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The map label for this gene is ruvB

Identifier: 50812277

GI number: 50812277

Start: 2835150

End: 2836154

Strand: Reverse

Name: ruvB

Synonym: BSU27730

Alternate gene names: 50812277

Gene position: 2836154-2835150 (Counterclockwise)

Preceding gene: 16079826

Following gene: 255767633

Centisome position: 67.28

GC content: 50.15

Gene sequence:

>1005_bases
ATGGATGAACGGCTCGTCTCAAGTGAAGCAGACAACCATGAATCAGTGATAGAGCAAAGCCTGAGGCCGCAAAATCTGGC
ACAGTATATCGGGCAGCATAAGGTAAAAGAAAATTTGCGCGTGTTTATAGATGCGGCGAAAATGAGACAGGAGACGCTGG
ATCATGTTCTTCTGTACGGGCCGCCCGGACTCGGAAAAACAACGCTTGCTTCGATTGTTGCTAATGAAATGGGAGTGGAA
TTGCGGACGACATCAGGGCCTGCGATTGAAAGGCCTGGTGATTTGGCTGCTATATTAACGGCGCTGGAACCGGGAGATGT
ATTATTTATTGATGAAATACATAGGCTGCACAGATCGATCGAAGAGGTGCTTTATCCTGCAATGGAAGACTTTTGCCTGG
ACATTGTGATTGGCAAAGGCCCATCTGCCCGCTCGGTTCGCCTCGACCTTCCGCCTTTTACGCTGGTGGGAGCCACGACG
AGGGTGGGGCTTTTGACGGCTCCTTTAAGAGACAGATTCGGCGTAATGTCTCGCCTTGAGTACTATACACAAGAAGAACT
GGCGGATATTGTCACAAGAACCGCTGATGTATTCGAGGTTGAGATTGATAAACCGTCGGCTCTTGAAATTGCAAGAAGAT
CGAGAGGGACGCCCCGCGTGGCGAACCGCCTGCTGAGAAGGGTGCGCGATTTCGCCCAAGTGCTGGGTGACAGCAGGATT
ACGGAAGACATTTCTCAGAATGCGCTGGAGCGGCTTCAGGTTGACCGCCTCGGTTTGGATCATATTGATCATAAGCTGCT
GATGGGCATGATCGAAAAATTCAACGGCGGCCCAGTAGGCCTTGATACGATTTCAGCTACCATCGGCGAGGAATCGCATA
CGATTGAAGACGTATATGAACCATATCTGCTGCAAATTGGATTTATACAAAGAACGCCGCGTGGAAGGATCGTGACACCG
GCCGTTTATCATCATTTCCAAATGGAGGCTCCCCGATATGACTGA

Upstream 100 bases:

>100_bases
ATCAAAAAGGTGCTTCCTCACCTTAAAGAAGAAATTGGGCTGACAACAGACCAATATGTGAAAAAAGCATTACAAAAACT
ATTAAAGTAAGGTGATGTTC

Downstream 100 bases:

>100_bases
ATTTCCTAAGATCATTATGATTCTTGGTGCTGTTTTGCTTATTATCGGAGCAGTTTTGCATTTTGTCGGCAAGATGCCGG
GAGATATTTTTGTAAAAAAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Sequences:

>Translated_334_residues
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD
>Mature_334_residues
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYGPPGLGKTTLASIVANEMGVE
LRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSIEEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATT
RVGLLTAPLRDRFGVMSRLEYYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYEPYLLQIGFIQRTPRGRIVTP
AVYHHFQMEAPRYD

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family [H]

Homologues:

Organism=Escherichia coli, GI1788167, Length=329, Percent_Identity=60.4863221884498, Blast_Score=416, Evalue=1e-117,
Organism=Escherichia coli, GI1787119, Length=136, Percent_Identity=32.3529411764706, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- ProDom:   PD005323 [H]

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 37468; Mature: 37468

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYG
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
PPGLGKTTLASIVANEMGVELRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSI
CCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRVGLLTAPLRDRFGVMSRLE
HHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCHHHHHHEECCHHHHHHHHHHHH
YYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHH
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHH
PYLLQIGFIQRTPRGRIVTPAVYHHFQMEAPRYD
HHHHHHHHHEECCCCCEECHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDERLVSSEADNHESVIEQSLRPQNLAQYIGQHKVKENLRVFIDAAKMRQETLDHVLLYG
CCCCCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
PPGLGKTTLASIVANEMGVELRTTSGPAIERPGDLAAILTALEPGDVLFIDEIHRLHRSI
CCCCCHHHHHHHHHHHHCCEEEECCCCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHHHHH
EEVLYPAMEDFCLDIVIGKGPSARSVRLDLPPFTLVGATTRVGLLTAPLRDRFGVMSRLE
HHHHHHHHHHHEEEEEECCCCCCCEEEECCCCEEEECCHHHHHHEECCHHHHHHHHHHHH
YYTQEELADIVTRTADVFEVEIDKPSALEIARRSRGTPRVANRLLRRVRDFAQVLGDSRI
HHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHH
TEDISQNALERLQVDRLGLDHIDHKLLMGMIEKFNGGPVGLDTISATIGEESHTIEDVYE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHH
PYLLQIGFIQRTPRGRIVTPAVYHHFQMEAPRYD
HHHHHHHHHEECCCCCEECHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA