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The map label for this gene is gcvH [H]

Identifier: 50842561

GI number: 50842561

Start: 1177190

End: 1177594

Strand: Direct

Name: gcvH [H]

Synonym: PPA1083

Alternate gene names: 50842561

Gene position: 1177190-1177594 (Clockwise)

Preceding gene: 50842560

Following gene: 50842562

Centisome position: 45.98

GC content: 58.52

Gene sequence:

>405_bases
ATGGAGGTGGTGATGACCGAGATTCCCGATGATGTATTGTGCACCTCGGATCACCTGTGGATTAGGCCCGGCAACGGTAG
CCGGGTGCGACTCGGAGTCACGTCAGTCCTCCTGGACCATTTTGGACCGGTGGAATCAGTGTCTCTTCCCGATGACGGTG
AGGAGGTCGTTTCCGGGGATTCCTGTGGAGACGTGGAGTTTTCCGTGCGTACCTGGGAGATTATTTCGCCAGTAGCCGGA
GAGGTTGTTGCGGTGAACCCGTCGATTGACGCTGACCAGGTTGAGGCGGACCCGTGGGGGGACGGATGGCTCTTCGACGT
GAGACTGTCAGACCCCGAGCAGTTGGACGAGTTGCTTGACCCTGACGAATACCTCGCGAGCGTGGGGGAGGTACCCCTAA
AATGA

Upstream 100 bases:

>100_bases
CCACAACCGATGACCCACGCCACACCGGTGAGATGAGGCAGGCATCAATGCTCTCGGCGCGGTACTCTGCGAGTATAAAG
ATTTTTACGTCGCCCGTGTC

Downstream 100 bases:

>100_bases
CTACTCCGGAGACCCCCGAGAGCGAGGACGCGAACATGAATAAGTGCCCCATGTGCGGCCACGTCAGTGAGGATGGCGCA
AACTTCTGCTCCCGATGTGG

Product: putative glycine cleavage system H protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 134; Mature: 134

Protein sequence:

>134_residues
MEVVMTEIPDDVLCTSDHLWIRPGNGSRVRLGVTSVLLDHFGPVESVSLPDDGEEVVSGDSCGDVEFSVRTWEIISPVAG
EVVAVNPSIDADQVEADPWGDGWLFDVRLSDPEQLDELLDPDEYLASVGEVPLK

Sequences:

>Translated_134_residues
MEVVMTEIPDDVLCTSDHLWIRPGNGSRVRLGVTSVLLDHFGPVESVSLPDDGEEVVSGDSCGDVEFSVRTWEIISPVAG
EVVAVNPSIDADQVEADPWGDGWLFDVRLSDPEQLDELLDPDEYLASVGEVPLK
>Mature_134_residues
MEVVMTEIPDDVLCTSDHLWIRPGNGSRVRLGVTSVLLDHFGPVESVSLPDDGEEVVSGDSCGDVEFSVRTWEIISPVAG
EVVAVNPSIDADQVEADPWGDGWLFDVRLSDPEQLDELLDPDEYLASVGEVPLK

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789271, Length=124, Percent_Identity=31.4516129032258, Blast_Score=74, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17507493, Length=114, Percent_Identity=30.7017543859649, Blast_Score=68, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17551294, Length=114, Percent_Identity=32.4561403508772, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6319272, Length=121, Percent_Identity=32.2314049586777, Blast_Score=65, Evalue=3e-12,
Organism=Drosophila melanogaster, GI17865652, Length=114, Percent_Identity=32.4561403508772, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14562; Mature: 14562

Theoretical pI: Translated: 3.58; Mature: 3.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVVMTEIPDDVLCTSDHLWIRPGNGSRVRLGVTSVLLDHFGPVESVSLPDDGEEVVSGD
CCEEECCCCCCEEECCCCEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCHHHHCCC
SCGDVEFSVRTWEIISPVAGEVVAVNPSIDADQVEADPWGDGWLFDVRLSDPEQLDELLD
CCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHCC
PDEYLASVGEVPLK
HHHHHHHCCCCCCC
>Mature Secondary Structure
MEVVMTEIPDDVLCTSDHLWIRPGNGSRVRLGVTSVLLDHFGPVESVSLPDDGEEVVSGD
CCEEECCCCCCEEECCCCEEEECCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCHHHHCCC
SCGDVEFSVRTWEIISPVAGEVVAVNPSIDADQVEADPWGDGWLFDVRLSDPEQLDELLD
CCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHCC
PDEYLASVGEVPLK
HHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA