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The map label for this gene is bmr3 [H]

Identifier: 50842318

GI number: 50842318

Start: 911934

End: 913697

Strand: Direct

Name: bmr3 [H]

Synonym: PPA0832

Alternate gene names: 50842318

Gene position: 911934-913697 (Clockwise)

Preceding gene: 50842317

Following gene: 50842319

Centisome position: 35.62

GC content: 60.15

Gene sequence:

>1764_bases
ATGACCGCAACTGCTGCTGCCGCTGTCAAGTTCAGACCGGACCGTCGGTTCTGGGTTGTCTACTTCTGTCTCATGATGGT
GATGTTCCTGTCCTCCTTGGACCAGACGATCGTCGCCACGGCTCTTCCGACCATCGTCGGTGACCTGTCCGGTGTCGAGC
ACATGGCATGGGTCATCACCGCTTATACGCTTGCTATCACGGTAGGCATGCCGCTATACGGCCGGTTGTCCGACCTTATT
GGCCGCAAGACGCTTTATCTCATCGCCATTGGTCTGTTTCTGTTGGGGTCTGCCCTCTGTGGTACCGCCGGCGCCATGAT
CACGTTCATTTTCTACCGCTTCATTCAGGGCCTGGGTGGTGGTGGCCTCATGATCTTGTCCCAGGCGATCACCGGAGACC
TCATTCCGCCGCGCGTCCGCGCTGCTTACATGGCGCCGATGGGTGCGATGTTCGGCGTCTCCTCGGTGCTTGGGCCGCTT
CTGGGCGGCTGGCTCACCGACTCGATCTCCTGGCGCTGGGTGTTCTGGATCAACCTGCCACTTGGCGTGGTGGCCTGGAT
CGCCTGCCTCTTTGCTCTTAAACTCCCCAAGCACGAACTGGCGGCCAAGATCGACTGGCTTGGACTGACTCTCATGGACG
CCGGTGCCGTGGCGATCGTGCTGCTCGCCACCTGGGGCGGTTCCCAGTACGACTGGTTGTCGTGGCAAATCATCGGTCTG
GGCGTCATCACGGTCGTAGCATGGGGTCTGCTGCCCTTTGTGGAGCGTCGCATCGAGGATCCGGTGTTGCCGCTCGAGCT
CTTCCGCAATCGCACTTTCGTCGTCTCGACGATTATCGGCATGCTGGCCATGGGTGCGATGTTCGGTGTGCTTGCTTACC
TGCCGACCTATATGCAGATGACCTATGGCTACTCGGCTACCGTGTCTGGCCTGCTGCTCATCCCCATGACGATCGGGATT
CTGCTGGCCTCGACGGTGACTGGTCTGCTTACCTCTCGCATCGGTCGGTACCGTATCTTCATCATCATTGGTCCGATTGT
CGCGGCGGGTGGCATGGTGCTGATGTCCACCCTCAACCAGAACTCGCCAGTGTGGCGCATTATGGGTGATACCTTCGTCC
TCGGTTTGGGCATGGGTATGTTCTTCCAGCTGCTCGTCATGGCGGTGCAAAACGACGTCCGCCCAACCTTGCTTGGAACC
GCTACCAGCCACAACAACTTCTTCCGCCAGATTGGTGTGTGCCTGGGTGCCTCTCTTATTGGTGTGGCTTTCACCACCCG
CCTGACCAACCGCGTCACCGATCTGTTCACGTCCTTGGCCGCGAGCAAGAACCCCGCGGTCCTCAAGGCGCTGGGGGCCA
TGAGCTCGCACCATGCTGCGGCCTCGCTGACTCCGGGGGCCGTCAATCAGATGCCCGATCTCATTCGTAACGGCATCATC
GAGGCATACGTCAACTCTTTAACCCCGCTGTTTTTGTGGATGGCGCCGATGGTTGCAGCTGCGGGTTTGTTGGCCTTCTT
GCTCAAGGATGTGCCGCTGTCCCACCACACCGGTTTGCAGATGCGTGCCGCGTCCGAGGCTGAGATGGAAGGTGCCCAGG
AAGTTCCGGCAGTCGAGGGTCGCGAAAACCTGTCAGAAAACGGGCACGGTGACTTTTCTGACGACCTGGCCGGGTCGTCG
GCTCGTCGCGCGACAGATGTTACGAAGGTCAAACCCGCCCCGCCGAGGCACTCCGCCGGATCGAACCAACCGGGACAGGC
CTGA

Upstream 100 bases:

