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The map label for this gene is menD [H]

Identifier: 50842387

GI number: 50842387

Start: 984221

End: 985837

Strand: Direct

Name: menD [H]

Synonym: PPA0903

Alternate gene names: 50842387

Gene position: 984221-985837 (Clockwise)

Preceding gene: 50842386

Following gene: 50842393

Centisome position: 38.44

GC content: 66.91

Gene sequence:

>1617_bases
TTGAGGAGTCGGATACGGATCTGTGTCCGAGGTGGTCACAGCGTTTGGCTCTCACCGTGGCCGCCCTTGTTGAGCGAGAA
CGGAGTTGAGGTGAACCCCTCTACAGCAGTCGCCCGACGTCTGGTGAATGCTCTCGTTGAGGCCGGGGTCGAACACGTCG
TGTATTGCCCCGGATCCCGGGATGCCCCTATTGGGTATGCGTTGGCTGATGCTGAAACTGCTGGATGGCTGCGGGTTTAC
GTGCGTCTGGACGAGAGGTCAGCTGGTTTCGTCGCCCTAGGGCTGGGCCGGGCTGGCTGCCCGGCTGCGTTAGTCACGAC
CTCGGGCACCGCGGTGGCCAACATTCACCCGGCCGTTCTCGAGGCCGACGCTGCCGGGGTGCCGCTTATCGTCCTCTCGG
CCGACCGTCCCGCCGAGATGTGGCACACGGGGGCTAACCAGACGACTGTCCAAACCGGGATCTTCGGGTCCGCGCCACGT
CTCAGCACTGATGTGCCGGCCGGATTCCCCGCCGACGAACGCCTCGACGCTCTTGTTCTACGGGCTGTGACAGCCGCGAC
CGGGGCGCTGAGCGCCGACCCGGGCCCGGTGCACCTCAACGTGAGCTTTCGTGACTCGCTCGTGCCTGACGGGCCGTGGC
AGCCGCAGGCATTGGTGCCCCGTCGGGTGAGTTCCTTCCCGACTGCACCGACGCCGCTGGTGATGCCTGCCCGGACGGTC
GTCGTGGCCGGGGACGGTGCCGGATCGCTCGCCCGCGAGTTGGCACAGCAGGGCGGATGGCCTCTGCTGGCCGAACCCAC
CTCGGGCTCCCGGGTCGGGGACAACGCTCTCACCGACTACCAGACGGTCCTGGGTAGCGAGCTGGTCGACGATGTCGAGG
CTGTCCTCGTCCTGGGCCACCCCACTTTGTCGCGCCCGGTGTCGCGGCTGTTAGCGCGTCCCGACGTTACCGTCGTAACT
GATCGCAGTCGGTGGACTGACGTCGCCGGGGTAGCCCGGGTCGTCACGGGACCGGTGGAACTGGCCGAGATCGATACCGA
CCCGGCGTGGCTCGGCCGCTGGAAGGACGCAGACCGGCCCGTCGTACCGACCGCCAAGCAGCGCCTGTGCCGCGACATCT
GGTGGGCCTGTACGACCCCGAACGCCCCCGTGCTTGTCATTGGCGCCTCCGAAGTCATCCGCTGTTTCGATCGATTTGCT
GTGCCCGGCGACATCGCTCCAACGGCGCTGGCCAACCGGGGGCTGGCAGGCATCGACGGCACCATCGCTACGGCGATCGG
TGTGGGGTTGGGCACCGGTCGGCCGGTGCGTGTTGTCGTCGGCGATCTCACCTTCGCCCACGACGCCATGTCCCTGCTGT
TGGGAGAGACCGAACCTGAACCTGACGTGCAGGTTGTCGTCCTGGACGACCGTGGTGGGGCGATTTTTAGCGGCCTTGAG
CACGCCGCTGCCCCCGCGCCTGTGCTGCGACGGATGTTTTTGACCCCTCAGCGTCTCGATTCCGCCGCCCTCGCGCACGC
CCTCGGAGCGAGTCACCGGAGGGTCTCTCCAGACGACCTCCAGTTCCTCGACGAACCGGTTGTGGGTCGTCAGGTCGTTT
CGGTACCACTCACCTGA

Upstream 100 bases:

>100_bases
CTGGGGACAATTCGTCTTTTTCAGGGTGATGTCAGCGCCTCGCCACTCCTTCCGGTTGACGGTTACCTGAGACCGCGGCG
CGGGGAGGTTGACGTCGAGG

Downstream 100 bases:

>100_bases
CCGTTGGGCAGGGCGTCGCCGAAGGCTTCCATCTTGGCGCCAACCTCGATCCACTCCTTAGCAGGGTCAGAGGTTGGCAA
GATTCCGGCTCCAGCGAACA

Product: menaquinone biosynthesis protein

Products: NA

Alternate protein names: SEPHCHC synthase; Menaquinone biosynthesis protein menD [H]

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MRSRIRICVRGGHSVWLSPWPPLLSENGVEVNPSTAVARRLVNALVEAGVEHVVYCPGSRDAPIGYALADAETAGWLRVY
VRLDERSAGFVALGLGRAGCPAALVTTSGTAVANIHPAVLEADAAGVPLIVLSADRPAEMWHTGANQTTVQTGIFGSAPR
LSTDVPAGFPADERLDALVLRAVTAATGALSADPGPVHLNVSFRDSLVPDGPWQPQALVPRRVSSFPTAPTPLVMPARTV
VVAGDGAGSLARELAQQGGWPLLAEPTSGSRVGDNALTDYQTVLGSELVDDVEAVLVLGHPTLSRPVSRLLARPDVTVVT
DRSRWTDVAGVARVVTGPVELAEIDTDPAWLGRWKDADRPVVPTAKQRLCRDIWWACTTPNAPVLVIGASEVIRCFDRFA
VPGDIAPTALANRGLAGIDGTIATAIGVGLGTGRPVRVVVGDLTFAHDAMSLLLGETEPEPDVQVVVLDDRGGAIFSGLE
HAAAPAPVLRRMFLTPQRLDSAALAHALGASHRRVSPDDLQFLDEPVVGRQVVSVPLT

Sequences:

>Translated_538_residues
MRSRIRICVRGGHSVWLSPWPPLLSENGVEVNPSTAVARRLVNALVEAGVEHVVYCPGSRDAPIGYALADAETAGWLRVY
VRLDERSAGFVALGLGRAGCPAALVTTSGTAVANIHPAVLEADAAGVPLIVLSADRPAEMWHTGANQTTVQTGIFGSAPR
LSTDVPAGFPADERLDALVLRAVTAATGALSADPGPVHLNVSFRDSLVPDGPWQPQALVPRRVSSFPTAPTPLVMPARTV
VVAGDGAGSLARELAQQGGWPLLAEPTSGSRVGDNALTDYQTVLGSELVDDVEAVLVLGHPTLSRPVSRLLARPDVTVVT
DRSRWTDVAGVARVVTGPVELAEIDTDPAWLGRWKDADRPVVPTAKQRLCRDIWWACTTPNAPVLVIGASEVIRCFDRFA
VPGDIAPTALANRGLAGIDGTIATAIGVGLGTGRPVRVVVGDLTFAHDAMSLLLGETEPEPDVQVVVLDDRGGAIFSGLE
HAAAPAPVLRRMFLTPQRLDSAALAHALGASHRRVSPDDLQFLDEPVVGRQVVSVPLT
>Mature_538_residues
MRSRIRICVRGGHSVWLSPWPPLLSENGVEVNPSTAVARRLVNALVEAGVEHVVYCPGSRDAPIGYALADAETAGWLRVY
VRLDERSAGFVALGLGRAGCPAALVTTSGTAVANIHPAVLEADAAGVPLIVLSADRPAEMWHTGANQTTVQTGIFGSAPR
LSTDVPAGFPADERLDALVLRAVTAATGALSADPGPVHLNVSFRDSLVPDGPWQPQALVPRRVSSFPTAPTPLVMPARTV
VVAGDGAGSLARELAQQGGWPLLAEPTSGSRVGDNALTDYQTVLGSELVDDVEAVLVLGHPTLSRPVSRLLARPDVTVVT
DRSRWTDVAGVARVVTGPVELAEIDTDPAWLGRWKDADRPVVPTAKQRLCRDIWWACTTPNAPVLVIGASEVIRCFDRFA
VPGDIAPTALANRGLAGIDGTIATAIGVGLGTGRPVRVVVGDLTFAHDAMSLLLGETEPEPDVQVVVLDDRGGAIFSGLE
HAAAPAPVLRRMFLTPQRLDSAALAHALGASHRRVSPDDLQFLDEPVVGRQVVSVPLT

Specific function: Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3- cyclohexene-1-ca

COG id: COG1165

COG function: function code H; 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family. MenD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788599, Length=551, Percent_Identity=28.8566243194192, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004433
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.9 [H]

Molecular weight: Translated: 56458; Mature: 56458

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSRIRICVRGGHSVWLSPWPPLLSENGVEVNPSTAVARRLVNALVEAGVEHVVYCPGSR
CCCCEEEEEECCCEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEECCCCC
DAPIGYALADAETAGWLRVYVRLDERSAGFVALGLGRAGCPAALVTTSGTAVANIHPAVL
CCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCEEEECCHHHE
EADAAGVPLIVLSADRPAEMWHTGANQTTVQTGIFGSAPRLSTDVPAGFPADERLDALVL
ECCCCCCCEEEEECCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
RAVTAATGALSADPGPVHLNVSFRDSLVPDGPWQPQALVPRRVSSFPTAPTPLVMPARTV
HHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHHCCCCCCCCEEECCEEE
VVAGDGAGSLARELAQQGGWPLLAEPTSGSRVGDNALTDYQTVLGSELVDDVEAVLVLGH
EEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
PTLSRPVSRLLARPDVTVVTDRSRWTDVAGVARVVTGPVELAEIDTDPAWLGRWKDADRP
CHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCCCCC
VVPTAKQRLCRDIWWACTTPNAPVLVIGASEVIRCFDRFAVPGDIAPTALANRGLAGIDG
CCCHHHHHHHHHHHEEECCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCH
TIATAIGVGLGTGRPVRVVVGDLTFAHDAMSLLLGETEPEPDVQVVVLDDRGGAIFSGLE
HHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHH
HAAAPAPVLRRMFLTPQRLDSAALAHALGASHRRVSPDDLQFLDEPVVGRQVVSVPLT
HHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCC
>Mature Secondary Structure
MRSRIRICVRGGHSVWLSPWPPLLSENGVEVNPSTAVARRLVNALVEAGVEHVVYCPGSR
CCCCEEEEEECCCEEEECCCCCCCCCCCCEECCHHHHHHHHHHHHHHCCCCEEEECCCCC
DAPIGYALADAETAGWLRVYVRLDERSAGFVALGLGRAGCPAALVTTSGTAVANIHPAVL
CCCCEEEEECCCCCCEEEEEEEECCCCCCEEEEECCCCCCCEEEEEECCCEEEECCHHHE
EADAAGVPLIVLSADRPAEMWHTGANQTTVQTGIFGSAPRLSTDVPAGFPADERLDALVL
ECCCCCCCEEEEECCCCHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHH
RAVTAATGALSADPGPVHLNVSFRDSLVPDGPWQPQALVPRRVSSFPTAPTPLVMPARTV
HHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHHCCCCCCCCEEECCEEE
VVAGDGAGSLARELAQQGGWPLLAEPTSGSRVGDNALTDYQTVLGSELVDDVEAVLVLGH
EEECCCCHHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHEEEEECC
PTLSRPVSRLLARPDVTVVTDRSRWTDVAGVARVVTGPVELAEIDTDPAWLGRWKDADRP
CHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCHHHHCCCCCCCCC
VVPTAKQRLCRDIWWACTTPNAPVLVIGASEVIRCFDRFAVPGDIAPTALANRGLAGIDG
CCCHHHHHHHHHHHEEECCCCCCEEEECHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCH
TIATAIGVGLGTGRPVRVVVGDLTFAHDAMSLLLGETEPEPDVQVVVLDDRGGAIFSGLE
HHHHHHHHCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCHHHHHHH
HAAAPAPVLRRMFLTPQRLDSAALAHALGASHRRVSPDDLQFLDEPVVGRQVVSVPLT
HHCCCHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA