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The map label for this gene is cysK [H]

Identifier: 50842447

GI number: 50842447

Start: 1047389

End: 1048324

Strand: Direct

Name: cysK [H]

Synonym: PPA0963

Alternate gene names: 50842447

Gene position: 1047389-1048324 (Clockwise)

Preceding gene: 50842446

Following gene: 50842449

Centisome position: 40.91

GC content: 60.47

Gene sequence:

>936_bases
ATGGCGAAGATCGCTAACGACGTTACCGAGCTCATCGGCAATACCCCGCTGGTCAAGATCAATAAACTGTTCCCGGACTC
CCAGGCCACCGTATTGGCCAAGCTCGAGTTCTATAACCCGGCCTCGAGCGTCAAGGACCGTATCGCTACGTCGATCGTTG
ACGCGGCTGAGGCTTCCGGCCAGCTCAATCCGGGAGGCACGATTGTTGAAGCCACGTCCGGTAATACAGGTGTCGGTCTG
GCTCTGGTTGGTGCGGCTCGCGGTTACAAGGTCATCATCACTATGCCTGAGACAATGTCGAAGGAGCGCCGCGCCATCAT
TCGTCTGCTGGGTGCCGAGCTGGTGCTCACCCCAGGCGCTGACGGCGTGCCAGGAGCCAACAGCAAGGCTGCCGAGATCG
TCGAGAATACCCCCGGTGCCATCCTGGCCAGCCAGTTCGATAACCCCGCTAATCCTGCGATCCATCATGATTGCACTGGT
GAGGAGATCTGGAACGATACCGAGGGCGAGGTCGACGCCATCGTCGCCGGTATCGGTACCGGTGGCACCATCTCTGGCGC
TGGCAAGTACCTCAAGGAGAAGGATCCCGAGATCAAGATCTTCGGTGCCGAGCCCGCCGAGTCCCCCGTCATTACTAAAG
GTGACAAGGCTCCGCACAAGATCCAGGGCTGGGGCCCGGGCTTTGTCCCGGATAACCTCGACAAGTCCGTCGTTGACGGG
ATCCTGCTGGTTCCCGGTGATGAGGCCATCGCCTTCGCCCGTCGCGCTGCCGCAGAAGAGGGCATTATCACGGGTATCTC
CGGTGGCGGGGCCCTGCAAGCTGCCGGTCAGGTAGTCGCTCGTCCAGAGTTTGCGGGAAAGACGATCGTCGTCGTTATCG
CTGACACTGGTGAGCGCTACCTGTCGACCGCTCTCTTCGAAGGCCTCCTCGATTGA

Upstream 100 bases:

>100_bases
TGATGCCGGTCGGAAGATTACCGCCGAAAAGACATGACCCCACACTTACGCATATCAGCAATTGCAATCACCGGATAGTC
AATCGAAAGGACTTATCACC

Downstream 100 bases:

>100_bases
GGACATATTCGTGAGGTATATGACCGGCATCGTTTGGGGTGCCGGCCATGGCATGACCCTGTTACGACGATAAGCGGCCA
TGCGTGGTTACGACACCGGC

Product: cysteine synthase

Products: NA

Alternate protein names: OAS sulfhydrylase; OASS; Cysteine synthase A; CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine-specific cysteine synthase; Sulfide-dependent cysteine synthase [H]

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MAKIANDVTELIGNTPLVKINKLFPDSQATVLAKLEFYNPASSVKDRIATSIVDAAEASGQLNPGGTIVEATSGNTGVGL
ALVGAARGYKVIITMPETMSKERRAIIRLLGAELVLTPGADGVPGANSKAAEIVENTPGAILASQFDNPANPAIHHDCTG
EEIWNDTEGEVDAIVAGIGTGGTISGAGKYLKEKDPEIKIFGAEPAESPVITKGDKAPHKIQGWGPGFVPDNLDKSVVDG
ILLVPGDEAIAFARRAAAEEGIITGISGGGALQAAGQVVARPEFAGKTIVVVIADTGERYLSTALFEGLLD

Sequences:

>Translated_311_residues
MAKIANDVTELIGNTPLVKINKLFPDSQATVLAKLEFYNPASSVKDRIATSIVDAAEASGQLNPGGTIVEATSGNTGVGL
ALVGAARGYKVIITMPETMSKERRAIIRLLGAELVLTPGADGVPGANSKAAEIVENTPGAILASQFDNPANPAIHHDCTG
EEIWNDTEGEVDAIVAGIGTGGTISGAGKYLKEKDPEIKIFGAEPAESPVITKGDKAPHKIQGWGPGFVPDNLDKSVVDG
ILLVPGDEAIAFARRAAAEEGIITGISGGGALQAAGQVVARPEFAGKTIVVVIADTGERYLSTALFEGLLD
>Mature_310_residues
AKIANDVTELIGNTPLVKINKLFPDSQATVLAKLEFYNPASSVKDRIATSIVDAAEASGQLNPGGTIVEATSGNTGVGLA
LVGAARGYKVIITMPETMSKERRAIIRLLGAELVLTPGADGVPGANSKAAEIVENTPGAILASQFDNPANPAIHHDCTGE
EIWNDTEGEVDAIVAGIGTGGTISGAGKYLKEKDPEIKIFGAEPAESPVITKGDKAPHKIQGWGPGFVPDNLDKSVVDGI
LLVPGDEAIAFARRAAAEEGIITGISGGGALQAAGQVVARPEFAGKTIVVVIADTGERYLSTALFEGLLD

Specific function: Catalyzes the conversion of O-acetylserine (OAS) to cysteine through the elimination of acetate and addition of hydrogen sulfide [H]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=314, Percent_Identity=39.8089171974522, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI295821200, Length=314, Percent_Identity=39.8089171974522, Blast_Score=221, Evalue=9e-58,
Organism=Homo sapiens, GI4557415, Length=314, Percent_Identity=39.8089171974522, Blast_Score=221, Evalue=9e-58,
Organism=Escherichia coli, GI1788754, Length=319, Percent_Identity=49.2163009404389, Blast_Score=269, Evalue=2e-73,
Organism=Escherichia coli, GI2367138, Length=300, Percent_Identity=41, Blast_Score=224, Evalue=6e-60,
Organism=Caenorhabditis elegans, GI17535051, Length=303, Percent_Identity=50.1650165016502, Blast_Score=302, Evalue=2e-82,
Organism=Caenorhabditis elegans, GI17562970, Length=309, Percent_Identity=47.2491909385113, Blast_Score=291, Evalue=4e-79,
Organism=Caenorhabditis elegans, GI32566674, Length=299, Percent_Identity=48.1605351170569, Blast_Score=285, Evalue=3e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=304, Percent_Identity=49.0131578947368, Blast_Score=282, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI17561720, Length=308, Percent_Identity=44.1558441558442, Blast_Score=250, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI32566672, Length=217, Percent_Identity=46.5437788018433, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=320, Percent_Identity=37.5, Blast_Score=167, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI25147552, Length=316, Percent_Identity=34.8101265822785, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17561716, Length=124, Percent_Identity=40.3225806451613, Blast_Score=98, Evalue=7e-21,
Organism=Saccharomyces cerevisiae, GI6321594, Length=316, Percent_Identity=40.1898734177215, Blast_Score=218, Evalue=8e-58,
Organism=Saccharomyces cerevisiae, GI6321449, Length=326, Percent_Identity=34.9693251533742, Blast_Score=140, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24643623, Length=320, Percent_Identity=39.0625, Blast_Score=194, Evalue=6e-50,
Organism=Drosophila melanogaster, GI20129101, Length=320, Percent_Identity=39.0625, Blast_Score=194, Evalue=6e-50,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 31958; Mature: 31826

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIANDVTELIGNTPLVKINKLFPDSQATVLAKLEFYNPASSVKDRIATSIVDAAEASG
CCCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCC
QLNPGGTIVEATSGNTGVGLALVGAARGYKVIITMPETMSKERRAIIRLLGAELVLTPGA
CCCCCCCEEEECCCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEECCC
DGVPGANSKAAEIVENTPGAILASQFDNPANPAIHHDCTGEEIWNDTEGEVDAIVAGIGT
CCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHCCCCCCCEEEEEEECCC
GGTISGAGKYLKEKDPEIKIFGAEPAESPVITKGDKAPHKIQGWGPGFVPDNLDKSVVDG
CCCCCCCHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHCC
ILLVPGDEAIAFARRAAAEEGIITGISGGGALQAAGQVVARPEFAGKTIVVVIADTGERY
EEEECCCHHHHHHHHHHHCCCCEEEECCCCHHHHCCCEEECCCCCCCEEEEEEECCCHHH
LSTALFEGLLD
HHHHHHHHHCC
>Mature Secondary Structure 
AKIANDVTELIGNTPLVKINKLFPDSQATVLAKLEFYNPASSVKDRIATSIVDAAEASG
CCHHHHHHHHHCCCCEEEEEECCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHCC
QLNPGGTIVEATSGNTGVGLALVGAARGYKVIITMPETMSKERRAIIRLLGAELVLTPGA
CCCCCCCEEEECCCCCCCEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHCCCEEEECCC
DGVPGANSKAAEIVENTPGAILASQFDNPANPAIHHDCTGEEIWNDTEGEVDAIVAGIGT
CCCCCCCCHHHHHHHCCCCEEEEECCCCCCCCCEECCCCCHHHCCCCCCCEEEEEEECCC
GGTISGAGKYLKEKDPEIKIFGAEPAESPVITKGDKAPHKIQGWGPGFVPDNLDKSVVDG
CCCCCCCHHHHHCCCCCEEEEECCCCCCCCEECCCCCCCCCCCCCCCCCCCCCCHHHHCC
ILLVPGDEAIAFARRAAAEEGIITGISGGGALQAAGQVVARPEFAGKTIVVVIADTGERY
EEEECCCHHHHHHHHHHHCCCCEEEECCCCHHHHCCCEEECCCCCCCEEEEEEECCCHHH
LSTALFEGLLD
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]