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The map label for this gene is purR [H]

Identifier: 50842936

GI number: 50842936

Start: 1572942

End: 1573952

Strand: Reverse

Name: purR [H]

Synonym: PPA1457

Alternate gene names: 50842936

Gene position: 1573952-1572942 (Counterclockwise)

Preceding gene: 50842937

Following gene: 50842932

Centisome position: 61.48

GC content: 59.05

Gene sequence:

>1011_bases
ATGGACAGGACGACGATCAAGGACATCGCCAGGGTTGCCGGTGTGTCCCTTGGCGCAGTCTCCTTCGCCCTTAATGACAA
GCCGGGCGTCTCCGATTCCACGAGAGCCCGGGTGAAGGCAGTGGCCAAGGACCTGGGGTGGCGTCCCAATGTGGCTGCGA
GAGCGCTATCGGCCAATCGGGCTGGAGCCGTCGGGCTCGTCATCGCGCGCGACGATCCCAGTTTTGCTGGTGAAGGCTTC
TTCCTCCACCTTATTGCTGGCATGGAGGAGATCCTCACTCCCGCACGTACTGCCTTGGTGCTGCAGTTGGTCGCATCACG
TGAGGAGGAATGCGAGGTCCATCGTCAATGGTGGGCGCAGCGACGGGTTGACGGTGTGGTCCTAGTCGATCCGGCTCGCA
ATGATCCACGGTGGCCGCTCATGGCAGAGATCAAGATGCCGTGCACGGTTGTTGGCGCTATGAGTGACACACCGTTGCCT
GGTGTCCATATCGATGACGCAGCTGCTGCCGGAAACGTCGTCACTCACTTGATTAATCAAGGTCATCGTCGTATTGCCCA
CATCGGTGGCGACCCCACTCTCGAACATAGCATGGTGCGGCGACGAGCCTTTGCTCAGGAAGCCGAGAGACTGGGAGCTG
TTGTTATCGAGGCAGCAGCAACGGATTATTCGGAACAGTCTGGGCTGCGAGAGACCAATGCGTTGCTGAAGACTGAAGAT
CGTCCTACCGCGATCGTCTATGACAATGAGGTGCTGACGCTGGGCGGTTTGGCTGCCGTTGCCGAGCACCGGTTACAGGT
TCCCACTGACATTTCGGTGGTGTCGTTTGAGGACTCACCAGCATGTCGGGTGATGTCGCCAGGAATCACCGCTTTGCGAC
GAGATCCGTCGGTTCTGGGCCGGGATGCTATCACCATGCTGATGGGAATCCTTGGCGGTGACGACGTCGAGGATGTTACC
GAGCAACCCCCTGAACTCATCGTGAGAGGATCCACTGGCCAGGCCCAGTAA

Upstream 100 bases:

>100_bases
TTTAAAATGATGCCGCGCGGGGTCGAGGCAATGAGCCTCGGGCGATGACGCCCCTAGGCCCCCGACAGGGCATTCATGCT
TCGAGAAGGACCGTGACTTC

Downstream 100 bases:

>100_bases
CGGGCGACGCGGCGGTGGTGTCAACCCTTGACCCCGCCACTCTCAACACCGCGGAAGAACACCTTCTGCATCGACGCGAA
GATGATAATGATCGGGATAA

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MDRTTIKDIARVAGVSLGAVSFALNDKPGVSDSTRARVKAVAKDLGWRPNVAARALSANRAGAVGLVIARDDPSFAGEGF
FLHLIAGMEEILTPARTALVLQLVASREEECEVHRQWWAQRRVDGVVLVDPARNDPRWPLMAEIKMPCTVVGAMSDTPLP
GVHIDDAAAAGNVVTHLINQGHRRIAHIGGDPTLEHSMVRRRAFAQEAERLGAVVIEAAATDYSEQSGLRETNALLKTED
RPTAIVYDNEVLTLGGLAAVAEHRLQVPTDISVVSFEDSPACRVMSPGITALRRDPSVLGRDAITMLMGILGGDDVEDVT
EQPPELIVRGSTGQAQ

Sequences:

>Translated_336_residues
MDRTTIKDIARVAGVSLGAVSFALNDKPGVSDSTRARVKAVAKDLGWRPNVAARALSANRAGAVGLVIARDDPSFAGEGF
FLHLIAGMEEILTPARTALVLQLVASREEECEVHRQWWAQRRVDGVVLVDPARNDPRWPLMAEIKMPCTVVGAMSDTPLP
GVHIDDAAAAGNVVTHLINQGHRRIAHIGGDPTLEHSMVRRRAFAQEAERLGAVVIEAAATDYSEQSGLRETNALLKTED
RPTAIVYDNEVLTLGGLAAVAEHRLQVPTDISVVSFEDSPACRVMSPGITALRRDPSVLGRDAITMLMGILGGDDVEDVT
EQPPELIVRGSTGQAQ
>Mature_336_residues
MDRTTIKDIARVAGVSLGAVSFALNDKPGVSDSTRARVKAVAKDLGWRPNVAARALSANRAGAVGLVIARDDPSFAGEGF
FLHLIAGMEEILTPARTALVLQLVASREEECEVHRQWWAQRRVDGVVLVDPARNDPRWPLMAEIKMPCTVVGAMSDTPLP
GVHIDDAAAAGNVVTHLINQGHRRIAHIGGDPTLEHSMVRRRAFAQEAERLGAVVIEAAATDYSEQSGLRETNALLKTED
RPTAIVYDNEVLTLGGLAAVAEHRLQVPTDISVVSFEDSPACRVMSPGITALRRDPSVLGRDAITMLMGILGGDDVEDVT
EQPPELIVRGSTGQAQ

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=339, Percent_Identity=28.9085545722714, Blast_Score=124, Evalue=6e-30,
Organism=Escherichia coli, GI1790194, Length=335, Percent_Identity=28.3582089552239, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1787948, Length=333, Percent_Identity=27.3273273273273, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1786540, Length=338, Percent_Identity=25.1479289940828, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789202, Length=299, Percent_Identity=27.7591973244147, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789456, Length=313, Percent_Identity=27.4760383386581, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1787580, Length=313, Percent_Identity=22.0447284345048, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1789068, Length=340, Percent_Identity=27.0588235294118, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1788474, Length=341, Percent_Identity=24.633431085044, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1790689, Length=347, Percent_Identity=27.0893371757925, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787906, Length=275, Percent_Identity=30.5454545454545, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI48994940, Length=295, Percent_Identity=23.728813559322, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36018; Mature: 36018

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRTTIKDIARVAGVSLGAVSFALNDKPGVSDSTRARVKAVAKDLGWRPNVAARALSANR
CCCCHHHHHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCC
AGAVGLVIARDDPSFAGEGFFLHLIAGMEEILTPARTALVLQLVASREEECEVHRQWWAQ
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRVDGVVLVDPARNDPRWPLMAEIKMPCTVVGAMSDTPLPGVHIDDAAAAGNVVTHLINQ
HCCCCEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHC
GHRRIAHIGGDPTLEHSMVRRRAFAQEAERLGAVVIEAAATDYSEQSGLRETNALLKTED
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCHHHCCCHHHHHHHCCCC
RPTAIVYDNEVLTLGGLAAVAEHRLQVPTDISVVSFEDSPACRVMSPGITALRRDPSVLG
CCEEEEECCCEEEECHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCCHHHHHCCCHHHH
RDAITMLMGILGGDDVEDVTEQPPELIVRGSTGQAQ
HHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCC
>Mature Secondary Structure
MDRTTIKDIARVAGVSLGAVSFALNDKPGVSDSTRARVKAVAKDLGWRPNVAARALSANR
CCCCHHHHHHHHHCCHHHEEEEEECCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCC
AGAVGLVIARDDPSFAGEGFFLHLIAGMEEILTPARTALVLQLVASREEECEVHRQWWAQ
CCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
RRVDGVVLVDPARNDPRWPLMAEIKMPCTVVGAMSDTPLPGVHIDDAAAAGNVVTHLINQ
HCCCCEEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCEECCHHHHHHHHHHHHHC
GHRRIAHIGGDPTLEHSMVRRRAFAQEAERLGAVVIEAAATDYSEQSGLRETNALLKTED
CCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHEEEEHHHCCCHHHCCCHHHHHHHCCCC
RPTAIVYDNEVLTLGGLAAVAEHRLQVPTDISVVSFEDSPACRVMSPGITALRRDPSVLG
CCEEEEECCCEEEECHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCCHHHHHCCCHHHH
RDAITMLMGILGGDDVEDVTEQPPELIVRGSTGQAQ
HHHHHHHHHHHCCCCHHHHHCCCCCEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA