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The map label for this gene is rnhB [H]

Identifier: 50365354

GI number: 50365354

Start: 633730

End: 634356

Strand: Reverse

Name: rnhB [H]

Synonym: Mfl537

Alternate gene names: 50365354

Gene position: 634356-633730 (Counterclockwise)

Preceding gene: 50365355

Following gene: 50365353

Centisome position: 79.97

GC content: 28.23

Gene sequence:

>627_bases
ATGATAGATGAATCAAGAATATTATTTGATAGTCAAATTAGAGAAAAATATAAAACTGATTTTATTAGTGGCAGTGATGA
AGCTGGTAGAGGTGCAATGGCAGGACCTGTTGTTGTAGCAAGTGTAATATTGCCAAAAGATTATAAAAATTGTTTAATTA
GAGATTCAAAAAAATTAAGCAAAAAGCAAAGAGAATCACTTTGCGAAGAAATAAAGTCAGTAGCTATAAGCTATGCTATT
GAAATAATTGAAGCAGATGAAGTAGATATATTAAATCCTAAACAAGCTAGCAGAATTGGTATGACAAAATCTATTATGAA
TTTAAAACCTAAGCCTGATTTATGTTTGATTGATGCAGAAAATATTATTATTCAAGAATATGTATGTGCCCCATTTATTA
AAGGGGATGATTTAAGTCAATCAATTGCAGCAGCTTCAATTTTAGCAAAAACTACAAGAGACAATATAATGTTGGATTAT
GCTAAAATTTATCCTGAATATAATTTTGAATCTCATAAAGGTTATTGTGTAAAAGAACATGTTGAAAAAACTAAAATGCT
TGGTGTTTTGCCAATACACCGAAAAACCTACAAACCAATTAAAGATATTCTAGAAAATAACTTTTAA

Upstream 100 bases:

>100_bases
ATAAAGTTTCTTTTGAAAGAATTTTAGAAGTTGTTCCAGAGGAAATGGAAAAAGCATCAAAAATTAAAAATGAAAAAGCA
ATCGAGGATATTTCAGATTT

Downstream 100 bases:

>100_bases
TGTTATTTTTTTTATTTTTTTCTTAATTATATTATGTCAGGTATCTGACACATATAAAAAAAGGAGTTCATATGAATTTA
GTAAATATAATTGGTCAAAT

Product: ribonuclease HII, primer excision

Products: NA

Alternate protein names: RNase HII [H]

Number of amino acids: Translated: 208; Mature: 208

Protein sequence:

>208_residues
MIDESRILFDSQIREKYKTDFISGSDEAGRGAMAGPVVVASVILPKDYKNCLIRDSKKLSKKQRESLCEEIKSVAISYAI
EIIEADEVDILNPKQASRIGMTKSIMNLKPKPDLCLIDAENIIIQEYVCAPFIKGDDLSQSIAAASILAKTTRDNIMLDY
AKIYPEYNFESHKGYCVKEHVEKTKMLGVLPIHRKTYKPIKDILENNF

Sequences:

>Translated_208_residues
MIDESRILFDSQIREKYKTDFISGSDEAGRGAMAGPVVVASVILPKDYKNCLIRDSKKLSKKQRESLCEEIKSVAISYAI
EIIEADEVDILNPKQASRIGMTKSIMNLKPKPDLCLIDAENIIIQEYVCAPFIKGDDLSQSIAAASILAKTTRDNIMLDY
AKIYPEYNFESHKGYCVKEHVEKTKMLGVLPIHRKTYKPIKDILENNF
>Mature_208_residues
MIDESRILFDSQIREKYKTDFISGSDEAGRGAMAGPVVVASVILPKDYKNCLIRDSKKLSKKQRESLCEEIKSVAISYAI
EIIEADEVDILNPKQASRIGMTKSIMNLKPKPDLCLIDAENIIIQEYVCAPFIKGDDLSQSIAAASILAKTTRDNIMLDY
AKIYPEYNFESHKGYCVKEHVEKTKMLGVLPIHRKTYKPIKDILENNF

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family [H]

Homologues:

Organism=Escherichia coli, GI1786380, Length=186, Percent_Identity=38.7096774193548, Blast_Score=129, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01351 RNase_HII [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 23508; Mature: 23508

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDESRILFDSQIREKYKTDFISGSDEAGRGAMAGPVVVASVILPKDYKNCLIRDSKKLS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHH
KKQRESLCEEIKSVAISYAIEIIEADEVDILNPKQASRIGMTKSIMNLKPKPDLCLIDAE
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECC
NIIIQEYVCAPFIKGDDLSQSIAAASILAKTTRDNIMLDYAKIYPEYNFESHKGYCVKEH
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCHHHHH
VEKTKMLGVLPIHRKTYKPIKDILENNF
HHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDESRILFDSQIREKYKTDFISGSDEAGRGAMAGPVVVASVILPKDYKNCLIRDSKKLS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCHHHHH
KKQRESLCEEIKSVAISYAIEIIEADEVDILNPKQASRIGMTKSIMNLKPKPDLCLIDAE
HHHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECC
NIIIQEYVCAPFIKGDDLSQSIAAASILAKTTRDNIMLDYAKIYPEYNFESHKGYCVKEH
CHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHCCCCCCCCCCCCHHHHH
VEKTKMLGVLPIHRKTYKPIKDILENNF
HHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA