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The map label for this gene is pcrA [H]

Identifier: 50842338

GI number: 50842338

Start: 929874

End: 931562

Strand: Direct

Name: pcrA [H]

Synonym: PPA0852

Alternate gene names: 50842338

Gene position: 929874-931562 (Clockwise)

Preceding gene: 50842337

Following gene: 50842341

Centisome position: 36.32

GC content: 56.6

Gene sequence:

>1689_bases
ATGGTCAGCCCGACTCAGAAGCAGATTGACATTAGGGACACGCGGGTTCTCGATCTTCTTGTCGTTGCTCCTGCGGGATG
CGGCAAGACGGAAGCGCTTGCATTACGAATCCAAGGACTTCTTCACCGCGGCGATGTGACTGCCCCAAAGAAGATCTTGG
TGACAACATTCTCGAACCGAGCCCGCGACAACATCAGAGAACGATTGGGGTCCTACCTGACGCCTTCGGTAATGCGCGAC
CGCGTTACTGTTGTGAACTTTCATGGACTGGCAGCCCGGTTGATCAGAGCGCACGGAAATGTTATCGGCCTAAGTGAGGA
ACTACGCCTTCCGGAGAACGACTGGGTTGGCGAGCAACTCCGCCAACGCAACACCTCCTGGAATGACCGAGGAGCGATCC
AGAATGCGCTTCGTGAGACAAAGCAACAACCTTTCAGCGACGCAGCGGTCGAAGCTGAACTTGAACGACTCGGAAATGTC
ACTGCACTTGAACTGGAACGGCTCAGAAAGGAAGAAGGACGCCTCACCTATGATGATCTGCCAAGACTTGCCGAGCTGAT
TCTCGGTTACGAGAGCGTCGCGGACCTCTACGCGACACACTTCGGCGCTGTCGTGGTTGACGAATTCCAAGACCTGACTC
CCCAGCAACTCCGAATCGTAAACAGAATCGGTTACAAGCGCACAACCTATGGCGGTGACCTTGTGCAGGGGATCTATGGT
TTTGCAGGAGCGCAGCCACTGGCAATTGATCGGGACATACGTGCCGAGTGCACCTGCGTGATCGAGTTCTCTGAGTCGCA
TCGATCCTCACCGGCAGTTCTCAGGACGGTCAACGCCCTTATCCCGATCACGTCGGGACAGGTACTCACCGCAGCCAACC
CCGACTCCTGGCCTTCGGGCGGGCTCACCGGGACGATCGTGCACCAATCGGCGGAATCGGAAGCTAAGTGTATCGTGAAA
ATTGCGCGAGCCATCCTCGGCAGGGCTCCCGGGCATCGGATTGGAGTGTTATCCCGAACAGCGCCGCGTCGCCGATTCGT
TGACGCAGCCTTCGCCGCGGCAACAGATATACCTCACTACAGGTGGGATGACGGCGTGTTGGACACAGATACTGCCAAAC
TCGTAAAGGCAATGCTCGCGAGGTTTGATGTCGCCGCCTACACTGCCGCGCCCGACAAAGTAACTTTCCTTCGGATGGCG
GTTGACTTTGACTCGATCACTGATGTCGACACGCGCAAGAGCATGGCGGATGCTCTCGGTTGGGTACAAGATCTGCTGTC
TAAAGCGGTGAAGCCAGAGAGCATCCGTGGACGCATCAAGGTTGGCGATTCAGCGACCCTCATCACTAGCCCTGGAGTCC
ATCTACTGCGCGGACACGTCGGCAAAGGGCAACAGTTCGACTGGGTGATTGTCGTAGGCCTTGAAAAGGGCTCCATTCCG
TTCACTATGGCTGACACATCAGACGACGAGATCGCTGAGGAAGCACGAGTGCTTTCCGTGATGATCTCACGCGCTCGGCA
CGGCGTCATTCTTAGCCGAGCCAACTCGGTTCGGACGAACGGGGGGCACCCCATGAGTCGAACTCCATCCCAGTTCCTCG
AATGGATTGCCACTGCCAATCCACTCGATGCGACTGGGATCATCGAGTGGTTCAAAGCTGCGGACTGGCGTGTAATCAGT
GAGAGATGA

Upstream 100 bases:

>100_bases
CAAAGTAAATGCCGCGCTATCAGTCCTGCCGCTCTTCACGGCGGCGAATGCCACGCAGGTGTCGTCTATCGAGAGTCTAC
TCGAAGAGATCGTGGCGTAG

Downstream 100 bases:

>100_bases
CCAACGTTCTTTGGAGAGAAGTGCGCTGTCCTTGATGATGATCCACCTCCGACCCGCGCAACCTTGGGCGCCGGACTCAC
CAGGTTGCAGATCGGATCAG

Product: putative ATP-dependent DNA helicase PcrA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 562; Mature: 562

Protein sequence:

>562_residues
MVSPTQKQIDIRDTRVLDLLVVAPAGCGKTEALALRIQGLLHRGDVTAPKKILVTTFSNRARDNIRERLGSYLTPSVMRD
RVTVVNFHGLAARLIRAHGNVIGLSEELRLPENDWVGEQLRQRNTSWNDRGAIQNALRETKQQPFSDAAVEAELERLGNV
TALELERLRKEEGRLTYDDLPRLAELILGYESVADLYATHFGAVVVDEFQDLTPQQLRIVNRIGYKRTTYGGDLVQGIYG
FAGAQPLAIDRDIRAECTCVIEFSESHRSSPAVLRTVNALIPITSGQVLTAANPDSWPSGGLTGTIVHQSAESEAKCIVK
IARAILGRAPGHRIGVLSRTAPRRRFVDAAFAAATDIPHYRWDDGVLDTDTAKLVKAMLARFDVAAYTAAPDKVTFLRMA
VDFDSITDVDTRKSMADALGWVQDLLSKAVKPESIRGRIKVGDSATLITSPGVHLLRGHVGKGQQFDWVIVVGLEKGSIP
FTMADTSDDEIAEEARVLSVMISRARHGVILSRANSVRTNGGHPMSRTPSQFLEWIATANPLDATGIIEWFKAADWRVIS
ER

Sequences:

>Translated_562_residues
MVSPTQKQIDIRDTRVLDLLVVAPAGCGKTEALALRIQGLLHRGDVTAPKKILVTTFSNRARDNIRERLGSYLTPSVMRD
RVTVVNFHGLAARLIRAHGNVIGLSEELRLPENDWVGEQLRQRNTSWNDRGAIQNALRETKQQPFSDAAVEAELERLGNV
TALELERLRKEEGRLTYDDLPRLAELILGYESVADLYATHFGAVVVDEFQDLTPQQLRIVNRIGYKRTTYGGDLVQGIYG
FAGAQPLAIDRDIRAECTCVIEFSESHRSSPAVLRTVNALIPITSGQVLTAANPDSWPSGGLTGTIVHQSAESEAKCIVK
IARAILGRAPGHRIGVLSRTAPRRRFVDAAFAAATDIPHYRWDDGVLDTDTAKLVKAMLARFDVAAYTAAPDKVTFLRMA
VDFDSITDVDTRKSMADALGWVQDLLSKAVKPESIRGRIKVGDSATLITSPGVHLLRGHVGKGQQFDWVIVVGLEKGSIP
FTMADTSDDEIAEEARVLSVMISRARHGVILSRANSVRTNGGHPMSRTPSQFLEWIATANPLDATGIIEWFKAADWRVIS
ER
>Mature_562_residues
MVSPTQKQIDIRDTRVLDLLVVAPAGCGKTEALALRIQGLLHRGDVTAPKKILVTTFSNRARDNIRERLGSYLTPSVMRD
RVTVVNFHGLAARLIRAHGNVIGLSEELRLPENDWVGEQLRQRNTSWNDRGAIQNALRETKQQPFSDAAVEAELERLGNV
TALELERLRKEEGRLTYDDLPRLAELILGYESVADLYATHFGAVVVDEFQDLTPQQLRIVNRIGYKRTTYGGDLVQGIYG
FAGAQPLAIDRDIRAECTCVIEFSESHRSSPAVLRTVNALIPITSGQVLTAANPDSWPSGGLTGTIVHQSAESEAKCIVK
IARAILGRAPGHRIGVLSRTAPRRRFVDAAFAAATDIPHYRWDDGVLDTDTAKLVKAMLARFDVAAYTAAPDKVTFLRMA
VDFDSITDVDTRKSMADALGWVQDLLSKAVKPESIRGRIKVGDSATLITSPGVHLLRGHVGKGQQFDWVIVVGLEKGSIP
FTMADTSDDEIAEEARVLSVMISRARHGVILSRANSVRTNGGHPMSRTPSQFLEWIATANPLDATGIIEWFKAADWRVIS
ER

