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The map label for this gene is trmFO2 [H]

Identifier: 50365349

GI number: 50365349

Start: 627269

End: 628609

Strand: Reverse

Name: trmFO2 [H]

Synonym: Mfl532

Alternate gene names: 50365349

Gene position: 628609-627269 (Counterclockwise)

Preceding gene: 50365350

Following gene: 50365348

Centisome position: 79.25

GC content: 28.86

Gene sequence:

>1341_bases
ATGAAAAATAAAACAGTAAATATTATTGGAGCTGGTTTAGCAGGAACAGAAGCTGCATACCAACTAGCTAAAAGAAATAT
AAAAGTTAAACTTTTCGAAGTTAAAAGAATAACTAAAAATCCCGTCCAAGTCTTGGACGGGTTTGCTGAATTAGTTTGCT
CTAATTCTCTAAGAAGTAACGAATTAACTAATGCAGTCGGTACATTAAAAGAAGAAATGAGACTTTTAGATTCATTAATT
ATAAGAGCGGCTGAATATGCACAAGTTCCAGCTGGAGGAAGTTTAGCCGTTGATAGAGTTTTATTTTCTGAATATATAAC
AAAAGAAATAAGTTCTAATGAAAACATTGAAGTCATAGATCTTGAAGTTGAAAATATAAACCCAAATGAATTAACACTTA
TTGCATCAGGGCCTTTAACAACAAATAAACTACAAGAGCAAATTACAAAACTTATAGGAAATGATGATTTTTACTTTTAT
GATGCAGTTGCTCCGATTATAGAAAAAGAATCTATAAATATGGAAATTGCATATCAAAAAAATAGATATGACAAAGGTGA
AACAAGCGATTATATAAATTGTCCAATGTCAAAAGAAGAATATTTAAATTTTTATAAAGAACTAATTAATGCAGAAATTT
CTATAGGGCATTTACCTGGTGAAGCAGAATTAAAGTACTTTGAAGGGTGTATGCCAGTAGAAGCTATGGCCAAAAGAGGT
GTTGATACTTTATTGTTTGGTCCAATGAAACCAAGAGGTTTAAATAAACCTGATGGAACAAGAAATCATGCTGTTGTTCA
ACTACGCCAAGATGATGCAAATGATAGACTTTATAATATTGTTGGTTTCCAAACAAACTTAAAATGACCTGAACAAAAAA
GAATTTTTTCAATGATACCAGGTTTAGAAAACGCTAAGTTTGTTAGATATGGTGTAATGCATAAAAATAATTTTATAAAC
TCGCCTAAAGTTCTTAACAAATATTTACAATTAAAAACTAATGAAAACATATTTTTTGCTGGTCAAATAACAGGTGTAGA
AGGATATGTTGAATCTTGTGTTTCTGGTTTGATCACAGCTATAAATATTGCTAATTTCATTAATGGAAAAGAAATGCAAG
TACCATCATCTAAATCAGTTTCAGGTGCTTTACTTAACTATATAAACAAAGCTTCTATAACAAACTTTCAACCAATGAAA
GCAAATTGAGGAATAGTTGATGAAATAGAATTTAAAAGACCTAATAGAAAAGTAACTAAGCAAGAAATTAAAAAAATGAA
AAATGAAAAGATTTCAGAATTAGCAATTGATTCTATTAAGGATTTTATTAAAAATATTTAG

Upstream 100 bases:

>100_bases
GTGCAAACGGAAAAGGTATTGTGCAACAAGGTAGTTATGAAGAATTAATTTCAACACCTGGAAGATTTAAAAAATTATAT
GAAGCAGGATTGATGAATTA

Downstream 100 bases:

>100_bases
AAGGAGCAAATATTATGATAAATCGTTTAGACACTGTTTTTTGAGCATATTTTGATGAATATATTAAAAAAGACTCATCA
TTGATTTTTAAAAAAATTAA

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase 2; Folate-dependent tRNA(M-5-U54)-methyltransferase 2 [H]

Number of amino acids: Translated: 446; Mature: 446

Protein sequence:

>446_residues
MKNKTVNIIGAGLAGTEAAYQLAKRNIKVKLFEVKRITKNPVQVLDGFAELVCSNSLRSNELTNAVGTLKEEMRLLDSLI
IRAAEYAQVPAGGSLAVDRVLFSEYITKEISSNENIEVIDLEVENINPNELTLIASGPLTTNKLQEQITKLIGNDDFYFY
DAVAPIIEKESINMEIAYQKNRYDKGETSDYINCPMSKEEYLNFYKELINAEISIGHLPGEAELKYFEGCMPVEAMAKRG
VDTLLFGPMKPRGLNKPDGTRNHAVVQLRQDDANDRLYNIVGFQTNLKWPEQKRIFSMIPGLENAKFVRYGVMHKNNFIN
SPKVLNKYLQLKTNENIFFAGQITGVEGYVESCVSGLITAINIANFINGKEMQVPSSKSVSGALLNYINKASITNFQPMK
ANWGIVDEIEFKRPNRKVTKQEIKKMKNEKISELAIDSIKDFIKNI

Sequences:

>Translated_446_residues
MKNKTVNIIGAGLAGTEAAYQLAKRNIKVKLFEVKRITKNPVQVLDGFAELVCSNSLRSNELTNAVGTLKEEMRLLDSLI
IRAAEYAQVPAGGSLAVDRVLFSEYITKEISSNENIEVIDLEVENINPNELTLIASGPLTTNKLQEQITKLIGNDDFYFY
DAVAPIIEKESINMEIAYQKNRYDKGETSDYINCPMSKEEYLNFYKELINAEISIGHLPGEAELKYFEGCMPVEAMAKRG
VDTLLFGPMKPRGLNKPDGTRNHAVVQLRQDDANDRLYNIVGFQTNLK*PEQKRIFSMIPGLENAKFVRYGVMHKNNFIN
SPKVLNKYLQLKTNENIFFAGQITGVEGYVESCVSGLITAINIANFINGKEMQVPSSKSVSGALLNYINKASITNFQPMK
AN*GIVDEIEFKRPNRKVTKQEIKKMKNEKISELAIDSIKDFIKNI
>Mature_446_residues
MKNKTVNIIGAGLAGTEAAYQLAKRNIKVKLFEVKRITKNPVQVLDGFAELVCSNSLRSNELTNAVGTLKEEMRLLDSLI
IRAAEYAQVPAGGSLAVDRVLFSEYITKEISSNENIEVIDLEVENINPNELTLIASGPLTTNKLQEQITKLIGNDDFYFY
DAVAPIIEKESINMEIAYQKNRYDKGETSDYINCPMSKEEYLNFYKELINAEISIGHLPGEAELKYFEGCMPVEAMAKRG
VDTLLFGPMKPRGLNKPDGTRNHAVVQLRQDDANDRLYNIVGFQTNLK*PEQKRIFSMIPGLENAKFVRYGVMHKNNFIN
SPKVLNKYLQLKTNENIFFAGQITGVEGYVESCVSGLITAINIANFINGKEMQVPSSKSVSGALLNYINKASITNFQPMK
AN*GIVDEIEFKRPNRKVTKQEIKKMKNEKISELAIDSIKDFIKNI

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs [H]

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004417
- InterPro:   IPR002218 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: =2.1.1.74 [H]

Molecular weight: Translated: 49780; Mature: 49780

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNKTVNIIGAGLAGTEAAYQLAKRNIKVKLFEVKRITKNPVQVLDGFAELVCSNSLRSN
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEHHHCCCHHHHHHHHHHHHHCCCCCCH
ELTNAVGTLKEEMRLLDSLIIRAAEYAQVPAGGSLAVDRVLFSEYITKEISSNENIEVID
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
LEVENINPNELTLIASGPLTTNKLQEQITKLIGNDDFYFYDAVAPIIEKESINMEIAYQK
EEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEEC
NRYDKGETSDYINCPMSKEEYLNFYKELINAEISIGHLPGEAELKYFEGCMPVEAMAKRG
CCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCHHHHHHCC
VDTLLFGPMKPRGLNKPDGTRNHAVVQLRQDDANDRLYNIVGFQTNLKPEQKRIFSMIPG
CCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
LENAKFVRYGVMHKNNFINSPKVLNKYLQLKTNENIFFAGQITGVEGYVESCVSGLITAI
CCCCCEEEEEEEECCCCCCCHHHHHHHHHEECCCCEEEEEEECCHHHHHHHHHHHHHHHH
NIANFINGKEMQVPSSKSVSGALLNYINKASITNFQPMKANGIVDEIEFKRPNRKVTKQE
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHH
IKKMKNEKISELAIDSIKDFIKNI
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKNKTVNIIGAGLAGTEAAYQLAKRNIKVKLFEVKRITKNPVQVLDGFAELVCSNSLRSN
CCCCEEEEEECCCCCHHHHHHHHHCCCEEEEEEEHHHCCCHHHHHHHHHHHHHCCCCCCH
ELTNAVGTLKEEMRLLDSLIIRAAEYAQVPAGGSLAVDRVLFSEYITKEISSNENIEVID
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEE
LEVENINPNELTLIASGPLTTNKLQEQITKLIGNDDFYFYDAVAPIIEKESINMEIAYQK
EEECCCCCCEEEEEECCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHCCCCEEEEEEC
NRYDKGETSDYINCPMSKEEYLNFYKELINAEISIGHLPGEAELKYFEGCMPVEAMAKRG
CCCCCCCCCCCEECCCCHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCHHHHHHCC
VDTLLFGPMKPRGLNKPDGTRNHAVVQLRQDDANDRLYNIVGFQTNLKPEQKRIFSMIPG
CCEEEECCCCCCCCCCCCCCCCCEEEEEECCCCCCCEEEEEECCCCCCHHHHHHHHHCCC
LENAKFVRYGVMHKNNFINSPKVLNKYLQLKTNENIFFAGQITGVEGYVESCVSGLITAI
CCCCCEEEEEEEECCCCCCCHHHHHHHHHEECCCCEEEEEEECCHHHHHHHHHHHHHHHH
NIANFINGKEMQVPSSKSVSGALLNYINKASITNFQPMKANGIVDEIEFKRPNRKVTKQE
HHHHHCCCCEEECCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCCCCHHHHH
IKKMKNEKISELAIDSIKDFIKNI
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA