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The map label for this gene is prs [H]

Identifier: 50842015

GI number: 50842015

Start: 584797

End: 585765

Strand: Direct

Name: prs [H]

Synonym: PPA0531

Alternate gene names: 50842015

Gene position: 584797-585765 (Clockwise)

Preceding gene: 50842014

Following gene: 50842016

Centisome position: 22.84

GC content: 57.89

Gene sequence:

>969_bases
GTGAGTGGAACCACCCGTCCGAACAACAAGCACCTCATGATTTGCTCAGGACGGGCCTATCCCGAACTTGCTGAGCAGAT
CGCTGCCGAACTCGGCGTCGAGCTTGTCCCGTCGCGTCAAGTTACGTACGCGAACTCGGAGATCTACGTGCAGTTCCAGG
AGTCTGTGCGCGGCTGCGACGCTTTCGTTATCCAATCCCACAGTGCGCCGGTAAACGAGGCCATGATGGAGCAGCTCATC
ATGATCGACGCGCTCAAGAGGGCTTCGGCTAAGCGCATCACCGTGGTTTCTCCGTTCTATCCCTACGCTCGTCAGGACAA
GAAGCACCTGGGTCGCGAGCCCATTTCCGCCCGCCTTATGGCCGATCTTTATCAGGCTGCTGGAGCCGACCGCATCATGT
CAGTCGATCTGCACGCCGCCCAGATCCAAGGATTCTTCGACGGCCCTGTCGACCACTTGTGGGCCCTTCCGGTGCTCGCC
GACTACGTCACCTCCAAATATGACAATTCTGACATGGTTGTCGTCTCCCCGGACGCCGGCCGGGTGCGCCTGGCTGACAT
GTGGACCGACAAGATTGGCTGCCAGCTGGCGATCATTCATAAGCGTCGTGATCCGAACAAGGCCAACGAGACAACCACCC
ATGAGGTTGTCGGTGAGGTTGAGGGTAAGACTTGTTTGCTTGTCGACGACATGGTCGACACTGCGGGCACGATGTGCCAG
GCTGCCCTAGCTCTTAAAAAGCATGGCGCCAAGCGGGTTATTGCCGCTGCCACTCACCCGATCCTGTCGGACCCGGCCGC
TGAACGGATCAATAACTCGCCCATCGAAGAACTCATCGTCACCAACACTCTCAAGATTCGCGACGATATTGACATTCCTG
CCATGACGCTTCTGTCCATCGCCCCGCTTATTGCTAAGGCGATCCAGGAGGTTTTCGAGGACGGGTCTGTGACGAGTCTG
TTCCGCTGA

Upstream 100 bases:

>100_bases
TTGCCGGATCCGTCCTTTCCCGGGAGTGCGCCGCTCCCGCCACCGTCTCGGACATCACGTCCGACAGCCAAGACGACACC
CTGAACCCGGAGGCTGACCA

Downstream 100 bases:

>100_bases
CGTTGTTTGGGGGTACAGCGTTGTGCCCCTTCGTGCCGGTCCGAATGGCCCTCTCGGTTGATACCGTCTATCTGTCAAGG
TCAGATCTTGGCCGGGAGCG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSGTTRPNNKHLMICSGRAYPELAEQIAAELGVELVPSRQVTYANSEIYVQFQESVRGCDAFVIQSHSAPVNEAMMEQLI
MIDALKRASAKRITVVSPFYPYARQDKKHLGREPISARLMADLYQAAGADRIMSVDLHAAQIQGFFDGPVDHLWALPVLA
DYVTSKYDNSDMVVVSPDAGRVRLADMWTDKIGCQLAIIHKRRDPNKANETTTHEVVGEVEGKTCLLVDDMVDTAGTMCQ
AALALKKHGAKRVIAAATHPILSDPAAERINNSPIEELIVTNTLKIRDDIDIPAMTLLSIAPLIAKAIQEVFEDGSVTSL
FR

Sequences:

>Translated_322_residues
MSGTTRPNNKHLMICSGRAYPELAEQIAAELGVELVPSRQVTYANSEIYVQFQESVRGCDAFVIQSHSAPVNEAMMEQLI
MIDALKRASAKRITVVSPFYPYARQDKKHLGREPISARLMADLYQAAGADRIMSVDLHAAQIQGFFDGPVDHLWALPVLA
DYVTSKYDNSDMVVVSPDAGRVRLADMWTDKIGCQLAIIHKRRDPNKANETTTHEVVGEVEGKTCLLVDDMVDTAGTMCQ
AALALKKHGAKRVIAAATHPILSDPAAERINNSPIEELIVTNTLKIRDDIDIPAMTLLSIAPLIAKAIQEVFEDGSVTSL
FR
>Mature_321_residues
SGTTRPNNKHLMICSGRAYPELAEQIAAELGVELVPSRQVTYANSEIYVQFQESVRGCDAFVIQSHSAPVNEAMMEQLIM
IDALKRASAKRITVVSPFYPYARQDKKHLGREPISARLMADLYQAAGADRIMSVDLHAAQIQGFFDGPVDHLWALPVLAD
YVTSKYDNSDMVVVSPDAGRVRLADMWTDKIGCQLAIIHKRRDPNKANETTTHEVVGEVEGKTCLLVDDMVDTAGTMCQA
ALALKKHGAKRVIAAATHPILSDPAAERINNSPIEELIVTNTLKIRDDIDIPAMTLLSIAPLIAKAIQEVFEDGSVTSLF
R

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=319, Percent_Identity=43.8871473354232, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI4506129, Length=313, Percent_Identity=42.1725239616613, Blast_Score=249, Evalue=3e-66,
Organism=Homo sapiens, GI84875539, Length=316, Percent_Identity=42.0886075949367, Blast_Score=243, Evalue=1e-64,
Organism=Homo sapiens, GI28557709, Length=317, Percent_Identity=42.5867507886435, Blast_Score=241, Evalue=9e-64,
Organism=Homo sapiens, GI194018537, Length=339, Percent_Identity=35.1032448377581, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4506133, Length=332, Percent_Identity=33.433734939759, Blast_Score=159, Evalue=2e-39,
Organism=Homo sapiens, GI310128524, Length=145, Percent_Identity=30.3448275862069, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310115209, Length=145, Percent_Identity=30.3448275862069, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310118259, Length=145, Percent_Identity=30.3448275862069, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI310119946, Length=145, Percent_Identity=30.3448275862069, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787458, Length=317, Percent_Identity=44.1640378548896, Blast_Score=263, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI25149168, Length=313, Percent_Identity=41.85303514377, Blast_Score=244, Evalue=5e-65,
Organism=Caenorhabditis elegans, GI17554704, Length=308, Percent_Identity=42.5324675324675, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI17554702, Length=317, Percent_Identity=42.2712933753943, Blast_Score=243, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI71989924, Length=313, Percent_Identity=41.85303514377, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17570245, Length=342, Percent_Identity=31.8713450292398, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319403, Length=313, Percent_Identity=43.7699680511182, Blast_Score=258, Evalue=1e-69,
Organism=Saccharomyces cerevisiae, GI6320946, Length=312, Percent_Identity=43.2692307692308, Blast_Score=255, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6321776, Length=302, Percent_Identity=42.3841059602649, Blast_Score=242, Evalue=5e-65,
Organism=Saccharomyces cerevisiae, GI6322667, Length=195, Percent_Identity=40.5128205128205, Blast_Score=147, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6324511, Length=89, Percent_Identity=42.6966292134831, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21355239, Length=315, Percent_Identity=42.8571428571429, Blast_Score=250, Evalue=9e-67,
Organism=Drosophila melanogaster, GI45551540, Length=338, Percent_Identity=39.9408284023669, Blast_Score=238, Evalue=5e-63,
Organism=Drosophila melanogaster, GI281362873, Length=354, Percent_Identity=30.5084745762712, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24651454, Length=354, Percent_Identity=30.5084745762712, Blast_Score=169, Evalue=2e-42,
Organism=Drosophila melanogaster, GI24651458, Length=354, Percent_Identity=30.5084745762712, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651456, Length=354, Percent_Identity=30.5084745762712, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24651462, Length=200, Percent_Identity=34, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24651464, Length=200, Percent_Identity=34, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45552010, Length=200, Percent_Identity=34, Blast_Score=122, Evalue=3e-28,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 35353; Mature: 35222

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTTRPNNKHLMICSGRAYPELAEQIAAELGVELVPSRQVTYANSEIYVQFQESVRGCD
CCCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCEEEEECCEEEEEEHHHHCCCC
AFVIQSHSAPVNEAMMEQLIMIDALKRASAKRITVVSPFYPYARQDKKHLGREPISARLM
EEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHH
ADLYQAAGADRIMSVDLHAAQIQGFFDGPVDHLWALPVLADYVTSKYDNSDMVVVSPDAG
HHHHHHCCCCCEEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
RVRLADMWTDKIGCQLAIIHKRRDPNKANETTTHEVVGEVEGKTCLLVDDMVDTAGTMCQ
CEEEEEHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
AALALKKHGAKRVIAAATHPILSDPAAERINNSPIEELIVTNTLKIRDDIDIPAMTLLSI
HHHHHHHCCCHHEEHHHCCCCCCCHHHHHCCCCCHHHHHHHCEEEECCCCCCHHHHHHHH
APLIAKAIQEVFEDGSVTSLFR
HHHHHHHHHHHHCCCCCHHHCC
>Mature Secondary Structure 
SGTTRPNNKHLMICSGRAYPELAEQIAAELGVELVPSRQVTYANSEIYVQFQESVRGCD
CCCCCCCCCEEEEECCCCCHHHHHHHHHHCCCEECCCCEEEEECCEEEEEEHHHHCCCC
AFVIQSHSAPVNEAMMEQLIMIDALKRASAKRITVVSPFYPYARQDKKHLGREPISARLM
EEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHCCCCCHHHHHH
ADLYQAAGADRIMSVDLHAAQIQGFFDGPVDHLWALPVLADYVTSKYDNSDMVVVSPDAG
HHHHHHCCCCCEEEEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
RVRLADMWTDKIGCQLAIIHKRRDPNKANETTTHEVVGEVEGKTCLLVDDMVDTAGTMCQ
CEEEEEHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHH
AALALKKHGAKRVIAAATHPILSDPAAERINNSPIEELIVTNTLKIRDDIDIPAMTLLSI
HHHHHHHCCCHHEEHHHCCCCCCCHHHHHCCCCCHHHHHHHCEEEECCCCCCHHHHHHHH
APLIAKAIQEVFEDGSVTSLFR
HHHHHHHHHHHHCCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11572948; 12692562 [H]