Click here to switch to the map view.

The map label for this gene is ulaC [H]

Identifier: 50841930

GI number: 50841930

Start: 492858

End: 493787

Strand: Reverse

Name: ulaC [H]

Synonym: PPA0447

Alternate gene names: 50841930

Gene position: 493787-492858 (Counterclockwise)

Preceding gene: 161621798

Following gene: 50841929

Centisome position: 19.29

GC content: 58.92

Gene sequence:

>930_bases
GTGCGGGTGGGGAGCCAACCATTCCCTCAGGGCCGCCCAAGAAGCCGCCCACACAATGCTGAACCACCGCGACCACTGGA
AACAGGTAATGGCCTGAAAACCGTGGCCTTCTACCCTCACGAGGGCGTACGCTATGGCTCCCACAGCGATGGAAAGGCCC
CCACGGTGATTGACCTGACAGACCTCCTGCCTCCAGCCGCCATTCGTCTGGACGCACACGTCAACGATTGGCGAGAAGCT
CTCCAGGCCGTCGGGCATCTGCTGGTGGCCACCGACACCGCTACCCCCAGATACACGCAGGCCATGATCGATAATCTCGA
GAAAAATGGTCCTTACATCGTCGTCGCCCCTGGATTTGCACTGGCCCATGCCAGACCGGACTCCTCAGTGCTGCGGACAG
GCATGTCGTGGATACGTCTTGACGAACCGGTGGCATTCGGCCATGAGACGAACGATCCTGTAACTCTAGTAGCAGGACTC
GCAGCCACAGACGCCTCTGCCCACCAAAATGTTCTGGCCGCGTTGGCGAGCGCACTGGCCGATCCAAACAGACGCAACGC
CCTCGACACTGCGACGACTCCCCAGGAGGTCGTGTCGATCCTCTCCAACGAGGCCGGACACCGTTCAGCTGAGGCATCGA
CATCCCAGAATCTCTTGCTCACGGTCTGCGGCAACGGACTAGGCACCAGTCTGTTTCTCAAAAACACCACTGAGCAAGTC
CTTGATGCTTGGGCGTGGACCCCTTACCTGTCAGTTGAGGCAACCGACACCATCTCTGCAAGAGGGCGCTGCAGCGAAGC
AGACGCCATTTTGACGTCGGGAGCCATTGCTCAAACTCTCGGTGAGCTATCCATTCCCGTCGAAGTCATCGACGATTTCA
CATCAGTGTCGCAGGTCGATGCTGCGTTACGCCGCATTTACGACGTCTAA

Upstream 100 bases:

>100_bases
ATCTGAAACGTATTCAAGTATCAGGGCACTAGGGACTCGTCGTGGCCACCCTGCGCGACATCCTCAAGCTCAACACCACC
CCCGCCGCTAACGAAGTACA

Downstream 100 bases:

>100_bases
TCGCTGACCATCAGTCGCCACGCCTACATCATCCCCGGAGACTAACCTCATGAACATCGCCCTGGCCATCGTCCAATTCC
TCGTAAACGAGATCCTCAGC

Product: phosphotransferase system protein, mannitol/fructose-specific IIA subunit

Products: Protein Histidine; Sugar Phosphate. [C]

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 309; Mature: 309

Protein sequence:

>309_residues
MRVGSQPFPQGRPRSRPHNAEPPRPLETGNGLKTVAFYPHEGVRYGSHSDGKAPTVIDLTDLLPPAAIRLDAHVNDWREA
LQAVGHLLVATDTATPRYTQAMIDNLEKNGPYIVVAPGFALAHARPDSSVLRTGMSWIRLDEPVAFGHETNDPVTLVAGL
AATDASAHQNVLAALASALADPNRRNALDTATTPQEVVSILSNEAGHRSAEASTSQNLLLTVCGNGLGTSLFLKNTTEQV
LDAWAWTPYLSVEATDTISARGRCSEADAILTSGAIAQTLGELSIPVEVIDDFTSVSQVDAALRRIYDV

Sequences:

>Translated_309_residues
MRVGSQPFPQGRPRSRPHNAEPPRPLETGNGLKTVAFYPHEGVRYGSHSDGKAPTVIDLTDLLPPAAIRLDAHVNDWREA
LQAVGHLLVATDTATPRYTQAMIDNLEKNGPYIVVAPGFALAHARPDSSVLRTGMSWIRLDEPVAFGHETNDPVTLVAGL
AATDASAHQNVLAALASALADPNRRNALDTATTPQEVVSILSNEAGHRSAEASTSQNLLLTVCGNGLGTSLFLKNTTEQV
LDAWAWTPYLSVEATDTISARGRCSEADAILTSGAIAQTLGELSIPVEVIDDFTSVSQVDAALRRIYDV
>Mature_309_residues
MRVGSQPFPQGRPRSRPHNAEPPRPLETGNGLKTVAFYPHEGVRYGSHSDGKAPTVIDLTDLLPPAAIRLDAHVNDWREA
LQAVGHLLVATDTATPRYTQAMIDNLEKNGPYIVVAPGFALAHARPDSSVLRTGMSWIRLDEPVAFGHETNDPVTLVAGL
AATDASAHQNVLAALASALADPNRRNALDTATTPQEVVSILSNEAGHRSAEASTSQNLLLTVCGNGLGTSLFLKNTTEQV
LDAWAWTPYLSVEATDTISARGRCSEADAILTSGAIAQTLGELSIPVEVIDDFTSVSQVDAALRRIYDV

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=137, Percent_Identity=40.1459854014599, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1790755, Length=127, Percent_Identity=37.7952755905512, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1789302, Length=144, Percent_Identity=34.0277777777778, Blast_Score=86, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69 [C]

Molecular weight: Translated: 32940; Mature: 32940

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVGSQPFPQGRPRSRPHNAEPPRPLETGNGLKTVAFYPHEGVRYGSHSDGKAPTVIDLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEHH
DLLPPAAIRLDAHVNDWREALQAVGHLLVATDTATPRYTQAMIDNLEKNGPYIVVAPGFA
HCCCCCEEEEECCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCEE
LAHARPDSSVLRTGMSWIRLDEPVAFGHETNDPVTLVAGLAATDASAHQNVLAALASALA
EECCCCCHHHHHHCCHHEEECCCEECCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHC
DPNRRNALDTATTPQEVVSILSNEAGHRSAEASTSQNLLLTVCGNGLGTSLFLKNTTEQV
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCHHHH
LDAWAWTPYLSVEATDTISARGRCSEADAILTSGAIAQTLGELSIPVEVIDDFTSVSQVD
HHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHH
AALRRIYDV
HHHHHHHCC
>Mature Secondary Structure
MRVGSQPFPQGRPRSRPHNAEPPRPLETGNGLKTVAFYPHEGVRYGSHSDGKAPTVIDLT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEHH
DLLPPAAIRLDAHVNDWREALQAVGHLLVATDTATPRYTQAMIDNLEKNGPYIVVAPGFA
HCCCCCEEEEECCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHCCCCEEEEECCEE
LAHARPDSSVLRTGMSWIRLDEPVAFGHETNDPVTLVAGLAATDASAHQNVLAALASALA
EECCCCCHHHHHHCCHHEEECCCEECCCCCCCCEEEEEEHHHCCCHHHHHHHHHHHHHHC
DPNRRNALDTATTPQEVVSILSNEAGHRSAEASTSQNLLLTVCGNGLGTSLFLKNTTEQV
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCCEEEECCCHHHH
LDAWAWTPYLSVEATDTISARGRCSEADAILTSGAIAQTLGELSIPVEVIDDFTSVSQVD
HHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHH
AALRRIYDV
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protein N-Phosphohistidine; Sugar [C]

Specific reaction: Protein N-Phosphohistidine + Sugar = Protein Histidine + Sugar Phosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]