Click here to switch to the map view.

The map label for this gene is poxB [H]

Identifier: 50842947

GI number: 50842947

Start: 1586166

End: 1587905

Strand: Reverse

Name: poxB [H]

Synonym: PPA1468

Alternate gene names: 50842947

Gene position: 1587905-1586166 (Counterclockwise)

Preceding gene: 50842948

Following gene: 50842946

Centisome position: 62.02

GC content: 59.2

Gene sequence:

>1740_bases
ATGAATGTTGCCGAACAGCTCGTCCAACAGCTCCTCGATGCGGGGGTGCGGCGGATCTACGGCATCGTCGGTGACTCCCT
CAACCCGGTCGTCGATGCGGTTCGTAAGACGGGCGGCTCCGTCAAAGGCGGCATTGATTGGGTGCACGTCCGTCACGAGG
AGGCCGCGGCCTTCGCAGCCGCTGCAGAGGCGACCTTGACCGGTGAGCTTGCGGTATGTGCGGGCTCCTGCGGGCCGGGC
AACCTGCACCTCATCAATGGTCTGTTCGATGCCTACCGTTCCCAAGTCCCGGTGTTGGCTATCGCATCTCACGTTCCTAG
CGTCCAGATCGGCATGAGCTACTTCCAGGAGACTCACCCGGACCGTCTGTTTGTGGAGTGCTCCGACTACTGTGAGCTGG
TTTCTACCCCGGAGCAGGCTCCGCGCGTCATGAATTCGGCCATCCGCCATGCCGTTGCTGGTAGCACCGTCTCCGTTATC
ACCCTTCCCGGCGACATTTCCGACAAGAAGGCTGCAGCGCCTTCCCCGGTTTTCATCCCGAGTCGTCGGCCGACTATCGA
GCCGAACCCGGAGGACGTCCGTCAACTGGTCAAGCTGCTTAACGACGTCAGGAAAGTCGCCATCTTTGCCGGCGCGGGTG
TCGAGGGCGCTCACGATGAGGTCCTTGCTCTGGCCGACAAGCTCAAGGCCCCTATCGGCCACTCCCTGCGTGGTAAGGAC
TTCATCCAATACGACAACCCCTTTGACGTCGGTATGACCGGCTTGCTGGGCTACGGTGCTGCTGCCGAGGGCATGAATGA
CGCCGATGTTCTCATCCTGCTAGGAACCGATTTCCCCTACAACCAGTTCCTCCCTGATACCCTCACTGTCCAGGTTGAGA
ACCGTCCAGAGAAGCTAGGACGTCGTACTGACGTGTCCTTCCCGATCCATGCCGATGTTAAAGCCCTTTGCGACGCCGTC
ACCCCGCTGCTGAAACAGCATGATGACAAATTCCTTAAGGCCGCAGTTAAGCGCCACGCCAAGATTATGAAGGCCCCGGT
CGGCTCGTACACTCGCAATGTCGAGCATATGAAGCCGATCCACCCCGAGTACGTCGCGCACGTCCTCAATGAAGTAGCTG
ACCGTGATGCCATCTTCACTGCCGATACTGGTATGTGCAATGTGTGGACGGCTCGTTATATCGATCCCCTTGGCACCCGC
CGTCTTATCGGATCCTTCCTGCATGGATCGATGGCCAATGCCCTGCCGCACGCCATTGGCGCCCAGGCTTCCCACCCCGG
GCGTCAGGTTATTTCGGTGTCCGGTGACGGCGGCCTCTCGATGCTCCTTGGAGAGCTCGTGACAGCGCGTATGCTCAACT
TGCCGATCAAGGTGGTTGCCTTCAATAACTCCACTCTCGGCATGGTCAAACTGGAGATGCTCGTGAATGGGTTACCTGAC
TTCGGTACCGACGTCCACGACGTCGACTATGCAGGTCTCGCCAAAGCGATTGGGTTTCACGCCGAGCGGGTTGATGACCC
ACGCAACATTCGCCGTGCCCTAACGGATGCTATGGATTTTGACGGTCCAGCCCTAGTCGAGGTGATCACTGACCCCAACG
CCTTGTCCCTGCCGCCTGAGATCAAGGGCGAGCAGATGATCGGGTTTGCCACCGCCATGAGCAAGATTGTGCTCAATCGT
GGTGCTGGTGAGGCGGTGTCGATGGCCACGTCCAATATGAGGAACATTCCCCGCTTCTGA

Upstream 100 bases:

>100_bases
CGCGCGAAGAGTGAGGTGAGACTAGGACGATAAGTCGTATGCTGGTATGCGGCCGGTCGGCCGCTTGTCCCATGTCGTCA
GTACTGCGGAGGTCCCTTTC

Downstream 100 bases:

>100_bases
CCGTCGCGACTCGGTGGGGCCGTCACCGCAAGGTGGCGGCTCCTCGTCGATTCATGGGACGTGATCCGGGAGACGTATCG
GCCCAGCTGAATCACATGTG

Product: pyruvate dehydrogenase

Products: NA

Alternate protein names: Pyruvate oxidase; POX [H]

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MNVAEQLVQQLLDAGVRRIYGIVGDSLNPVVDAVRKTGGSVKGGIDWVHVRHEEAAAFAAAAEATLTGELAVCAGSCGPG
NLHLINGLFDAYRSQVPVLAIASHVPSVQIGMSYFQETHPDRLFVECSDYCELVSTPEQAPRVMNSAIRHAVAGSTVSVI
TLPGDISDKKAAAPSPVFIPSRRPTIEPNPEDVRQLVKLLNDVRKVAIFAGAGVEGAHDEVLALADKLKAPIGHSLRGKD
FIQYDNPFDVGMTGLLGYGAAAEGMNDADVLILLGTDFPYNQFLPDTLTVQVENRPEKLGRRTDVSFPIHADVKALCDAV
TPLLKQHDDKFLKAAVKRHAKIMKAPVGSYTRNVEHMKPIHPEYVAHVLNEVADRDAIFTADTGMCNVWTARYIDPLGTR
RLIGSFLHGSMANALPHAIGAQASHPGRQVISVSGDGGLSMLLGELVTARMLNLPIKVVAFNNSTLGMVKLEMLVNGLPD
FGTDVHDVDYAGLAKAIGFHAERVDDPRNIRRALTDAMDFDGPALVEVITDPNALSLPPEIKGEQMIGFATAMSKIVLNR
GAGEAVSMATSNMRNIPRF

Sequences:

>Translated_579_residues
MNVAEQLVQQLLDAGVRRIYGIVGDSLNPVVDAVRKTGGSVKGGIDWVHVRHEEAAAFAAAAEATLTGELAVCAGSCGPG
NLHLINGLFDAYRSQVPVLAIASHVPSVQIGMSYFQETHPDRLFVECSDYCELVSTPEQAPRVMNSAIRHAVAGSTVSVI
TLPGDISDKKAAAPSPVFIPSRRPTIEPNPEDVRQLVKLLNDVRKVAIFAGAGVEGAHDEVLALADKLKAPIGHSLRGKD
FIQYDNPFDVGMTGLLGYGAAAEGMNDADVLILLGTDFPYNQFLPDTLTVQVENRPEKLGRRTDVSFPIHADVKALCDAV
TPLLKQHDDKFLKAAVKRHAKIMKAPVGSYTRNVEHMKPIHPEYVAHVLNEVADRDAIFTADTGMCNVWTARYIDPLGTR
RLIGSFLHGSMANALPHAIGAQASHPGRQVISVSGDGGLSMLLGELVTARMLNLPIKVVAFNNSTLGMVKLEMLVNGLPD
FGTDVHDVDYAGLAKAIGFHAERVDDPRNIRRALTDAMDFDGPALVEVITDPNALSLPPEIKGEQMIGFATAMSKIVLNR
GAGEAVSMATSNMRNIPRF
>Mature_579_residues
MNVAEQLVQQLLDAGVRRIYGIVGDSLNPVVDAVRKTGGSVKGGIDWVHVRHEEAAAFAAAAEATLTGELAVCAGSCGPG
NLHLINGLFDAYRSQVPVLAIASHVPSVQIGMSYFQETHPDRLFVECSDYCELVSTPEQAPRVMNSAIRHAVAGSTVSVI
TLPGDISDKKAAAPSPVFIPSRRPTIEPNPEDVRQLVKLLNDVRKVAIFAGAGVEGAHDEVLALADKLKAPIGHSLRGKD
FIQYDNPFDVGMTGLLGYGAAAEGMNDADVLILLGTDFPYNQFLPDTLTVQVENRPEKLGRRTDVSFPIHADVKALCDAV
TPLLKQHDDKFLKAAVKRHAKIMKAPVGSYTRNVEHMKPIHPEYVAHVLNEVADRDAIFTADTGMCNVWTARYIDPLGTR
RLIGSFLHGSMANALPHAIGAQASHPGRQVISVSGDGGLSMLLGELVTARMLNLPIKVVAFNNSTLGMVKLEMLVNGLPD
FGTDVHDVDYAGLAKAIGFHAERVDDPRNIRRALTDAMDFDGPALVEVITDPNALSLPPEIKGEQMIGFATAMSKIVLNR
GAGEAVSMATSNMRNIPRF

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=581, Percent_Identity=24.4406196213425, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787096, Length=574, Percent_Identity=46.5156794425087, Blast_Score=513, Evalue=1e-146,
Organism=Escherichia coli, GI87081685, Length=562, Percent_Identity=27.2241992882562, Blast_Score=185, Evalue=8e-48,
Organism=Escherichia coli, GI1790104, Length=564, Percent_Identity=26.9503546099291, Blast_Score=183, Evalue=3e-47,
Organism=Escherichia coli, GI1786717, Length=561, Percent_Identity=25.3119429590018, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788716, Length=557, Percent_Identity=23.1597845601436, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=568, Percent_Identity=24.4718309859155, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17531301, Length=568, Percent_Identity=24.4718309859155, Blast_Score=83, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=559, Percent_Identity=25.5813953488372, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320816, Length=488, Percent_Identity=21.3114754098361, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19922626, Length=498, Percent_Identity=26.1044176706827, Blast_Score=108, Evalue=8e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =1.2.5.1 [H]

Molecular weight: Translated: 62166; Mature: 62166

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVAEQLVQQLLDAGVRRIYGIVGDSLNPVVDAVRKTGGSVKGGIDWVHVRHEEAAAFAA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHH
AAEATLTGELAVCAGSCGPGNLHLINGLFDAYRSQVPVLAIASHVPSVQIGMSYFQETHP
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHHCCC
DRLFVECSDYCELVSTPEQAPRVMNSAIRHAVAGSTVSVITLPGDISDKKAAAPSPVFIP
CEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEECC
SRRPTIEPNPEDVRQLVKLLNDVRKVAIFAGAGVEGAHDEVLALADKLKAPIGHSLRGKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
FIQYDNPFDVGMTGLLGYGAAAEGMNDADVLILLGTDFPYNQFLPDTLTVQVENRPEKLG
CEECCCCCCCCCHHHHHCCCHHCCCCCCCEEEEEECCCCCHHCCCCEEEEEECCCHHHCC
RRTDVSFPIHADVKALCDAVTPLLKQHDDKFLKAAVKRHAKIMKAPVGSYTRNVEHMKPI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCC
HPEYVAHVLNEVADRDAIFTADTGMCNVWTARYIDPLGTRRLIGSFLHGSMANALPHAIG
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
AQASHPGRQVISVSGDGGLSMLLGELVTARMLNLPIKVVAFNNSTLGMVKLEMLVNGLPD
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHEEHHHHHCCCCC
FGTDVHDVDYAGLAKAIGFHAERVDDPRNIRRALTDAMDFDGPALVEVITDPNALSLPPE
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
IKGEQMIGFATAMSKIVLNRGAGEAVSMATSNMRNIPRF
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNVAEQLVQQLLDAGVRRIYGIVGDSLNPVVDAVRKTGGSVKGGIDWVHVRHEEAAAFAA
CCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEEEHHHHHHHHH
AAEATLTGELAVCAGSCGPGNLHLINGLFDAYRSQVPVLAIASHVPSVQIGMSYFQETHP
HHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEHHHCCCCHHHHHHHHHHCCC
DRLFVECSDYCELVSTPEQAPRVMNSAIRHAVAGSTVSVITLPGDISDKKAAAPSPVFIP
CEEEEEHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEECC
SRRPTIEPNPEDVRQLVKLLNDVRKVAIFAGAGVEGAHDEVLALADKLKAPIGHSLRGKD
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
FIQYDNPFDVGMTGLLGYGAAAEGMNDADVLILLGTDFPYNQFLPDTLTVQVENRPEKLG
CEECCCCCCCCCHHHHHCCCHHCCCCCCCEEEEEECCCCCHHCCCCEEEEEECCCHHHCC
RRTDVSFPIHADVKALCDAVTPLLKQHDDKFLKAAVKRHAKIMKAPVGSYTRNVEHMKPI
CCCCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCHHHCCHHHCCCC
HPEYVAHVLNEVADRDAIFTADTGMCNVWTARYIDPLGTRRLIGSFLHGSMANALPHAIG
CHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHC
AQASHPGRQVISVSGDGGLSMLLGELVTARMLNLPIKVVAFNNSTLGMVKLEMLVNGLPD
CCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCHHEEHHHHHCCCCC
FGTDVHDVDYAGLAKAIGFHAERVDDPRNIRRALTDAMDFDGPALVEVITDPNALSLPPE
CCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCC
IKGEQMIGFATAMSKIVLNRGAGEAVSMATSNMRNIPRF
CCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3016647; 8905232; 9278503; 2663858; 3902830; 8022274 [H]