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The map label for this gene is argF

Identifier: 50842070

GI number: 50842070

Start: 645968

End: 646972

Strand: Direct

Name: argF

Synonym: PPA0584

Alternate gene names: 50842070

Gene position: 645968-646972 (Clockwise)

Preceding gene: 50842069

Following gene: 50842071

Centisome position: 25.23

GC content: 60.2

Gene sequence:

>1005_bases
ATGGCTTTTAATCTGAAGAATCGTCATTTGTTGTCGTTGGCGCATCATAGTACTGAGGAAATCAAGTTCCTTGTCGGCTT
GGCTGCAGATCTCAAAGAAGCCAAGTACTGCGGGACGGAGCAGCCGCTCCTCAAGGGGAAGAACATTGCCCTCATTTTCG
AGAAGGCGTCGACTCGTACCCGTTGTGCCTTCGAGGTGGCGGCTCACGACCAGGGTGCTCACGTGACCTACTTGGGGCCC
AGCGGATCTCACATTGGCCACAAGGAGTCCATGAAGGACACGGCCCGCGTCCTGGGGCGTATGTATGACGGCATCGAGTA
CCGCGGGTTCCACCAGTCGGTGGTGGAAGAGCTGGCTGAATACGCAGGTGTCCCGGTTTTCAATGGCCTGACCGACGAGT
TCCACCCGACCCAGATGCTCGCTGACGCTCTCACCATGCGTGAGCATGCTGAGAAACCGTTCCACGAGATCAAGTTCGCT
TACGTCGGCGACGGGCGCAACAACATGGGCCGTTCGCTCCTGCTGCTGGGTGCCAAGCTCGGCATGGATGTGCGTATCGG
TGCTCCCGAGGGGCTGCAGCCTGAGGCCGAATTGGTGGAACGTTGCCGCGAGTGGGCCGAGCAGTCCGGTGGCAAGGTGC
TGGTGACCGCTTCTGCGGAGGAGGCCGTGCGCGATGTCGACTTCATCCACACCGACGTGTGGGTGTCCATGGGCGAGCCT
GTGGAGAGCTGGAGCGAGCGGGTCGGCACCTTGCTGCCGTTCCAGATCAATGATGCGTTGGTCAAGGCTGCTGGTAACCC
GCGCGTCAAGGTGATGCACTGCCTGCCGGCATTCCACAACAGTGAGACGGCGGTTGGTGCTGAGGTTGCCAAGACCTACC
CGGAGCTGGCCAATGGCATTGAGGTGACGGAGTCCGTGTTCGAGTCGCCTGCCTGCATCGCGTTTGACCAAGCGGAAAAT
CGCATGCACACCATCAAGGCCGTCCTGGTGTCCTCCCTGGCCTGA

Upstream 100 bases:

>100_bases
ATCACTCGTGATCCGGTGGATTACTGATAAGTCCCAAAGCGAAACAGGTAATATGCGTCCGCCGCACGAAGCGGACATAT
CAAGGAAGGAATATCAGATC

Downstream 100 bases:

>100_bases
CGAGTGATCTGAGGAAGAATATGAGGATTTTGGTTGCACTTGGTGGTAACGCGTTGCTGCGGCGTGGCGAGACCATGACG
GCGGAGAATCAGCGTGCCAA

Product: ornithine carbamoyltransferase, catabolic

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 334; Mature: 333

Protein sequence:

>334_residues
MAFNLKNRHLLSLAHHSTEEIKFLVGLAADLKEAKYCGTEQPLLKGKNIALIFEKASTRTRCAFEVAAHDQGAHVTYLGP
SGSHIGHKESMKDTARVLGRMYDGIEYRGFHQSVVEELAEYAGVPVFNGLTDEFHPTQMLADALTMREHAEKPFHEIKFA
YVGDGRNNMGRSLLLLGAKLGMDVRIGAPEGLQPEAELVERCREWAEQSGGKVLVTASAEEAVRDVDFIHTDVWVSMGEP
VESWSERVGTLLPFQINDALVKAAGNPRVKVMHCLPAFHNSETAVGAEVAKTYPELANGIEVTESVFESPACIAFDQAEN
RMHTIKAVLVSSLA

Sequences:

>Translated_334_residues
MAFNLKNRHLLSLAHHSTEEIKFLVGLAADLKEAKYCGTEQPLLKGKNIALIFEKASTRTRCAFEVAAHDQGAHVTYLGP
SGSHIGHKESMKDTARVLGRMYDGIEYRGFHQSVVEELAEYAGVPVFNGLTDEFHPTQMLADALTMREHAEKPFHEIKFA
YVGDGRNNMGRSLLLLGAKLGMDVRIGAPEGLQPEAELVERCREWAEQSGGKVLVTASAEEAVRDVDFIHTDVWVSMGEP
VESWSERVGTLLPFQINDALVKAAGNPRVKVMHCLPAFHNSETAVGAEVAKTYPELANGIEVTESVFESPACIAFDQAEN
RMHTIKAVLVSSLA
>Mature_333_residues
AFNLKNRHLLSLAHHSTEEIKFLVGLAADLKEAKYCGTEQPLLKGKNIALIFEKASTRTRCAFEVAAHDQGAHVTYLGPS
GSHIGHKESMKDTARVLGRMYDGIEYRGFHQSVVEELAEYAGVPVFNGLTDEFHPTQMLADALTMREHAEKPFHEIKFAY
VGDGRNNMGRSLLLLGAKLGMDVRIGAPEGLQPEAELVERCREWAEQSGGKVLVTASAEEAVRDVDFIHTDVWVSMGEPV
ESWSERVGTLLPFQINDALVKAAGNPRVKVMHCLPAFHNSETAVGAEVAKTYPELANGIEVTESVFESPACIAFDQAENR
MHTIKAVLVSSLA

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=331, Percent_Identity=37.4622356495468, Blast_Score=210, Evalue=1e-54,
Organism=Homo sapiens, GI18105007, Length=342, Percent_Identity=24.5614035087719, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1790703, Length=328, Percent_Identity=58.8414634146341, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786469, Length=331, Percent_Identity=57.7039274924471, Blast_Score=390, Evalue=1e-110,
Organism=Escherichia coli, GI2367364, Length=315, Percent_Identity=29.5238095238095, Blast_Score=78, Evalue=8e-16,
Organism=Escherichia coli, GI48994908, Length=369, Percent_Identity=25.2032520325203, Blast_Score=76, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6322373, Length=330, Percent_Identity=31.2121212121212, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6322331, Length=207, Percent_Identity=29.4685990338164, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24642586, Length=340, Percent_Identity=25.8823529411765, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2066 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): OTC_PROAC (Q6AA77)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_055297.1
- ProteinModelPortal:   Q6AA77
- SMR:   Q6AA77
- GeneID:   2931731
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0584
- NMPDR:   fig|267747.1.peg.574
- HOGENOM:   HBG579429
- OMA:   MTPYQIN
- ProtClustDB:   CLSK2763386
- BioCyc:   PACN267747:PPA0584-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 36653; Mature: 36522

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 108-108 BINDING 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFNLKNRHLLSLAHHSTEEIKFLVGLAADLKEAKYCGTEQPLLKGKNIALIFEKASTRT
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCC
RCAFEVAAHDQGAHVTYLGPSGSHIGHKESMKDTARVLGRMYDGIEYRGFHQSVVEELAE
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YAGVPVFNGLTDEFHPTQMLADALTMREHAEKPFHEIKFAYVGDGRNNMGRSLLLLGAKL
HCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCEEEEEHHHC
GMDVRIGAPEGLQPEAELVERCREWAEQSGGKVLVTASAEEAVRDVDFIHTDVWVSMGEP
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHEECCCC
VESWSERVGTLLPFQINDALVKAAGNPRVKVMHCLPAFHNSETAVGAEVAKTYPELANGI
HHHHHHHHCCCCCEEECHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCH
EVTESVFESPACIAFDQAENRMHTIKAVLVSSLA
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AFNLKNRHLLSLAHHSTEEIKFLVGLAADLKEAKYCGTEQPLLKGKNIALIFEKASTRT
CCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCEEEEEECCCCCC
RCAFEVAAHDQGAHVTYLGPSGSHIGHKESMKDTARVLGRMYDGIEYRGFHQSVVEELAE
EEEEEEEECCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
YAGVPVFNGLTDEFHPTQMLADALTMREHAEKPFHEIKFAYVGDGRNNMGRSLLLLGAKL
HCCCCEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHEEEEEEECCCCCCCCEEEEEHHHC
GMDVRIGAPEGLQPEAELVERCREWAEQSGGKVLVTASAEEAVRDVDFIHTDVWVSMGEP
CCEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHEECCCC
VESWSERVGTLLPFQINDALVKAAGNPRVKVMHCLPAFHNSETAVGAEVAKTYPELANGI
HHHHHHHHCCCCCEEECHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCH
EVTESVFESPACIAFDQAENRMHTIKAVLVSSLA
HHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA