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The map label for this gene is lacA [C]

Identifier: 50841623

GI number: 50841623

Start: 160926

End: 161543

Strand: Direct

Name: lacA [C]

Synonym: PPA0134

Alternate gene names: 50841623

Gene position: 160926-161543 (Clockwise)

Preceding gene: 50841621

Following gene: 50841624

Centisome position: 6.29

GC content: 62.78

Gene sequence:

>618_bases
ATGGGTGAACCACGGATCATCGACACCGCCGACTTGGACGACGGTGTGACCATCGGCGACGGTTCGTCGATCTGGCATCT
TTCCCAAGTTCGCAGCGAGGCCGTCCTCGGGCAGAACGTCGTCGTGGGCCGCGGTGCCTACATCGGCGAGGGCGTCCACG
TTGGCGACAACTGCAAGATCCAGAATTACGCGTTGGTTTACGAGCCCGCGAAGCTGGAAGACGGGGTATTCATCGGACCG
GCAGTGGTGCTTACCAACGACCACTTCCCGCGGGCTATCAACCCCGACGGCTCCCTCAAATCTGCTGACGACTGGGAGCA
GGTGGGTGTGACCTGCAAGCGTGGCTGCTCTGTTGGCGCCCGTTCGGTGTGCATTGCGCCTGTCACTATTGGTGAGTGGG
CCACGGTGGCTGCCGGCTCGGTCGTCACCAAGGATGTCCCGGCATATGCCTTGGTGGCCGGCGTCCCGGCCCGTCGCATT
AAGTGGGTGGGCCGTTCCGGATTCCCGCTAGATCCCGACGGTGAGAACAAGTGGATCGACTCGCGTACCGGAGACCGGTT
CATCGAGGATCCTGCCACCGATACGCTGACCCTGGTTAGCGAGGGCGAGCAGGCCTAA

Upstream 100 bases:

>100_bases
GCCACGCCGAATTTTCGGCGTCGCCGTGCAGGGGCGACGGGCCTGTCGGGCTAGCACGAGTAAGCTGGCCTTGATGTAAC
CGGCATGAAAGGCGTCCCAC

Downstream 100 bases:

>100_bases
GGCCGTCGTCCCCCAATACCCCCTCGTGGGTGCTACGGCGCCCACAAACCGGACCTTCCGTCCGAATAGTTAGGAGCTTT
CACGTGACCGCCCAGTTCAT

Product: putative acetyltransferase

Products: CoA; aacetyl-beta-D-galactoside [C]

Alternate protein names: Hexapaptide Repeat-Containing Transferase; Transferase Hexapeptide Repeat Containing Protein; Hexapeptide Transferase Family Protein; Acetyl/Acyl Transferase Related Protein; WxcM-Like Protein; WxcM Domain-Containing Protein; WbbJ Protein; WblC Protein; Hexapeptide Repeat-Containing Transferase; Acetyltransferases; Acetyl / Acyl Transferase Related Protein; Acyltransferase; Bacterial Transferase Hexapeptide Repeat Protein; WxcM-Like; Acetyltransferase Protein; Isoleucine Patch Superfamily Acetyltransferase; Transferase Hexapeptide Repeat; CysE Serine O-Acetyltransferase; Glucose-Fructose Oxidoreductase; Oxidoreductase Domain Protein; Acetyltransferase WbpD; UDP-3-O-(3-Hydroxymyristoyl)-Like Protein; Transferase Hexapeptide Repeat-Containing Protein; Carbonic Anhydrase/Acetyltransferase; Hexapeptide Transferase Family Protein Acetyltransferase; NDP-Sugar Acetyltransferase; Serine Acetyltransferase-Like Protein; Acetyl Transferase/Isomerase; Bifunctional Acetyl Transferase/Isomerase; N-Acetylglucosamine-1-Phosphate Uridyltransferase; Transferase Protein; Hexapeptide Transferase; Carbohydrate Kinase Rmoresistant Glucokinase; Lipopolysaccharide Biosynthesis Protein; Bacterial Transferase Hexapeptide-Like Protein; Transferase Hexapeptide Family; Acetyltransferase-Like Protein; UDP-3-O-[3-Hydroxymyristoyl] Glucosamine N-Acyltransferase; Acetyl/Acyl Transferase-Like Protein; Carbonic Anhydrases/Acetyltransferases

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MGEPRIIDTADLDDGVTIGDGSSIWHLSQVRSEAVLGQNVVVGRGAYIGEGVHVGDNCKIQNYALVYEPAKLEDGVFIGP
AVVLTNDHFPRAINPDGSLKSADDWEQVGVTCKRGCSVGARSVCIAPVTIGEWATVAAGSVVTKDVPAYALVAGVPARRI
KWVGRSGFPLDPDGENKWIDSRTGDRFIEDPATDTLTLVSEGEQA

Sequences:

>Translated_205_residues
MGEPRIIDTADLDDGVTIGDGSSIWHLSQVRSEAVLGQNVVVGRGAYIGEGVHVGDNCKIQNYALVYEPAKLEDGVFIGP
AVVLTNDHFPRAINPDGSLKSADDWEQVGVTCKRGCSVGARSVCIAPVTIGEWATVAAGSVVTKDVPAYALVAGVPARRI
KWVGRSGFPLDPDGENKWIDSRTGDRFIEDPATDTLTLVSEGEQA
>Mature_204_residues
GEPRIIDTADLDDGVTIGDGSSIWHLSQVRSEAVLGQNVVVGRGAYIGEGVHVGDNCKIQNYALVYEPAKLEDGVFIGPA
VVLTNDHFPRAINPDGSLKSADDWEQVGVTCKRGCSVGARSVCIAPVTIGEWATVAAGSVVTKDVPAYALVAGVPARRIK
WVGRSGFPLDPDGENKWIDSRTGDRFIEDPATDTLTLVSEGEQA

Specific function: May Assist Cellular Detoxification By Acetylating Nonmetabolizable Pyranosides, Thereby Preventing Their Reentry Into The Cell. [C]

COG id: COG0110

COG function: function code R; Acetyltransferase (isoleucine patch superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.3.1.18 [C]

Molecular weight: Translated: 21717; Mature: 21586

Theoretical pI: Translated: 4.41; Mature: 4.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEPRIIDTADLDDGVTIGDGSSIWHLSQVRSEAVLGQNVVVGRGAYIGEGVHVGDNCKI
CCCCCEEECCCCCCCEEECCCCCEEHHHHHHHHHHCCCCEEEECCCEECCCEEECCCCEE
QNYALVYEPAKLEDGVFIGPAVVLTNDHFPRAINPDGSLKSADDWEQVGVTCKRGCSVGA
EEEEEEECCCCCCCCEEECCEEEEECCCCCCEECCCCCCCCCCCHHHHCCEEECCCCCCC
RSVCIAPVTIGEWATVAAGSVVTKDVPAYALVAGVPARRIKWVGRSGFPLDPDGENKWID
CEEEEEEEECCCCHHHHCCCEEECCCCCEEEEECCCHHHEEEECCCCCCCCCCCCCCEEC
SRTGDRFIEDPATDTLTLVSEGEQA
CCCCCCEECCCCCCEEEEEECCCCC
>Mature Secondary Structure 
GEPRIIDTADLDDGVTIGDGSSIWHLSQVRSEAVLGQNVVVGRGAYIGEGVHVGDNCKI
CCCCEEECCCCCCCEEECCCCCEEHHHHHHHHHHCCCCEEEECCCEECCCEEECCCCEE
QNYALVYEPAKLEDGVFIGPAVVLTNDHFPRAINPDGSLKSADDWEQVGVTCKRGCSVGA
EEEEEEECCCCCCCCEEECCEEEEECCCCCCEECCCCCCCCCCCHHHHCCEEECCCCCCC
RSVCIAPVTIGEWATVAAGSVVTKDVPAYALVAGVPARRIKWVGRSGFPLDPDGENKWID
CEEEEEEEECCCCHHHHCCCEEECCCCCEEEEECCCHHHEEEECCCCCCCCCCCCCCEEC
SRTGDRFIEDPATDTLTLVSEGEQA
CCCCCCEECCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: MgSO4; Mn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 1500 {isopropyl} 1200 {isopropyl} 770 {isopropyl} 360 {isopropyl} 0.18 {acetyl-CoA}} 0.049 {acetyl-CoA}} 0.045 {acetyl-CoA}} 0.04 {acetyl-CoA}} [C]

Substrates: Acetyl-CoA; a beta-D-galactoside [C]

Specific reaction: Acetyl-CoA + a beta-D-galactoside = CoA + aacetyl-beta-D-galactoside [C]

General reaction: Acyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; Acetyl-CoA; Co2+; CoA; Cu2+; MgSO4; Mn2+; p-Chloromercuribenzoate; Zn2+ [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA