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The map label for this gene is fba [H]

Identifier: 50365459

GI number: 50365459

Start: 749713

End: 750603

Strand: Reverse

Name: fba [H]

Synonym: Mfl644

Alternate gene names: 50365459

Gene position: 750603-749713 (Counterclockwise)

Preceding gene: 50365460

Following gene: 50365458

Centisome position: 94.63

GC content: 33.78

Gene sequence:

>891_bases
ATGACAAGACTATATCATGAAAAATTAGTTAATGCTACAGCTATGGTTAAAGCAGCACACGAAAACAAATACGCAATCGG
TCACTTTAACATTAATAACTTAGAATGAACTAAAGCAATCTTAGAAGCAGCTCAAGCTTCAAACACACCTGTTATCTTAG
GAACAAGTGAAGGTGCTATTAAATATATGGGTGGACCAGTTACTGTTGTTGGGATGGTTAATGGTTTATTAGAAGAATTA
AACATTACTGTGCCAGTTGCATTACACTTAGACCACGGACAATCAATTGAAATGGCTAAAAAATGTATTTTAGCTGGTTA
CTCATCAGTTATGTTTGATGGATCACACTTACCATACGAAGAAAACTTAGCAAAAACTAAAGAATTATTAATTTTTGCTA
ATGAACACGAAATTTCAGTTGAAGCTGAAATTGGTTCAATTGGTGGAGAAGAAGATGGAGTTGTTGGAAACGGTGAATTA
GGTGACCCAACTCAAGCAGCAGAAATTTCAAAAACAGGAATTGCAATGTTAGCAGCAGGAATCGGAAACATTCACGGAAA
ATACCCTGAATGATGAACAGGATTATCATTTGATACTTTAGAAGAATTACAAGCAGCATGTAAAATGCCAATGGTATTAC
ATGGTGGAAGTGGAATTCCTCAAGACCAAGTTGAAAAAGCTATTTCAATGGGGATTTCAAAAATCAACGTTAATACTGAA
TTACAATTAGCATTTAGAGATGCAACAAGAAAATATATTGAAGCTGGAAAAGACTTAGATGACAAAACAAAAGGTTTTGA
TCCAAGAAAATTATTAAACCCAGGATACCAAGCATTAAAAGAAACATTTAACGAATTAACAGAATGATTCGGATGTAAAG
GTAAAGCTTAA

Upstream 100 bases:

>100_bases
TTTACATTTAAATGAACAGCAAAAAGATAATATAAAATTATTTGTTTAAAAGTTATAAAGTTGTATAATTTTAAAGAAGT
AAAAGAAAGAGGATAAATAA

Downstream 100 bases:

>100_bases
TATTTAATCAAATAATTAAAAAATCGCGAAAGCGGTTTTTTTTTAAATTTTTCACTCCATAAAAAAACAGATTATTATAT
ATTTTTGGAGTAAATTTCCA

Product: fructose-biphosphate aldolase

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 296; Mature: 295

Protein sequence:

>296_residues
MTRLYHEKLVNATAMVKAAHENKYAIGHFNINNLEWTKAILEAAQASNTPVILGTSEGAIKYMGGPVTVVGMVNGLLEEL
NITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEENLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGEL
GDPTQAAEISKTGIAMLAAGIGNIHGKYPEWWTGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTE
LQLAFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTEWFGCKGKA

Sequences:

>Translated_296_residues
MTRLYHEKLVNATAMVKAAHENKYAIGHFNINNLE*TKAILEAAQASNTPVILGTSEGAIKYMGGPVTVVGMVNGLLEEL
NITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEENLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGEL
GDPTQAAEISKTGIAMLAAGIGNIHGKYPE**TGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTE
LQLAFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTE*FGCKGKA
>Mature_295_residues
TRLYHEKLVNATAMVKAAHENKYAIGHFNINNLE*TKAILEAAQASNTPVILGTSEGAIKYMGGPVTVVGMVNGLLEELN
ITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEENLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGELG
DPTQAAEISKTGIAMLAAGIGNIHGKYPE**TGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTEL
QLAFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTE*FGCKGKA

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI87082041, Length=291, Percent_Identity=34.3642611683849, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1789526, Length=279, Percent_Identity=35.1254480286738, Blast_Score=171, Evalue=6e-44,
Organism=Escherichia coli, GI1788072, Length=224, Percent_Identity=38.8392857142857, Blast_Score=138, Evalue=4e-34,
Organism=Escherichia coli, GI1789293, Length=335, Percent_Identity=26.2686567164179, Blast_Score=78, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6322790, Length=324, Percent_Identity=28.3950617283951, Blast_Score=86, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 31244; Mature: 31113

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS00806 ALDOLASE_CLASS_II_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRLYHEKLVNATAMVKAAHENKYAIGHFNINNLETKAILEAAQASNTPVILGTSEGAIK
CCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCEE
YMGGPVTVVGMVNGLLEELNITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEE
ECCCCEEHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCHHHEECCCCCCHHH
NLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGELGDPTQAAEISKTGIAMLAAGI
HHHHCEEEEEEECCCCEEEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHH
GNIHGKYPETGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTELQL
HCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCHHEEECCCEEEE
AFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTEFGCKGKA
EEHHHHHHHHHCCCCCCHHHCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TRLYHEKLVNATAMVKAAHENKYAIGHFNINNLETKAILEAAQASNTPVILGTSEGAIK
CCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHCCCCCEEEECCCCCEE
YMGGPVTVVGMVNGLLEELNITVPVALHLDHGQSIEMAKKCILAGYSSVMFDGSHLPYEE
ECCCCEEHHHHHHHHHHHCCCEEEEEEEECCCCCHHHHHHHHHHCCHHHEECCCCCCHHH
NLAKTKELLIFANEHEISVEAEIGSIGGEEDGVVGNGELGDPTQAAEISKTGIAMLAAGI
HHHHCEEEEEEECCCCEEEEEEECCCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHH
GNIHGKYPETGLSFDTLEELQAACKMPMVLHGGSGIPQDQVEKAISMGISKINVNTELQL
HCCCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCHHEEECCCEEEE
AFRDATRKYIEAGKDLDDKTKGFDPRKLLNPGYQALKETFNELTEFGCKGKA
EEHHHHHHHHHCCCCCCHHHCCCCCHHHCCHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]