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The map label for this gene is nth [H]

Identifier: 50841716

GI number: 50841716

Start: 277186

End: 278112

Strand: Direct

Name: nth [H]

Synonym: PPA0223

Alternate gene names: 50841716

Gene position: 277186-278112 (Clockwise)

Preceding gene: 50841714

Following gene: 50841717

Centisome position: 10.83

GC content: 62.46

Gene sequence:

>927_bases
ATGGCCATGATTCGGGCGGCCGAGGCTGCGCCGAGAGTTGCGAAGAACGGTTGGGTCGCCGCTGTTGCCACCACATTTGC
CATCCGAACATCTCCCCTCATGAGCACGTGCATCGTTCGACCATTCGTGGCCGGGCGTGTGCCTACATTGTGGCAGCTGT
CACATTCGGTGCTGACACCGGGCCTCGATTCTCCTCCTATGCTTAGCTCCATGGCAGCATCCCCTAGCGCATCTCGACCT
CAACGGGAGGACTGCCGCGCGTGCGCCAACGAGGTGCGAGCACTCCTTGCCAAGGCCTATCCAGACGCTCACTGCGAGTT
GAATTACGCCGGTCCGTACCAATTGCTCGTTGCCACGGTGCTGTCGGCCCAAACGACGGATCGGCGAGTCAATACCGTCA
CACCCACGTTGTTCAACCGTTGGCCTGGCCCCCAGGCCCTGGCCGATGCTGACATTGGTGAAGTCGAGACAGTAGTCGCC
CCGCTGGGATGTGGACCAACCCGAGCAGCGCGCCTGGTGTCGATGGGCGCGAAGCTCGTCGACAATTTTGACGGTGCCAT
CCCTGACGATCTTGACTCTCTTGTGACCCTGCCCGGAGTCGGTCGTAAGACCGCCAATGTTGTTTTAGGTAATGCCTTCG
GCATCCCCGGAATCACCCCGGACACCCACGTCATGCGGGTATCTCGACGTCTGGGCTGGACCGATGCGACTACCCCCGCC
AAGGTGGAAACCGACCTGGCTGAGCTTTTTGACCCGTCTGAATGGGTGATGTTGTGTCACCGCCTCATCTGGCACGGGCG
GCGGCGCTGTCACTCGCGGCGTCCTGCCTGCGGGGTATGCCCGGTTGCCGAGTGGTGCCCGTCCTTCGGGGAAGGCCCAA
CGGATCCCGAGGAGGCCGCCACGTTAGTCCGGGAGCCGCGTCGATGA

Upstream 100 bases:

>100_bases
AGTGAGCTTCACCTTTCCTTCGACGACGAGGTACAGGTTGTCAGCTGAGTCTCCGGCCTCGAAGATCACGGTATTTCGCG
GGCAGACTGACGCCTCACAC

Downstream 100 bases:

>100_bases
GGGGGATGAAGGTTTTCAGCGCGGTGATGGCCGCCTTGATGTTTGCTGGCTGCGGGGGAGATGCGGGCATGGCTCATCAG
CGCGAGGATGCCGGAATACC

Product: putative endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MAMIRAAEAAPRVAKNGWVAAVATTFAIRTSPLMSTCIVRPFVAGRVPTLWQLSHSVLTPGLDSPPMLSSMAASPSASRP
QREDCRACANEVRALLAKAYPDAHCELNYAGPYQLLVATVLSAQTTDRRVNTVTPTLFNRWPGPQALADADIGEVETVVA
PLGCGPTRAARLVSMGAKLVDNFDGAIPDDLDSLVTLPGVGRKTANVVLGNAFGIPGITPDTHVMRVSRRLGWTDATTPA
KVETDLAELFDPSEWVMLCHRLIWHGRRRCHSRRPACGVCPVAEWCPSFGEGPTDPEEAATLVREPRR

Sequences:

>Translated_308_residues
MAMIRAAEAAPRVAKNGWVAAVATTFAIRTSPLMSTCIVRPFVAGRVPTLWQLSHSVLTPGLDSPPMLSSMAASPSASRP
QREDCRACANEVRALLAKAYPDAHCELNYAGPYQLLVATVLSAQTTDRRVNTVTPTLFNRWPGPQALADADIGEVETVVA
PLGCGPTRAARLVSMGAKLVDNFDGAIPDDLDSLVTLPGVGRKTANVVLGNAFGIPGITPDTHVMRVSRRLGWTDATTPA
KVETDLAELFDPSEWVMLCHRLIWHGRRRCHSRRPACGVCPVAEWCPSFGEGPTDPEEAATLVREPRR
>Mature_307_residues
AMIRAAEAAPRVAKNGWVAAVATTFAIRTSPLMSTCIVRPFVAGRVPTLWQLSHSVLTPGLDSPPMLSSMAASPSASRPQ
REDCRACANEVRALLAKAYPDAHCELNYAGPYQLLVATVLSAQTTDRRVNTVTPTLFNRWPGPQALADADIGEVETVVAP
LGCGPTRAARLVSMGAKLVDNFDGAIPDDLDSLVTLPGVGRKTANVVLGNAFGIPGITPDTHVMRVSRRLGWTDATTPAK
VETDLAELFDPSEWVMLCHRLIWHGRRRCHSRRPACGVCPVAEWCPSFGEGPTDPEEAATLVREPRR

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=192, Percent_Identity=31.25, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787920, Length=197, Percent_Identity=37.5634517766497, Blast_Score=139, Evalue=3e-34,
Organism=Caenorhabditis elegans, GI17554540, Length=177, Percent_Identity=38.9830508474576, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324530, Length=202, Percent_Identity=29.7029702970297, Blast_Score=74, Evalue=2e-14,
Organism=Drosophila melanogaster, GI45550361, Length=179, Percent_Identity=32.9608938547486, Blast_Score=94, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 33152; Mature: 33021

Theoretical pI: Translated: 7.91; Mature: 7.91

Prosite motif: PS00589 PTS_HPR_SER ; PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAMIRAAEAAPRVAKNGWVAAVATTFAIRTSPLMSTCIVRPFVAGRVPTLWQLSHSVLTP
CCCCCHHHHCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GLDSPPMLSSMAASPSASRPQREDCRACANEVRALLAKAYPDAHCELNYAGPYQLLVATV
CCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
LSAQTTDRRVNTVTPTLFNRWPGPQALADADIGEVETVVAPLGCGPTRAARLVSMGAKLV
HHHHCCCCHHHCCCHHHHHCCCCCHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
DNFDGAIPDDLDSLVTLPGVGRKTANVVLGNAFGIPGITPDTHVMRVSRRLGWTDATTPA
HHCCCCCCCCHHHHHCCCCCCCCHHHEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
KVETDLAELFDPSEWVMLCHRLIWHGRRRCHSRRPACGVCPVAEWCPSFGEGPTDPEEAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHH
TLVREPRR
HHHHCCCC
>Mature Secondary Structure 
AMIRAAEAAPRVAKNGWVAAVATTFAIRTSPLMSTCIVRPFVAGRVPTLWQLSHSVLTP
CCCCHHHHCCHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCC
GLDSPPMLSSMAASPSASRPQREDCRACANEVRALLAKAYPDAHCELNYAGPYQLLVATV
CCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHH
LSAQTTDRRVNTVTPTLFNRWPGPQALADADIGEVETVVAPLGCGPTRAARLVSMGAKLV
HHHHCCCCHHHCCCHHHHHCCCCCHHHHCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHH
DNFDGAIPDDLDSLVTLPGVGRKTANVVLGNAFGIPGITPDTHVMRVSRRLGWTDATTPA
HHCCCCCCCCHHHHHCCCCCCCCHHHEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
KVETDLAELFDPSEWVMLCHRLIWHGRRRCHSRRPACGVCPVAEWCPSFGEGPTDPEEAA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCHHHHH
TLVREPRR
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]