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The map label for this gene is rpoE [H]

Identifier: 50842745

GI number: 50842745

Start: 1373291

End: 1373968

Strand: Reverse

Name: rpoE [H]

Synonym: PPA1266

Alternate gene names: 50842745

Gene position: 1373968-1373291 (Counterclockwise)

Preceding gene: 50842749

Following gene: 50842744

Centisome position: 53.67

GC content: 58.55

Gene sequence:

>678_bases
ATGGATCGTCGCGTGGATGCGTCGGGCACCGTGGACGTCGCTGAAGAGACGAAAACCCAGAGGGCGCAACGTTTCGAGCG
TGACGCCCTCGAATACCTTGACCAGCTCTACGGCGCCGCGCTGCGCATGACCCGCAACCCGGCTGACGCCGAAGATGTTG
TCCAAGAGGCCTATGCCAAAGCTTTTTCTTCGTTCCACCAGTTCCGACCTGGTACGAACCTCAAGGCTTGGCTGTATCGC
ATTCTGACGAATACCTACATCAACTCGTACCGCAAGGCTCAGCGTCGCCCCCAGACATCAGATGACCAGGACGTCGAGGA
TTGGCAGATCGCTAAGGCTGCGTCCCATGACAGCAACGGTTTGCGGTCTGCCGAGTTGGAGGCCCTAGATGGGCTGCCTG
ACCAGCAGATTCTAGACGCTCTGGACGGGTTGTCGGATGAGTTCCGTCAGGTCGTTTTGCTGGCTGATGTCGAGGGTTTC
GCTTACAAGGAAATCGCTGAAATCATGGGCACCCCAATGGGGACCGTGATGAGTCGGCTGAATCGTGCTCGCAAGCAGCT
GCGTAAGCAGTTGGTCGACGTCGCAGGTGCTCGAGGGATCGGTCCTCGCGCCGGGCAAGTAGATGAAGAGGATCTTGGTC
GTTCTGCGATGGCCCGTACAGCAGGAAGGGAGGGATGA

Upstream 100 bases:

>100_bases
GCTTATCGCGCCCCGTACGTTGCGGAGGCCTCGCTCTTTCGAGGGCCAACGCTGTGCACTAAGCTGGCACACGATGATCA
CACGTGAATCCGAGGACAGG

Downstream 100 bases:

>100_bases
CGATGACCCAGTCCGCTGGTAACAAGGAATCCTGTTATTGGGTCATTGACCACCTCCAGGAATTCCTCCATGGAGAAATG
AGTGAGCACGATGCTGATGC

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDRRVDASGTVDVAEETKTQRAQRFERDALEYLDQLYGAALRMTRNPADAEDVVQEAYAKAFSSFHQFRPGTNLKAWLYR
ILTNTYINSYRKAQRRPQTSDDQDVEDWQIAKAASHDSNGLRSAELEALDGLPDQQILDALDGLSDEFRQVVLLADVEGF
AYKEIAEIMGTPMGTVMSRLNRARKQLRKQLVDVAGARGIGPRAGQVDEEDLGRSAMARTAGREG

Sequences:

>Translated_225_residues
MDRRVDASGTVDVAEETKTQRAQRFERDALEYLDQLYGAALRMTRNPADAEDVVQEAYAKAFSSFHQFRPGTNLKAWLYR
ILTNTYINSYRKAQRRPQTSDDQDVEDWQIAKAASHDSNGLRSAELEALDGLPDQQILDALDGLSDEFRQVVLLADVEGF
AYKEIAEIMGTPMGTVMSRLNRARKQLRKQLVDVAGARGIGPRAGQVDEEDLGRSAMARTAGREG
>Mature_225_residues
MDRRVDASGTVDVAEETKTQRAQRFERDALEYLDQLYGAALRMTRNPADAEDVVQEAYAKAFSSFHQFRPGTNLKAWLYR
ILTNTYINSYRKAQRRPQTSDDQDVEDWQIAKAASHDSNGLRSAELEALDGLPDQQILDALDGLSDEFRQVVLLADVEGF
AYKEIAEIMGTPMGTVMSRLNRARKQLRKQLVDVAGARGIGPRAGQVDEEDLGRSAMARTAGREG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=144, Percent_Identity=35.4166666666667, Blast_Score=86, Evalue=3e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014293
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 25155; Mature: 25155

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRRVDASGTVDVAEETKTQRAQRFERDALEYLDQLYGAALRMTRNPADAEDVVQEAYAK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AFSSFHQFRPGTNLKAWLYRILTNTYINSYRKAQRRPQTSDDQDVEDWQIAKAASHDSNG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LRSAELEALDGLPDQQILDALDGLSDEFRQVVLLADVEGFAYKEIAEIMGTPMGTVMSRL
CHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
NRARKQLRKQLVDVAGARGIGPRAGQVDEEDLGRSAMARTAGREG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDRRVDASGTVDVAEETKTQRAQRFERDALEYLDQLYGAALRMTRNPADAEDVVQEAYAK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
AFSSFHQFRPGTNLKAWLYRILTNTYINSYRKAQRRPQTSDDQDVEDWQIAKAASHDSNG
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LRSAELEALDGLPDQQILDALDGLSDEFRQVVLLADVEGFAYKEIAEIMGTPMGTVMSRL
CHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHH
NRARKQLRKQLVDVAGARGIGPRAGQVDEEDLGRSAMARTAGREG
HHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]