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The map label for this gene is btuF [H]

Identifier: 50841567

GI number: 50841567

Start: 82471

End: 83481

Strand: Direct

Name: btuF [H]

Synonym: PPA0076

Alternate gene names: 50841567

Gene position: 82471-83481 (Clockwise)

Preceding gene: 50841566

Following gene: 50841568

Centisome position: 3.22

GC content: 49.36

Gene sequence:

>1011_bases
ATGGTGCGAAAACGCGTGCGAATGACCGCGCTTATTGCCGCGATGGCGATGCCTATATTCAGTCTTGTTGGTTGTGGAAG
TGCTGTCACTGATCCCAGTATTTCGAAAGCATCGACTGCAACGTTGCCGGTGATGCAGGGGCACACTTCGTATCCTCTGA
CTGTCACCAGTTGTGGTGTCAGTCAGGTATTCGAATCTGCTCCTAAGCGTGCCGTAACCTTGACCTCCACCGCGACCGAG
ACGATGCTCGAACTGGGTCTCGAAAAGCGGATGGTTGGAACGGCATATCAACGTAATCGTCCTATTGGATCGCAGTACAA
GAAGGCATACGATTCCATTCCGATTCTCGCCTCCGGGCAACCGTCTATGGAAAAGCTCCTCTCTGTTAACCCAGATTTCG
TTTATTCGGGATATCCGGATGGATTCTCGAAGAGTAATGGACACACTAGAGAGGATCTCAAAAAGCTTGGAATTAAGACC
CATCTGAGTCCGGTGGGGTGCTCTGACCAGGTGAAGAACCTTGAAGATGAAACCAAAGAGATTAGGACGATTGGTCAAAT
CTTCGATGTCAACGATAAGGCGGAAAAGGCTGTTCGCAGGTTCAATTCCAAGGTTAGTAGTGTCAAGAACTCTGTAAAGG
GTAAGGATCGCCCCAAAGTATTCCTGTATAATTCAGGCATAGAAGCTCCGATCACTGTAGGTGGATGGGCCTACGCATCA
GTGATGATCGATGCGGCAGGGGGAGAGAATATTTTTGCCCATGAGCCGCTGAGATGGGGAAAGATGTCGTGGGAGCAAGT
AGCCAAAGCTGATCCAGACATGATTCTCATCTACGACTATAAGACGCCCAGTGTGGATCAGAAGATTAAGACGCTAAAGT
CCATTCCTGCTCTTAAGGATACTAAGGCTGTTAAGAACAACGCCTTCCCTGTCATTAGCCTGTCTTTAGCCCAGCCAGGT
CCCCGGTCCGCCGAGGGTATTGAACAACTTGCTAAACAGTTCCATAGCTGA

Upstream 100 bases:

>100_bases
TTCCCTGTTGGCGCATTCAAGCGAGCGCGAAGAAACTCTTGTTGGACGTGTGTCCTGTGGGCAAGGTCGTCGATGGGCGC
GCGGTTGAAAGGATAATTTG

Downstream 100 bases:

>100_bases
TGCTAGGTGCGCACAGTGAGGGTGACGATACGTCCGTCAGGTCCGTGAGCGACCAGGGTGATCTCGTTGCTATCTTAAAT
CGCGTTCCGGCTCGGTCCTT

Product: periplasmic binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MVRKRVRMTALIAAMAMPIFSLVGCGSAVTDPSISKASTATLPVMQGHTSYPLTVTSCGVSQVFESAPKRAVTLTSTATE
TMLELGLEKRMVGTAYQRNRPIGSQYKKAYDSIPILASGQPSMEKLLSVNPDFVYSGYPDGFSKSNGHTREDLKKLGIKT
HLSPVGCSDQVKNLEDETKEIRTIGQIFDVNDKAEKAVRRFNSKVSSVKNSVKGKDRPKVFLYNSGIEAPITVGGWAYAS
VMIDAAGGENIFAHEPLRWGKMSWEQVAKADPDMILIYDYKTPSVDQKIKTLKSIPALKDTKAVKNNAFPVISLSLAQPG
PRSAEGIEQLAKQFHS

Sequences:

>Translated_336_residues
MVRKRVRMTALIAAMAMPIFSLVGCGSAVTDPSISKASTATLPVMQGHTSYPLTVTSCGVSQVFESAPKRAVTLTSTATE
TMLELGLEKRMVGTAYQRNRPIGSQYKKAYDSIPILASGQPSMEKLLSVNPDFVYSGYPDGFSKSNGHTREDLKKLGIKT
HLSPVGCSDQVKNLEDETKEIRTIGQIFDVNDKAEKAVRRFNSKVSSVKNSVKGKDRPKVFLYNSGIEAPITVGGWAYAS
VMIDAAGGENIFAHEPLRWGKMSWEQVAKADPDMILIYDYKTPSVDQKIKTLKSIPALKDTKAVKNNAFPVISLSLAQPG
PRSAEGIEQLAKQFHS
>Mature_336_residues
MVRKRVRMTALIAAMAMPIFSLVGCGSAVTDPSISKASTATLPVMQGHTSYPLTVTSCGVSQVFESAPKRAVTLTSTATE
TMLELGLEKRMVGTAYQRNRPIGSQYKKAYDSIPILASGQPSMEKLLSVNPDFVYSGYPDGFSKSNGHTREDLKKLGIKT
HLSPVGCSDQVKNLEDETKEIRTIGQIFDVNDKAEKAVRRFNSKVSSVKNSVKGKDRPKVFLYNSGIEAPITVGGWAYAS
VMIDAAGGENIFAHEPLRWGKMSWEQVAKADPDMILIYDYKTPSVDQKIKTLKSIPALKDTKAVKNNAFPVISLSLAQPG
PRSAEGIEQLAKQFHS

Specific function: Part of the ABC transporter complex BtuCDF involved in vitamin B12 import. Binds vitamin B12 and delivers it to the periplasmic surface of BtuC [H]

COG id: COG0614

COG function: function code P; ABC-type Fe3+-hydroxamate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Fe/B12 periplasmic-binding domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002491 [H]

Pfam domain/function: PF01497 Peripla_BP_2 [H]

EC number: NA

Molecular weight: Translated: 36606; Mature: 36606

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50983 FE_B12_PBP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRKRVRMTALIAAMAMPIFSLVGCGSAVTDPSISKASTATLPVMQGHTSYPLTVTSCGV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEECCCH
SQVFESAPKRAVTLTSTATETMLELGLEKRMVGTAYQRNRPIGSQYKKAYDSIPILASGQ
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
PSMEKLLSVNPDFVYSGYPDGFSKSNGHTREDLKKLGIKTHLSPVGCSDQVKNLEDETKE
HHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHH
IRTIGQIFDVNDKAEKAVRRFNSKVSSVKNSVKGKDRPKVFLYNSGIEAPITVGGWAYAS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCCEEEE
VMIDAAGGENIFAHEPLRWGKMSWEQVAKADPDMILIYDYKTPSVDQKIKTLKSIPALKD
EEEECCCCCCCEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHH
TKAVKNNAFPVISLSLAQPGPRSAEGIEQLAKQFHS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MVRKRVRMTALIAAMAMPIFSLVGCGSAVTDPSISKASTATLPVMQGHTSYPLTVTSCGV
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEECCCCCCCEEEECCCH
SQVFESAPKRAVTLTSTATETMLELGLEKRMVGTAYQRNRPIGSQYKKAYDSIPILASGQ
HHHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEECCC
PSMEKLLSVNPDFVYSGYPDGFSKSNGHTREDLKKLGIKTHLSPVGCSDQVKNLEDETKE
HHHHHHHCCCCCEEECCCCCCCCCCCCCCHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHH
IRTIGQIFDVNDKAEKAVRRFNSKVSSVKNSVKGKDRPKVFLYNSGIEAPITVGGWAYAS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCEEECCCEEEE
VMIDAAGGENIFAHEPLRWGKMSWEQVAKADPDMILIYDYKTPSVDQKIKTLKSIPALKD
EEEECCCCCCCEECCCCCCCCCCHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHCCCCHH
TKAVKNNAFPVISLSLAQPGPRSAEGIEQLAKQFHS
HHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]