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The map label for this gene is purR [H]

Identifier: 50841579

GI number: 50841579

Start: 99230

End: 100351

Strand: Direct

Name: purR [H]

Synonym: PPA0088

Alternate gene names: 50841579

Gene position: 99230-100351 (Clockwise)

Preceding gene: 50841578

Following gene: 50841580

Centisome position: 3.88

GC content: 65.95

Gene sequence:

>1122_bases
TTGCCCTGGGCGTCGTCATCGCCGTGCTCGGTGTCTACAACCGCCGATACGTGTGACATGTCCGCGAGCGGCTTCTCCCC
GCCTCTGACCGGTCGGGCACCGACAATGGCCGACGTGGCCAAGAGGGCTGCCGTGTCGCCCCAAACGGTGTCGCGGGTGG
TTCGAGGCCAAACGAACGTATCCGAGGTAACCCGACTCAAGGTGGAATTGGCGATCGACGAATTGGGCTACCGGCGCACT
GGCATCGCTCGAGCGCTGGTGACCGGGCACACGATGACGCTAGGGGTCCTGACCCAGGGCTCGGTCGCTTACTCCCGTGC
TGCCTACGTGGACGCTATGACGTCGGCCGCCCGCCGCCACGGATATTACGTGGCCGCAGCGTCGGTGACGTCGACTGCCC
CCGAAGCGGTGTGTCAGAGCATCGAACACCTGCGTCAGCAGGGAGTCGACGGTATCGTCGTGGCGGTCCCGGTTTATGAC
GTCGATGCTCTCGCGCAAGCGGCAAGGGGGCTACCTGCTGTCGTATTTGGTGGCTCGTCGAGCGAGGCGGTGCCGGATGG
CTTCTCGGACGGCACTGGGCACCACGCGGTGTGCATCGACCAGGAGCGGATAGGCGTACTGGCCACGGAGCACCTGCTAG
GACTTGGGCACGACACCGTATGGCATGTTGCCGGACCCAGCACTTGGGCCGACGCGTCGGGCCGTCAGGCGGGCTGGGCG
GCGACGCTGGCCAAACACGGGCGTCCCGAGCCGCCGGTGCTCATCGGTGACTGGAGTGCCGAGTCCGGCTGGAGGCTCGG
GACCCTGCTGGCTCGGATGGAGGAGGTAACGGCTATCTTCGCGGCGAGCGACGACATGGCTTTCGGGATTCTGCGCGCTC
TGGTTGACCGAGGCCGACGTGTACCCGAAGATGTGTCGGTGGTCGGCGCCGACGATGTGCCGCTGGCTGCCTTCGCTCCG
GTTGCACTGACCACGGTGCGCCAGTCCTTCCAGGATCTCGGAACCGCCGCTATCGATCTGCTTGTCGACATGGTCGAACG
ACCCGACCGAGAGCACCACGGCGTGCTGATGGAGCCGGAACTGGTGCTGAGATCATCGACTGCGCCGCGTCATGCTGGCT
GA

Upstream 100 bases:

>100_bases
GTGTTCGGGTGGCTGGCGTTTGCGCTGGTACTGGGCGCATGCGTCGTCGTCATGGTGGCGATGCGCACTACGGGATGGGT
CCAGGCAGGAATCATGGCTC

Downstream 100 bases:

>100_bases
GTCATGGCGTCTGCACTACCGCTCACCTGCAGCGAAGTGGGAGGCTCACGGGCTGCCGATCGGCAACGGCCGTCTCGGGG
CCGTCCTACGGGGGGAAATC

Product: putative transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 373; Mature: 372

Protein sequence:

>373_residues
MPWASSSPCSVSTTADTCDMSASGFSPPLTGRAPTMADVAKRAAVSPQTVSRVVRGQTNVSEVTRLKVELAIDELGYRRT
GIARALVTGHTMTLGVLTQGSVAYSRAAYVDAMTSAARRHGYYVAAASVTSTAPEAVCQSIEHLRQQGVDGIVVAVPVYD
VDALAQAARGLPAVVFGGSSSEAVPDGFSDGTGHHAVCIDQERIGVLATEHLLGLGHDTVWHVAGPSTWADASGRQAGWA
ATLAKHGRPEPPVLIGDWSAESGWRLGTLLARMEEVTAIFAASDDMAFGILRALVDRGRRVPEDVSVVGADDVPLAAFAP
VALTTVRQSFQDLGTAAIDLLVDMVERPDREHHGVLMEPELVLRSSTAPRHAG

Sequences:

>Translated_373_residues
MPWASSSPCSVSTTADTCDMSASGFSPPLTGRAPTMADVAKRAAVSPQTVSRVVRGQTNVSEVTRLKVELAIDELGYRRT
GIARALVTGHTMTLGVLTQGSVAYSRAAYVDAMTSAARRHGYYVAAASVTSTAPEAVCQSIEHLRQQGVDGIVVAVPVYD
VDALAQAARGLPAVVFGGSSSEAVPDGFSDGTGHHAVCIDQERIGVLATEHLLGLGHDTVWHVAGPSTWADASGRQAGWA
ATLAKHGRPEPPVLIGDWSAESGWRLGTLLARMEEVTAIFAASDDMAFGILRALVDRGRRVPEDVSVVGADDVPLAAFAP
VALTTVRQSFQDLGTAAIDLLVDMVERPDREHHGVLMEPELVLRSSTAPRHAG
>Mature_372_residues
PWASSSPCSVSTTADTCDMSASGFSPPLTGRAPTMADVAKRAAVSPQTVSRVVRGQTNVSEVTRLKVELAIDELGYRRTG
IARALVTGHTMTLGVLTQGSVAYSRAAYVDAMTSAARRHGYYVAAASVTSTAPEAVCQSIEHLRQQGVDGIVVAVPVYDV
DALAQAARGLPAVVFGGSSSEAVPDGFSDGTGHHAVCIDQERIGVLATEHLLGLGHDTVWHVAGPSTWADASGRQAGWAA
TLAKHGRPEPPVLIGDWSAESGWRLGTLLARMEEVTAIFAASDDMAFGILRALVDRGRRVPEDVSVVGADDVPLAAFAPV
ALTTVRQSFQDLGTAAIDLLVDMVERPDREHHGVLMEPELVLRSSTAPRHAG

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786540, Length=314, Percent_Identity=34.3949044585987, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1787948, Length=339, Percent_Identity=32.4483775811209, Blast_Score=143, Evalue=2e-35,
Organism=Escherichia coli, GI1788474, Length=342, Percent_Identity=32.4561403508772, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI1790194, Length=340, Percent_Identity=30, Blast_Score=129, Evalue=3e-31,
Organism=Escherichia coli, GI1789068, Length=323, Percent_Identity=31.2693498452012, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1790369, Length=338, Percent_Identity=28.9940828402367, Blast_Score=117, Evalue=8e-28,
Organism=Escherichia coli, GI1789202, Length=307, Percent_Identity=29.3159609120521, Blast_Score=96, Evalue=3e-21,
Organism=Escherichia coli, GI1789456, Length=321, Percent_Identity=28.6604361370716, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1787580, Length=328, Percent_Identity=27.1341463414634, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1787906, Length=307, Percent_Identity=29.3159609120521, Blast_Score=78, Evalue=9e-16,
Organism=Escherichia coli, GI48994940, Length=286, Percent_Identity=24.4755244755245, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39148; Mature: 39016

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2 ; PS00584 PFKB_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPWASSSPCSVSTTADTCDMSASGFSPPLTGRAPTMADVAKRAAVSPQTVSRVVRGQTNV
CCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCH
SEVTRLKVELAIDELGYRRTGIARALVTGHTMTLGVLTQGSVAYSRAAYVDAMTSAARRH
HHHHHHHHEEEHHHHCCHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHC
GYYVAAASVTSTAPEAVCQSIEHLRQQGVDGIVVAVPVYDVDALAQAARGLPAVVFGGSS
CEEEEECCHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCEEEECCCC
SEAVPDGFSDGTGHHAVCIDQERIGVLATEHLLGLGHDTVWHVAGPSTWADASGRQAGWA
CCCCCCCCCCCCCCEEEEECHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHH
ATLAKHGRPEPPVLIGDWSAESGWRLGTLLARMEEVTAIFAASDDMAFGILRALVDRGRR
HHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCC
VPEDVSVVGADDVPLAAFAPVALTTVRQSFQDLGTAAIDLLVDMVERPDREHHGVLMEPE
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEECCH
LVLRSSTAPRHAG
HHEECCCCCCCCC
>Mature Secondary Structure 
PWASSSPCSVSTTADTCDMSASGFSPPLTGRAPTMADVAKRAAVSPQTVSRVVRGQTNV
CCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHCCCCH
SEVTRLKVELAIDELGYRRTGIARALVTGHTMTLGVLTQGSVAYSRAAYVDAMTSAARRH
HHHHHHHHEEEHHHHCCHHHHHHHHHHHCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHC
GYYVAAASVTSTAPEAVCQSIEHLRQQGVDGIVVAVPVYDVDALAQAARGLPAVVFGGSS
CEEEEECCHHCCCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHCCCCEEEECCCC
SEAVPDGFSDGTGHHAVCIDQERIGVLATEHLLGLGHDTVWHVAGPSTWADASGRQAGWA
CCCCCCCCCCCCCCEEEEECHHCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCHHH
ATLAKHGRPEPPVLIGDWSAESGWRLGTLLARMEEVTAIFAASDDMAFGILRALVDRGRR
HHHHHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCC
VPEDVSVVGADDVPLAAFAPVALTTVRQSFQDLGTAAIDLLVDMVERPDREHHGVLMEPE
CCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCEEECCH
LVLRSSTAPRHAG
HHEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA