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The map label for this gene is folK [H]

Identifier: 50841762

GI number: 50841762

Start: 322857

End: 323414

Strand: Direct

Name: folK [H]

Synonym: PPA0273

Alternate gene names: 50841762

Gene position: 322857-323414 (Clockwise)

Preceding gene: 50841761

Following gene: 50841763

Centisome position: 12.61

GC content: 60.93

Gene sequence:

>558_bases
GTGAAAACCGTATTCAGCCTGGGTTCCAATATGGGAGACCCTTTTGCCCATCTGCGAGACGCCGTCCGCCTGATCGCCGA
AACTCCCGGTATTCACGACGTCGTCGTTTCGCCCGTCTATGTCACCGCTCCGATCGGGGGAGTGGAGCAGGATGACTTCC
TCAACCTCATCGTCGTTGCGACTAGCGACCTTCCTCCGTCAGTCCTGTTGGAGCGCGCCCATGTCATCGAGGCAGCCCAC
GGGCGCGATCACACCATCCCGAATGGTCCTCGCACCCTCGACGTTGATCTCATTCGCGTTGGCGACGAGACCGTCACCAC
CGACGAGTTGACCCTTCCACACCCCCGTGCACATAAGCGCGCCTTCGTTCTGGTGCCGTGGCGCGACGCCGACCCTGAGG
CTGAATTGCCCGGGTACGGTCGGATCGCCGATCTGGTCGCCTCCTTCGATGCTGACGAGCTGGCTGACGCCGTTAAGCCT
TATTCGCGTCAGATTGACGTAGCTGGGGTCACCGTTGCAGGAGAATGGGACGAGAGGCACGACGAGGAGGATGTATGA

Upstream 100 bases:

>100_bases
GTCCGCGTGTCCATTCTGTCCAGGTAACCGTTCACAAGCCACATGCCCCCGTAGGGGTTGCCCTCGACGACGTAGCCGTC
ACGTACCGAAAGGATGCCGA

Downstream 100 bases:

>100_bases
CACGGAGCTTGCCACCCGAGTGGGATGACGACCGGCGACCTGGCGGCGGTAACCCTCAAGGCAACATCGCTCCGACCGAT
TGGAAGGTCATCATCGTGGC

Product: putative hydroxymethyldihydropteridine pyrophosphokinase

Products: NA

Alternate protein names: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase; PPPK; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase; HPPK [H]

Number of amino acids: Translated: 185; Mature: 185

Protein sequence:

>185_residues
MKTVFSLGSNMGDPFAHLRDAVRLIAETPGIHDVVVSPVYVTAPIGGVEQDDFLNLIVVATSDLPPSVLLERAHVIEAAH
GRDHTIPNGPRTLDVDLIRVGDETVTTDELTLPHPRAHKRAFVLVPWRDADPEAELPGYGRIADLVASFDADELADAVKP
YSRQIDVAGVTVAGEWDERHDEEDV

Sequences:

>Translated_185_residues
MKTVFSLGSNMGDPFAHLRDAVRLIAETPGIHDVVVSPVYVTAPIGGVEQDDFLNLIVVATSDLPPSVLLERAHVIEAAH
GRDHTIPNGPRTLDVDLIRVGDETVTTDELTLPHPRAHKRAFVLVPWRDADPEAELPGYGRIADLVASFDADELADAVKP
YSRQIDVAGVTVAGEWDERHDEEDV
>Mature_185_residues
MKTVFSLGSNMGDPFAHLRDAVRLIAETPGIHDVVVSPVYVTAPIGGVEQDDFLNLIVVATSDLPPSVLLERAHVIEAAH
GRDHTIPNGPRTLDVDLIRVGDETVTTDELTLPHPRAHKRAFVLVPWRDADPEAELPGYGRIADLVASFDADELADAVKP
YSRQIDVAGVTVAGEWDERHDEEDV

Specific function: Dihydrofolate biosynthesis; first step. [C]

COG id: COG0801

COG function: function code H; 7,8-dihydro-6-hydroxymethylpterin-pyrophosphokinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HPPK family [H]

Homologues:

Organism=Escherichia coli, GI1786335, Length=133, Percent_Identity=35.3383458646617, Blast_Score=88, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6324073, Length=131, Percent_Identity=31.2977099236641, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000550 [H]

Pfam domain/function: PF01288 HPPK [H]

EC number: =2.7.6.3 [H]

Molecular weight: Translated: 20154; Mature: 20154

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00082 EXTRADIOL_DIOXYGENAS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTVFSLGSNMGDPFAHLRDAVRLIAETPGIHDVVVSPVYVTAPIGGVEQDDFLNLIVVA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEECCEEEECCCCCCCCCCCEEEEEEE
TSDLPPSVLLERAHVIEAAHGRDHTIPNGPRTLDVDLIRVGDETVTTDELTLPHPRAHKR
ECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCCCCCE
AFVLVPWRDADPEAELPGYGRIADLVASFDADELADAVKPYSRQIDVAGVTVAGEWDERH
EEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCHHHCEEEEEEEEEECCCCCCC
DEEDV
CCCCC
>Mature Secondary Structure
MKTVFSLGSNMGDPFAHLRDAVRLIAETPGIHDVVVSPVYVTAPIGGVEQDDFLNLIVVA
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHEEECCEEEECCCCCCCCCCCEEEEEEE
TSDLPPSVLLERAHVIEAAHGRDHTIPNGPRTLDVDLIRVGDETVTTDELTLPHPRAHKR
ECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECCCEECCCCEECCCCCCCCE
AFVLVPWRDADPEAELPGYGRIADLVASFDADELADAVKPYSRQIDVAGVTVAGEWDERH
EEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHHHHCHHHCEEEEEEEEEECCCCCCC
DEEDV
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11234002 [H]