>100_bases
CCATGAGCCGAGCCCTGGCCATCTAGACCTGACGGCCCTCATCGTGCGCGCGTTCGAACGCGCCATTCCATCTGTCTCAG
ATCGTTACGAAAGTTGTTAC

Downstream 100 bases:

>100_bases
GAGCTCGAGACGTATCACGGGGGCCGGGTGACGCGTCCTAGCGTCTAAACTGGTTATCTGTCCTGGGTCGGAAGGCCCGA
TTCCATTTTCTGAGGAGAGA

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter 3 [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MTATAAAAVKFRPDRRFWVVYFCLMMVMFLSSLDQTIVATALPTIVGDLSGVEHMAWVITAYTLAITVGMPLYGRLSDLI
GRKTLYLIAIGLFLLGSALCGTAGAMITFIFYRFIQGLGGGGLMILSQAITGDLIPPRVRAAYMAPMGAMFGVSSVLGPL
LGGWLTDSISWRWVFWINLPLGVVAWIACLFALKLPKHELAAKIDWLGLTLMDAGAVAIVLLATWGGSQYDWLSWQIIGL
GVITVVAWGLLPFVERRIEDPVLPLELFRNRTFVVSTIIGMLAMGAMFGVLAYLPTYMQMTYGYSATVSGLLLIPMTIGI
LLASTVTGLLTSRIGRYRIFIIIGPIVAAGGMVLMSTLNQNSPVWRIMGDTFVLGLGMGMFFQLLVMAVQNDVRPTLLGT
ATSHNNFFRQIGVCLGASLIGVAFTTRLTNRVTDLFTSLAASKNPAVLKALGAMSSHHAAASLTPGAVNQMPDLIRNGII
EAYVNSLTPLFLWMAPMVAAAGLLAFLLKDVPLSHHTGLQMRAASEAEMEGAQEVPAVEGRENLSENGHGDFSDDLAGSS
ARRATDVTKVKPAPPRHSAGSNQPGQA

Sequences:

>Translated_587_residues
MTATAAAAVKFRPDRRFWVVYFCLMMVMFLSSLDQTIVATALPTIVGDLSGVEHMAWVITAYTLAITVGMPLYGRLSDLI
GRKTLYLIAIGLFLLGSALCGTAGAMITFIFYRFIQGLGGGGLMILSQAITGDLIPPRVRAAYMAPMGAMFGVSSVLGPL
LGGWLTDSISWRWVFWINLPLGVVAWIACLFALKLPKHELAAKIDWLGLTLMDAGAVAIVLLATWGGSQYDWLSWQIIGL
GVITVVAWGLLPFVERRIEDPVLPLELFRNRTFVVSTIIGMLAMGAMFGVLAYLPTYMQMTYGYSATVSGLLLIPMTIGI
LLASTVTGLLTSRIGRYRIFIIIGPIVAAGGMVLMSTLNQNSPVWRIMGDTFVLGLGMGMFFQLLVMAVQNDVRPTLLGT
ATSHNNFFRQIGVCLGASLIGVAFTTRLTNRVTDLFTSLAASKNPAVLKALGAMSSHHAAASLTPGAVNQMPDLIRNGII
EAYVNSLTPLFLWMAPMVAAAGLLAFLLKDVPLSHHTGLQMRAASEAEMEGAQEVPAVEGRENLSENGHGDFSDDLAGSS
ARRATDVTKVKPAPPRHSAGSNQPGQA
>Mature_586_residues
TATAAAAVKFRPDRRFWVVYFCLMMVMFLSSLDQTIVATALPTIVGDLSGVEHMAWVITAYTLAITVGMPLYGRLSDLIG
RKTLYLIAIGLFLLGSALCGTAGAMITFIFYRFIQGLGGGGLMILSQAITGDLIPPRVRAAYMAPMGAMFGVSSVLGPLL
GGWLTDSISWRWVFWINLPLGVVAWIACLFALKLPKHELAAKIDWLGLTLMDAGAVAIVLLATWGGSQYDWLSWQIIGLG
VITVVAWGLLPFVERRIEDPVLPLELFRNRTFVVSTIIGMLAMGAMFGVLAYLPTYMQMTYGYSATVSGLLLIPMTIGIL
LASTVTGLLTSRIGRYRIFIIIGPIVAAGGMVLMSTLNQNSPVWRIMGDTFVLGLGMGMFFQLLVMAVQNDVRPTLLGTA
TSHNNFFRQIGVCLGASLIGVAFTTRLTNRVTDLFTSLAASKNPAVLKALGAMSSHHAAASLTPGAVNQMPDLIRNGIIE
AYVNSLTPLFLWMAPMVAAAGLLAFLLKDVPLSHHTGLQMRAASEAEMEGAQEVPAVEGRENLSENGHGDFSDDLAGSSA
RRATDVTKVKPAPPRHSAGSNQPGQA

Specific function: Confers resistance to puromycin, tosufloxacin and norfloxacin [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=463, Percent_Identity=30.0215982721382, Blast_Score=174, Evalue=1e-44,
Organism=Escherichia coli, GI1788710, Length=478, Percent_Identity=28.6610878661088, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1790195, Length=402, Percent_Identity=28.6069651741293, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081983, Length=358, Percent_Identity=28.4916201117318, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788392, Length=339, Percent_Identity=32.7433628318584, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1790146, Length=193, Percent_Identity=28.4974093264249, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=472, Percent_Identity=26.0593220338983, Blast_Score=157, Evalue=4e-39,
Organism=Saccharomyces cerevisiae, GI6323735, Length=449, Percent_Identity=27.3942093541203, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6325455, Length=450, Percent_Identity=24.2222222222222, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319770, Length=479, Percent_Identity=26.9311064718163, Blast_Score=116, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319783, Length=371, Percent_Identity=25.3369272237197, Blast_Score=114, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6321663, Length=463, Percent_Identity=24.1900647948164, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6324954, Length=454, Percent_Identity=23.3480176211454, Blast_Score=64, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 62935; Mature: 62804

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTATAAAAVKFRPDRRFWVVYFCLMMVMFLSSLDQTIVATALPTIVGDLSGVEHMAWVIT
CCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AYTLAITVGMPLYGRLSDLIGRKTLYLIAIGLFLLGSALCGTAGAMITFIFYRFIQGLGG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGLMILSQAITGDLIPPRVRAAYMAPMGAMFGVSSVLGPLLGGWLTDSISWRWVFWINLP
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECH
LGVVAWIACLFALKLPKHELAAKIDWLGLTLMDAGAVAIVLLATWGGSQYDWLSWQIIGL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCCEEEEHHHH
GVITVVAWGLLPFVERRIEDPVLPLELFRNRTFVVSTIIGMLAMGAMFGVLAYLPTYMQM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYGYSATVSGLLLIPMTIGILLASTVTGLLTSRIGRYRIFIIIGPIVAAGGMVLMSTLNQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHCHHHHHHHHCC
NSPVWRIMGDTFVLGLGMGMFFQLLVMAVQNDVRPTLLGTATSHNNFFRQIGVCLGASLI
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
GVAFTTRLTNRVTDLFTSLAASKNPAVLKALGAMSSHHAAASLTPGAVNQMPDLIRNGII
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EAYVNSLTPLFLWMAPMVAAAGLLAFLLKDVPLSHHTGLQMRAASEAEMEGAQEVPAVEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHCCHHCCCCCCC
RENLSENGHGDFSDDLAGSSARRATDVTKVKPAPPRHSAGSNQPGQA
CHHCCCCCCCCCCHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TATAAAAVKFRPDRRFWVVYFCLMMVMFLSSLDQTIVATALPTIVGDLSGVEHMAWVIT
CCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
AYTLAITVGMPLYGRLSDLIGRKTLYLIAIGLFLLGSALCGTAGAMITFIFYRFIQGLGG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
GGLMILSQAITGDLIPPRVRAAYMAPMGAMFGVSSVLGPLLGGWLTDSISWRWVFWINLP
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECH
LGVVAWIACLFALKLPKHELAAKIDWLGLTLMDAGAVAIVLLATWGGSQYDWLSWQIIGL
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCCEEEEHHHH
GVITVVAWGLLPFVERRIEDPVLPLELFRNRTFVVSTIIGMLAMGAMFGVLAYLPTYMQM
HHHHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TYGYSATVSGLLLIPMTIGILLASTVTGLLTSRIGRYRIFIIIGPIVAAGGMVLMSTLNQ
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECHHHHCHHHHHHHHCC
NSPVWRIMGDTFVLGLGMGMFFQLLVMAVQNDVRPTLLGTATSHNNFFRQIGVCLGASLI
CCCEEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHH
GVAFTTRLTNRVTDLFTSLAASKNPAVLKALGAMSSHHAAASLTPGAVNQMPDLIRNGII
HHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHH
EAYVNSLTPLFLWMAPMVAAAGLLAFLLKDVPLSHHTGLQMRAASEAEMEGAQEVPAVEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCHHHHCCHHCCCCCCC
RENLSENGHGDFSDDLAGSSARRATDVTKVKPAPPRHSAGSNQPGQA
CHHCCCCCCCCCCHHHCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9023234; 9384377 [H]