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The map label for this gene is fabI

Identifier: 50812220

GI number: 50812220

Start: 1247788

End: 1248564

Strand: Direct

Name: fabI

Synonym: BSU11720

Alternate gene names: 50812220

Gene position: 1247788-1248564 (Clockwise)

Preceding gene: 16078236

Following gene: 16078238

Centisome position: 29.6

GC content: 48.13

Gene sequence:

>777_bases
ATGAATTTTTCACTTGAAGGCCGTAACATTGTTGTGATGGGGGTAGCCAACAAACGCAGCATCGCCTGGGGCATTGCGCG
TTCTTTACATGAAGCGGGTGCACGTTTGATTTTCACATACGCTGGTGAACGCCTGGAGAAATCCGTTCACGAGCTTGCCG
GAACATTAGACCGCAACGATTCCATCATCCTCCCTTGCGATGTTACAAACGACGCAGAAATCGAAACTTGCTTCGCAAGC
ATTAAGGAGCAGGTCGGTGTAATCCACGGTATCGCGCATTGTATCGCGTTTGCCAACAAAGAAGAGCTTGTCGGCGAGTA
CTTAAACACAAATCGTGACGGCTTCCTTTTGGCTCATAACATCAGCTCATATTCTCTGACTGCTGTTGTCAAAGCGGCAC
GTCCGATGATGACTGAAGGCGGAAGCATTGTCACTTTGACGTACCTTGGCGGAGAGCTTGTGATGCCAAACTACAACGTC
ATGGGTGTAGCAAAAGCTTCTCTTGATGCAAGTGTGAAATATTTAGCTGCTGACTTAGGAAAAGAAAATATCCGCGTCAA
CAGCATTTCTGCCGGCCCGATCAGAACATTATCTGCTAAAGGCATCAGCGATTTCAACTCTATCTTAAAAGACATCGAAG
AGCGTGCACCGCTTCGCCGCACGACAACACCTGAAGAAGTGGGCGATACAGCTGCGTTCTTGTTCAGCGATATGTCCCGC
GGGATTACAGGTGAAAATCTTCACGTTGATTCTGGTTTCCATATCACTGCCCGCTAA

Upstream 100 bases:

>100_bases
TACGGTATGCGCTTTTCATCTGGACAAGGTTATGATATGATAGGAATTAAGATCAGGTACTAATACTATGTCTTTATTAA
ATATAGGAGGCTTTATACAT

Downstream 100 bases:

>100_bases
GCAGGATCTATATCAAGCAATCCCGAATAGGGATTGCTTTTTTATTTCTTCTGATTTTCAGCTTTCTGTCATATAGATAG
AATATGACACAATCGGAGGG

Product: enoyl-(acyl carrier protein) reductase

Products: NA

Alternate protein names: Cold shock-induced protein 15; CSI15; NADH-dependent enoyl-ACP reductase; Vegetative protein 241; VEG241

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MNFSLEGRNIVVMGVANKRSIAWGIARSLHEAGARLIFTYAGERLEKSVHELAGTLDRNDSIILPCDVTNDAEIETCFAS
IKEQVGVIHGIAHCIAFANKEELVGEYLNTNRDGFLLAHNISSYSLTAVVKAARPMMTEGGSIVTLTYLGGELVMPNYNV
MGVAKASLDASVKYLAADLGKENIRVNSISAGPIRTLSAKGISDFNSILKDIEERAPLRRTTTPEEVGDTAAFLFSDMSR
GITGENLHVDSGFHITAR

Sequences:

>Translated_258_residues
MNFSLEGRNIVVMGVANKRSIAWGIARSLHEAGARLIFTYAGERLEKSVHELAGTLDRNDSIILPCDVTNDAEIETCFAS
IKEQVGVIHGIAHCIAFANKEELVGEYLNTNRDGFLLAHNISSYSLTAVVKAARPMMTEGGSIVTLTYLGGELVMPNYNV
MGVAKASLDASVKYLAADLGKENIRVNSISAGPIRTLSAKGISDFNSILKDIEERAPLRRTTTPEEVGDTAAFLFSDMSR
GITGENLHVDSGFHITAR
>Mature_258_residues
MNFSLEGRNIVVMGVANKRSIAWGIARSLHEAGARLIFTYAGERLEKSVHELAGTLDRNDSIILPCDVTNDAEIETCFAS
IKEQVGVIHGIAHCIAFANKEELVGEYLNTNRDGFLLAHNISSYSLTAVVKAARPMMTEGGSIVTLTYLGGELVMPNYNV
MGVAKASLDASVKYLAADLGKENIRVNSISAGPIRTLSAKGISDFNSILKDIEERAPLRRTTTPEEVGDTAAFLFSDMSR
GITGENLHVDSGFHITAR

Specific function: Fatty acid biosynthesis pathway; second reduction step. [C]

COG id: COG0623

COG function: function code I; Enoyl-[acyl-carrier-protein] reductase (NADH)

Gene ontology:

Cell location: Inner Membrane-Associated [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family. FabI subfamily

Homologues:

Organism=Escherichia coli, GI1787545, Length=258, Percent_Identity=51.5503875968992, Blast_Score=260, Evalue=6e-71,
Organism=Escherichia coli, GI87082100, Length=257, Percent_Identity=29.5719844357977, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787335, Length=262, Percent_Identity=28.2442748091603, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1790717, Length=253, Percent_Identity=23.3201581027668, Blast_Score=65, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI115534694, Length=261, Percent_Identity=27.2030651340996, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): FABI_BACSU (P54616)

Other databases:

- EMBL:   AL009126
- PIR:   G69845
- RefSeq:   NP_389054.2
- ProteinModelPortal:   P54616
- SMR:   P54616
- EnsemblBacteria:   EBBACT00000002161
- GeneID:   939379
- GenomeReviews:   AL009126_GR
- KEGG:   bsu:BSU11720
- NMPDR:   fig|224308.1.peg.1173
- GenoList:   BSU11720
- GeneTree:   EBGT00050000000469
- HOGENOM:   HBG750976
- OMA:   LVHCLAF
- PhylomeDB:   P54616
- ProtClustDB:   PRK08594
- BioCyc:   BSUB:BSU11720-MONOMER
- BRENDA:   1.3.1.9
- GO:   GO:0005488
- InterPro:   IPR002198
- InterPro:   IPR014358
- InterPro:   IPR002347
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.40.50.720
- PANTHER:   PTHR19410
- PANTHER:   PTHR19410:SF12
- PIRSF:   PIRSF000094
- PRINTS:   PR00081

Pfam domain/function: PF00106 adh_short

EC number: =1.3.1.9

Molecular weight: Translated: 27874; Mature: 27874

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: ACT_SITE 158-158

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFSLEGRNIVVMGVANKRSIAWGIARSLHEAGARLIFTYAGERLEKSVHELAGTLDRND
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
SIILPCDVTNDAEIETCFASIKEQVGVIHGIAHCIAFANKEELVGEYLNTNRDGFLLAHN
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEC
ISSYSLTAVVKAARPMMTEGGSIVTLTYLGGELVMPNYNVMGVAKASLDASVKYLAADLG
CCCHHHHHHHHHHCCHHCCCCCEEEEEEECCEEECCCCCEEEEECCCHHHHHHHHHHHCC
KENIRVNSISAGPIRTLSAKGISDFNSILKDIEERAPLRRTTTPEEVGDTAAFLFSDMSR
CCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
GITGENLHVDSGFHITAR
CCCCCCEEECCCEEEEEC
>Mature Secondary Structure
MNFSLEGRNIVVMGVANKRSIAWGIARSLHEAGARLIFTYAGERLEKSVHELAGTLDRND
CCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHCCCCC
SIILPCDVTNDAEIETCFASIKEQVGVIHGIAHCIAFANKEELVGEYLNTNRDGFLLAHN
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCEEEEEC
ISSYSLTAVVKAARPMMTEGGSIVTLTYLGGELVMPNYNVMGVAKASLDASVKYLAADLG
CCCHHHHHHHHHHCCHHCCCCCEEEEEEECCEEECCCCCEEEEECCCHHHHHHHHHHHCC
KENIRVNSISAGPIRTLSAKGISDFNSILKDIEERAPLRRTTTPEEVGDTAAFLFSDMSR
CCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHC
GITGENLHVDSGFHITAR
CCCCCCEEECCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377; 8755892; 9298659