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The map label for this gene is glgB

Identifier: 50842590

GI number: 50842590

Start: 1206207

End: 1208141

Strand: Reverse

Name: glgB

Synonym: PPA1111

Alternate gene names: 50842590

Gene position: 1208141-1206207 (Counterclockwise)

Preceding gene: 50842591

Following gene: 50842589

Centisome position: 47.19

GC content: 59.02

Gene sequence:

>1935_bases
ATGGCTCACGATGAGTTCGGAGGACTCACCGGTTGGGACCTCGAGGGGTTCCACTCTGGTGGGGACACGGAGGTGTGGAA
GAGGCTCGGGTCCCATGTCGTCACTATCGACGACGATGAGCGCGGTCCCATCACCGGCACCCGCTTCGCTGTGTGGGCCC
CAAATGCCCAGGCGGTTGAGGTCATCTCTGACTTCAACTGGTGGACTGGTGACCGTATGCGCCTCATCCCGGGGTCCGGA
GTCTGGGGCACCTTCGTCGAAGGAGTCGACGAGGGAACCCTCTATAAGTTCCGCATTCAGGACCAGTGGGGTACCTGGCA
CGAAAAGGTTGACCCAATGGCCCGTTATTCAGAGCAAGCTCCTCAGAATGCTTCCATCGTCACCGAAACCCACTACGAAT
GGAATGACGATGAGTGGATCGCCCGCCGCGAGGCCTCCCGCGCTCACGCCGAACCGATGAGCGTCTACGAGGTCCATTTG
GGCGGATGGCGTCACGGGCTGAGCTACCGAGAGTTGGCTGACCAGCTGGTGAGCTATGTCACTTGGCAGGGCTATACCCA
TGTGGAGTTCATGCCATTGGCTGAACACCCATTCGCTCCCTCCTGGGGATACCAGGTCACCGGGTACTTCTCTCCCACCA
GCCGCTACGGATCTCCCGACGACCTGCGTTACCTCATCGATAAGCTACATCAGGCCGGCATCGGAGTCATCATGGATTGG
GTTCCCGGCCACTTTCCCAAGGACGACTGGGCCCTGGGGCGCTTCGACGGCACCGCGCTTTACGAACACGCCGATCCACG
CCAGGGCGAACACAAGGACTGGGGAACCTACATCTTCAACTACGGTCGTAACGAGGTGAAATCTTTCCTCGTCTCAAGCG
CGCTGTACTGGATTAGCGAATTCCACGCCGACGGACTGCGCGTTGACGCCGTCGCCTCGATGCTCTACTTGGACTACTCT
CGCGAAGAGGGCCAGTGGGTGCCCAACAAGTATGGCGGCCGGGAGAACCTAGAGGCCATTGACTTCTTGCGCTATGTCAA
CTCTCACTTGTATTCGCGCCACCCCGGCATCCTCATGATCGCTGAGGAGTCCACTAGTTTCCCCGGCGTCACCAAGCCGG
TCGACGACGGTGGGCTGGGGTTCGGGTTCAAGTGGAATATGGGCTGGATGAACGACTCGCTGCGATATCTGGAGCTCAAC
CCATTCCACCGCCAGTACCACCATGGCGAGATGACCTTTGCCATGGTCTACCAATACTCAGAGAACTTCATCTTGCCGAT
TAGCCACGACGAGGTCGTGCATGGCAAGGGATCCATGATCACCAAGATTCCCGGTGACGACTGGCAACAGTTCGCGTCCC
TGCGAGCCTTCTACTCCTACATGTGGTCTTTTCCCGGCAAACAGCTGGTGTTCATGGGTCAGGAATTTGGTCAGCGCCAC
GAGTTCGACGAGTCGGTAAGCCTTGAGTGGTTTGTCGCCGACCTGTGGGGACACGGCGGTCTCAAACGCCTCTTCCGCGA
CCTTAACAAGATTTACAAAGAAAACCCCGCATTGTGGCAGCTCGACAGCGATCCGCGCGGATTCGAGTGGATCAACGCCG
ACGATGCCGGTAACAACCTGTTCTCCTGGTTACGCCGTTCCGATGATGGATCGACGATTGCCTGCTTCACGAACTTCTCT
CCCAATCCGCAGACCGACTATCGTATCGACCTACCGATGGAAGGGGTGTGGACCGAGATCCTCAACACCGATTCCCTGGA
GTACGACGGCACCGGCGAGTTCGGCAATCTCGGCCAAATCGTAGCCGCCCCACTGCCAGCTCCCGATCGTCTCCGTGCGG
TTGCCACAGTGTGCGTTCCGCCGATGGGATCGGTGTGGCTACGTCACAATCCGTCAGCAACCGCTGCCCTGCCCGGCGAT
CCGGGCGTGCAATGA

Upstream 100 bases:

>100_bases
TCGAGCAATGGGGCAGGATTGACGATGCTCAAACTCACCCACGGATTCCCGTTGGATTATCGAACTGACGGCCACACCAA
CGACATGCCAGGAGGACGAC

Downstream 100 bases:

>100_bases
CCGTGCCACAGGAGGAATAATTCGTGGTCGATGACGAATCCGGTTCCGAGGATTTCAGAATCCAGGTCGAGGTGGTAGAT
GACTCCGGTCGGCTACTCCT

Product: glycogen branching enzyme

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE

Number of amino acids: Translated: 644; Mature: 643

Protein sequence:

>644_residues
MAHDEFGGLTGWDLEGFHSGGDTEVWKRLGSHVVTIDDDERGPITGTRFAVWAPNAQAVEVISDFNWWTGDRMRLIPGSG
VWGTFVEGVDEGTLYKFRIQDQWGTWHEKVDPMARYSEQAPQNASIVTETHYEWNDDEWIARREASRAHAEPMSVYEVHL
GGWRHGLSYRELADQLVSYVTWQGYTHVEFMPLAEHPFAPSWGYQVTGYFSPTSRYGSPDDLRYLIDKLHQAGIGVIMDW
VPGHFPKDDWALGRFDGTALYEHADPRQGEHKDWGTYIFNYGRNEVKSFLVSSALYWISEFHADGLRVDAVASMLYLDYS
REEGQWVPNKYGGRENLEAIDFLRYVNSHLYSRHPGILMIAEESTSFPGVTKPVDDGGLGFGFKWNMGWMNDSLRYLELN
PFHRQYHHGEMTFAMVYQYSENFILPISHDEVVHGKGSMITKIPGDDWQQFASLRAFYSYMWSFPGKQLVFMGQEFGQRH
EFDESVSLEWFVADLWGHGGLKRLFRDLNKIYKENPALWQLDSDPRGFEWINADDAGNNLFSWLRRSDDGSTIACFTNFS
PNPQTDYRIDLPMEGVWTEILNTDSLEYDGTGEFGNLGQIVAAPLPAPDRLRAVATVCVPPMGSVWLRHNPSATAALPGD
PGVQ

Sequences:

>Translated_644_residues
MAHDEFGGLTGWDLEGFHSGGDTEVWKRLGSHVVTIDDDERGPITGTRFAVWAPNAQAVEVISDFNWWTGDRMRLIPGSG
VWGTFVEGVDEGTLYKFRIQDQWGTWHEKVDPMARYSEQAPQNASIVTETHYEWNDDEWIARREASRAHAEPMSVYEVHL
GGWRHGLSYRELADQLVSYVTWQGYTHVEFMPLAEHPFAPSWGYQVTGYFSPTSRYGSPDDLRYLIDKLHQAGIGVIMDW
VPGHFPKDDWALGRFDGTALYEHADPRQGEHKDWGTYIFNYGRNEVKSFLVSSALYWISEFHADGLRVDAVASMLYLDYS
REEGQWVPNKYGGRENLEAIDFLRYVNSHLYSRHPGILMIAEESTSFPGVTKPVDDGGLGFGFKWNMGWMNDSLRYLELN
PFHRQYHHGEMTFAMVYQYSENFILPISHDEVVHGKGSMITKIPGDDWQQFASLRAFYSYMWSFPGKQLVFMGQEFGQRH
EFDESVSLEWFVADLWGHGGLKRLFRDLNKIYKENPALWQLDSDPRGFEWINADDAGNNLFSWLRRSDDGSTIACFTNFS
PNPQTDYRIDLPMEGVWTEILNTDSLEYDGTGEFGNLGQIVAAPLPAPDRLRAVATVCVPPMGSVWLRHNPSATAALPGD
PGVQ
>Mature_643_residues
AHDEFGGLTGWDLEGFHSGGDTEVWKRLGSHVVTIDDDERGPITGTRFAVWAPNAQAVEVISDFNWWTGDRMRLIPGSGV
WGTFVEGVDEGTLYKFRIQDQWGTWHEKVDPMARYSEQAPQNASIVTETHYEWNDDEWIARREASRAHAEPMSVYEVHLG
GWRHGLSYRELADQLVSYVTWQGYTHVEFMPLAEHPFAPSWGYQVTGYFSPTSRYGSPDDLRYLIDKLHQAGIGVIMDWV
PGHFPKDDWALGRFDGTALYEHADPRQGEHKDWGTYIFNYGRNEVKSFLVSSALYWISEFHADGLRVDAVASMLYLDYSR
EEGQWVPNKYGGRENLEAIDFLRYVNSHLYSRHPGILMIAEESTSFPGVTKPVDDGGLGFGFKWNMGWMNDSLRYLELNP
FHRQYHHGEMTFAMVYQYSENFILPISHDEVVHGKGSMITKIPGDDWQQFASLRAFYSYMWSFPGKQLVFMGQEFGQRHE
FDESVSLEWFVADLWGHGGLKRLFRDLNKIYKENPALWQLDSDPRGFEWINADDAGNNLFSWLRRSDDGSTIACFTNFSP
NPQTDYRIDLPMEGVWTEILNTDSLEYDGTGEFGNLGQIVAAPLPAPDRLRAVATVCVPPMGSVWLRHNPSATAALPGDP
GVQ

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family

Homologues:

Organism=Homo sapiens, GI189458812, Length=641, Percent_Identity=25.585023400936, Blast_Score=160, Evalue=3e-39,
Organism=Escherichia coli, GI1789839, Length=613, Percent_Identity=50.8972267536705, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17554896, Length=360, Percent_Identity=29.7222222222222, Blast_Score=176, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI32564391, Length=291, Percent_Identity=31.6151202749141, Blast_Score=166, Evalue=5e-41,
Organism=Saccharomyces cerevisiae, GI6320826, Length=646, Percent_Identity=22.9102167182663, Blast_Score=141, Evalue=3e-34,
Organism=Drosophila melanogaster, GI28573410, Length=357, Percent_Identity=32.2128851540616, Blast_Score=166, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GLGB_PROAC (Q6A8Q7)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055817.1
- ProteinModelPortal:   Q6A8Q7
- GeneID:   2930991
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA1111
- NMPDR:   fig|267747.1.peg.1094
- HOGENOM:   HBG287139
- OMA:   EWIARRE
- ProtClustDB:   PRK12313
- BioCyc:   PACN267747:PPA1111-MONOMER
- BRENDA:   2.4.1.18
- HAMAP:   MF_00685
- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756
- Gene3D:   G3DSA:2.60.40.1180
- Gene3D:   G3DSA:3.20.20.80
- Gene3D:   G3DSA:2.60.40.10
- TIGRFAMs:   TIGR01515

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48; SSF51445 Glyco_hydro_cat; SSF81296 Ig_E-set

EC number: =2.4.1.18

Molecular weight: Translated: 73563; Mature: 73432

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: ACT_SITE 204-204 ACT_SITE 239-239 ACT_SITE 244-244 ACT_SITE 307-307 ACT_SITE 309-309 ACT_SITE 362-362 ACT_SITE 429-429 ACT_SITE 430-430

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHDEFGGLTGWDLEGFHSGGDTEVWKRLGSHVVTIDDDERGPITGTRFAVWAPNAQAVE
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCHHH
VISDFNWWTGDRMRLIPGSGVWGTFVEGVDEGTLYKFRIQDQWGTWHEKVDPMARYSEQA
HHHCCCCCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHCCHHHHHHHCC
PQNASIVTETHYEWNDDEWIARREASRAHAEPMSVYEVHLGGWRHGLSYRELADQLVSYV
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHCCCCHHHHHHHHHHHH
TWQGYTHVEFMPLAEHPFAPSWGYQVTGYFSPTSRYGSPDDLRYLIDKLHQAGIGVIMDW
HCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
VPGHFPKDDWALGRFDGTALYEHADPRQGEHKDWGTYIFNYGRNEVKSFLVSSALYWISE
CCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHH
FHADGLRVDAVASMLYLDYSREEGQWVPNKYGGRENLEAIDFLRYVNSHLYSRHPGILMI
HCCCCEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
AEESTSFPGVTKPVDDGGLGFGFKWNMGWMNDSLRYLELNPFHRQYHHGEMTFAMVYQYS
EECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECHHHHHHCCCCEEEEEEEEEC
ENFILPISHDEVVHGKGSMITKIPGDDWQQFASLRAFYSYMWSFPGKQLVFMGQEFGQRH
CCEEEEECCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCC
EFDESVSLEWFVADLWGHGGLKRLFRDLNKIYKENPALWQLDSDPRGFEWINADDAGNNL
CCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCCCHH
FSWLRRSDDGSTIACFTNFSPNPQTDYRIDLPMEGVWTEILNTDSLEYDGTGEFGNLGQI
HHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
VAAPLPAPDRLRAVATVCVPPMGSVWLRHNPSATAALPGDPGVQ
EECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCCC
>Mature Secondary Structure 
AHDEFGGLTGWDLEGFHSGGDTEVWKRLGSHVVTIDDDERGPITGTRFAVWAPNAQAVE
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEEECCCCCCCCCCEEEEECCCCCHHH
VISDFNWWTGDRMRLIPGSGVWGTFVEGVDEGTLYKFRIQDQWGTWHEKVDPMARYSEQA
HHHCCCCCCCCEEEEECCCCCHHHHHHCCCCCCEEEEEECCCCCCHHHHCCHHHHHHHCC
PQNASIVTETHYEWNDDEWIARREASRAHAEPMSVYEVHLGGWRHGLSYRELADQLVSYV
CCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEEEEECCHHCCCCHHHHHHHHHHHH
TWQGYTHVEFMPLAEHPFAPSWGYQVTGYFSPTSRYGSPDDLRYLIDKLHQAGIGVIMDW
HCCCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEC
VPGHFPKDDWALGRFDGTALYEHADPRQGEHKDWGTYIFNYGRNEVKSFLVSSALYWISE
CCCCCCCCCCEEECCCCCEEECCCCCCCCCCCCCCHHEEECCHHHHHHHHHHHHHHHHHH
FHADGLRVDAVASMLYLDYSREEGQWVPNKYGGRENLEAIDFLRYVNSHLYSRHPGILMI
HCCCCEEHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEEE
AEESTSFPGVTKPVDDGGLGFGFKWNMGWMNDSLRYLELNPFHRQYHHGEMTFAMVYQYS
EECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEEEEEECHHHHHHCCCCEEEEEEEEEC
ENFILPISHDEVVHGKGSMITKIPGDDWQQFASLRAFYSYMWSFPGKQLVFMGQEFGQRH
CCEEEEECCCCEEECCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHCCCC
EFDESVSLEWFVADLWGHGGLKRLFRDLNKIYKENPALWQLDSDPRGFEWINADDAGNNL
CCCCCCCEEEEEEEHHCCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCEEECCCCCCCHH
FSWLRRSDDGSTIACFTNFSPNPQTDYRIDLPMEGVWTEILNTDSLEYDGTGEFGNLGQI
HHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCCCE
VAAPLPAPDRLRAVATVCVPPMGSVWLRHNPSATAALPGDPGVQ
EECCCCCHHHHHHHHHHHCCCCCCEEEEECCCCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA