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The map label for this gene is thiE

Identifier: 50841603

GI number: 50841603

Start: 135027

End: 135680

Strand: Direct

Name: thiE

Synonym: PPA0112

Alternate gene names: 50841603

Gene position: 135027-135680 (Clockwise)

Preceding gene: 50841602

Following gene: 50841605

Centisome position: 5.27

GC content: 65.9

Gene sequence:

>654_bases
ATGACTTTAGACCTGCGGTGCTACTTGGTGACGTCGGGGATTGATCGGCACACCATCGAGACTGCGGCAGCGGCCGCTGG
GGCGGGTGCCGGGATGGTGCAGGTACGGGCCAAGGAATTGTCCACTCGTGATCTGCTCAGCCTGGTTTTGCAGGTCGGCG
AGGCGGTTCGTCGAGCGAACCCCGCGACGCGGGTGGTCGTGGATGACCGCGCCGATGTTGCCTGGGCGGCCATACGGGCG
CGGGGGAACGTCCACGGGGTGCACGTCGGATTGACGGATTTGCCGGTGCGTGATGCGCGGGCGATGCTGGGCCCCGACGC
GATCGTCGGTTACACCACGGGGACCTTGGACCTCGTGAGATCGGCCGAACCGTTCGCTGATGCGCTCGACTATGTGGGTG
CCGGACCCTTCCGACCCACCCCGACCAAGGAATCGGGCCGCTCCCCGCTCGGCGTTCAGGGGTATCCGGCCTTAGTAGGG
GCGAGCAGTCTGCCGGTTGTCGCTATCGGTGACGTCCAGGTGGCCGATGTCCCTGTTTTGGCGGCCACCGGTGTGGCCGG
TGTCGCGATGGTGCGGGCCATCATGGCCTCCGATGACCCTGCTGCAGTGGTGCGGCAGGTTGTGCAGTCTTTCGATGAGG
TTCGGGTTAGCTGA

Upstream 100 bases:

>100_bases
GGGAGGTTGCAGCAGCGCGTGCTGGCGGACCCGGAAGTTTTCGGGTGCAGCTGCGTGACGCCCTTGACAAGGCGGCGAGA
ACCGCAAAATAGGAGATGAG

Downstream 100 bases:

>100_bases
TGCTCTGAGGAGGTTTGGTGGGCTGACGTTGTTTCCCCCGTTGGCGTAGGCCATTGAGGTTGCGGTCCCGTCTTCCTCCG
CGGGGCTGTGACTTTATCCC

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 217; Mature: 216

Protein sequence:

>217_residues
MTLDLRCYLVTSGIDRHTIETAAAAAGAGAGMVQVRAKELSTRDLLSLVLQVGEAVRRANPATRVVVDDRADVAWAAIRA
RGNVHGVHVGLTDLPVRDARAMLGPDAIVGYTTGTLDLVRSAEPFADALDYVGAGPFRPTPTKESGRSPLGVQGYPALVG
ASSLPVVAIGDVQVADVPVLAATGVAGVAMVRAIMASDDPAAVVRQVVQSFDEVRVS

Sequences:

>Translated_217_residues
MTLDLRCYLVTSGIDRHTIETAAAAAGAGAGMVQVRAKELSTRDLLSLVLQVGEAVRRANPATRVVVDDRADVAWAAIRA
RGNVHGVHVGLTDLPVRDARAMLGPDAIVGYTTGTLDLVRSAEPFADALDYVGAGPFRPTPTKESGRSPLGVQGYPALVG
ASSLPVVAIGDVQVADVPVLAATGVAGVAMVRAIMASDDPAAVVRQVVQSFDEVRVS
>Mature_216_residues
TLDLRCYLVTSGIDRHTIETAAAAAGAGAGMVQVRAKELSTRDLLSLVLQVGEAVRRANPATRVVVDDRADVAWAAIRAR
GNVHGVHVGLTDLPVRDARAMLGPDAIVGYTTGTLDLVRSAEPFADALDYVGAGPFRPTPTKESGRSPLGVQGYPALVGA
SSLPVVAIGDVQVADVPVLAATGVAGVAMVRAIMASDDPAAVVRQVVQSFDEVRVS

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=203, Percent_Identity=30.0492610837438, Blast_Score=88, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 22418; Mature: 22287

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLDLRCYLVTSGIDRHTIETAAAAAGAGAGMVQVRAKELSTRDLLSLVLQVGEAVRRAN
CEEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCC
PATRVVVDDRADVAWAAIRARGNVHGVHVGLTDLPVRDARAMLGPDAIVGYTTGTLDLVR
CCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEECCCHHHHHH
SAEPFADALDYVGAGPFRPTPTKESGRSPLGVQGYPALVGASSLPVVAIGDVQVADVPVL
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEECCEEECCCCEE
AATGVAGVAMVRAIMASDDPAAVVRQVVQSFDEVRVS
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLDLRCYLVTSGIDRHTIETAAAAAGAGAGMVQVRAKELSTRDLLSLVLQVGEAVRRAN
EEEEEEEEEECCCCHHHHHHHHHHCCCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHCC
PATRVVVDDRADVAWAAIRARGNVHGVHVGLTDLPVRDARAMLGPDAIVGYTTGTLDLVR
CCEEEEEECCCCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHCCCCCEEEECCCHHHHHH
SAEPFADALDYVGAGPFRPTPTKESGRSPLGVQGYPALVGASSLPVVAIGDVQVADVPVL
CCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCEEEECCEEECCCCEE
AATGVAGVAMVRAIMASDDPAAVVRQVVQSFDEVRVS
HHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA