Click here to switch to the map view.

The map label for this gene is lacF [H]

Identifier: 50842934

GI number: 50842934

Start: 1571072

End: 1572028

Strand: Direct

Name: lacF [H]

Synonym: PPA1455

Alternate gene names: 50842934

Gene position: 1571072-1572028 (Clockwise)

Preceding gene: 50842933

Following gene: 50842935

Centisome position: 61.36

GC content: 59.25

Gene sequence:

>957_bases
ATGAACTCCCACCGCTGGTACACACCGTGGTTGTTGGTGGCCCCGGCAGTCGTGTGGACCGTCGTGTTCGCCCTCTACCC
CTTCATCAACACGATCATCCTGTCCTTCACGAACACCCGACCCCTCGTCGGCGGGGAGTTCATCGGTTTCGCAAACTACG
CCGAACTGTGGCACGACGACATGTTCTGGAATGCCATGGTGACGACATTCATCTATGTCATCATCTGCATCCCGCTGTTG
ACGATCTTGCCATTGCTGCTGGCCCTCCTGGTCGAGAAGAAGATTCCGGGCATCTCGTTCTTCCGCACGGTGGCGTATTT
CCCCGTCATCGCATCGGTCGTCGTAGTCGCCCTCATCTGGAGCTGGATGTTCGATTCTCGCGGCCTTATTAACCAGTCCC
TGCAATGGATGGGCGCTACCAGCAGCCCCATCCCTTTCCTCGTCGATCGCTGGAAGCTGCTCGGCTGCGCGATCCTCCTG
ACGGTTTGGAAAGGCCTCGGTTACTACATGATCGTCTACCTCGCGGCGCTGGGAAACGTTGACCATGACCTCCACGAAGC
AGCTGCCCTCGACGGTGCGGGAACCTTCCAGAGATTCCGCAACGTCACCATTCCCGGTATCCGCCCCGCAATGGTCCTCA
TCATTGCCCTCATCGCGGTGTCCGGCATGAGGATCTTCTCCGAGCTGTACGTCCTCACCAACGGCACGGGTGGCCCAGGC
GGTGAGGACTCCAGTCTGGTCATGATCATTAAGCAAGTCGGAACCGGACTGTCCGGACAGCTCGGATACGCCTCAGCCCT
ATCAGTTGTGTTGTTCTTCCTCACCCTCATCCCATTGACGGTCATCGGGTGGGCCAACAACAAGGACCTCCGATCAGGAC
GTCGGACCAGACGAGGAGAAGGTCGCGCTCGCCGCGCCGCCGCTATCCAGAGCGCCACCAAGGAGACGATGGCATGA

Upstream 100 bases:

>100_bases
AACGACCACGTCACCAAGTGATCTTCCTGCTGGCTCCGCCCTGCTGCGGGACCAGCAGAATCGTCGTCCGCTCACCTACC
TCGCCCAAGGATTGCGCTCC

Downstream 100 bases:

>100_bases
GCACTGCACAGGCAACCCGTCCAGCCAAGCAGAAGCCGTGGCGCGGCCGACACGCCTTCGACCCAACTCGTCCCACCCTC
GCCGGGCTCATCGGGCGTTA

Product: transport system permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNSHRWYTPWLLVAPAVVWTVVFALYPFINTIILSFTNTRPLVGGEFIGFANYAELWHDDMFWNAMVTTFIYVIICIPLL
TILPLLLALLVEKKIPGISFFRTVAYFPVIASVVVVALIWSWMFDSRGLINQSLQWMGATSSPIPFLVDRWKLLGCAILL
TVWKGLGYYMIVYLAALGNVDHDLHEAAALDGAGTFQRFRNVTIPGIRPAMVLIIALIAVSGMRIFSELYVLTNGTGGPG
GEDSSLVMIIKQVGTGLSGQLGYASALSVVLFFLTLIPLTVIGWANNKDLRSGRRTRRGEGRARRAAAIQSATKETMA

Sequences:

>Translated_318_residues
MNSHRWYTPWLLVAPAVVWTVVFALYPFINTIILSFTNTRPLVGGEFIGFANYAELWHDDMFWNAMVTTFIYVIICIPLL
TILPLLLALLVEKKIPGISFFRTVAYFPVIASVVVVALIWSWMFDSRGLINQSLQWMGATSSPIPFLVDRWKLLGCAILL
TVWKGLGYYMIVYLAALGNVDHDLHEAAALDGAGTFQRFRNVTIPGIRPAMVLIIALIAVSGMRIFSELYVLTNGTGGPG
GEDSSLVMIIKQVGTGLSGQLGYASALSVVLFFLTLIPLTVIGWANNKDLRSGRRTRRGEGRARRAAAIQSATKETMA
>Mature_318_residues
MNSHRWYTPWLLVAPAVVWTVVFALYPFINTIILSFTNTRPLVGGEFIGFANYAELWHDDMFWNAMVTTFIYVIICIPLL
TILPLLLALLVEKKIPGISFFRTVAYFPVIASVVVVALIWSWMFDSRGLINQSLQWMGATSSPIPFLVDRWKLLGCAILL
TVWKGLGYYMIVYLAALGNVDHDLHEAAALDGAGTFQRFRNVTIPGIRPAMVLIIALIAVSGMRIFSELYVLTNGTGGPG
GEDSSLVMIIKQVGTGLSGQLGYASALSVVLFFLTLIPLTVIGWANNKDLRSGRRTRRGEGRARRAAAIQSATKETMA

Specific function: Part of the binding-protein-dependent transport system for lactose. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG1175

COG function: function code G; ABC-type sugar transport systems, permease components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789861, Length=287, Percent_Identity=30.6620209059233, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1787570, Length=262, Percent_Identity=25.9541984732824, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1790465, Length=241, Percent_Identity=28.6307053941909, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 35106; Mature: 35106

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSHRWYTPWLLVAPAVVWTVVFALYPFINTIILSFTNTRPLVGGEFIGFANYAELWHDD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MFWNAMVTTFIYVIICIPLLTILPLLLALLVEKKIPGISFFRTVAYFPVIASVVVVALIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SWMFDSRGLINQSLQWMGATSSPIPFLVDRWKLLGCAILLTVWKGLGYYMIVYLAALGNV
HHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHDLHEAAALDGAGTFQRFRNVTIPGIRPAMVLIIALIAVSGMRIFSELYVLTNGTGGPG
CHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GEDSSLVMIIKQVGTGLSGQLGYASALSVVLFFLTLIPLTVIGWANNKDLRSGRRTRRGE
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GRARRAAAIQSATKETMA
CHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNSHRWYTPWLLVAPAVVWTVVFALYPFINTIILSFTNTRPLVGGEFIGFANYAELWHDD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
MFWNAMVTTFIYVIICIPLLTILPLLLALLVEKKIPGISFFRTVAYFPVIASVVVVALIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
SWMFDSRGLINQSLQWMGATSSPIPFLVDRWKLLGCAILLTVWKGLGYYMIVYLAALGNV
HHHHCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DHDLHEAAALDGAGTFQRFRNVTIPGIRPAMVLIIALIAVSGMRIFSELYVLTNGTGGPG
CHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
GEDSSLVMIIKQVGTGLSGQLGYASALSVVLFFLTLIPLTVIGWANNKDLRSGRRTRRGE
CCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCC
GRARRAAAIQSATKETMA
CHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1630315 [H]