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The map label for this gene is tsf [H]

Identifier: 50365377

GI number: 50365377

Start: 651147

End: 652040

Strand: Reverse

Name: tsf [H]

Synonym: Mfl560

Alternate gene names: 50365377

Gene position: 652040-651147 (Counterclockwise)

Preceding gene: 50365378

Following gene: 50365376

Centisome position: 82.2

GC content: 31.32

Gene sequence:

>894_bases
ATGGCTGTAAACGCACAATTAATTAAAGAATTAAGAGAAATTACACAAGCTGGTATGATGGACTGTAAAAAAGCTTTAGA
AGCAACTGATGGAAACATCGAAGATGCAATCGTTTGATTAAGAGAAAACGGATTAGCTAAAGCTGCTAAAAAATCAGACC
GTGTAGCTGCTGAGGGTGTAGCATTAGCTAAAGAAGACGACAAAAAAGTTGTTATTCTTGAAGTTAACTCAGAAACTGAT
TTCGTTGCACAAAACGAAAAATTTATAAATTTAATCGATGAAATTGCTAATGTATTATTAGCTTCAAATGCTAAAACTTT
AGAAGAAGGTTTAGCATTAAAAACAAACTCTGGAGAAACAATTGAACAAGTTTTAGTTAGTGCTACAGCAACAATTGGAG
AAAAAATTCAATTAAGAAGATTTACTTTAATTGAAAAAGAAGCTGGAAATACAACTACTTTATACAATCATGCAAACAAA
AGAGTTTCAGTTGCATTGAACTTTAAAGGAACACTTGATTCAACAGATGCATACAACTTAGCTATGCACGTTGCCGCAAT
GAGTCCACAATACAAAAGCATTGATGAAATCCCTGCTGAGTTTAAAGAATCAGAATTTAGCATCATTAAAGCTGAAGCTA
AAGAAGATCCAAAATTACAAGGTAAACCAGAAAATGTTTTAGAAAACATTTTAAAAGGAAAATTATCAAAACGTTTATCA
GAAATTAGTTTAGTAGACCAACAATATGTTGTTGATGAAAGTTTTAAAGTAGGTCAATTCTTAGAATCTAAAAAAGTAAC
TTTAATTGACATGGTTAGATATGAAGTTGGAGAAGGAATTGAAAAAGTAGTTACTGACTTTGCAAGTGAAGTAGCTGCTC
AATTAGGTAACTAG

Upstream 100 bases:

>100_bases
ACGTGAAAGAACAGAAGGCGAAAACAACTACTCAAACAACAGAAGCTGAAATAAACCTGAAAGAACAAATAACTAATAAA
ATCAAAAGGAGAAAACACAT

Downstream 100 bases:

>100_bases
TTATGAGTTCAAAATTTTTAATTATGAGTATCGTGTTAATTGGAGTTTCATTACTTTTATTATTAACACTAGCATTTTAT
TACTACCAATTTATAAAACT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MAVNAQLIKELREITQAGMMDCKKALEATDGNIEDAIVWLRENGLAKAAKKSDRVAAEGVALAKEDDKKVVILEVNSETD
FVAQNEKFINLIDEIANVLLASNAKTLEEGLALKTNSGETIEQVLVSATATIGEKIQLRRFTLIEKEAGNTTTLYNHANK
RVSVALNFKGTLDSTDAYNLAMHVAAMSPQYKSIDEIPAEFKESEFSIIKAEAKEDPKLQGKPENVLENILKGKLSKRLS
EISLVDQQYVVDESFKVGQFLESKKVTLIDMVRYEVGEGIEKVVTDFASEVAAQLGN

Sequences:

>Translated_297_residues
MAVNAQLIKELREITQAGMMDCKKALEATDGNIEDAIV*LRENGLAKAAKKSDRVAAEGVALAKEDDKKVVILEVNSETD
FVAQNEKFINLIDEIANVLLASNAKTLEEGLALKTNSGETIEQVLVSATATIGEKIQLRRFTLIEKEAGNTTTLYNHANK
RVSVALNFKGTLDSTDAYNLAMHVAAMSPQYKSIDEIPAEFKESEFSIIKAEAKEDPKLQGKPENVLENILKGKLSKRLS
EISLVDQQYVVDESFKVGQFLESKKVTLIDMVRYEVGEGIEKVVTDFASEVAAQLGN
>Mature_296_residues
AVNAQLIKELREITQAGMMDCKKALEATDGNIEDAIV*LRENGLAKAAKKSDRVAAEGVALAKEDDKKVVILEVNSETDF
VAQNEKFINLIDEIANVLLASNAKTLEEGLALKTNSGETIEQVLVSATATIGEKIQLRRFTLIEKEAGNTTTLYNHANKR
VSVALNFKGTLDSTDAYNLAMHVAAMSPQYKSIDEIPAEFKESEFSIIKAEAKEDPKLQGKPENVLENILKGKLSKRLSE
ISLVDQQYVVDESFKVGQFLESKKVTLIDMVRYEVGEGIEKVVTDFASEVAAQLGN

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=309, Percent_Identity=23.3009708737864, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1786366, Length=292, Percent_Identity=40.4109589041096, Blast_Score=181, Evalue=4e-47,
Organism=Caenorhabditis elegans, GI17561440, Length=306, Percent_Identity=27.1241830065359, Blast_Score=81, Evalue=6e-16,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 32434; Mature: 32303

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVNAQLIKELREITQAGMMDCKKALEATDGNIEDAIVLRENGLAKAAKKSDRVAAEGVA
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCHHHCCEE
LAKEDDKKVVILEVNSETDFVAQNEKFINLIDEIANVLLASNAKTLEEGLALKTNSGETI
EEECCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCEEECCCCHHH
EQVLVSATATIGEKIQLRRFTLIEKEAGNTTTLYNHANKRVSVALNFKGTLDSTDAYNLA
HHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCEEEEEEEECCCCCCCCHHHHH
MHVAAMSPQYKSIDEIPAEFKESEFSIIKAEAKEDPKLQGKPENVLENILKGKLSKRLSE
HHHHHCCCCCCCHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ISLVDQQYVVDESFKVGQFLESKKVTLIDMVRYEVGEGIEKVVTDFASEVAAQLGN
HHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AVNAQLIKELREITQAGMMDCKKALEATDGNIEDAIVLRENGLAKAAKKSDRVAAEGVA
CCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHCCHHHCCEE
LAKEDDKKVVILEVNSETDFVAQNEKFINLIDEIANVLLASNAKTLEEGLALKTNSGETI
EEECCCCEEEEEEECCCCCEEECCHHHHHHHHHHHHHHHHCCCHHHHHCCEEECCCCHHH
EQVLVSATATIGEKIQLRRFTLIEKEAGNTTTLYNHANKRVSVALNFKGTLDSTDAYNLA
HHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEECCCCEEEEEEEECCCCCCCCHHHHH
MHVAAMSPQYKSIDEIPAEFKESEFSIIKAEAKEDPKLQGKPENVLENILKGKLSKRLSE
HHHHHCCCCCCCHHHHHHHHCCCCHHEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
ISLVDQQYVVDESFKVGQFLESKKVTLIDMVRYEVGEGIEKVVTDFASEVAAQLGN
HHHHHHHHHHCCCCHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA