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The map label for this gene is yciL [C]

Identifier: 50842867

GI number: 50842867

Start: 1505705

End: 1506442

Strand: Reverse

Name: yciL [C]

Synonym: PPA1385

Alternate gene names: 50842867

Gene position: 1506442-1505705 (Counterclockwise)

Preceding gene: 50842868

Following gene: 50842866

Centisome position: 58.84

GC content: 59.89

Gene sequence:

>738_bases
ATGAGTAACGAGCAAGGCATCCGGCTGCAGAAGGTGCTGGCCCAGGCCGGTCTGGCATCCCGCCGTGCTGCTGAAGACAT
GATCGCGGCCGGAAGGGTTGAGGTCAATGGTGAACTCATCGTTGAGCAGGGGCGACGTGTTGACCCTGAGCGCGATGAGA
TCCGGGTCGATGGGTCCCGTATCCCGACTGCGCGGCGTCACGTCTACTACGTACTTAATAAGCCGCAGGGAGTTGTCTCT
ACTATGGACGATCCGGAAGGCCGCCCGTGCCTGTCCGACCTCGAAGGAGTGCCGCGCGGTATCCGTCTGTTTCACGTCGG
TCGTCTCGACACTGAGACCTCGGGCCTCATCTTGCTGACCAATGATGGCGAATTCGCTAACCGGATGACCCACCCGTCCT
ACAAGGTTCTCAAGACCTACTTGGCAGATGTTGAGGGCCGCGTGGATGACAAAGTCATTCACCGCCTTTCGAAAGGGCTG
ACCCTTGAAGACGGCCCTGTCAAGCCCGACGCCGTGCGCCTAGTACAGGCTGGCGAGGAGCATTCCTTGGTTGAGGTGAG
CCTTCATGAGGGCCGCAACCGGATTGTGCGTCGCATCATGGATTCGGTCGCTCATCCAGTGCGTCGACTGTCTCGCACCG
CGATCGGCCCGGTCCGATTGAAGCGGTTAGGGCGCGGAGATATGCGTGAACTTACCCATGAAGAACTGGGCCAGCTCCTC
GACCTCGTCGAACTGTGA

Upstream 100 bases:

>100_bases
GTGCCTCGGTGGCGATGGCCCTGGCCGATTTTGAGGAGAATGGTGAGGACTCTGCTCTCGCCCATCTCCTCACGACTGAC
TGAATACTTTAAGGACACAT

Downstream 100 bases:

>100_bases
GTTCTCGCGCATGGCTGGAATTGTCCTCAAGCATCGCTCGGGTAACCTCAAGCAGGTGAGTTCGAACAAGTATCGTCTGG
TCAACGCCGCGGCACTCGCT

Product: ribosomal large subunit pseudouridine synthase B

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MSNEQGIRLQKVLAQAGLASRRAAEDMIAAGRVEVNGELIVEQGRRVDPERDEIRVDGSRIPTARRHVYYVLNKPQGVVS
TMDDPEGRPCLSDLEGVPRGIRLFHVGRLDTETSGLILLTNDGEFANRMTHPSYKVLKTYLADVEGRVDDKVIHRLSKGL
TLEDGPVKPDAVRLVQAGEEHSLVEVSLHEGRNRIVRRIMDSVAHPVRRLSRTAIGPVRLKRLGRGDMRELTHEELGQLL
DLVEL

Sequences:

>Translated_245_residues
MSNEQGIRLQKVLAQAGLASRRAAEDMIAAGRVEVNGELIVEQGRRVDPERDEIRVDGSRIPTARRHVYYVLNKPQGVVS
TMDDPEGRPCLSDLEGVPRGIRLFHVGRLDTETSGLILLTNDGEFANRMTHPSYKVLKTYLADVEGRVDDKVIHRLSKGL
TLEDGPVKPDAVRLVQAGEEHSLVEVSLHEGRNRIVRRIMDSVAHPVRRLSRTAIGPVRLKRLGRGDMRELTHEELGQLL
DLVEL
>Mature_244_residues
SNEQGIRLQKVLAQAGLASRRAAEDMIAAGRVEVNGELIVEQGRRVDPERDEIRVDGSRIPTARRHVYYVLNKPQGVVST
MDDPEGRPCLSDLEGVPRGIRLFHVGRLDTETSGLILLTNDGEFANRMTHPSYKVLKTYLADVEGRVDDKVIHRLSKGLT
LEDGPVKPDAVRLVQAGEEHSLVEVSLHEGRNRIVRRIMDSVAHPVRRLSRTAIGPVRLKRLGRGDMRELTHEELGQLLD
LVEL

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=247, Percent_Identity=43.3198380566802, Blast_Score=157, Evalue=7e-40,
Organism=Escherichia coli, GI1788510, Length=239, Percent_Identity=35.1464435146443, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI1790453, Length=247, Percent_Identity=33.1983805668016, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=34.4632768361582, Blast_Score=97, Evalue=9e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27392; Mature: 27261

Theoretical pI: Translated: 7.84; Mature: 7.84

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNEQGIRLQKVLAQAGLASRRAAEDMIAAGRVEVNGELIVEQGRRVDPERDEIRVDGSR
CCCCCCHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCCHHEEECCCC
IPTARRHVYYVLNKPQGVVSTMDDPEGRPCLSDLEGVPRGIRLFHVGRLDTETSGLILLT
CCCCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCEEEEE
NDGEFANRMTHPSYKVLKTYLADVEGRVDDKVIHRLSKGLTLEDGPVKPDAVRLVQAGEE
CCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
HSLVEVSLHEGRNRIVRRIMDSVAHPVRRLSRTAIGPVRLKRLGRGDMRELTHEELGQLL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHH
DLVEL
HHHCC
>Mature Secondary Structure 
SNEQGIRLQKVLAQAGLASRRAAEDMIAAGRVEVNGELIVEQGRRVDPERDEIRVDGSR
CCCCCHHHHHHHHHHCHHHHHHHHHHHHCCEEEECCEEEEECCCCCCCCCHHEEECCCC
IPTARRHVYYVLNKPQGVVSTMDDPEGRPCLSDLEGVPRGIRLFHVGRLDTETSGLILLT
CCCCCCEEEEEEECCCCCEECCCCCCCCCHHHHHHCCCCCEEEEEECCCCCCCCCEEEEE
NDGEFANRMTHPSYKVLKTYLADVEGRVDDKVIHRLSKGLTLEDGPVKPDAVRLVQAGEE
CCCHHHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCC
HSLVEVSLHEGRNRIVRRIMDSVAHPVRRLSRTAIGPVRLKRLGRGDMRELTHEELGQLL
CCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHH
DLVEL
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]