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The map label for this gene is glnA [H]

Identifier: 50842151

GI number: 50842151

Start: 728895

End: 730301

Strand: Direct

Name: glnA [H]

Synonym: PPA0664

Alternate gene names: 50842151

Gene position: 728895-730301 (Clockwise)

Preceding gene: 50842146

Following gene: 50842153

Centisome position: 28.47

GC content: 56.86

Gene sequence:

>1407_bases
ATGTCAGCAGTCTACCGAAGCCATGACCAACCTTGTTACCAGCTCGAAACACATAACGGGCACACTGGAAAGGTGAGCAG
GCAGACTGAATTCGTCCTGCGTTCTATGGAGGAACGCAACATCCGTTTCGTAAGGCTGTGGTTCACGGACGTGCTGGGAT
CCCTGAAATCTGTTGCCATCGCCCCGGCTGAAGTTGAGGGAGCTTTCGTTGAAGGCGTGGGCTTCGACGGCTCAGCTATC
GAGGGGTATGCCCGCGTCTACGAGGCAGATATGGTGGCCCACCCCGATCCGGCCACCTTCCAAGTTCTGCCATGGCGTTC
TGGCACCAGCACGGCCAGGATGTTCTGCGATATCACCAATCCTGACGGAACCTCCTCTGCAGCTGATCCTCGTCACGTCC
TCAAGAGGACGATGGGTCGTGCCGCAGAAATGGGTTTTACCTTCTATGTTCACCCAGAGATTGAGTTCTACCTGCTTAAC
GATGAGCATCATCCTGGGGAATCCCCGGTGGCGCTGGACAACGGTGGCTACTTCGACCACACCACCTTGGGAGCGGGCAC
CGACTTCCGTCGCGACGCCATCAACGTCCTGGAACAGATGGGTATTTCGGTAGAGTTCAGCCACCACGAGGCCGCTCCTG
GACAGCACGAAATTGACCTTCGTTACGCCGATGCGCTGACGATGGCCGACAACATCATGACCTTCCGGGTAGTCATTCGG
GAGATCGCTTCCCTCAAGGGGATCAAGGCCACCTTCATGCCTAAGCCTTTTACCGATCACGCCGGGTCAGGTATGCATTC
TCACGTCTCCCTCTTTGAGGGGGACACCAATGCCTTCTATGACGCGGCTGACGAGGCTCGCATGTCGCCGGTTGCGAAGT
ATTTCGTCGCCGGTCTTTTGCACCATGCTCCGGAGATCATCGCCGTGACGAACCAGTGGGTTAACTCCTACCGTCGGCTT
TCCGGTGGGGGAGAGGCGCCTAACTACATTTGCTGGGGTCGCAATAATCGTTCAGCACTGGTTCGTATTCCGATGTATAA
GCCCGACAAGGCTTCCTCGGCTCGGGTGGAGTTGCGTTCTATCGATTCTGCTGCCAACCCATATCTGGCGTATTCCTTGG
TACTTGCTGCTGGTTTGGACGGCATCGAGAAGGAGCTACCGCTTCCAGAGGAGGCATCCGACGACGTGTGGCAGTTGTCA
GCTCGCGAGCGTCACGCACTGGGGATCAAGCAGCTGCCGCAAAGTCTGGGTGCTGCGATCCGCTGCATGGAGGAGTCCGA
GCTCGTGGCTGAGACCCTCGGCGAACACGTCTACGATTATTTCTTACGCAATAAGAGGGCGGAATTTGAGGAGTACAACC
GGCAGGTCAGCCAGTTCGAGCTTGATCGCTACCTGCCACACCTCTAA

Upstream 100 bases:

>100_bases
GGTGGTAGTGCGGTCGGTCCACCGCTCGCCGTCAAACCAGCGATAGCGATCCGGCGTGCCGTCAGGGTCTCGATACCACC
CCGCTTGAGGACCCGAAGCC

Downstream 100 bases:

>100_bases
ACAGGTTGGAGCTGGCGTCTAGGCGTCGGCAAAAATGTCGCGGAAAACTCCGGCCATATCAACGACTGGGAACACGCCAG
CAACGTCGTCGACGTGGGAC

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase 2 [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MSAVYRSHDQPCYQLETHNGHTGKVSRQTEFVLRSMEERNIRFVRLWFTDVLGSLKSVAIAPAEVEGAFVEGVGFDGSAI
EGYARVYEADMVAHPDPATFQVLPWRSGTSTARMFCDITNPDGTSSAADPRHVLKRTMGRAAEMGFTFYVHPEIEFYLLN
DEHHPGESPVALDNGGYFDHTTLGAGTDFRRDAINVLEQMGISVEFSHHEAAPGQHEIDLRYADALTMADNIMTFRVVIR
EIASLKGIKATFMPKPFTDHAGSGMHSHVSLFEGDTNAFYDAADEARMSPVAKYFVAGLLHHAPEIIAVTNQWVNSYRRL
SGGGEAPNYICWGRNNRSALVRIPMYKPDKASSARVELRSIDSAANPYLAYSLVLAAGLDGIEKELPLPEEASDDVWQLS
ARERHALGIKQLPQSLGAAIRCMEESELVAETLGEHVYDYFLRNKRAEFEEYNRQVSQFELDRYLPHL

Sequences:

>Translated_468_residues
MSAVYRSHDQPCYQLETHNGHTGKVSRQTEFVLRSMEERNIRFVRLWFTDVLGSLKSVAIAPAEVEGAFVEGVGFDGSAI
EGYARVYEADMVAHPDPATFQVLPWRSGTSTARMFCDITNPDGTSSAADPRHVLKRTMGRAAEMGFTFYVHPEIEFYLLN
DEHHPGESPVALDNGGYFDHTTLGAGTDFRRDAINVLEQMGISVEFSHHEAAPGQHEIDLRYADALTMADNIMTFRVVIR
EIASLKGIKATFMPKPFTDHAGSGMHSHVSLFEGDTNAFYDAADEARMSPVAKYFVAGLLHHAPEIIAVTNQWVNSYRRL
SGGGEAPNYICWGRNNRSALVRIPMYKPDKASSARVELRSIDSAANPYLAYSLVLAAGLDGIEKELPLPEEASDDVWQLS
ARERHALGIKQLPQSLGAAIRCMEESELVAETLGEHVYDYFLRNKRAEFEEYNRQVSQFELDRYLPHL
>Mature_467_residues
SAVYRSHDQPCYQLETHNGHTGKVSRQTEFVLRSMEERNIRFVRLWFTDVLGSLKSVAIAPAEVEGAFVEGVGFDGSAIE
GYARVYEADMVAHPDPATFQVLPWRSGTSTARMFCDITNPDGTSSAADPRHVLKRTMGRAAEMGFTFYVHPEIEFYLLND
EHHPGESPVALDNGGYFDHTTLGAGTDFRRDAINVLEQMGISVEFSHHEAAPGQHEIDLRYADALTMADNIMTFRVVIRE
IASLKGIKATFMPKPFTDHAGSGMHSHVSLFEGDTNAFYDAADEARMSPVAKYFVAGLLHHAPEIIAVTNQWVNSYRRLS
GGGEAPNYICWGRNNRSALVRIPMYKPDKASSARVELRSIDSAANPYLAYSLVLAAGLDGIEKELPLPEEASDDVWQLSA
RERHALGIKQLPQSLGAAIRCMEESELVAETLGEHVYDYFLRNKRAEFEEYNRQVSQFELDRYLPHL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=451, Percent_Identity=25.9423503325942, Blast_Score=124, Evalue=1e-28,
Organism=Escherichia coli, GI1790301, Length=437, Percent_Identity=36.1556064073227, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI87081870, Length=470, Percent_Identity=28.7234042553192, Blast_Score=149, Evalue=3e-37,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52296; Mature: 52165

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAVYRSHDQPCYQLETHNGHTGKVSRQTEFVLRSMEERNIRFVRLWFTDVLGSLKSVAI
CCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHEE
APAEVEGAFVEGVGFDGSAIEGYARVYEADMVAHPDPATFQVLPWRSGTSTARMFCDITN
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECC
PDGTSSAADPRHVLKRTMGRAAEMGFTFYVHPEIEFYLLNDEHHPGESPVALDNGGYFDH
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCEEECCCCCCCC
TTLGAGTDFRRDAINVLEQMGISVEFSHHEAAPGQHEIDLRYADALTMADNIMTFRVVIR
CCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
EIASLKGIKATFMPKPFTDHAGSGMHSHVSLFEGDTNAFYDAADEARMSPVAKYFVAGLL
HHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHH
HHAPEIIAVTNQWVNSYRRLSGGGEAPNYICWGRNNRSALVRIPMYKPDKASSARVELRS
HCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCEEEEEC
IDSAANPYLAYSLVLAAGLDGIEKELPLPEEASDDVWQLSARERHALGIKQLPQSLGAAI
CCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHCCHHHHHCCHHHHHHHHHHHH
RCMEESELVAETLGEHVYDYFLRNKRAEFEEYNRQVSQFELDRYLPHL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SAVYRSHDQPCYQLETHNGHTGKVSRQTEFVLRSMEERNIRFVRLWFTDVLGSLKSVAI
CCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHEE
APAEVEGAFVEGVGFDGSAIEGYARVYEADMVAHPDPATFQVLPWRSGTSTARMFCDITN
CCCCCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCEEEEEEECC
PDGTSSAADPRHVLKRTMGRAAEMGFTFYVHPEIEFYLLNDEHHPGESPVALDNGGYFDH
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEECCCEEEEEECCCCCCCCCCEEECCCCCCCC
TTLGAGTDFRRDAINVLEQMGISVEFSHHEAAPGQHEIDLRYADALTMADNIMTFRVVIR
CCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHH
EIASLKGIKATFMPKPFTDHAGSGMHSHVSLFEGDTNAFYDAADEARMSPVAKYFVAGLL
HHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHHHHH
HHAPEIIAVTNQWVNSYRRLSGGGEAPNYICWGRNNRSALVRIPMYKPDKASSARVELRS
HCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEECCCCCCEEEEECCCCCCCCCCCEEEEEC
IDSAANPYLAYSLVLAAGLDGIEKELPLPEEASDDVWQLSARERHALGIKQLPQSLGAAI
CCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCHHHHCCHHHHHCCHHHHHHHHHHHH
RCMEESELVAETLGEHVYDYFLRNKRAEFEEYNRQVSQFELDRYLPHL
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA