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The map label for this gene is lysS

Identifier: 50841670

GI number: 50841670

Start: 220963

End: 222495

Strand: Direct

Name: lysS

Synonym: PPA0181

Alternate gene names: 50841670

Gene position: 220963-222495 (Clockwise)

Preceding gene: 50841669

Following gene: 50841672

Centisome position: 8.63

GC content: 61.58

Gene sequence:

>1533_bases
GTGAGTTCTGACACTGCATCCAGCCAGTCCCCTGACGTCTCCGAGCAGATGCAGGTCCGCCTCGACAAGCGGGCCCGGAT
CATCGCTGACGGAGGCCAGGCTTATCCGGTCGATTTGCTACGCGACCACACCCTGGTCCAGGTTCGTCAGAAATACACCG
ACGACACCGGGGAGCTGACCTTGGCGCCTGGCGAGGAGACCACCGATGAGGTCACCGTCGGCGGACGTGTCGTCTTTGTC
CGCAATACCGGCAAACTGTGTTTCGCCAGCTTGCAGGACGGGTTTACCGAGACTTCCAACGGTGAGCGGTTGCAGATCAT
GCTTTCCAAAGCCGAGGTGGGCGATGAGTCCTTGGCTGCGTGGAAGTCCGACGTTGACCTGGGTGACCACGTCTGGGTGC
GTGGCCGAGTCGTCGTCTCTCGTCGTGGTGAGTTATCCATCATGGCTGCTGAGTGGCGGATGGCCTCCAAGGCTCTGCGT
CCGCTGCCGACCCTCCATAAGGAGCTCTCGGAGGAGAGCCGGGTGCGTCGTCGCTACGCGGATCTCGTCGTTCGTGAAGA
GTCTCGCCAGATGGTGCGCACCCGCGCCGTTATCACCCGCGCGGTGCGTCGCGCCCTCGACGAGCGCGGTTTCCTTGAGA
TCGAGACTCCGATCCTGCAGTTGGTCCACGGTGGTGCCGCAGCCCGCCCGTTTAATACCCACCTCAATGCCTTCGACATT
GACATGAGTTTGCGTATCGCTCTTGAGCTCTATCTCAAGCGGACGATGGTCGGCGGCACCGATCGGGTCTACGAGATGGG
TCGGGTCTTCCGCAACGAGGGTATTGATTCCAGCCACTCGGCCGAGTTCTCTATGCTCGAGGCCTACATGAGCTGGGGCA
ACCAATTCACTATTGCTGAGGTGATCAAGGACATCATCATGGCGGTGGCCGACGCCCTCGACGTCCATCAGATTGAGACC
CCCAAGGGCACTATCGACCTCGACGGGCAGTGGCGTTGGGTCAGCGTCTACCCAGGCCTGTCGGAAAAACTAGGGCGTGA
AGTCACCCTTGACACCCCGTTGGAGGAGTTGCAGTCCATCGCCGCTGAGCACGAGATTGAGATCGACCAGTCTTGGGAGG
CCGGCAAGGTCGTTATGGAGCTTTTCGGTGAACTCGTCGAGCCGGGCCTTATCAACCCCACCTTCGTCTGTGATTACCCG
GCTATCGCCCAGCCGCTGGCCCGTCCGCACCGCGACGATCCTCGCATGGTTGAGGCCTGGGACCTCATCATTGGCGGGAT
GGAGCGCGGTACTGGCTTCTCCGAGCTCGTCGACCCAGTCATCCAGCGCGAAGTGCTTACCAAGCAATCCCTCGCTGCTG
CCGCGGGTGACCCTGAGGCGATGCAGCTTGACGAGGACTTCCTCGAGGCCCTGGAATTCGGCTGCCCGCCCATGGGTGGT
CTGGGGCTGGGTGTCGACCGGCTGGTGATGCTCTTTACCAACGCTGGGATCCGCGAGACGATCCTCTTCCCGCTGCTCAA
GCCGGAACACTGA

Upstream 100 bases:

>100_bases
GATAAGGAATTAGGTAGCTAGTTAGAGGTGATGACAACTTCGAAGGCGGGATAGCATAGGTGAAATCGTCGTCAGCTTGT
TGCTTCGATAAGATTCCCTG

Downstream 100 bases:

>100_bases
CTGAAGGCCCGCCGGGACATCCCTGGTGGGCCTCCCGCTCACTTCCACAGGGTAGCGACGCCGAATCCAGCAGCCATGAG
CCCCATACCGATGAGGATGT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 510; Mature: 509

Protein sequence:

>510_residues
MSSDTASSQSPDVSEQMQVRLDKRARIIADGGQAYPVDLLRDHTLVQVRQKYTDDTGELTLAPGEETTDEVTVGGRVVFV
RNTGKLCFASLQDGFTETSNGERLQIMLSKAEVGDESLAAWKSDVDLGDHVWVRGRVVVSRRGELSIMAAEWRMASKALR
PLPTLHKELSEESRVRRRYADLVVREESRQMVRTRAVITRAVRRALDERGFLEIETPILQLVHGGAAARPFNTHLNAFDI
DMSLRIALELYLKRTMVGGTDRVYEMGRVFRNEGIDSSHSAEFSMLEAYMSWGNQFTIAEVIKDIIMAVADALDVHQIET
PKGTIDLDGQWRWVSVYPGLSEKLGREVTLDTPLEELQSIAAEHEIEIDQSWEAGKVVMELFGELVEPGLINPTFVCDYP
AIAQPLARPHRDDPRMVEAWDLIIGGMERGTGFSELVDPVIQREVLTKQSLAAAAGDPEAMQLDEDFLEALEFGCPPMGG
LGLGVDRLVMLFTNAGIRETILFPLLKPEH

Sequences:

>Translated_510_residues
MSSDTASSQSPDVSEQMQVRLDKRARIIADGGQAYPVDLLRDHTLVQVRQKYTDDTGELTLAPGEETTDEVTVGGRVVFV
RNTGKLCFASLQDGFTETSNGERLQIMLSKAEVGDESLAAWKSDVDLGDHVWVRGRVVVSRRGELSIMAAEWRMASKALR
PLPTLHKELSEESRVRRRYADLVVREESRQMVRTRAVITRAVRRALDERGFLEIETPILQLVHGGAAARPFNTHLNAFDI
DMSLRIALELYLKRTMVGGTDRVYEMGRVFRNEGIDSSHSAEFSMLEAYMSWGNQFTIAEVIKDIIMAVADALDVHQIET
PKGTIDLDGQWRWVSVYPGLSEKLGREVTLDTPLEELQSIAAEHEIEIDQSWEAGKVVMELFGELVEPGLINPTFVCDYP
AIAQPLARPHRDDPRMVEAWDLIIGGMERGTGFSELVDPVIQREVLTKQSLAAAAGDPEAMQLDEDFLEALEFGCPPMGG
LGLGVDRLVMLFTNAGIRETILFPLLKPEH
>Mature_509_residues
SSDTASSQSPDVSEQMQVRLDKRARIIADGGQAYPVDLLRDHTLVQVRQKYTDDTGELTLAPGEETTDEVTVGGRVVFVR
NTGKLCFASLQDGFTETSNGERLQIMLSKAEVGDESLAAWKSDVDLGDHVWVRGRVVVSRRGELSIMAAEWRMASKALRP
LPTLHKELSEESRVRRRYADLVVREESRQMVRTRAVITRAVRRALDERGFLEIETPILQLVHGGAAARPFNTHLNAFDID
MSLRIALELYLKRTMVGGTDRVYEMGRVFRNEGIDSSHSAEFSMLEAYMSWGNQFTIAEVIKDIIMAVADALDVHQIETP
KGTIDLDGQWRWVSVYPGLSEKLGREVTLDTPLEELQSIAAEHEIEIDQSWEAGKVVMELFGELVEPGLINPTFVCDYPA
IAQPLARPHRDDPRMVEAWDLIIGGMERGTGFSELVDPVIQREVLTKQSLAAAAGDPEAMQLDEDFLEALEFGCPPMGGL
GLGVDRLVMLFTNAGIRETILFPLLKPEH

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=520, Percent_Identity=35, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI194272210, Length=520, Percent_Identity=35, Blast_Score=305, Evalue=5e-83,
Organism=Homo sapiens, GI45439306, Length=492, Percent_Identity=23.5772357723577, Blast_Score=84, Evalue=3e-16,
Organism=Escherichia coli, GI1790571, Length=515, Percent_Identity=39.2233009708738, Blast_Score=349, Evalue=3e-97,
Organism=Escherichia coli, GI1789256, Length=505, Percent_Identity=39.2079207920792, Blast_Score=347, Evalue=1e-96,
Organism=Escherichia coli, GI87082379, Length=319, Percent_Identity=28.8401253918495, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788173, Length=261, Percent_Identity=27.5862068965517, Blast_Score=79, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=524, Percent_Identity=34.7328244274809, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17535927, Length=526, Percent_Identity=34.6007604562738, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71994340, Length=513, Percent_Identity=35.0877192982456, Blast_Score=285, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI32566633, Length=187, Percent_Identity=28.8770053475936, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320242, Length=484, Percent_Identity=34.9173553719008, Blast_Score=265, Evalue=2e-71,
Organism=Saccharomyces cerevisiae, GI6324256, Length=529, Percent_Identity=27.9773156899811, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24640849, Length=471, Percent_Identity=37.791932059448, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24640851, Length=471, Percent_Identity=37.791932059448, Blast_Score=317, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56873; Mature: 56742

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDTASSQSPDVSEQMQVRLDKRARIIADGGQAYPVDLLRDHTLVQVRQKYTDDTGELT
CCCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHCHHHHHHHHHHCCCCCCEE
LAPGEETTDEVTVGGRVVFVRNTGKLCFASLQDGFTETSNGERLQIMLSKAEVGDESLAA
ECCCCCCCCCEEECCEEEEEECCCCEEEHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHH
WKSDVDLGDHVWVRGRVVVSRRGELSIMAAEWRMASKALRPLPTLHKELSEESRVRRRYA
HHCCCCCCCCEEEEEEEEEECCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DLVVREESRQMVRTRAVITRAVRRALDERGFLEIETPILQLVHGGAAARPFNTHLNAFDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCCCCCCCCCEEEC
DMSLRIALELYLKRTMVGGTDRVYEMGRVFRNEGIDSSHSAEFSMLEAYMSWGNQFTIAE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEHHH
VIKDIIMAVADALDVHQIETPKGTIDLDGQWRWVSVYPGLSEKLGREVTLDTPLEELQSI
HHHHHHHHHHHHHHHHEECCCCCEEEECCCEEEEEECCCHHHHHCCEEEECCCHHHHHHH
AAEHEIEIDQSWEAGKVVMELFGELVEPGLINPTFVCDYPAIAQPLARPHRDDPRMVEAW
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHCCCCCCCCHHHHH
DLIIGGMERGTGFSELVDPVIQREVLTKQSLAAAAGDPEAMQLDEDFLEALEFGCPPMGG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCCC
LGLGVDRLVMLFTNAGIRETILFPLLKPEH
CCCCHHHHHHHHHCCCHHHHHHHCCCCCCC
>Mature Secondary Structure 
SSDTASSQSPDVSEQMQVRLDKRARIIADGGQAYPVDLLRDHTLVQVRQKYTDDTGELT
CCCCCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCHHHHHCHHHHHHHHHHCCCCCCEE
LAPGEETTDEVTVGGRVVFVRNTGKLCFASLQDGFTETSNGERLQIMLSKAEVGDESLAA
ECCCCCCCCCEEECCEEEEEECCCCEEEHHHHCCCCCCCCCCEEEEEEECCCCCCHHHHH
WKSDVDLGDHVWVRGRVVVSRRGELSIMAAEWRMASKALRPLPTLHKELSEESRVRRRYA
HHCCCCCCCCEEEEEEEEEECCCCEEEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
DLVVREESRQMVRTRAVITRAVRRALDERGFLEIETPILQLVHGGAAARPFNTHLNAFDI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECHHHHHHHHCCCCCCCCCCCCCEEEC
DMSLRIALELYLKRTMVGGTDRVYEMGRVFRNEGIDSSHSAEFSMLEAYMSWGNQFTIAE
CHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEHHH
VIKDIIMAVADALDVHQIETPKGTIDLDGQWRWVSVYPGLSEKLGREVTLDTPLEELQSI
HHHHHHHHHHHHHHHHEECCCCCEEEECCCEEEEEECCCHHHHHCCEEEECCCHHHHHHH
AAEHEIEIDQSWEAGKVVMELFGELVEPGLINPTFVCDYPAIAQPLARPHRDDPRMVEAW
HHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEECCHHHHHHHHCCCCCCCCHHHHH
DLIIGGMERGTGFSELVDPVIQREVLTKQSLAAAAGDPEAMQLDEDFLEALEFGCPPMGG
HHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHCCCCCCC
LGLGVDRLVMLFTNAGIRETILFPLLKPEH
CCCCHHHHHHHHHCCCHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA