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The map label for this gene is purR [H]

Identifier: 50842089

GI number: 50842089

Start: 666300

End: 667298

Strand: Reverse

Name: purR [H]

Synonym: PPA0603

Alternate gene names: 50842089

Gene position: 667298-666300 (Counterclockwise)

Preceding gene: 50842098

Following gene: 50842088

Centisome position: 26.06

GC content: 62.06

Gene sequence:

>999_bases
ATGTCATCAAGAGCAACCCTCAGCGACGTCGCCAAAGCATGCGGCGTCTCCTCGCAAACCGTCTCCCGGGCGCTCAACGA
CAGTCCACTCGTCCGACCTGAGACCAGAGCCAAGGTCATGGCCGCCGTCGAGACCGTCGGATATGAACCAAACCTCGCCG
CCCGCGCCCTCGCAGCTGGCGGATCACGGGCGGTTGGAATCTTGCTGACGACCCGTCTGACTCACGGCATGTCCACAACC
TTCACCGCACTGGTAGGCACACTGCGGGAGCATAGTCGCCCATTCGTCCTCGACACCGCGATCGAGAATGACGAGGAATC
CCTACGCGCCGCTCTGGCTCGACTCCGCGGCTACAGCGTCCAGGCAACCATCTTGCTGGCACAACGAGCCAAATCCGTTG
CCATCGCATCCCAGCAACGCCACGGCGACAAGCTGGTCTCTGTCATCGCAGGGCACCCCGACTCCCCCGAGTCACCAATT
GTCGCGATCGACCAAGCACTGGGAGCCCGTCTAGCCACTAAACACCTCATCGAACAGGGCTACCGGAATCTTCTCCATGT
CCCTGGCGACCTGGAATGGCAGGACGCGTCAGAACGGCTTTCCTCCTACATCGCCGTCTGCAAAGAGGTCGGCATCGACC
CTGCATGGATGCCAGCCAATTCCTGGGGAGCCGAAGCCGGGGCAGCCGTGGCTGAAGAACTTATCGCCGGACATCTCCCC
GACGCGGTTCTCGCTGGCAACGATGACATCGCCATTGGCATGTGTCACACATTCCTCACTCATGGCGTGAGGATCCCCGA
CGACATCGGCATTGTGGGATTCGACGACATCCCTCAAGCTCGCTGGATGACCCCATCCCTCACCACGGTCGTCCAGGACT
TCGAAGCACTCGGACGCCAAGCAGTCGAGGCCGCGGAAGCCCTCATCGCTGGAGAGGCTCCGGAGTCGTGCCAGCTCTCC
CCCACTTTGGCGGTGAGGCACTCGTCACGCCGGCACTAA

Upstream 100 bases:

>100_bases
GTGAACCATTCAACCGTGCTTTTTGCAATAATGAGCATCATCGTCGATCGACCCGATCGACGTCGCTCAGCAGCCCCGCC
CAACCTCCAGGTGAATCGCC

Downstream 100 bases:

>100_bases
AGGACGTTCCAATATGAAGTTATATCCTGCTATGTATAATGATGCATTTTGCGGTGTCACTCCGACACCGAGCCCTCCCT
CAATGCCAATATCATCAAGT

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MSSRATLSDVAKACGVSSQTVSRALNDSPLVRPETRAKVMAAVETVGYEPNLAARALAAGGSRAVGILLTTRLTHGMSTT
FTALVGTLREHSRPFVLDTAIENDEESLRAALARLRGYSVQATILLAQRAKSVAIASQQRHGDKLVSVIAGHPDSPESPI
VAIDQALGARLATKHLIEQGYRNLLHVPGDLEWQDASERLSSYIAVCKEVGIDPAWMPANSWGAEAGAAVAEELIAGHLP
DAVLAGNDDIAIGMCHTFLTHGVRIPDDIGIVGFDDIPQARWMTPSLTTVVQDFEALGRQAVEAAEALIAGEAPESCQLS
PTLAVRHSSRRH

Sequences:

>Translated_332_residues
MSSRATLSDVAKACGVSSQTVSRALNDSPLVRPETRAKVMAAVETVGYEPNLAARALAAGGSRAVGILLTTRLTHGMSTT
FTALVGTLREHSRPFVLDTAIENDEESLRAALARLRGYSVQATILLAQRAKSVAIASQQRHGDKLVSVIAGHPDSPESPI
VAIDQALGARLATKHLIEQGYRNLLHVPGDLEWQDASERLSSYIAVCKEVGIDPAWMPANSWGAEAGAAVAEELIAGHLP
DAVLAGNDDIAIGMCHTFLTHGVRIPDDIGIVGFDDIPQARWMTPSLTTVVQDFEALGRQAVEAAEALIAGEAPESCQLS
PTLAVRHSSRRH
>Mature_331_residues
SSRATLSDVAKACGVSSQTVSRALNDSPLVRPETRAKVMAAVETVGYEPNLAARALAAGGSRAVGILLTTRLTHGMSTTF
TALVGTLREHSRPFVLDTAIENDEESLRAALARLRGYSVQATILLAQRAKSVAIASQQRHGDKLVSVIAGHPDSPESPIV
AIDQALGARLATKHLIEQGYRNLLHVPGDLEWQDASERLSSYIAVCKEVGIDPAWMPANSWGAEAGAAVAEELIAGHLPD
AVLAGNDDIAIGMCHTFLTHGVRIPDDIGIVGFDDIPQARWMTPSLTTVVQDFEALGRQAVEAAEALIAGEAPESCQLSP
TLAVRHSSRRH

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787948, Length=332, Percent_Identity=28.6144578313253, Blast_Score=134, Evalue=9e-33,
Organism=Escherichia coli, GI1790194, Length=330, Percent_Identity=28.7878787878788, Blast_Score=128, Evalue=6e-31,
Organism=Escherichia coli, GI1788474, Length=337, Percent_Identity=30.2670623145401, Blast_Score=127, Evalue=9e-31,
Organism=Escherichia coli, GI1789202, Length=335, Percent_Identity=31.9402985074627, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1786540, Length=335, Percent_Identity=29.2537313432836, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI1790369, Length=302, Percent_Identity=28.8079470198675, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789068, Length=308, Percent_Identity=28.8961038961039, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1787580, Length=334, Percent_Identity=25.1497005988024, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI48994940, Length=341, Percent_Identity=26.3929618768328, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1790715, Length=322, Percent_Identity=23.2919254658385, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35209; Mature: 35078

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRATLSDVAKACGVSSQTVSRALNDSPLVRPETRAKVMAAVETVGYEPNLAARALAAG
CCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCC
GSRAVGILLTTRLTHGMSTTFTALVGTLREHSRPFVLDTAIENDEESLRAALARLRGYSV
CCCEEEHEEEHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHCCCCH
QATILLAQRAKSVAIASQQRHGDKLVSVIAGHPDSPESPIVAIDQALGARLATKHLIEQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
YRNLLHVPGDLEWQDASERLSSYIAVCKEVGIDPAWMPANSWGAEAGAAVAEELIAGHLP
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
DAVLAGNDDIAIGMCHTFLTHGVRIPDDIGIVGFDDIPQARWMTPSLTTVVQDFEALGRQ
CCEEECCCCEEHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVEAAEALIAGEAPESCQLSPTLAVRHSSRRH
HHHHHHHHHCCCCCCCCCCCCHHEECCCCCCC
>Mature Secondary Structure 
SSRATLSDVAKACGVSSQTVSRALNDSPLVRPETRAKVMAAVETVGYEPNLAARALAAG
CCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCC
GSRAVGILLTTRLTHGMSTTFTALVGTLREHSRPFVLDTAIENDEESLRAALARLRGYSV
CCCEEEHEEEHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHCCCHHHHHHHHHHHCCCCH
QATILLAQRAKSVAIASQQRHGDKLVSVIAGHPDSPESPIVAIDQALGARLATKHLIEQG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
YRNLLHVPGDLEWQDASERLSSYIAVCKEVGIDPAWMPANSWGAEAGAAVAEELIAGHLP
HHHHEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
DAVLAGNDDIAIGMCHTFLTHGVRIPDDIGIVGFDDIPQARWMTPSLTTVVQDFEALGRQ
CCEEECCCCEEHHHHHHHHHCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHHHHHHHHHH
AVEAAEALIAGEAPESCQLSPTLAVRHSSRRH
HHHHHHHHHCCCCCCCCCCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA