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The map label for this gene is bglH

Identifier: 50812306

GI number: 50812306

Start: 4032346

End: 4033755

Strand: Reverse

Name: bglH

Synonym: BSU39260

Alternate gene names: 50812306

Gene position: 4033755-4032346 (Counterclockwise)

Preceding gene: 255767832

Following gene: 16080976

Centisome position: 95.69

GC content: 45.96

Gene sequence:

>1410_bases
ATGAGTTCAAATGAAAAACGATTTCCAGAAGGATTTTTATGGGGCGGCGCAGTTGCTGCCAACCAAGTGGAGGGTGCTTA
TAACGAGGGTGGTAAGGGGCTTTCGACAGCTGACGTATCGCCGAACGGGATCATGTCTCCATTTGATGAATCAATGACTT
CCTTGAATCTGTATCACAACGGGATTGACTTTTATCATCGTTATAAAGAGGATATCGCCTTATTTGCAGAAATGGGATTT
AAGGCGTTCCGCACATCGATTGCGTGGACGAGAATTTTTCCGAATGGCGATGAAGAGGAGCCCAATGAAGAAGGCCTCAG
ATTTTATGATGATCTGTTTGATGAGCTGTTAAAGCATCATATTGAGCCTGTCGTAACCATCTCTCATTATGAGATGCCGC
TCGGTTTAGTGAAAAATTATGGCGGCTGGAAGAATCGCAAAGTCATAGAGTTTTATGAACGCTATGCCAAAACGGTTTTC
AAACGCTATCAACACAAAGTGAAGTATTGGATGACGTTTAATGAAATTAACGTAGTGCTCCATGCGCCATTTACCGGAGG
CGGTCTTGTTTTTGAAGAAGGAGAAAACAAATTAAACGCGATGTACCAGGCGGCACACCACCAATTTGTGGCCAGTGCTC
TTGCTGTCAAAGCGGGTCACGACATCATTCCTGATTCGAAAATCGGCTGTATGATAGCGGCAACGACAACCTATCCGATG
ACTTCTAAGCCGGAGGATGTCTTCGCGGCAATGGAGAATGAGCGCAAAACACTTTTCTTCTCAGATGTTCAGGCAAGAGG
CGCATACCCGGGATATATGAAACGCTATCTGGCAGAAAACAATATTGAAATTGAAATGGCTGAAGGCGATGAAGAGCTCT
TGAAAGAACATACGGTGGATTATATTGGATTTAGCTACTACATGTCGATGGCGGCGAGCACAGATCCGGAAGAGCTTGCA
AAATCAGGGGGCAACCTGCTGGGCGGAGTCAAAAACCCTTACCTCAAATCATCAGAATGGGGCTGGCAGATTGATCCGAA
AGGCTTGCGCATCACGCTCAACACCTTATACGACCGTTACCAGAAGCCGCTGTTCATCGTTGAAAACGGCCTTGGCGCTG
TAGACAAGGTTGAAGAGGACGGCACGATTCAGGACGATTACAGAATCAACTATCTGCGTGATCATTTAATTGAAGCGCGC
GAAGCCATTGCGGACGGCGTTGAATTAATCGGATACACATCATGGGGGCCAATTGACCTTGTCAGCGCATCTACTGCGGA
AATGAAAAAGCGCTACGGCTTCATTTATGTAGACCGGGACAATGAAGGAAATGGAACATTTAACCGCATCAAGAAAAAAA
GCTTCAACTGGTATCAGCAGGTTATCGCCACAAACGGAGAGAGTCTCTGA

Upstream 100 bases:

>100_bases
ACAGACCAATATTCGTTTTCGCCGGTGAAAGAGATCGGTAAAGTGCAGCCAAAAGAAGCGCTGCTTGCTTTATCTTGAAA
AAACTAATGGGGTGATCAAT

Downstream 100 bases:

>100_bases
CAATTTTTTGAAAACTCATGCGCTTCAATTGACAATAACGAAATGCAGGCGGACAATAAAAGAGAAAGATGAACCACCCA
CAGTCCGAAAGGCGGGAAGA

Product: aryl-phospho-beta-d-glucosidase

Products: NA

Alternate protein names: 6-phospho-beta-glucosidase

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHNGIDFYHRYKEDIALFAEMGF
KAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHHIEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVF
KRYQHKVKYWMTFNEINVVLHAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPM
TSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVDYIGFSYYMSMAASTDPEELA
KSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRYQKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAR
EAIADGVELIGYTSWGPIDLVSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL

Sequences:

>Translated_469_residues
MSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHNGIDFYHRYKEDIALFAEMGF
KAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHHIEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVF
KRYQHKVKYWMTFNEINVVLHAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPM
TSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVDYIGFSYYMSMAASTDPEELA
KSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRYQKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAR
EAIADGVELIGYTSWGPIDLVSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL
>Mature_468_residues
SSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHNGIDFYHRYKEDIALFAEMGFK
AFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHHIEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFK
RYQHKVKYWMTFNEINVVLHAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPMT
SKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVDYIGFSYYMSMAASTDPEELAK
SGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRYQKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEARE
AIADGVELIGYTSWGPIDLVSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL

Specific function: Catalyzes the hydrolysis of aryl-phospho-beta-D- glucosides such as 4-methylumbelliferyl-phospho-beta-D- glucopyranoside (MUG-P), phosphoarbutin and phosphosalicin. Plays a major role in the utilization of arbutin or salicin as the sole carbon source. Bgl

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family

Homologues:

Organism=Homo sapiens, GI32481206, Length=479, Percent_Identity=30.062630480167, Blast_Score=192, Evalue=5e-49,
Organism=Homo sapiens, GI110681710, Length=484, Percent_Identity=29.5454545454545, Blast_Score=177, Evalue=2e-44,
Organism=Homo sapiens, GI28376633, Length=485, Percent_Identity=28.0412371134021, Blast_Score=152, Evalue=8e-37,
Organism=Homo sapiens, GI13273313, Length=482, Percent_Identity=26.3485477178423, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI24497614, Length=478, Percent_Identity=28.2426778242678, Blast_Score=140, Evalue=2e-33,
Organism=Escherichia coli, GI2367270, Length=465, Percent_Identity=63.6559139784946, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI1789070, Length=471, Percent_Identity=57.3248407643312, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI2367174, Length=473, Percent_Identity=56.6596194503171, Blast_Score=542, Evalue=1e-155,
Organism=Caenorhabditis elegans, GI17552856, Length=483, Percent_Identity=30.0207039337474, Blast_Score=187, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17539390, Length=481, Percent_Identity=30.1455301455301, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI21356577, Length=497, Percent_Identity=26.5593561368209, Blast_Score=148, Evalue=6e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BGLH_BACSU (P40740)

Other databases:

- EMBL:   Z34526
- EMBL:   D31856
- EMBL:   D29985
- EMBL:   AL009126
- PIR:   H69593
- RefSeq:   NP_391805.2
- ProteinModelPortal:   P40740
- SMR:   P40740
- EnsemblBacteria:   EBBACT00000004016
- GeneID:   937512
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU39260
- NMPDR:   fig|224308.1.peg.3931
- GenoList:   BSU39260
- GeneTree:   EBGT00050000000632
- HOGENOM:   HBG316462
- OMA:   FKCLRTS
- PhylomeDB:   P40740
- ProtClustDB:   CLSK928157
- BioCyc:   BSUB:BSU39260-MONOMER
- BRENDA:   3.2.1.21
- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781
- Gene3D:   G3DSA:3.20.20.80
- PANTHER:   PTHR10353
- PRINTS:   PR00131

Pfam domain/function: PF00232 Glyco_hydro_1; SSF51445 Glyco_hydro_cat

EC number: =3.2.1.86

Molecular weight: Translated: 53289; Mature: 53158

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: ACT_SITE 175-175 ACT_SITE 368-368

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHN
CCCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHC
GIDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHH
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHC
IEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFKRYQHKVKYWMTFNEINVVL
CCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEE
HAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPM
ECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
TSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVD
CCCCHHHHHHHHCCCCEEEEECHHHCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
YIGFSYYMSMAASTDPEELAKSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRY
HHHHHHHHHHHCCCCHHHHHHCCCCEECCCCCCCCCCCCCCEEECCCCEEEEHHHHHHHH
QKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAREAIADGVELIGYTSWGPIDL
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEE
VSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL
ECCCHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SSNEKRFPEGFLWGGAVAANQVEGAYNEGGKGLSTADVSPNGIMSPFDESMTSLNLYHN
CCCCCCCCCCEEECCHHHHHHCCCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHC
GIDFYHRYKEDIALFAEMGFKAFRTSIAWTRIFPNGDEEEPNEEGLRFYDDLFDELLKHH
CHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHC
IEPVVTISHYEMPLGLVKNYGGWKNRKVIEFYERYAKTVFKRYQHKVKYWMTFNEINVVL
CCCEEEEECCCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEEEEEEEEE
HAPFTGGGLVFEEGENKLNAMYQAAHHQFVASALAVKAGHDIIPDSKIGCMIAATTTYPM
ECCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCC
TSKPEDVFAAMENERKTLFFSDVQARGAYPGYMKRYLAENNIEIEMAEGDEELLKEHTVD
CCCCHHHHHHHHCCCCEEEEECHHHCCCCHHHHHHHHCCCCCEEEECCCHHHHHHHHHHH
YIGFSYYMSMAASTDPEELAKSGGNLLGGVKNPYLKSSEWGWQIDPKGLRITLNTLYDRY
HHHHHHHHHHHCCCCHHHHHHCCCCEECCCCCCCCCCCCCCEEECCCCEEEEHHHHHHHH
QKPLFIVENGLGAVDKVEEDGTIQDDYRINYLRDHLIEAREAIADGVELIGYTSWGPIDL
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHEEEECCCCCEEE
VSASTAEMKKRYGFIYVDRDNEGNGTFNRIKKKSFNWYQQVIATNGESL
ECCCHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7883710; 7704263; 9384377