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The map label for this gene is pyk [H]

Identifier: 50842256

GI number: 50842256

Start: 843812

End: 845245

Strand: Direct

Name: pyk [H]

Synonym: PPA0769

Alternate gene names: 50842256

Gene position: 843812-845245 (Clockwise)

Preceding gene: 50842255

Following gene: 50842257

Centisome position: 32.96

GC content: 59.76

Gene sequence:

>1434_bases
GTGCGTAGAGCTAAGATTGTGAATACCCTTGGGCCCGCTGTGACCAGCCATGACGCCATGAAGGAGCTCATGGAGGCTGG
CATGAATATTGCCCGTCTCAACATGAGCCACGGCGACTACTCCGAGCACCAGGAGCGTCTTGACCTGGTTCGCTCGGTCT
CGAAAGAGCTTGGTCTCAACGTGGCAGCCTTGGCCGACCTCCAAGGCCCGAAGATCCGTACCGGCCTGTTCGAGAAGGCC
GAAGGAGAGTCGAACGGCAAGATCGACCTCAAGATTGGTGACAAGTTCACTATCACCACTGACGACATTGTGGGCAATCA
GGAGCGCGTCTCCACCACTTTCAAGGGTCTGCCCCAGGACTGCAAGCCCGGCGACGTCATCCTCATCGACGACGGCAAGA
CCGTCCTCCAGGTTGACTCTGTGAGCGGCAACGACGTCAATTGTCACTGCACTGTCGCTGGCCCGGTTGGCGACCACAAG
GGCATCAACCTCCCCGGTGTTGCCGTGTCTATTCCCGCCCTCACCAAAAAGGACGAGGAGAACCTGCGCTGGGCTCTCAA
AGCTGGCATCGACCTTGTTGCTCTGTCCTTCGTCCGACACGGCTCTGACATCGACCGTGTCCACGAGATTATGGACGAGG
AGGGACGTACCGTCCCCGTTATAGCCAAGCTCGAGAAGCCGCAGGCTATCGAGAACCTGGACGAGATTATTGACGTCTTT
GACGCCGTCATGGTTGCTCGTGGCGATATGGCCGTCGAGTGCCCGCTCGAGGAGGTTCCGCTGATCCAAAAGCAGATCAT
CGAGAAGGCTCGGTTACAGGCTAAGCCCGTCATCGTGGCCACCCAGATGCTCGAGTCGATGATCCACGCTCCCCGCCCGA
CCCGCGCTGAGGCCGCCGACGTCGCCAACGCCATCCTTGACGGCGCCGACGGCGTCATGACGTCCGCTGAGACCAGCGTT
GGTGACTTCCCAGGTGAGACTGTCCGCACCATGGCCAAGATCGTTGAGTCTACTGAGGCCCGTGGCTTGGACAAGATCGC
CAAGATCGACTGGGATCCGCACACCACCAGTGGCATCATGTCGAAGGCAGCTGCTGAGATCGCTGAGCGCGCCGAGGCCA
AGTTCATCGTGGCCTTTACCAAGTCCGGTGACACCGCCCGTCGTATCGCTCGTCTGCGTCCGAGCACCCCGCTCATCGTT
TTCACCTCTGATGAGACCACGACCAAGACCCTCGCCTGGGTCTGGGGCGCTCACGCCGTCGTTACCCCGGTGTTTAAGAA
TGCGGAGGAGCTGTACCGCTGGGTTAACGCGTACCTGCGCGACAAGGGTTTCGTGCCGGTCGGTGACCGCGTCGTCGTGT
TGTCCGGTTCCCCGATGGATATTCCGGGTAAGACCAACAATCTGCGCATCCTTCGTATCAAGGAAGATGACTGA

Upstream 100 bases:

>100_bases
GGTGGTCATTTGTGCACTATAGGTGAACGTCTAGTGTACATCCGACGGTGACTGGCATTCCGGCTCGGGCCCGCGTTGCC
TTGAGCGGTATCGTGTAGGC

Downstream 100 bases:

>100_bases
TCGCGGGTATGCCTCGATCGTTACGACGTCCCTCATTCCGTGAGGGACGTCGTCGCTTTCCTGGACGGCCCATCCCGAAG
CGGCCTAGCGTTTCCGAGGA

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MRRAKIVNTLGPAVTSHDAMKELMEAGMNIARLNMSHGDYSEHQERLDLVRSVSKELGLNVAALADLQGPKIRTGLFEKA
EGESNGKIDLKIGDKFTITTDDIVGNQERVSTTFKGLPQDCKPGDVILIDDGKTVLQVDSVSGNDVNCHCTVAGPVGDHK
GINLPGVAVSIPALTKKDEENLRWALKAGIDLVALSFVRHGSDIDRVHEIMDEEGRTVPVIAKLEKPQAIENLDEIIDVF
DAVMVARGDMAVECPLEEVPLIQKQIIEKARLQAKPVIVATQMLESMIHAPRPTRAEAADVANAILDGADGVMTSAETSV
GDFPGETVRTMAKIVESTEARGLDKIAKIDWDPHTTSGIMSKAAAEIAERAEAKFIVAFTKSGDTARRIARLRPSTPLIV
FTSDETTTKTLAWVWGAHAVVTPVFKNAEELYRWVNAYLRDKGFVPVGDRVVVLSGSPMDIPGKTNNLRILRIKEDD

Sequences:

>Translated_477_residues
MRRAKIVNTLGPAVTSHDAMKELMEAGMNIARLNMSHGDYSEHQERLDLVRSVSKELGLNVAALADLQGPKIRTGLFEKA
EGESNGKIDLKIGDKFTITTDDIVGNQERVSTTFKGLPQDCKPGDVILIDDGKTVLQVDSVSGNDVNCHCTVAGPVGDHK
GINLPGVAVSIPALTKKDEENLRWALKAGIDLVALSFVRHGSDIDRVHEIMDEEGRTVPVIAKLEKPQAIENLDEIIDVF
DAVMVARGDMAVECPLEEVPLIQKQIIEKARLQAKPVIVATQMLESMIHAPRPTRAEAADVANAILDGADGVMTSAETSV
GDFPGETVRTMAKIVESTEARGLDKIAKIDWDPHTTSGIMSKAAAEIAERAEAKFIVAFTKSGDTARRIARLRPSTPLIV
FTSDETTTKTLAWVWGAHAVVTPVFKNAEELYRWVNAYLRDKGFVPVGDRVVVLSGSPMDIPGKTNNLRILRIKEDD
>Mature_477_residues
MRRAKIVNTLGPAVTSHDAMKELMEAGMNIARLNMSHGDYSEHQERLDLVRSVSKELGLNVAALADLQGPKIRTGLFEKA
EGESNGKIDLKIGDKFTITTDDIVGNQERVSTTFKGLPQDCKPGDVILIDDGKTVLQVDSVSGNDVNCHCTVAGPVGDHK
GINLPGVAVSIPALTKKDEENLRWALKAGIDLVALSFVRHGSDIDRVHEIMDEEGRTVPVIAKLEKPQAIENLDEIIDVF
DAVMVARGDMAVECPLEEVPLIQKQIIEKARLQAKPVIVATQMLESMIHAPRPTRAEAADVANAILDGADGVMTSAETSV
GDFPGETVRTMAKIVESTEARGLDKIAKIDWDPHTTSGIMSKAAAEIAERAEAKFIVAFTKSGDTARRIARLRPSTPLIV
FTSDETTTKTLAWVWGAHAVVTPVFKNAEELYRWVNAYLRDKGFVPVGDRVVVLSGSPMDIPGKTNNLRILRIKEDD

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=494, Percent_Identity=36.4372469635627, Blast_Score=298, Evalue=9e-81,
Organism=Homo sapiens, GI33286422, Length=491, Percent_Identity=35.8452138492872, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI33286420, Length=491, Percent_Identity=35.8452138492872, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI32967597, Length=496, Percent_Identity=35.8870967741936, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI10835121, Length=496, Percent_Identity=35.8870967741936, Blast_Score=281, Evalue=8e-76,
Organism=Homo sapiens, GI310128732, Length=290, Percent_Identity=34.8275862068965, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI310128730, Length=290, Percent_Identity=34.8275862068965, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI310128736, Length=238, Percent_Identity=32.3529411764706, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI310128734, Length=238, Percent_Identity=32.3529411764706, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI310128738, Length=217, Percent_Identity=31.3364055299539, Blast_Score=125, Evalue=8e-29,
Organism=Escherichia coli, GI1787965, Length=471, Percent_Identity=39.4904458598726, Blast_Score=340, Evalue=9e-95,
Organism=Escherichia coli, GI1788160, Length=492, Percent_Identity=40.0406504065041, Blast_Score=317, Evalue=9e-88,
Organism=Caenorhabditis elegans, GI17544584, Length=488, Percent_Identity=36.4754098360656, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17506829, Length=491, Percent_Identity=36.4562118126273, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71984413, Length=491, Percent_Identity=36.4562118126273, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71984406, Length=491, Percent_Identity=36.4562118126273, Blast_Score=271, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI17506831, Length=491, Percent_Identity=36.4562118126273, Blast_Score=270, Evalue=1e-72,
Organism=Saccharomyces cerevisiae, GI6319279, Length=415, Percent_Identity=38.7951807228916, Blast_Score=278, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6324923, Length=498, Percent_Identity=35.5421686746988, Blast_Score=268, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24648964, Length=505, Percent_Identity=35.6435643564356, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI28571814, Length=505, Percent_Identity=35.8415841584158, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24648966, Length=396, Percent_Identity=37.3737373737374, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24581235, Length=502, Percent_Identity=30.0796812749004, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI24646914, Length=307, Percent_Identity=34.2019543973941, Blast_Score=162, Evalue=4e-40,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51860; Mature: 51860

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRAKIVNTLGPAVTSHDAMKELMEAGMNIARLNMSHGDYSEHQERLDLVRSVSKELGLN
CCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
VAALADLQGPKIRTGLFEKAEGESNGKIDLKIGDKFTITTDDIVGNQERVSTTFKGLPQD
EEEEECCCCCCCHHCCHHHCCCCCCCEEEEEECCEEEEECHHHCCCHHHHHHHHHCCCCC
CKPGDVILIDDGKTVLQVDSVSGNDVNCHCTVAGPVGDHKGINLPGVAVSIPALTKKDEE
CCCCCEEEECCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHH
NLRWALKAGIDLVALSFVRHGSDIDRVHEIMDEEGRTVPVIAKLEKPQAIENLDEIIDVF
HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
DAVMVARGDMAVECPLEEVPLIQKQIIEKARLQAKPVIVATQMLESMIHAPRPTRAEAAD
HHHHHHCCCEEEECCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
VANAILDGADGVMTSAETSVGDFPGETVRTMAKIVESTEARGLDKIAKIDWDPHTTSGIM
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
SKAAAEIAERAEAKFIVAFTKSGDTARRIARLRPSTPLIVFTSDETTTKTLAWVWGAHAV
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCHHH
VTPVFKNAEELYRWVNAYLRDKGFVPVGDRVVVLSGSPMDIPGKTNNLRILRIKEDD
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCC
>Mature Secondary Structure
MRRAKIVNTLGPAVTSHDAMKELMEAGMNIARLNMSHGDYSEHQERLDLVRSVSKELGLN
CCCHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCC
VAALADLQGPKIRTGLFEKAEGESNGKIDLKIGDKFTITTDDIVGNQERVSTTFKGLPQD
EEEEECCCCCCCHHCCHHHCCCCCCCEEEEEECCEEEEECHHHCCCHHHHHHHHHCCCCC
CKPGDVILIDDGKTVLQVDSVSGNDVNCHCTVAGPVGDHKGINLPGVAVSIPALTKKDEE
CCCCCEEEECCCCEEEEEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEECCCCCCCCHH
NLRWALKAGIDLVALSFVRHGSDIDRVHEIMDEEGRTVPVIAKLEKPQAIENLDEIIDVF
HHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHH
DAVMVARGDMAVECPLEEVPLIQKQIIEKARLQAKPVIVATQMLESMIHAPRPTRAEAAD
HHHHHHCCCEEEECCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHH
VANAILDGADGVMTSAETSVGDFPGETVRTMAKIVESTEARGLDKIAKIDWDPHTTSGIM
HHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHCCCCCCCHHHHH
SKAAAEIAERAEAKFIVAFTKSGDTARRIARLRPSTPLIVFTSDETTTKTLAWVWGAHAV
HHHHHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHCCHHH
VTPVFKNAEELYRWVNAYLRDKGFVPVGDRVVVLSGSPMDIPGKTNNLRILRIKEDD
HHHHHCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8074528; 12948626 [H]