Click here to switch to the map view.

The map label for this gene is trpS2 [H]

Identifier: 50842807

GI number: 50842807

Start: 1443697

End: 1444788

Strand: Reverse

Name: trpS2 [H]

Synonym: PPA1327

Alternate gene names: 50842807

Gene position: 1444788-1443697 (Counterclockwise)

Preceding gene: 50842808

Following gene: 50842804

Centisome position: 56.43

GC content: 58.61

Gene sequence:

>1092_bases
ATGACCAGCGACGCCCCCGGGATTGGCTCGAACGCCGCCACTTTGGCGCGTTCCCAGGCTCGCAGTGACAAGGTCGAGGC
TGATTTGGCGGTCCATCCTGACAAGTGGCGCATTCTTACCGGGGACCGTCCTACTGGCAGCCTGCATATCGGTCACTACT
TCGGGTCGCTGGCGAATCGGGTACGCCTGCAGAACAAGGGCATTGAGTCTTTCCTTGTCGTCGCTGACTACCAGGTTATC
TATGACCGCGATGGGGTCGGTGACTTGCAGGCCAATGTTATGTCGAATGTCGCCGATTACCTGGCAATCGGCATTGACCC
AACGCTTACCACGATTTTCACCCACTCAGCGCTGCCAGCTCTCAATCAGCTGCTGTTGCCCTTTTTGTCTTTGGTGACTG
ACGCTGAGCTGCGTCGCAATCCGACTGTCAAGGACGAGTTGGCTACGTCAGAGCGTCCGATGTCCGGTCTCATGCTCACC
TTCCCGGTACATCAGGCCGCTGACATCCTCTTCTGTAAGGCGAACCTTGTTCCCGTCGGGCGTGATCAGCTTCCTCACAT
CGAGCAGACCCGTGTCATCGCGCGTCGTTTCGACGAGCGGTACGGGCGCGCCGAGGCCTCCAAGCCCGTCTTTCCCGAGC
CTGAAGCATTGTTGTCGGCGACTCCGCACGTTCTCGGCTTGGACGGCACCAAGATGAGCAAGTCGAAGGGTAACGCCATC
GAGCTCGGTATGACCGCAGACCAGACTGCAAAGCTCATCAAGAAGGCCAAGACCGACGCTGACCGTCACATCACGTGGGA
TCCGGAGAACCGCCCGGAGGTCTCCAACCTGCTGCTGCTCACCGCCCTGTGCGAACACGATGATCCGGCTACCATCGCTG
AGGAAATTGGTGATGGTGGCTCTGGCACTCTCAAGAAACGTCTCACTGCTTCCCTCAACGACTATTTCGCCCCCATTCGA
GAGCGTCGTGCCGAACTGGCAGCTGATGAGGATCACCTTCGTGAGGTTCTTCGTGATGGCAACGCGAAGGCCGCTGCGGT
AGCAGACCAGACGTTGGCGGAGGTGCGCGAAGCTATGAAAATGGTGTACTGA

Upstream 100 bases:

>100_bases
CCTCGTGGTGGCGTCGTTGCGTGCGGCACTGACAATGTGATGGCCGCCTTGCCTGGCGCGTCTCCTCGTCGCCGGGAGCG
GCGAGGAAGGATTAACAACG

Downstream 100 bases:

>100_bases
GGGAGTTGTTTCGTAGTCTTATCGCGCACCAGTGTGCCAGTAGCCTCATGGTGCGGGGCCTCGTCAAAAAGTGATGTCTT
CCTGGGCGTTGAGCTCGGGA

Product: tryptophanyl-tRNA synthetase

Products: NA

Alternate protein names: Tryptophan--tRNA ligase II; TrpRS II; Tryptophanyl-tRNA synthetase II [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTSDAPGIGSNAATLARSQARSDKVEADLAVHPDKWRILTGDRPTGSLHIGHYFGSLANRVRLQNKGIESFLVVADYQVI
YDRDGVGDLQANVMSNVADYLAIGIDPTLTTIFTHSALPALNQLLLPFLSLVTDAELRRNPTVKDELATSERPMSGLMLT
FPVHQAADILFCKANLVPVGRDQLPHIEQTRVIARRFDERYGRAEASKPVFPEPEALLSATPHVLGLDGTKMSKSKGNAI
ELGMTADQTAKLIKKAKTDADRHITWDPENRPEVSNLLLLTALCEHDDPATIAEEIGDGGSGTLKKRLTASLNDYFAPIR
ERRAELAADEDHLREVLRDGNAKAAAVADQTLAEVREAMKMVY

Sequences:

>Translated_363_residues
MTSDAPGIGSNAATLARSQARSDKVEADLAVHPDKWRILTGDRPTGSLHIGHYFGSLANRVRLQNKGIESFLVVADYQVI
YDRDGVGDLQANVMSNVADYLAIGIDPTLTTIFTHSALPALNQLLLPFLSLVTDAELRRNPTVKDELATSERPMSGLMLT
FPVHQAADILFCKANLVPVGRDQLPHIEQTRVIARRFDERYGRAEASKPVFPEPEALLSATPHVLGLDGTKMSKSKGNAI
ELGMTADQTAKLIKKAKTDADRHITWDPENRPEVSNLLLLTALCEHDDPATIAEEIGDGGSGTLKKRLTASLNDYFAPIR
ERRAELAADEDHLREVLRDGNAKAAAVADQTLAEVREAMKMVY
>Mature_362_residues
TSDAPGIGSNAATLARSQARSDKVEADLAVHPDKWRILTGDRPTGSLHIGHYFGSLANRVRLQNKGIESFLVVADYQVIY
DRDGVGDLQANVMSNVADYLAIGIDPTLTTIFTHSALPALNQLLLPFLSLVTDAELRRNPTVKDELATSERPMSGLMLTF
PVHQAADILFCKANLVPVGRDQLPHIEQTRVIARRFDERYGRAEASKPVFPEPEALLSATPHVLGLDGTKMSKSKGNAIE
LGMTADQTAKLIKKAKTDADRHITWDPENRPEVSNLLLLTALCEHDDPATIAEEIGDGGSGTLKKRLTASLNDYFAPIRE
RRAELAADEDHLREVLRDGNAKAAAVADQTLAEVREAMKMVY

Specific function: Catalyzes the formation of 5'adenyl-Trp and tRNA(Trp) but with 5-fold less activity than trpRS. Increases the solubility of the nitric oxide synthase oxygenase (nos), as well as its affinity for substrate L-arginine and its nitric-oxide synthase activity.

COG id: COG0180

COG function: function code J; Tryptophanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI7710154, Length=328, Percent_Identity=29.8780487804878, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI41352700, Length=183, Percent_Identity=32.2404371584699, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1789786, Length=334, Percent_Identity=33.2335329341317, Blast_Score=151, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI71982800, Length=348, Percent_Identity=31.8965517241379, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI71982793, Length=351, Percent_Identity=31.6239316239316, Blast_Score=127, Evalue=7e-30,
Organism=Saccharomyces cerevisiae, GI6320474, Length=349, Percent_Identity=28.6532951289398, Blast_Score=107, Evalue=4e-24,
Organism=Drosophila melanogaster, GI24666151, Length=339, Percent_Identity=31.5634218289086, Blast_Score=142, Evalue=4e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002306 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.2 [H]

Molecular weight: Translated: 39677; Mature: 39546

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSDAPGIGSNAATLARSQARSDKVEADLAVHPDKWRILTGDRPTGSLHIGHYFGSLANR
CCCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCEEHHHHHHHHHHH
VRLQNKGIESFLVVADYQVIYDRDGVGDLQANVMSNVADYLAIGIDPTLTTIFTHSALPA
HHHHCCCCCEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LNQLLLPFLSLVTDAELRRNPTVKDELATSERPMSGLMLTFPVHQAADILFCKANLVPVG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEECHHHHCCEEEEECCCCCCC
RDQLPHIEQTRVIARRFDERYGRAEASKPVFPEPEALLSATPHVLGLDGTKMSKSKGNAI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCEE
ELGMTADQTAKLIKKAKTDADRHITWDPENRPEVSNLLLLTALCEHDDPATIAEEIGDGG
EECCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCC
SGTLKKRLTASLNDYFAPIRERRAELAADEDHLREVLRDGNAKAAAVADQTLAEVREAMK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
MVY
HCC
>Mature Secondary Structure 
TSDAPGIGSNAATLARSQARSDKVEADLAVHPDKWRILTGDRPTGSLHIGHYFGSLANR
CCCCCCCCCCHHHHHHHHHHCCCCCCCEEECCCCEEEEECCCCCCCEEHHHHHHHHHHH
VRLQNKGIESFLVVADYQVIYDRDGVGDLQANVMSNVADYLAIGIDPTLTTIFTHSALPA
HHHHCCCCCEEEEEECEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
LNQLLLPFLSLVTDAELRRNPTVKDELATSERPMSGLMLTFPVHQAADILFCKANLVPVG
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCEEEEECHHHHCCEEEEECCCCCCC
RDQLPHIEQTRVIARRFDERYGRAEASKPVFPEPEALLSATPHVLGLDGTKMSKSKGNAI
CCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEECCCCCCCCCCCCEE
ELGMTADQTAKLIKKAKTDADRHITWDPENRPEVSNLLLLTALCEHDDPATIAEEIGDGG
EECCCHHHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHHHCCCCCHHHHHHHCCCC
SGTLKKRLTASLNDYFAPIRERRAELAADEDHLREVLRDGNAKAAAVADQTLAEVREAMK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHH
MVY
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10567266 [H]