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The map label for this gene is pheT [H]

Identifier: 50365406

GI number: 50365406

Start: 689297

End: 689878

Strand: Reverse

Name: pheT [H]

Synonym: Mfl589

Alternate gene names: 50365406

Gene position: 689878-689297 (Counterclockwise)

Preceding gene: 50365409

Following gene: 50365405

Centisome position: 86.97

GC content: 26.46

Gene sequence:

>582_bases
ATGAGTACAAAATTTGGAGTATTTTACAATAAACAGTTTAATTGTTTAATGTTAACTTTTAAAAACAAACCTATTACATC
TTCAGAAAAAAAAGGGAATATAGTTATTTTAAAAAATGAGGAGGAGTTAGTAGGGATAAATATCTTTGAAGTAAAATTAG
AGATTAATAAAAATTTTATTTATAATGATTCTGATATTTTAACTTATATTAATTCTGAGTTAAAGGATGTTATTAAACTT
GAGCCATTAGAACAATTTGTTATTGGGAAAATTATCAAATGTGATGCAATCCCTGACACACACTTGAGTGCTTGTGAAGT
TGATATTAAAACAAAAATTTTAAAAATAGTTTGTGGTGCAAAAAATGCTAGAGAAGGTATATTTGTAGTTGTTGCAACAG
ATGGAAGTTGAATGCCTAATGGAATGCAAATTCAAAAAGGTAAGTTAAGAGGATATGATTCAGAAGGAATGTTATGTAGC
GCAAAAGAATTAAATATTGAAGAAGGAAAATTTAATTCTGAAGGCATAATTGAGTTAGATGAAAAATATCGCAATAATTT
AGGTAGCGAGTTTAAAGGTTAA

Upstream 100 bases:

>100_bases
TCAGTTAAAATAGCTATTTTCATAATATAACCTCCTGTTGTGCTTTTTTAATAACTTAATTATATAATATTAATAAAATT
ATTAAAATAGAGGTAGACAC

Downstream 100 bases:

>100_bases
TTTTATGAATAAAATAAATTTTGATAAAAAAATAAAAGACGGATATTATATAGCTGACTATTTTAAAAAAACTAGTTTAA
TTATTAATAACGAATTTCCA

Product: phenylalanyl-tRNA synthetase, beta subunit

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MSTKFGVFYNKQFNCLMLTFKNKPITSSEKKGNIVILKNEEELVGINIFEVKLEINKNFIYNDSDILTYINSELKDVIKL
EPLEQFVIGKIIKCDAIPDTHLSACEVDIKTKILKIVCGAKNAREGIFVVVATDGSWMPNGMQIQKGKLRGYDSEGMLCS
AKELNIEEGKFNSEGIIELDEKYRNNLGSEFKG

Sequences:

>Translated_193_residues
MSTKFGVFYNKQFNCLMLTFKNKPITSSEKKGNIVILKNEEELVGINIFEVKLEINKNFIYNDSDILTYINSELKDVIKL
EPLEQFVIGKIIKCDAIPDTHLSACEVDIKTKILKIVCGAKNAREGIFVVVATDGS*MPNGMQIQKGKLRGYDSEGMLCS
AKELNIEEGKFNSEGIIELDEKYRNNLGSEFKG
>Mature_192_residues
STKFGVFYNKQFNCLMLTFKNKPITSSEKKGNIVILKNEEELVGINIFEVKLEINKNFIYNDSDILTYINSELKDVIKLE
PLEQFVIGKIIKCDAIPDTHLSACEVDIKTKILKIVCGAKNAREGIFVVVATDGS*MPNGMQIQKGKLRGYDSEGMLCSA
KELNIEEGKFNSEGIIELDEKYRNNLGSEFKG

Specific function: Unknown

COG id: COG0073

COG function: function code R; EMAP domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=68, Percent_Identity=51.4705882352941, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 21567; Mature: 21436

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKFGVFYNKQFNCLMLTFKNKPITSSEKKGNIVILKNEEELVGINIFEVKLEINKNFI
CCCEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCEE
YNDSDILTYINSELKDVIKLEPLEQFVIGKIIKCDAIPDTHLSACEVDIKTKILKIVCGA
ECCCHHEEHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCCCCEEEEHHHHEEEEEEECC
KNAREGIFVVVATDGSMPNGMQIQKGKLRGYDSEGMLCSAKELNIEEGKFNSEGIIELDE
CCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEHH
KYRNNLGSEFKG
HHHCCCCCCCCC
>Mature Secondary Structure 
STKFGVFYNKQFNCLMLTFKNKPITSSEKKGNIVILKNEEELVGINIFEVKLEINKNFI
CCEEEEEECCCCEEEEEEECCCCCCCCCCCCCEEEEECCCCEEEEEEEEEEEEECCCEE
YNDSDILTYINSELKDVIKLEPLEQFVIGKIIKCDAIPDTHLSACEVDIKTKILKIVCGA
ECCCHHEEHHHHHHHHHHCCCCHHHHHHCCEEEECCCCCCCCCEEEEHHHHEEEEEEECC
KNAREGIFVVVATDGSMPNGMQIQKGKLRGYDSEGMLCSAKELNIEEGKFNSEGIIELDE
CCCCCCEEEEEEECCCCCCCCEEECCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEEEHH
KYRNNLGSEFKG
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA