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The map label for this gene is hrdD [H]

Identifier: 50842514

GI number: 50842514

Start: 1121357

End: 1122295

Strand: Reverse

Name: hrdD [H]

Synonym: PPA1031

Alternate gene names: 50842514

Gene position: 1122295-1121357 (Counterclockwise)

Preceding gene: 50842516

Following gene: 50842506

Centisome position: 43.84

GC content: 62.62

Gene sequence:

>939_bases
ATGGACACCAAGTCCCGGGTTCGTACCACCGATGGCATTGATGGTAAGGACGCAGTCGGCCTCTACCTAGACGGGATCGC
CCGCACGCCCCTACTCAACGCCGCAGAGGAGGTTGCGTTAGCCCGCCGGATCGAAGCGGGCGTGTTTGCACGCGCCATTC
TCGAAGGCCACGCGGAAACCACATCGGATACCACCACTAAGGAACTACACCAGATTGCAGCCGACGGAGACGAAGCCCTG
GCCCACTTCGTGCGCGCTAACCTGCGTCTGGTCGTGTCGGTGGCCCGCAAATACGGTCGGTCTCAGATGCCGTTACTTGA
CCTCGTGCAGGAAGGCAATACCGGGCTCATACGCGCCGTCGAGAAGTTCGACTACGCCAAGGGATTCAAGTTCTCGACCT
ACGCCACCTGGTGGGTCCGTCAGGCTATCAGCCGAGGCATCGCGCAGCAGGGTCGCATCGTCCGCCTTCCGGTGCACGTC
GCTGAACAGGTGAATCAGGTGTCGGCTGCCCGCCGCAACCTCGAGCGTCAGCTGGGTCGCGAGCCCGAAACCTTCGAAAT
CGCCGACGAGTTGGAGATCACCGAGGAGCGTGTCATCGAGCTCATCCGCTTTGGACGTGACCACGTCTCTCTCGACGCTC
CGGTTGAGGACGACGGCGACACTAGCCTGGGCGACCTCATCGCCCGCGAGACCTCTCCTGGTCCTGACGACATTGTGCTC
GACGCCGAGGACCGCAGTCGACTTGAAGGGCTCCTTGACGATCTCGACGAGCGCTCTGCCGACGTGGTGCGTCGCCGCTA
CGGACTCGTCGATGGACGCCAAGCCAAGCTTGCCGACATCGGCAAGGTGTGGGGAATCACCGCCGAACGTGTGCGTCAGA
TCGAACGTGCTGCCCTAGCCAGCATGCGTCAACGTAACCTCGCCACGATGGCGGCCTGA

Upstream 100 bases:

>100_bases
CCCACGACCGCAGACACTCATCGGCCACTCAGGGATGACATTCGACCCTCTATGACGTTGATATAGGTGTCAACCAGTCC
GACGGAGAAAGGCGAAGCAT

Downstream 100 bases:

>100_bases
CATCTGCACACGGTGGCTCCGGACCCTTCCGGGGCCACCGTCGCGTTCAGAGGACATCACGTGCCACGGCCAAGCGGAAA
CGTTACTGAGGCGTCGAGGT

Product: RNA polymerase principal sigma factor HrdD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MDTKSRVRTTDGIDGKDAVGLYLDGIARTPLLNAAEEVALARRIEAGVFARAILEGHAETTSDTTTKELHQIAADGDEAL
AHFVRANLRLVVSVARKYGRSQMPLLDLVQEGNTGLIRAVEKFDYAKGFKFSTYATWWVRQAISRGIAQQGRIVRLPVHV
AEQVNQVSAARRNLERQLGREPETFEIADELEITEERVIELIRFGRDHVSLDAPVEDDGDTSLGDLIARETSPGPDDIVL
DAEDRSRLEGLLDDLDERSADVVRRRYGLVDGRQAKLADIGKVWGITAERVRQIERAALASMRQRNLATMAA

Sequences:

>Translated_312_residues
MDTKSRVRTTDGIDGKDAVGLYLDGIARTPLLNAAEEVALARRIEAGVFARAILEGHAETTSDTTTKELHQIAADGDEAL
AHFVRANLRLVVSVARKYGRSQMPLLDLVQEGNTGLIRAVEKFDYAKGFKFSTYATWWVRQAISRGIAQQGRIVRLPVHV
AEQVNQVSAARRNLERQLGREPETFEIADELEITEERVIELIRFGRDHVSLDAPVEDDGDTSLGDLIARETSPGPDDIVL
DAEDRSRLEGLLDDLDERSADVVRRRYGLVDGRQAKLADIGKVWGITAERVRQIERAALASMRQRNLATMAA
>Mature_312_residues
MDTKSRVRTTDGIDGKDAVGLYLDGIARTPLLNAAEEVALARRIEAGVFARAILEGHAETTSDTTTKELHQIAADGDEAL
AHFVRANLRLVVSVARKYGRSQMPLLDLVQEGNTGLIRAVEKFDYAKGFKFSTYATWWVRQAISRGIAQQGRIVRLPVHV
AEQVNQVSAARRNLERQLGREPETFEIADELEITEERVIELIRFGRDHVSLDAPVEDDGDTSLGDLIARETSPGPDDIVL
DAEDRSRLEGLLDDLDERSADVVRRRYGLVDGRQAKLADIGKVWGITAERVRQIERAALASMRQRNLATMAA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=226, Percent_Identity=41.1504424778761, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789098, Length=288, Percent_Identity=38.5416666666667, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789871, Length=246, Percent_Identity=29.2682926829268, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34608; Mature: 34608

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTKSRVRTTDGIDGKDAVGLYLDGIARTPLLNAAEEVALARRIEAGVFARAILEGHAET
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSDTTTKELHQIAADGDEALAHFVRANLRLVVSVARKYGRSQMPLLDLVQEGNTGLIRAV
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHH
EKFDYAKGFKFSTYATWWVRQAISRGIAQQGRIVRLPVHVAEQVNQVSAARRNLERQLGR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCC
EPETFEIADELEITEERVIELIRFGRDHVSLDAPVEDDGDTSLGDLIARETSPGPDDIVL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHCCCCCCCCEEE
DAEDRSRLEGLLDDLDERSADVVRRRYGLVDGRQAKLADIGKVWGITAERVRQIERAALA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
SMRQRNLATMAA
HHHHHHHHHHCC
>Mature Secondary Structure
MDTKSRVRTTDGIDGKDAVGLYLDGIARTPLLNAAEEVALARRIEAGVFARAILEGHAET
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
TSDTTTKELHQIAADGDEALAHFVRANLRLVVSVARKYGRSQMPLLDLVQEGNTGLIRAV
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCHHHHHH
EKFDYAKGFKFSTYATWWVRQAISRGIAQQGRIVRLPVHVAEQVNQVSAARRNLERQLGR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHCC
EPETFEIADELEITEERVIELIRFGRDHVSLDAPVEDDGDTSLGDLIARETSPGPDDIVL
CCCCEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCHHHHHHHCCCCCCCCEEE
DAEDRSRLEGLLDDLDERSADVVRRRYGLVDGRQAKLADIGKVWGITAERVRQIERAALA
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
SMRQRNLATMAA
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3194753; 1944221; 12000953 [H]