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The map label for this gene is recF

Identifier: 50841500

GI number: 50841500

Start: 4227

End: 5411

Strand: Direct

Name: recF

Synonym: PPA0004

Alternate gene names: 50841500

Gene position: 4227-5411 (Clockwise)

Preceding gene: 50841499

Following gene: 50841501

Centisome position: 0.17

GC content: 59.58

Gene sequence:

>1185_bases
ATGTTCGTTGAGCACCTCGAACTGGTTGATTTCCGTTCCTATGTCCGTGCCGATGTCCCTATGGCCGCAGGGGCGACGAC
CTTCATTGGCTCTAATGGCCAAGGGAAGACGAACCTCGTTGAGGCGGTGGAATACCTTTCAACGTTGTCGTCGCATCGCG
TCAGCAATGACACCCCGCTGGTGAGGCTGGGAGCCGACCAGGCGGTCGTACGAGGCCGGGTCCGTGCCGGGACCGACGAC
GCCCGCAGCTTGCTGCTGGAGGTCGAGATTAATGCGCGACGTGCCAATCGGGCGCGTATTAATCGAGCTCCGTTAACCCG
TCCGCGGGACATTCTCGGGGTGTTGAGGACTGTGGTGTTTTCGCCTAATGACCTGGCAGTGGTGCGCGGTGATCCGTCGG
ATCGTCGAGCGTTCCTCGATGGCCTAGTGGTGACGCGGTGGCCCAGGATGGCCGCGGTGAAGTCTGATTACGAGCGGGTC
CTCAAGCAGCGCAACGCCTTACTCAAATCCCTGTCCGGGAAGGGGCGATCGGCGGGAGCTGAGATCGGTGCGACGATGGA
CATCTGGGACAACGAGCTGGCTACCATCGGTGCTGAACTACTTTCGGCTCGGCTCGATACTCTGTCGGCTGTCATGCCGT
TGACTTCGGCCGCCTACCGCGAGATTGCCCCAGTGAATGACCTCACCACTGCGTCGTATAAGTCCACGATCGATCTTGAG
GGTCTGTGGTCGCCTCCGCAAGAAAGGGAGAGTTCCACACCGATCGACCGCAAGGAGCTCGCAAACCGATTTCTGGACAC
TTTGGCGAAACGACGTGCTGACGAGCTCATCCGGGGCGTTACCCTCGTCGGCCCGCAACGTGACGACATCATCTTGCATA
TCGGAGAAATGCCGGCTAAAGGGTATGCCTCCCACGGCGAATCATGGTCGTTGGCTCTGGCTTTGAGATTGGGGTCTTTC
CAGCTTCTGCGTGATGACGGTATTGAACCCGTTCTCGTGCTTGATGATGTTTTCGCTGAGCTTGACGCGACGCGTCGAGA
CCGACTGGCATCGTCGGTAGTGCAGGCTGATCAGGTGCTAGTGACGGCCGCGGTAGCCTCGGATGTTCCCGAGATTCTGC
GCGGGGAGAGGTTCGACGTCGGGGGTGGTCAGGTGCTGGCCTATGAGGGGAATAACGATGACTGA

Upstream 100 bases:

>100_bases
GCCACCTGGATGCACCGTCCTACATGGCAGACCGTGGCTGTGAACATGTCTGTCCGGTAACCTGGCTCGGACGGCCTGAA
TGTGGTCGGAGGGGGTCCGG

Downstream 100 bases:

>100_bases
CGGCCTTGACGAAGGTCCGACCGAGGAAGATCGTCGCCTGGCGGCTGATCATGATGACGAGGGTGTCGACGTTGCCCTGG
CTATTGCTCGAGCCGCTCGT

Product: recombination protein F

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MFVEHLELVDFRSYVRADVPMAAGATTFIGSNGQGKTNLVEAVEYLSTLSSHRVSNDTPLVRLGADQAVVRGRVRAGTDD
ARSLLLEVEINARRANRARINRAPLTRPRDILGVLRTVVFSPNDLAVVRGDPSDRRAFLDGLVVTRWPRMAAVKSDYERV
LKQRNALLKSLSGKGRSAGAEIGATMDIWDNELATIGAELLSARLDTLSAVMPLTSAAYREIAPVNDLTTASYKSTIDLE
GLWSPPQERESSTPIDRKELANRFLDTLAKRRADELIRGVTLVGPQRDDIILHIGEMPAKGYASHGESWSLALALRLGSF
QLLRDDGIEPVLVLDDVFAELDATRRDRLASSVVQADQVLVTAAVASDVPEILRGERFDVGGGQVLAYEGNNDD

Sequences:

>Translated_394_residues
MFVEHLELVDFRSYVRADVPMAAGATTFIGSNGQGKTNLVEAVEYLSTLSSHRVSNDTPLVRLGADQAVVRGRVRAGTDD
ARSLLLEVEINARRANRARINRAPLTRPRDILGVLRTVVFSPNDLAVVRGDPSDRRAFLDGLVVTRWPRMAAVKSDYERV
LKQRNALLKSLSGKGRSAGAEIGATMDIWDNELATIGAELLSARLDTLSAVMPLTSAAYREIAPVNDLTTASYKSTIDLE
GLWSPPQERESSTPIDRKELANRFLDTLAKRRADELIRGVTLVGPQRDDIILHIGEMPAKGYASHGESWSLALALRLGSF
QLLRDDGIEPVLVLDDVFAELDATRRDRLASSVVQADQVLVTAAVASDVPEILRGERFDVGGGQVLAYEGNNDD
>Mature_394_residues
MFVEHLELVDFRSYVRADVPMAAGATTFIGSNGQGKTNLVEAVEYLSTLSSHRVSNDTPLVRLGADQAVVRGRVRAGTDD
ARSLLLEVEINARRANRARINRAPLTRPRDILGVLRTVVFSPNDLAVVRGDPSDRRAFLDGLVVTRWPRMAAVKSDYERV
LKQRNALLKSLSGKGRSAGAEIGATMDIWDNELATIGAELLSARLDTLSAVMPLTSAAYREIAPVNDLTTASYKSTIDLE
GLWSPPQERESSTPIDRKELANRFLDTLAKRRADELIRGVTLVGPQRDDIILHIGEMPAKGYASHGESWSLALALRLGSF
QLLRDDGIEPVLVLDDVFAELDATRRDRLASSVVQADQVLVTAAVASDVPEILRGERFDVGGGQVLAYEGNNDD

Specific function: The recF protein is involved in DNA metabolism; it is required for DNA replication and normal SOS inducibility. RecF binds preferentially to single-stranded, linear DNA. It also seems to bind ATP

COG id: COG1195

COG function: function code L; Recombinational DNA repair ATPase (RecF pathway)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recF family

Homologues:

Organism=Escherichia coli, GI1790135, Length=371, Percent_Identity=23.7196765498652, Blast_Score=92, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RECF_PROAC (Q6ABL2)

Other databases:

- EMBL:   AE017283
- RefSeq:   YP_054727.1
- ProteinModelPortal:   Q6ABL2
- GeneID:   2932134
- GenomeReviews:   AE017283_GR
- KEGG:   pac:PPA0004
- NMPDR:   fig|267747.1.peg.4
- HOGENOM:   HBG645124
- OMA:   GQQRTLV
- ProtClustDB:   PRK00064
- BioCyc:   PACN267747:PPA0004-MONOMER
- GO:   GO:0005694
- GO:   GO:0005737
- HAMAP:   MF_00365
- InterPro:   IPR001238
- InterPro:   IPR018078
- InterPro:   IPR003395
- TIGRFAMs:   TIGR00611

Pfam domain/function: PF02463 SMC_N

EC number: NA

Molecular weight: Translated: 43022; Mature: 43022

Theoretical pI: Translated: 5.21; Mature: 5.21

Prosite motif: PS00617 RECF_1; PS00618 RECF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFVEHLELVDFRSYVRADVPMAAGATTFIGSNGQGKTNLVEAVEYLSTLSSHRVSNDTPL
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VRLGADQAVVRGRVRAGTDDARSLLLEVEINARRANRARINRAPLTRPRDILGVLRTVVF
EEECCCHHHHHCCCCCCCHHHHEEEEEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHC
SPNDLAVVRGDPSDRRAFLDGLVVTRWPRMAAVKSDYERVLKQRNALLKSLSGKGRSAGA
CCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EIGATMDIWDNELATIGAELLSARLDTLSAVMPLTSAAYREIAPVNDLTTASYKSTIDLE
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
GLWSPPQERESSTPIDRKELANRFLDTLAKRRADELIRGVTLVGPQRDDIILHIGEMPAK
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEECCCCCC
GYASHGESWSLALALRLGSFQLLRDDGIEPVLVLDDVFAELDATRRDRLASSVVQADQVL
CCCCCCCCEEEEEEEECCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAAVASDVPEILRGERFDVGGGQVLAYEGNNDD
HHHHHHHCCHHHHCCCEEECCCCEEEEECCCCCC
>Mature Secondary Structure
MFVEHLELVDFRSYVRADVPMAAGATTFIGSNGQGKTNLVEAVEYLSTLSSHRVSNDTPL
CCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCE
VRLGADQAVVRGRVRAGTDDARSLLLEVEINARRANRARINRAPLTRPRDILGVLRTVVF
EEECCCHHHHHCCCCCCCHHHHEEEEEEEECCCCCCHHHCCCCCCCCHHHHHHHHHHHHC
SPNDLAVVRGDPSDRRAFLDGLVVTRWPRMAAVKSDYERVLKQRNALLKSLSGKGRSAGA
CCCCEEEEECCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
EIGATMDIWDNELATIGAELLSARLDTLSAVMPLTSAAYREIAPVNDLTTASYKSTIDLE
CCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEC
GLWSPPQERESSTPIDRKELANRFLDTLAKRRADELIRGVTLVGPQRDDIILHIGEMPAK
CCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEECCCCCCEEEEECCCCCC
GYASHGESWSLALALRLGSFQLLRDDGIEPVLVLDDVFAELDATRRDRLASSVVQADQVL
CCCCCCCCEEEEEEEECCCEEEECCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
VTAAVASDVPEILRGERFDVGGGQVLAYEGNNDD
HHHHHHHCCHHHHCCCEEECCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA