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The map label for this gene is tyrS [H]

Identifier: 50365404

GI number: 50365404

Start: 686985

End: 688226

Strand: Reverse

Name: tyrS [H]

Synonym: Mfl587

Alternate gene names: 50365404

Gene position: 688226-686985 (Counterclockwise)

Preceding gene: 50365405

Following gene: 50365402

Centisome position: 86.76

GC content: 28.34

Gene sequence:

>1242_bases
ATGAATATAATTAAAGAACTAGAATGACGTGGGTTGGTAAAACAAATCACAAATGAAGAAAGACTATTAAAAGCACAAAA
TGATGGAGCTGCTGTTTATTGTGGTTTTGACCCAACTGCAGATTCATTGCATGTTGGTCATTTAATGATGATAGTTACAC
TTAAAAGATTTGATAAAGCTGGATTTCAAGCGATTGGATTAATTGGTGGTGGTACTGGAATGATAGGGGACCCATCATTT
AAAGCTGATGAAAGAAAATTACAAACAGATGAACAAGTTAAATTTCATGCAACAGCAATACAAAATCAATTATTAAGAAT
AATTCCTGATGTTACATTTGCTAATAATGTTGATTGATTAGGTAATATGTCTTTAATTGATTTTTTAAGAGATGTTGGAA
AAGATTTCAATATTAGTTATTTACTAAATAAAGACTCAATTGCCACTAGAATAAGCACAGGATTATCTGTGACAGAGTTT
TCTTATACAATGCTTCAAGCTTATGATTTTTACAATCTATATATAAATCATAATTGTAAAGTTCAAATTGGTGGAAGCGA
TCAATGAGGAAATATAACAAGTGGAACAGATTACATTTCAACAAGAGTAGGTTCTGCTAACACTGAAGCAGCAGGATTTA
CAATACCACTTTTAACTAAATCAGATGGTCAAAAATTTGGTAAAACAGAATCAGGAGCAGTTTGATTAGATTCAAATAAA
ACTAGTGTTTATGATTTTTACCAATTTTGAATTAACCAAGACGACAATGATTGTGTGAAAATGCTAAAATATTTAACATT
TTTAACAAAAGAAGAAATTGATGAATTAGAAGCAAAACATAAAGAAGCTCCTCACTTAAGAACAATGCAGAAAACACTTG
CTTCTGAAATTACAAAATTTGTTCATGGTGATAAAGAATTGAACAAAGCTATTAAATTAACTGATGCGTTTTTTACTGGA
AATATTTTAAATCTAGATGATGATCTTTTAGAGTTAGCAATTAAATCAATACCAACTATTCAATTAGAAAAAACAACTAG
TGCAATTGATGCAATAGTTAATGTAAATGCAGCATCATCAAAAAGAGAGGCTAGAGAGTTCATTAATGCTAAAGCAATTT
CATTTAACGATGTAGCTGTCCAAGATGAAAATATGTTGTTATCAGAAATTAAAACTATTAAAAATAATAAAATAATAGTT
AAAAAAGGTAAGAAAAAATACTATTTATTGGAAATTAAATAA

Upstream 100 bases:

>100_bases
ACAGGAGATGCAGTTTTAATAAACGGAAAAGAACAGGCTAAGTTTGGTAGAAAAAACATAGCTTCTGATCGTCTAACACA
AATAAAATAAAGGAGAATTT

Downstream 100 bases:

>100_bases
AAAATTCCAAAAAAACATTTAACATTTGGAAAAAAATATATATAATAAAAATAGGGGATAAGAATTTATCCCCCCGCATA
AAAATTGCTAAAGCAGTTTT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 413; Mature: 413

Protein sequence:

>413_residues
MNIIKELEWRGLVKQITNEERLLKAQNDGAAVYCGFDPTADSLHVGHLMMIVTLKRFDKAGFQAIGLIGGGTGMIGDPSF
KADERKLQTDEQVKFHATAIQNQLLRIIPDVTFANNVDWLGNMSLIDFLRDVGKDFNISYLLNKDSIATRISTGLSVTEF
SYTMLQAYDFYNLYINHNCKVQIGGSDQWGNITSGTDYISTRVGSANTEAAGFTIPLLTKSDGQKFGKTESGAVWLDSNK
TSVYDFYQFWINQDDNDCVKMLKYLTFLTKEEIDELEAKHKEAPHLRTMQKTLASEITKFVHGDKELNKAIKLTDAFFTG
NILNLDDDLLELAIKSIPTIQLEKTTSAIDAIVNVNAASSKREAREFINAKAISFNDVAVQDENMLLSEIKTIKNNKIIV
KKGKKKYYLLEIK

Sequences:

>Translated_413_residues
MNIIKELE*RGLVKQITNEERLLKAQNDGAAVYCGFDPTADSLHVGHLMMIVTLKRFDKAGFQAIGLIGGGTGMIGDPSF
KADERKLQTDEQVKFHATAIQNQLLRIIPDVTFANNVD*LGNMSLIDFLRDVGKDFNISYLLNKDSIATRISTGLSVTEF
SYTMLQAYDFYNLYINHNCKVQIGGSDQ*GNITSGTDYISTRVGSANTEAAGFTIPLLTKSDGQKFGKTESGAV*LDSNK
TSVYDFYQF*INQDDNDCVKMLKYLTFLTKEEIDELEAKHKEAPHLRTMQKTLASEITKFVHGDKELNKAIKLTDAFFTG
NILNLDDDLLELAIKSIPTIQLEKTTSAIDAIVNVNAASSKREAREFINAKAISFNDVAVQDENMLLSEIKTIKNNKIIV
KKGKKKYYLLEIK
>Mature_413_residues
MNIIKELE*RGLVKQITNEERLLKAQNDGAAVYCGFDPTADSLHVGHLMMIVTLKRFDKAGFQAIGLIGGGTGMIGDPSF
KADERKLQTDEQVKFHATAIQNQLLRIIPDVTFANNVD*LGNMSLIDFLRDVGKDFNISYLLNKDSIATRISTGLSVTEF
SYTMLQAYDFYNLYINHNCKVQIGGSDQ*GNITSGTDYISTRVGSANTEAAGFTIPLLTKSDGQKFGKTESGAV*LDSNK
TSVYDFYQF*INQDDNDCVKMLKYLTFLTKEEIDELEAKHKEAPHLRTMQKTLASEITKFVHGDKELNKAIKLTDAFFTG
NILNLDDDLLELAIKSIPTIQLEKTTSAIDAIVNVNAASSKREAREFINAKAISFNDVAVQDENMLLSEIKTIKNNKIIV
KKGKKKYYLLEIK

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=402, Percent_Identity=36.5671641791045, Blast_Score=226, Evalue=3e-59,
Organism=Escherichia coli, GI1787925, Length=426, Percent_Identity=42.9577464788732, Blast_Score=324, Evalue=7e-90,
Organism=Caenorhabditis elegans, GI25145802, Length=295, Percent_Identity=38.3050847457627, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI25145805, Length=204, Percent_Identity=39.2156862745098, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6325160, Length=449, Percent_Identity=33.4075723830735, Blast_Score=209, Evalue=8e-55,
Organism=Drosophila melanogaster, GI20130383, Length=408, Percent_Identity=33.578431372549, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45354; Mature: 45354

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS50889 S4 ; PS00318 HMG_COA_REDUCTASE_2 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIIKELERGLVKQITNEERLLKAQNDGAAVYCGFDPTADSLHVGHLMMIVTLKRFDKAG
CCHHHHHHHHHHHHHCCHHHHEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
FQAIGLIGGGTGMIGDPSFKADERKLQTDEQVKFHATAIQNQLLRIIPDVTFANNVDLGN
CEEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
MSLIDFLRDVGKDFNISYLLNKDSIATRISTGLSVTEFSYTMLQAYDFYNLYINHNCKVQ
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEHHHHHHHHHHEEEEEEEECCCEEEE
IGGSDQGNITSGTDYISTRVGSANTEAAGFTIPLLTKSDGQKFGKTESGAVLDSNKTSVY
ECCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCHHH
DFYQFINQDDNDCVKMLKYLTFLTKEEIDELEAKHKEAPHLRTMQKTLASEITKFVHGDK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
ELNKAIKLTDAFFTGNILNLDDDLLELAIKSIPTIQLEKTTSAIDAIVNVNAASSKREAR
HHHHHHHHHHHHEECCEECCCHHHHHHHHHHCCCEEEECHHHHHHHHEECCCCCHHHHHH
EFINAKAISFNDVAVQDENMLLSEIKTIKNNKIIVKKGKKKYYLLEIK
HHHHHCEEECCCEEEECCHHHHHHHHHHCCCEEEEEECCCEEEEEEEC
>Mature Secondary Structure
MNIIKELERGLVKQITNEERLLKAQNDGAAVYCGFDPTADSLHVGHLMMIVTLKRFDKAG
CCHHHHHHHHHHHHHCCHHHHEEECCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCC
FQAIGLIGGGTGMIGDPSFKADERKLQTDEQVKFHATAIQNQLLRIIPDVTFANNVDLGN
CEEEEEEECCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCCEECCCCCCCC
MSLIDFLRDVGKDFNISYLLNKDSIATRISTGLSVTEFSYTMLQAYDFYNLYINHNCKVQ
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHCCCEEHHHHHHHHHHEEEEEEEECCCEEEE
IGGSDQGNITSGTDYISTRVGSANTEAAGFTIPLLTKSDGQKFGKTESGAVLDSNKTSVY
ECCCCCCCCCCCCHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEECCCCHHH
DFYQFINQDDNDCVKMLKYLTFLTKEEIDELEAKHKEAPHLRTMQKTLASEITKFVHGDK
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCH
ELNKAIKLTDAFFTGNILNLDDDLLELAIKSIPTIQLEKTTSAIDAIVNVNAASSKREAR
HHHHHHHHHHHHEECCEECCCHHHHHHHHHHCCCEEEECHHHHHHHHEECCCCCHHHHHH
EFINAKAISFNDVAVQDENMLLSEIKTIKNNKIIVKKGKKKYYLLEIK
HHHHHCEEECCCEEEECCHHHHHHHHHHCCCEEEEEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA