAAGNGKGKIFKHGEVIRTVPEGEIVQFLVQ
HHCCCCHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCEEECCCEEEECCCHHHHHHHHH
EANRMADEMDTTGAVEVTVS
HHHHHHHHCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA