Click here to switch to the map view.

The map label for this gene is lepA

Identifier: 50842385

GI number: 50842385

Start: 981470

End: 983305

Strand: Direct

Name: lepA

Synonym: PPA0901

Alternate gene names: 50842385

Gene position: 981470-983305 (Clockwise)

Preceding gene: 50842383

Following gene: 50842386

Centisome position: 38.33

GC content: 57.84

Gene sequence:

>1836_bases
ATGTCCGCGCCTCAGCCTGGCAGTACAGATCCGGCCGTCATCCGGAACTTCTGTATCATTGCACACATTGACCACGGCAA
GTCGACCCTAGCTGATCGGATGTTGCAAATCACTGGTGTCCTTGACGAGCGCAGTGCTCGCGCCCAGTACTTAGACCGCA
TGGATATTGAGCGGGAACGCGGAATCACCATCAAGTCCCAGGCAGTGCGGATGCCGTGGGAGGTTGACGGTGTCACTCAC
CTTCTCAACATGATCGACACTCCTGGGCACGTCGACTTCTCCTACGAGGTATCCAGATCTCTGCAGGCCTGTGAAGGAGC
GATTTTGCTCGTCGACGCGGCTCAGGGCATCGAAGCCCAGACGTTAGCCAACCTGTATCTGGCTTTGGAAGCTGATCTGG
AGATCATTCCGGTGCTCAACAAGATCGACTTGCCTGGTGCTGAGTCGGATCGTCATGCCGCCGAGATTGCCGGAATCATT
GGTTGCGACGAGTCTGAGGTGCTGCGAGTTTCGGCAAAGACTGGTGAGGGCGTCAGCGATCTGCTTGATACCATCGTCGC
TAAGGTGCCAGCCCCCGAGGGAGTGGCCGACGCGCCTGCTCGGGCCCTCATTTTTGACTCGGTCTATGACACGTATCGTG
GTGTTGTCACGTATGTCCGCGTCGTTGACGGTGCTCTGCGCCACCGTGAGAAAATCCTTATGATGAGTACCGGCGCAGCA
CACGAGGTGCTTGAGATTGGCGTCATATCCCCAGAAATGGTGCCGGCTCAGGGGCTGTCGGTGGGGGAGGTCGGGTACCT
CATCACCGGCGTCAAGGATGTCCGTCAATCCCGAGTCGGCGACACTGTCACCAATGCCTCCAAGCCCTCTGAGAAAGATC
TTGGCGGCTATCAGCACCCCAAACCAATGGTGTATTCGGGACTCTTCCCGATTGATGCCAAGGATTTCCCTGACTTGCGT
GATGCTCTCGACAAGTTGCAGCTCAACGACGCTGCCCTGGTCTACGAGCCTGAAACCTCGACGGCCCTGGGCTTTGGCTT
TCGGGTCGGGTTCCTCGGGCTGCTGCACATGGAGATCGTGCGCGAGCGTCTAGAGCGCGAGTTCGACCTTGACCTCATCT
CCACAGCTCCCTCAGTGGTTCACCACGTCCTCATGGAAGACGGATCGACTGTCGCCGTGACAAACCCGTCGGAGTACCCG
ACTAGCGGGCGGATCGCTGAGGTGCGTGAACCCATCGTTGACGCCACCATTCTCAGTCCTGCCGAGTACATCGGCACCAT
CCTTGAGTTGTGTCAGCAGCGTCGTGGTGTTCAGCAAGGCTTGGATTATTTGTCGTCTGATCGAGTGGAAATCCGGTACC
GTCTGCCGCTTTCGGAGATCGTTTTCGACTTCTTTGACCAGCTCAAGTCGCGAACCAAAGGGTATGCCTCGCTGGACTAC
CACGAAGCCGGTGAGCAGGCTGCCGACCTCGTTAAAGTCGACATTTTGCTCAACGGAGATCCGGTGGACGCTTTAAGCTC
GATCGTCCACCGCGACAAGTCTTATTCCTACGGCGTCGCGATGGCCGCCAAGCTTAAGGAACTCATCCCGCGCCAGCAGT
TCGAGGTGCCGGTCCAGGCCGCCATTGGCGCGCGGGTTATCGCTCGTGAGACCATTCGCGCCGTTCGTAAGGACGTGCTG
GCCAAATGCTATGGCGGTGACATTTCCCGTAAGCGCAAGCTGCTTGAGAAGCAGAAGGCTGGTAAGAAGCGCATGAAGGT
CGTCGGCTCGGTGGAAGTGCCTCAGGAGGCCTTTGTAGCTGCCTTGCGTACCGGAGAGTCCACGGAGAAAAAGTGA

Upstream 100 bases:

>100_bases
ACGGTGTGATCGTAACTGAAAGCACAGTGTCACTGGCGCGTGAGAGACTACATGTTGAGCCGGGCATCCCCGGCACCATC
ATCGATATATGAGGGAGCAG

Downstream 100 bases:

>100_bases
AGGGTCTGAAGGCGTACCGATTAGACGTTCCGCCCATGCTGGCCGATCGTGGTATTGATCGGATGCTCGCATTCGCCGTG
CCTCTGACAGTGAGGTTCCG

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA

Number of amino acids: Translated: 611; Mature: 610

Protein sequence:

>611_residues
MSAPQPGSTDPAVIRNFCIIAHIDHGKSTLADRMLQITGVLDERSARAQYLDRMDIERERGITIKSQAVRMPWEVDGVTH
LLNMIDTPGHVDFSYEVSRSLQACEGAILLVDAAQGIEAQTLANLYLALEADLEIIPVLNKIDLPGAESDRHAAEIAGII
GCDESEVLRVSAKTGEGVSDLLDTIVAKVPAPEGVADAPARALIFDSVYDTYRGVVTYVRVVDGALRHREKILMMSTGAA
HEVLEIGVISPEMVPAQGLSVGEVGYLITGVKDVRQSRVGDTVTNASKPSEKDLGGYQHPKPMVYSGLFPIDAKDFPDLR
DALDKLQLNDAALVYEPETSTALGFGFRVGFLGLLHMEIVRERLEREFDLDLISTAPSVVHHVLMEDGSTVAVTNPSEYP
TSGRIAEVREPIVDATILSPAEYIGTILELCQQRRGVQQGLDYLSSDRVEIRYRLPLSEIVFDFFDQLKSRTKGYASLDY
HEAGEQAADLVKVDILLNGDPVDALSSIVHRDKSYSYGVAMAAKLKELIPRQQFEVPVQAAIGARVIARETIRAVRKDVL
AKCYGGDISRKRKLLEKQKAGKKRMKVVGSVEVPQEAFVAALRTGESTEKK

Sequences:

>Translated_611_residues
MSAPQPGSTDPAVIRNFCIIAHIDHGKSTLADRMLQITGVLDERSARAQYLDRMDIERERGITIKSQAVRMPWEVDGVTH
LLNMIDTPGHVDFSYEVSRSLQACEGAILLVDAAQGIEAQTLANLYLALEADLEIIPVLNKIDLPGAESDRHAAEIAGII
GCDESEVLRVSAKTGEGVSDLLDTIVAKVPAPEGVADAPARALIFDSVYDTYRGVVTYVRVVDGALRHREKILMMSTGAA
HEVLEIGVISPEMVPAQGLSVGEVGYLITGVKDVRQSRVGDTVTNASKPSEKDLGGYQHPKPMVYSGLFPIDAKDFPDLR
DALDKLQLNDAALVYEPETSTALGFGFRVGFLGLLHMEIVRERLEREFDLDLISTAPSVVHHVLMEDGSTVAVTNPSEYP
TSGRIAEVREPIVDATILSPAEYIGTILELCQQRRGVQQGLDYLSSDRVEIRYRLPLSEIVFDFFDQLKSRTKGYASLDY
HEAGEQAADLVKVDILLNGDPVDALSSIVHRDKSYSYGVAMAAKLKELIPRQQFEVPVQAAIGARVIARETIRAVRKDVL
AKCYGGDISRKRKLLEKQKAGKKRMKVVGSVEVPQEAFVAALRTGESTEKK
>Mature_610_residues
SAPQPGSTDPAVIRNFCIIAHIDHGKSTLADRMLQITGVLDERSARAQYLDRMDIERERGITIKSQAVRMPWEVDGVTHL
LNMIDTPGHVDFSYEVSRSLQACEGAILLVDAAQGIEAQTLANLYLALEADLEIIPVLNKIDLPGAESDRHAAEIAGIIG
CDESEVLRVSAKTGEGVSDLLDTIVAKVPAPEGVADAPARALIFDSVYDTYRGVVTYVRVVDGALRHREKILMMSTGAAH
EVLEIGVISPEMVPAQGLSVGEVGYLITGVKDVRQSRVGDTVTNASKPSEKDLGGYQHPKPMVYSGLFPIDAKDFPDLRD
ALDKLQLNDAALVYEPETSTALGFGFRVGFLGLLHMEIVRERLEREFDLDLISTAPSVVHHVLMEDGSTVAVTNPSEYPT
SGRIAEVREPIVDATILSPAEYIGTILELCQQRRGVQQGLDYLSSDRVEIRYRLPLSEIVFDFFDQLKSRTKGYASLDYH
EAGEQAADLVKVDILLNGDPVDALSSIVHRDKSYSYGVAMAAKLKELIPRQQFEVPVQAAIGARVIARETIRAVRKDVLA
KCYGGDISRKRKLLEKQKAGKKRMKVVGSVEVPQEAFVAALRTGESTEKK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily

Homologues:

Organism=Homo sapiens, GI157426893, Length=602, Percent_Identity=47.3421926910299, Blast_Score=624, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=43.7037037037037, Blast_Score=123, Evalue=5e-28,
Organism=Homo sapiens, GI4503483, Length=145, Percent_Identity=38.6206896551724, Blast_Score=104, Evalue=3e-22,
Organism=Homo sapiens, GI25306287, Length=147, Percent_Identity=44.2176870748299, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI25306283, Length=147, Percent_Identity=44.2176870748299, Blast_Score=103, Evalue=5e-22,
Organism=Homo sapiens, GI19923640, Length=147, Percent_Identity=44.2176870748299, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI310132016, Length=112, Percent_Identity=40.1785714285714, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310110807, Length=112, Percent_Identity=40.1785714285714, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI310123363, Length=112, Percent_Identity=40.1785714285714, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI18390331, Length=144, Percent_Identity=37.5, Blast_Score=98, Evalue=2e-20,
Organism=Homo sapiens, GI217272892, Length=135, Percent_Identity=34.8148148148148, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI217272894, Length=135, Percent_Identity=34.8148148148148, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI94966752, Length=134, Percent_Identity=30.5970149253731, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI53729339, Length=229, Percent_Identity=27.5109170305677, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=229, Percent_Identity=27.5109170305677, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1788922, Length=597, Percent_Identity=55.9463986599665, Blast_Score=666, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=513, Percent_Identity=25.7309941520468, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1789738, Length=164, Percent_Identity=37.1951219512195, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1789559, Length=279, Percent_Identity=30.4659498207885, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790835, Length=136, Percent_Identity=33.8235294117647, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1790412, Length=290, Percent_Identity=27.9310344827586, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789737, Length=290, Percent_Identity=27.9310344827586, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=40.5228758169935, Blast_Score=483, Evalue=1e-136,
Organism=Caenorhabditis elegans, GI71988811, Length=168, Percent_Identity=37.5, Blast_Score=105, Evalue=6e-23,
Organism=Caenorhabditis elegans, GI71988819, Length=168, Percent_Identity=37.5, Blast_Score=105, Evalue=7e-23,
Organism=Caenorhabditis elegans, GI17533571, Length=149, Percent_Identity=40.2684563758389, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17556745, Length=133, Percent_Identity=38.3458646616541, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=35.4838709677419, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17552882, Length=136, Percent_Identity=36.0294117647059, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323320, Length=598, Percent_Identity=45.9866220735786, Blast_Score=545, Evalue=1e-155,
Organism=Saccharomyces cerevisiae, GI6324707, Length=143, Percent_Identity=40.5594405594406, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=143, Percent_Identity=40.5594405594406, Blast_Score=110, Evalue=9e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=201, Percent_Identity=31.8407960199005, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324166, Length=146, Percent_Identity=42.4657534246575, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6322359, Length=111, Percent_Identity=43.2432432432432, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI78706572, Length=598, Percent_Identity=44.8160535117057, Blast_Score=543, Evalue=1e-154,
Organism=Drosophila melanogaster, GI28574573, Length=136, Percent_Identity=44.1176470588235, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=38.7755102040816, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582462, Length=144, Percent_Identity=38.8888888888889, Blast_Score=100, Evalue=5e-21,
Organism=Drosophila melanogaster, GI221458488, Length=146, Percent_Identity=39.7260273972603, Blast_Score=92, Evalue=8e-19,
Organism=Drosophila melanogaster, GI21357743, Length=138, Percent_Identity=36.231884057971, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28572034, Length=248, Percent_Identity=27.8225806451613, Blast_Score=69, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEPA_PROAC (Q6A9B2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055612.1
- ProteinModelPortal:   Q6A9B2
- SMR:   Q6A9B2
- GeneID:   2930896
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0901
- NMPDR:   fig|267747.1.peg.889
- HOGENOM:   HBG286375
- OMA:   YDSYRGV
- ProtClustDB:   PRK05433
- BioCyc:   PACN267747:PPA0901-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00071
- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000
- Gene3D:   G3DSA:3.30.70.240
- PRINTS:   PR00315
- SMART:   SM00838
- TIGRFAMs:   TIGR01393
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 66869; Mature: 66738

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPQPGSTDPAVIRNFCIIAHIDHGKSTLADRMLQITGVLDERSARAQYLDRMDIERER
CCCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
GITIKSQAVRMPWEVDGVTHLLNMIDTPGHVDFSYEVSRSLQACEGAILLVDAAQGIEAQ
CCEEECCCEECCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCEEEEEECCCCCCHH
TLANLYLALEADLEIIPVLNKIDLPGAESDRHAAEIAGIIGCDESEVLRVSAKTGEGVSD
HHHHHHEEEECCCEEEECHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHH
LLDTIVAKVPAPEGVADAPARALIFDSVYDTYRGVVTYVRVVDGALRHREKILMMSTGAA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCH
HEVLEIGVISPEMVPAQGLSVGEVGYLITGVKDVRQSRVGDTVTNASKPSEKDLGGYQHP
HHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCC
KPMVYSGLFPIDAKDFPDLRDALDKLQLNDAALVYEPETSTALGFGFRVGFLGLLHMEIV
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RERLEREFDLDLISTAPSVVHHVLMEDGSTVAVTNPSEYPTSGRIAEVREPIVDATILSP
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCH
AEYIGTILELCQQRRGVQQGLDYLSSDRVEIRYRLPLSEIVFDFFDQLKSRTKGYASLDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCH
HEAGEQAADLVKVDILLNGDPVDALSSIVHRDKSYSYGVAMAAKLKELIPRQQFEVPVQA
HHHHHHHHCEEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCHHH
AIGARVIARETIRAVRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKVVGSVEVPQEAFVA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ALRTGESTEKK
HHHCCCCCCCC
>Mature Secondary Structure 
SAPQPGSTDPAVIRNFCIIAHIDHGKSTLADRMLQITGVLDERSARAQYLDRMDIERER
CCCCCCCCCHHHHHCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHC
GITIKSQAVRMPWEVDGVTHLLNMIDTPGHVDFSYEVSRSLQACEGAILLVDAAQGIEAQ
CCEEECCCEECCCCHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHCCEEEEEECCCCCCHH
TLANLYLALEADLEIIPVLNKIDLPGAESDRHAAEIAGIIGCDESEVLRVSAKTGEGVSD
HHHHHHEEEECCCEEEECHHCCCCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHH
LLDTIVAKVPAPEGVADAPARALIFDSVYDTYRGVVTYVRVVDGALRHREKILMMSTGAA
HHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCH
HEVLEIGVISPEMVPAQGLSVGEVGYLITGVKDVRQSRVGDTVTNASKPSEKDLGGYQHP
HHHHEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHCCCCCCCCCCCCCCCCC
KPMVYSGLFPIDAKDFPDLRDALDKLQLNDAALVYEPETSTALGFGFRVGFLGLLHMEIV
CCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHH
RERLEREFDLDLISTAPSVVHHVLMEDGSTVAVTNPSEYPTSGRIAEVREPIVDATILSP
HHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCH
AEYIGTILELCQQRRGVQQGLDYLSSDRVEIRYRLPLSEIVFDFFDQLKSRTKGYASLDY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCH
HEAGEQAADLVKVDILLNGDPVDALSSIVHRDKSYSYGVAMAAKLKELIPRQQFEVPVQA
HHHHHHHHCEEEEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHCCCCHHH
AIGARVIARETIRAVRKDVLAKCYGGDISRKRKLLEKQKAGKKRMKVVGSVEVPQEAFVA
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHH
ALRTGESTEKK
HHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA