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The map label for this gene is prfB

Identifier: 50842838

GI number: 50842838

Start: 1476990

End: 1478096

Strand: Reverse

Name: prfB

Synonym: PPA1354

Alternate gene names: 50842838

Gene position: 1478096-1476990 (Counterclockwise)

Preceding gene: 50842840

Following gene: 50842837

Centisome position: 57.73

GC content: 58.18

Gene sequence:

>1107_bases
GTGGCATTGGTAGACCTTCAGCAGACCGTTGAGGACCTCGACGCATCCCTGTCCTCGATTGAGAATGTTCTCGATCCGGA
GGCTAAGAAAGCGGAGATCGCGGACCTCGAGAGGCAGGTGGCCGTTCCCAATCTCTGGGACGACCAAGAGAATGCGCAGC
GGGTGACTTCTCGACTGTCGGCCCTGCAGGCTGAGATTGAACGTCTGGAGCGGTTGCGCGCGCGGATCGAGGATGTCCAC
GTGCTGTTGGAATTCGCGGCTGCTGATGACGACAAGGAATCCCTAGCTGAGGCGACGACCGAGACGGTTAAGCTTCGTGA
GGAGATTGACGCTCTCGAGGTGAGGACCTTGCTGTCGGGGCAATACGACGAGCGCGAGGCCGTCGTCACGATTCGTTCAG
AGGCTGGCGGTGTTGACGCTGCCGATTTTGCGGAGATGCTGCTACGCATGTACCTGCGTTGGTCCGAACGTCACGGCTAC
AAGGTTGAAATCCTCGACACTTCGTACGCTGAAGAGGCAGGCATTAAGTCGGCGACGTTCGTCGTTCATGCCCCCTTCGC
CTACGGCACTCTTTCGGTCGAGCAGGGCACCCACCGTCTGGTGCGCATTAGTCCCTTCGACAACCAGGGGCGCCGTCAGA
CATCCTTTGCCGGTGTCGAGGTGTTGCCCGTCGTTGAGGAGACCGACCACGTCGACATCCCAGAGTCCGATCTGCGCGTT
GACGTCTTCCATGCCTCGGGCCCTGGTGGCCAGGGTGTGAATACCACCGACTCTGCCGTTCGTCTGACACACCTTCCGAC
CGGCATCGTAGTGAGCTGTCAGAATGAGCGTTCCCAAATTCAGAACAAGGCCGCCGCCTTGCGAGTTCTTCAGGCCAAGC
TGCTTGAGAAAGCCCGTGAGGAACAGGAAGCCGAAATGAATTCATTGAAGTCGGAGGGCAACTCTTGGGGAGCCCAGATG
CGCTCCTACGTGCTGCATCCCTACCAAATGGTCAAGGATTTGCGTACGAATCATGAGGTCGGTAACACTGATGCCGTCTT
CGACGGAGATATCGACGACTTCATCGACGCTGGTATTCGTTGGCGTCGCAAGAACGAAACCGACTGA

Upstream 100 bases:

>100_bases
CAGGTGCACCTACTCTACCGATTTATCACGTGAAGCACACTTCCCGGGTGGAGAATCATCGCACCGGAGTTGTCGCCACG
TCCCTGTAGAGTTGCTCAAC

Downstream 100 bases:

>100_bases
CGGGGACAGACGTGATCACCTTCGATAGTGTCACAAAGACATATACCGGGCAGAGCCAAGCGGCTCTATCTGACGTCAAT
GTCGACATCGACAAGGGGGA

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MALVDLQQTVEDLDASLSSIENVLDPEAKKAEIADLERQVAVPNLWDDQENAQRVTSRLSALQAEIERLERLRARIEDVH
VLLEFAAADDDKESLAEATTETVKLREEIDALEVRTLLSGQYDEREAVVTIRSEAGGVDAADFAEMLLRMYLRWSERHGY
KVEILDTSYAEEAGIKSATFVVHAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSFAGVEVLPVVEETDHVDIPESDLRV
DVFHASGPGGQGVNTTDSAVRLTHLPTGIVVSCQNERSQIQNKAAALRVLQAKLLEKAREEQEAEMNSLKSEGNSWGAQM
RSYVLHPYQMVKDLRTNHEVGNTDAVFDGDIDDFIDAGIRWRRKNETD

Sequences:

>Translated_368_residues
MALVDLQQTVEDLDASLSSIENVLDPEAKKAEIADLERQVAVPNLWDDQENAQRVTSRLSALQAEIERLERLRARIEDVH
VLLEFAAADDDKESLAEATTETVKLREEIDALEVRTLLSGQYDEREAVVTIRSEAGGVDAADFAEMLLRMYLRWSERHGY
KVEILDTSYAEEAGIKSATFVVHAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSFAGVEVLPVVEETDHVDIPESDLRV
DVFHASGPGGQGVNTTDSAVRLTHLPTGIVVSCQNERSQIQNKAAALRVLQAKLLEKAREEQEAEMNSLKSEGNSWGAQM
RSYVLHPYQMVKDLRTNHEVGNTDAVFDGDIDDFIDAGIRWRRKNETD
>Mature_367_residues
ALVDLQQTVEDLDASLSSIENVLDPEAKKAEIADLERQVAVPNLWDDQENAQRVTSRLSALQAEIERLERLRARIEDVHV
LLEFAAADDDKESLAEATTETVKLREEIDALEVRTLLSGQYDEREAVVTIRSEAGGVDAADFAEMLLRMYLRWSERHGYK
VEILDTSYAEEAGIKSATFVVHAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSFAGVEVLPVVEETDHVDIPESDLRVD
VFHASGPGGQGVNTTDSAVRLTHLPTGIVVSCQNERSQIQNKAAALRVLQAKLLEKAREEQEAEMNSLKSEGNSWGAQMR
SYVLHPYQMVKDLRTNHEVGNTDAVFDGDIDDFIDAGIRWRRKNETD

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family

Homologues:

Organism=Homo sapiens, GI166795303, Length=280, Percent_Identity=34.2857142857143, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI34577120, Length=361, Percent_Identity=28.2548476454294, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI166795305, Length=183, Percent_Identity=34.9726775956284, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI2367172, Length=353, Percent_Identity=43.342776203966, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI1787462, Length=242, Percent_Identity=40.9090909090909, Blast_Score=169, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17542784, Length=279, Percent_Identity=30.4659498207885, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321295, Length=351, Percent_Identity=33.9031339031339, Blast_Score=137, Evalue=2e-33,
Organism=Drosophila melanogaster, GI19921226, Length=309, Percent_Identity=31.3915857605178, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF2_PROAC (Q6A808)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_056065.1
- HSSP:   Q9X183
- ProteinModelPortal:   Q6A808
- SMR:   Q6A808
- GeneID:   2931515
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1354
- NMPDR:   fig|267747.1.peg.1342
- HOGENOM:   HBG629764
- OMA:   TEMLLRM
- ProtClustDB:   PRK00578
- BioCyc:   PACN267747:PPA1354-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00094
- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374
- PANTHER:   PTHR11075:SF6
- SMART:   SM00937
- TIGRFAMs:   TIGR00020

Pfam domain/function: PF03462 PCRF; PF00472 RF-1

EC number: NA

Molecular weight: Translated: 41142; Mature: 41011

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVDLQQTVEDLDASLSSIENVLDPEAKKAEIADLERQVAVPNLWDDQENAQRVTSRLS
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ALQAEIERLERLRARIEDVHVLLEFAAADDDKESLAEATTETVKLREEIDALEVRTLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QYDEREAVVTIRSEAGGVDAADFAEMLLRMYLRWSERHGYKVEILDTSYAEEAGIKSATF
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEE
VVHAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSFAGVEVLPVVEETDHVDIPESDLRV
EEECCCEECEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEE
DVFHASGPGGQGVNTTDSAVRLTHLPTGIVVSCQNERSQIQNKAAALRVLQAKLLEKARE
EEEECCCCCCCCCCCCCHHEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EQEAEMNSLKSEGNSWGAQMRSYVLHPYQMVKDLRTNHEVGNTDAVFDGDIDDFIDAGIR
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEECCCHHHHHHCCCE
WRRKNETD
ECCCCCCC
>Mature Secondary Structure 
ALVDLQQTVEDLDASLSSIENVLDPEAKKAEIADLERQVAVPNLWDDQENAQRVTSRLS
CCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
ALQAEIERLERLRARIEDVHVLLEFAAADDDKESLAEATTETVKLREEIDALEVRTLLSG
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
QYDEREAVVTIRSEAGGVDAADFAEMLLRMYLRWSERHGYKVEILDTSYAEEAGIKSATF
CCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHCCCCEEEE
VVHAPFAYGTLSVEQGTHRLVRISPFDNQGRRQTSFAGVEVLPVVEETDHVDIPESDLRV
EEECCCEECEEEECCCCCEEEEECCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCEE
DVFHASGPGGQGVNTTDSAVRLTHLPTGIVVSCQNERSQIQNKAAALRVLQAKLLEKARE
EEEECCCCCCCCCCCCCHHEEEEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHH
EQEAEMNSLKSEGNSWGAQMRSYVLHPYQMVKDLRTNHEVGNTDAVFDGDIDDFIDAGIR
HHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCEECCCHHHHHHCCCE
WRRKNETD
ECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA