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The map label for this gene is nrdF [H]

Identifier: 50365347

GI number: 50365347

Start: 624396

End: 625418

Strand: Reverse

Name: nrdF [H]

Synonym: Mfl530

Alternate gene names: 50365347

Gene position: 625418-624396 (Counterclockwise)

Preceding gene: 50365348

Following gene: 50365346

Centisome position: 78.85

GC content: 30.69

Gene sequence:

>1023_bases
ATGGCAAAAATAAAAAACCAATATTACAACGAGTCTGTTTCGCCAATTGAATATGCGCAACAAGGATTTAAAGGAAAAAT
GCGTTCAGTAAACTGAAACGTAGTAAATGATGAAAAAGATTTAGAGGTATGAAATAGAATTACACAAAACTTCTGATTGC
CTGAAAAAATTCCAGTTTCAAATGATTTAACTTCATGAAGAACTTTGACACCAGAATGACAAGAATTAATTACAAGAACT
TTTACAGGATTAACATTGTTAGATACAATTCAAGCTACTGTTGGTGATGTGGCTCAAGTTCCTAACTCATTAACTGACCA
TGAACAAGTAATTTACACAAACTTTGCATTTATGGTTGCAGTTCACGCTAGATCATATGGTTCAATCTTTTCAACTTTAT
GTTCAAGTGAACAAATTGAAGAGGCTCATGAATGAGTTATCAATACAGAAACATTACAAGAAAGAGCTAAAGCATTAATT
CCTTATTATGTGAATGATGACCCTTTAAAGTCAAAAGTTGCAGCTGCTTTAATGCCAGGCTTCTTATTATATGGAGGCTT
CTATTTACCATTTTACCTATCAGCTAGAGGTAAATTACCAAACACTTCAGATATTATTAGATTAATATTAAGAGATAAAG
TTATACATAACTACTATAGTGGTTATAAATATCAAAAGAAAGTTGCTAAACTTTCTCCAGAAAAACAAGCTGAAATGAAA
GAATTTGTTTTTAAATTATTATATGAATTAATAGATTTAGAAAAAGCTTATTTGAAAGAATTGTATGAGGATTTTGGATT
AGCTGATGATGCTATTAGATTTAGTGTTTACAACGCAGGTAAATTTTTACAAAATTTAGGTTATGATTCACCGTTTACAG
AAGAAGAAACAAGAATTGAGCCAGAAATATTCACACAATTATCAGCTAGAGCTGATGAAAACCATGATTTCTTTTCAGGG
AATGGCTCATCATATATTATGGGAGTTTCAGAAGAAACTGAAGATGACGATTGGGAGTTTTAA

Upstream 100 bases:

>100_bases
CCACTCTTTAATTATTAAAGTTTCTTTTTATGTCCAAAAGACAAGAAGAAACTTTTTTATTTAGTTGAATTTATAATAAG
AGAAAAAGAAAGGATATTAT

Downstream 100 bases:

>100_bases
TATATGCACGATGATATTAAGTTAGTTTCAGGTGAAGAAATTGTTAAGCCTACAGGAGAAGTGCATGTAGTTTACTTCTC
ATCAATTTCAAATAACACAC

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MAKIKNQYYNESVSPIEYAQQGFKGKMRSVNWNVVNDEKDLEVWNRITQNFWLPEKIPVSNDLTSWRTLTPEWQELITRT
FTGLTLLDTIQATVGDVAQVPNSLTDHEQVIYTNFAFMVAVHARSYGSIFSTLCSSEQIEEAHEWVINTETLQERAKALI
PYYVNDDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLSPEKQAEMK
EFVFKLLYELIDLEKAYLKELYEDFGLADDAIRFSVYNAGKFLQNLGYDSPFTEEETRIEPEIFTQLSARADENHDFFSG
NGSSYIMGVSEETEDDDWEF

Sequences:

>Translated_340_residues
MAKIKNQYYNESVSPIEYAQQGFKGKMRSVN*NVVNDEKDLEV*NRITQNF*LPEKIPVSNDLTS*RTLTPE*QELITRT
FTGLTLLDTIQATVGDVAQVPNSLTDHEQVIYTNFAFMVAVHARSYGSIFSTLCSSEQIEEAHE*VINTETLQERAKALI
PYYVNDDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLSPEKQAEMK
EFVFKLLYELIDLEKAYLKELYEDFGLADDAIRFSVYNAGKFLQNLGYDSPFTEEETRIEPEIFTQLSARADENHDFFSG
NGSSYIMGVSEETEDDDWEF
>Mature_339_residues
AKIKNQYYNESVSPIEYAQQGFKGKMRSVN*NVVNDEKDLEV*NRITQNF*LPEKIPVSNDLTS*RTLTPE*QELITRTF
TGLTLLDTIQATVGDVAQVPNSLTDHEQVIYTNFAFMVAVHARSYGSIFSTLCSSEQIEEAHE*VINTETLQERAKALIP
YYVNDDPLKSKVAAALMPGFLLYGGFYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLSPEKQAEMKE
FVFKLLYELIDLEKAYLKELYEDFGLADDAIRFSVYNAGKFLQNLGYDSPFTEEETRIEPEIFTQLSARADENHDFFSGN
GSSYIMGVSEETEDDDWEF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Escherichia coli, GI1789031, Length=316, Percent_Identity=51.2658227848101, Blast_Score=319, Evalue=2e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 38081; Mature: 37950

Theoretical pI: Translated: 4.50; Mature: 4.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKIKNQYYNESVSPIEYAQQGFKGKMRSVNNVVNDEKDLEVNRITQNFLPEKIPVSNDL
CCCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
TSRTLTPEQELITRTFTGLTLLDTIQATVGDVAQVPNSLTDHEQVIYTNFAFMVAVHARS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
YGSIFSTLCSSEQIEEAHEVINTETLQERAKALIPYYVNDDPLKSKVAAALMPGFLLYGG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHH
FYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLSPEKQAEMKEFVFKL
HHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHH
LYELIDLEKAYLKELYEDFGLADDAIRFSVYNAGKFLQNLGYDSPFTEEETRIEPEIFTQ
HHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
LSARADENHDFFSGNGSSYIMGVSEETEDDDWEF
HHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
AKIKNQYYNESVSPIEYAQQGFKGKMRSVNNVVNDEKDLEVNRITQNFLPEKIPVSNDL
CCCCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCC
TSRTLTPEQELITRTFTGLTLLDTIQATVGDVAQVPNSLTDHEQVIYTNFAFMVAVHARS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
YGSIFSTLCSSEQIEEAHEVINTETLQERAKALIPYYVNDDPLKSKVAAALMPGFLLYGG
HHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHH
FYLPFYLSARGKLPNTSDIIRLILRDKVIHNYYSGYKYQKKVAKLSPEKQAEMKEFVFKL
HHHHHHEECCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCHHHHHHHHHHHHH
LYELIDLEKAYLKELYEDFGLADDAIRFSVYNAGKFLQNLGYDSPFTEEETRIEPEIFTQ
HHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHCCCCCCCCCHHHCCCHHHHHH
LSARADENHDFFSGNGSSYIMGVSEETEDDDWEF
HHHCCCCCCCCCCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]