Click here to switch to the map view.

The map label for this gene is gltB [H]

Identifier: 50842614

GI number: 50842614

Start: 1234587

End: 1236059

Strand: Direct

Name: gltB [H]

Synonym: PPA1135

Alternate gene names: 50842614

Gene position: 1234587-1236059 (Clockwise)

Preceding gene: 50842613

Following gene: 50842615

Centisome position: 48.22

GC content: 59.2

Gene sequence:

>1473_bases
ATGGCTGATCTGCACGGCTTTATGAATGTGACTCGACAGGAGGCGGAGCGTCGTCCAGTCGAAGAGCGCGTCCAGGACTG
GAAAGAGGTCTACCCAGGCGGACCTGGTCACGCTCTGCTGCCCATCATTACAGACCAAGCGAGCCGGTGCATGGACTGTG
GTGTGCCCTTCTGCCATACCGGCTGTCCGTTGGGTAATCTCATCCCTGACTGGAACGACCTCATCTGGCGAGACGAATGG
CGCGAGGCTCTCAGCTGTTTGCAGACCACCAACAACTTTCCAGAGTTCACCGGTCGATTGTGCCCCGCTCCATGCGAGAC
GGCGTGTGTCGAAGGGCTTAACCGCGCCCCGGTCACGATTAGGAACGTTGAGGTTGCCATCATTGATAAGGCCTGGGAAG
ACCGCCGCGTCATTCCGGTGGTGCCTGAGTGGCATTCACTCAAGACGGCTGCAGTGGTCGGATCTGGCCCCGCAGGACTG
GCAACCGCCCAGCAGCTCACCCGTGCGGGGCATACGGTCGTCGTCTACGAGCGTGACGACGCCATCGGCGGGTTGCTGCG
TTATGGCATCCCGGATTTTAAGATGGAAAAATCGGTTCTCGACCGCCGGATTAAGCAGATGGTGTTGGAGGGTACCGTCT
TCAAGACGAATACTGAGATCGGCGTCGATATCACTGGCGAGCAGTTACGCGAGCGGTTTGACGCCGTCGTCTTGGCTACC
GGTGCCACCGTTCCACGCATGCTGGATGCTCCAGGTATTGAGCTCGACGGGGTCTGTTATGCCATGGAGTACCTCACTCA
GCAAAACCGCGTCGTGGCGGGGGCAGAGGTGCTTGATCAGATCCACGCTGACGGTAAGCATGTTGTCATCATCGGTGGCG
GAGATACTGCCACCGACTGTGTAGGCACCGCAGTGCGTCAGGGCGCGGCCTCAATTACCCAGCTGCAGTACAACCCGGAG
CCGCCCAAGGACCGATCGGCTAACTATCCCTGGCCCACTTATCCGCTCATTTTACGGGTCTCCAGCTCCAATGAAGAGGG
CGGACAGCGGGTGTACTCGACCAACACCGTCGAGATCGTCAGTAATGACCGGGGCCACGTCAAGGCAGTTCGGGTGGTCT
CAGGCCATCGCGATGAGAACCGGGTGTTTATTGCGGACGAGGGCAGCGAACATGATCTGCCGGCCGACCTAGTGATCTTT
GCGATGGGATTCGCTCATCCGGAGTATGACGGTGTTGTTGCCGAGCTGGGGCTGGAACTTGACGGACGGGGCAATATTAT
CAGAAATGATGCGTATGAGACTAATGTGCCGGGGGTTTTCGCCTGTGGTGACGCTGGCCGCGGGCAGTCCCTCGTGGTGT
GGGCCATCGCGGAGGGACGCGCAGCTGCTCACGGTGTTGATGCGTATCTCAAAGGATCACCGTCGGTGCTACCGAAGCCG
GTAAAACCGTCGGATCGTCAGATGACGGTGTAG

Upstream 100 bases:

>100_bases
AAAGTGCTGCCGCGCGACTACGCCACGATGATGAAGGCCATGGTCGACGCTGAGGAGCACGGCCTGGACGAGAACGAGAC
GACGTTCATGGAGGTGTCTC

Downstream 100 bases:

>100_bases
TCCCGAGGTGATCTCTTAGGCTCGACAGGAGGCGCCGTGCAGGAGGAAACTGCTGGGGTGAACGATAATGAGGAGGATCT
CGACTCGACGATTCGCCACG

Product: glutamate synthase small subunit

Products: NA

Alternate protein names: NADPH-GOGAT [H]

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MADLHGFMNVTRQEAERRPVEERVQDWKEVYPGGPGHALLPIITDQASRCMDCGVPFCHTGCPLGNLIPDWNDLIWRDEW
REALSCLQTTNNFPEFTGRLCPAPCETACVEGLNRAPVTIRNVEVAIIDKAWEDRRVIPVVPEWHSLKTAAVVGSGPAGL
ATAQQLTRAGHTVVVYERDDAIGGLLRYGIPDFKMEKSVLDRRIKQMVLEGTVFKTNTEIGVDITGEQLRERFDAVVLAT
GATVPRMLDAPGIELDGVCYAMEYLTQQNRVVAGAEVLDQIHADGKHVVIIGGGDTATDCVGTAVRQGAASITQLQYNPE
PPKDRSANYPWPTYPLILRVSSSNEEGGQRVYSTNTVEIVSNDRGHVKAVRVVSGHRDENRVFIADEGSEHDLPADLVIF
AMGFAHPEYDGVVAELGLELDGRGNIIRNDAYETNVPGVFACGDAGRGQSLVVWAIAEGRAAAHGVDAYLKGSPSVLPKP
VKPSDRQMTV

Sequences:

>Translated_490_residues
MADLHGFMNVTRQEAERRPVEERVQDWKEVYPGGPGHALLPIITDQASRCMDCGVPFCHTGCPLGNLIPDWNDLIWRDEW
REALSCLQTTNNFPEFTGRLCPAPCETACVEGLNRAPVTIRNVEVAIIDKAWEDRRVIPVVPEWHSLKTAAVVGSGPAGL
ATAQQLTRAGHTVVVYERDDAIGGLLRYGIPDFKMEKSVLDRRIKQMVLEGTVFKTNTEIGVDITGEQLRERFDAVVLAT
GATVPRMLDAPGIELDGVCYAMEYLTQQNRVVAGAEVLDQIHADGKHVVIIGGGDTATDCVGTAVRQGAASITQLQYNPE
PPKDRSANYPWPTYPLILRVSSSNEEGGQRVYSTNTVEIVSNDRGHVKAVRVVSGHRDENRVFIADEGSEHDLPADLVIF
AMGFAHPEYDGVVAELGLELDGRGNIIRNDAYETNVPGVFACGDAGRGQSLVVWAIAEGRAAAHGVDAYLKGSPSVLPKP
VKPSDRQMTV
>Mature_489_residues
ADLHGFMNVTRQEAERRPVEERVQDWKEVYPGGPGHALLPIITDQASRCMDCGVPFCHTGCPLGNLIPDWNDLIWRDEWR
EALSCLQTTNNFPEFTGRLCPAPCETACVEGLNRAPVTIRNVEVAIIDKAWEDRRVIPVVPEWHSLKTAAVVGSGPAGLA
TAQQLTRAGHTVVVYERDDAIGGLLRYGIPDFKMEKSVLDRRIKQMVLEGTVFKTNTEIGVDITGEQLRERFDAVVLATG
ATVPRMLDAPGIELDGVCYAMEYLTQQNRVVAGAEVLDQIHADGKHVVIIGGGDTATDCVGTAVRQGAASITQLQYNPEP
PKDRSANYPWPTYPLILRVSSSNEEGGQRVYSTNTVEIVSNDRGHVKAVRVVSGHRDENRVFIADEGSEHDLPADLVIFA
MGFAHPEYDGVVAELGLELDGRGNIIRNDAYETNVPGVFACGDAGRGQSLVVWAIAEGRAAAHGVDAYLKGSPSVLPKPV
KPSDRQMTV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamate synthase family [H]

Homologues:

Organism=Homo sapiens, GI111118981, Length=150, Percent_Identity=38, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI111118983, Length=150, Percent_Identity=38, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1789606, Length=485, Percent_Identity=36.9072164948454, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI1788811, Length=478, Percent_Identity=36.8200836820084, Blast_Score=242, Evalue=3e-65,
Organism=Escherichia coli, GI87082180, Length=482, Percent_Identity=34.8547717842324, Blast_Score=204, Evalue=9e-54,
Organism=Escherichia coli, GI1788468, Length=441, Percent_Identity=28.5714285714286, Blast_Score=146, Evalue=3e-36,
Organism=Escherichia coli, GI1789243, Length=432, Percent_Identity=26.8518518518519, Blast_Score=107, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17570289, Length=493, Percent_Identity=44.6247464503043, Blast_Score=413, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI17543860, Length=154, Percent_Identity=35.7142857142857, Blast_Score=72, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=491, Percent_Identity=42.1588594704684, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI28574881, Length=489, Percent_Identity=50.3067484662577, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24665539, Length=489, Percent_Identity=50.3067484662577, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24665547, Length=490, Percent_Identity=50.2040816326531, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24665543, Length=490, Percent_Identity=50.2040816326531, Blast_Score=452, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24640763, Length=462, Percent_Identity=23.5930735930736, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI18858217, Length=462, Percent_Identity=23.5930735930736, Blast_Score=94, Evalue=3e-19,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006005
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 53409; Mature: 53278

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADLHGFMNVTRQEAERRPVEERVQDWKEVYPGGPGHALLPIITDQASRCMDCGVPFCHT
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCCHHCC
GCPLGNLIPDWNDLIWRDEWREALSCLQTTNNFPEFTGRLCPAPCETACVEGLNRAPVTI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHCCCCCCCEEE
RNVEVAIIDKAWEDRRVIPVVPEWHSLKTAAVVGSGPAGLATAQQLTRAGHTVVVYERDD
EEEEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCC
AIGGLLRYGIPDFKMEKSVLDRRIKQMVLEGTVFKTNTEIGVDITGEQLRERFDAVVLAT
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECHHHHHHHHCEEEEEC
GATVPRMLDAPGIELDGVCYAMEYLTQQNRVVAGAEVLDQIHADGKHVVIIGGGDTATDC
CCCCCHHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEECCCCHHHH
VGTAVRQGAASITQLQYNPEPPKDRSANYPWPTYPLILRVSSSNEEGGQRVYSTNTVEIV
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCEEEEE
SNDRGHVKAVRVVSGHRDENRVFIADEGSEHDLPADLVIFAMGFAHPEYDGVVAELGLEL
ECCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCEEE
DGRGNIIRNDAYETNVPGVFACGDAGRGQSLVVWAIAEGRAAAHGVDAYLKGSPSVLPKP
CCCCCEEECCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCHHCCCCCEECCCCCCCCCC
VKPSDRQMTV
CCCCCCCCCC
>Mature Secondary Structure 
ADLHGFMNVTRQEAERRPVEERVQDWKEVYPGGPGHALLPIITDQASRCMDCGVPFCHT
CCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEEEEEECCHHHHHHHCCCCHHCC
GCPLGNLIPDWNDLIWRDEWREALSCLQTTNNFPEFTGRLCPAPCETACVEGLNRAPVTI
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHHHCCCCCCCEEE
RNVEVAIIDKAWEDRRVIPVVPEWHSLKTAAVVGSGPAGLATAQQLTRAGHTVVVYERDD
EEEEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCCEEEEEECCC
AIGGLLRYGIPDFKMEKSVLDRRIKQMVLEGTVFKTNTEIGVDITGEQLRERFDAVVLAT
CHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECHHHHHHHHCEEEEEC
GATVPRMLDAPGIELDGVCYAMEYLTQQNRVVAGAEVLDQIHADGKHVVIIGGGDTATDC
CCCCCHHHCCCCCCCCHHHHHHHHHHHCCCEEEHHHHHHHHHCCCCEEEEEECCCCHHHH
VGTAVRQGAASITQLQYNPEPPKDRSANYPWPTYPLILRVSSSNEEGGQRVYSTNTVEIV
HHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCEEEECCEEEEE
SNDRGHVKAVRVVSGHRDENRVFIADEGSEHDLPADLVIFAMGFAHPEYDGVVAELGLEL
ECCCCCEEEEEEEECCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHCEEE
DGRGNIIRNDAYETNVPGVFACGDAGRGQSLVVWAIAEGRAAAHGVDAYLKGSPSVLPKP
CCCCCEEECCCCCCCCCEEEEECCCCCCCEEEEEEEECCCCHHCCCCCEECCCCCCCCCC
VKPSDRQMTV
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377; 11029411 [H]