Specific function: Essential helicase [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=303, Percent_Identity=29.042904290429, Blast_Score=83, Evalue=4e-17,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 61818; Mature: 61818

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVSPTQKQIDIRDTRVLDLLVVAPAGCGKTEALALRIQGLLHRGDVTAPKKILVTTFSNR
CCCCCCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHH
ARDNIRERLGSYLTPSVMRDRVTVVNFHGLAARLIRAHGNVIGLSEELRLPENDWVGEQL
HHHHHHHHHHHHCCHHHHHCCEEEEEHHHHHHHHHHHCCCEEECHHHCCCCCCCCHHHHH
RQRNTSWNDRGAIQNALRETKQQPFSDAAVEAELERLGNVTALELERLRKEEGRLTYDDL
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
PRLAELILGYESVADLYATHFGAVVVDEFQDLTPQQLRIVNRIGYKRTTYGGDLVQGIYG
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
FAGAQPLAIDRDIRAECTCVIEFSESHRSSPAVLRTVNALIPITSGQVLTAANPDSWPSG
HCCCCCEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHEEECCCCEEEECCCCCCCCC
GLTGTIVHQSAESEAKCIVKIARAILGRAPGHRIGVLSRTAPRRRFVDAAFAAATDIPHY
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
RWDDGVLDTDTAKLVKAMLARFDVAAYTAAPDKVTFLRMAVDFDSITDVDTRKSMADALG
CCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
WVQDLLSKAVKPESIRGRIKVGDSATLITSPGVHLLRGHVGKGQQFDWVIVVGLEKGSIP
HHHHHHHHCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCCCEEEEEEECCCCCC
FTMADTSDDEIAEEARVLSVMISRARHGVILSRANSVRTNGGHPMSRTPSQFLEWIATAN
EEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCC
PLDATGIIEWFKAADWRVISER
CCCHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure
MVSPTQKQIDIRDTRVLDLLVVAPAGCGKTEALALRIQGLLHRGDVTAPKKILVTTFSNR
CCCCCCCCCCCCHHHEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEEECCHH
ARDNIRERLGSYLTPSVMRDRVTVVNFHGLAARLIRAHGNVIGLSEELRLPENDWVGEQL
HHHHHHHHHHHHCCHHHHHCCEEEEEHHHHHHHHHHHCCCEEECHHHCCCCCCCCHHHHH
RQRNTSWNDRGAIQNALRETKQQPFSDAAVEAELERLGNVTALELERLRKEEGRLTYDDL
HHCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCHHHH
PRLAELILGYESVADLYATHFGAVVVDEFQDLTPQQLRIVNRIGYKRTTYGGDLVQGIYG
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCHHHHHHHHHHCCCCCCCCHHHHHHHHH
FAGAQPLAIDRDIRAECTCVIEFSESHRSSPAVLRTVNALIPITSGQVLTAANPDSWPSG
HCCCCCEEECCCCCCEEEEEEEECCCCCCCCHHHHHHHHHEEECCCCEEEECCCCCCCCC
GLTGTIVHQSAESEAKCIVKIARAILGRAPGHRIGVLSRTAPRRRFVDAAFAAATDIPHY
CCEEEEEECCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHHHCCCCC
RWDDGVLDTDTAKLVKAMLARFDVAAYTAAPDKVTFLRMAVDFDSITDVDTRKSMADALG
CCCCCCCCCCHHHHHHHHHHHHHHHHEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHH
WVQDLLSKAVKPESIRGRIKVGDSATLITSPGVHLLRGHVGKGQQFDWVIVVGLEKGSIP
HHHHHHHHCCCCCCCCCEEEECCCEEEEECCCHHHHHCCCCCCCCCCEEEEEEECCCCCC
FTMADTSDDEIAEEARVLSVMISRARHGVILSRANSVRTNGGHPMSRTPSQFLEWIATAN
EEECCCCCHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCC
PLDATGIIEWFKAADWRVISER
CCCHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA