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The map label for this gene is ptsI [H]

Identifier: 50365336

GI number: 50365336

Start: 611097

End: 612818

Strand: Reverse

Name: ptsI [H]

Synonym: Mfl519

Alternate gene names: 50365336

Gene position: 612818-611097 (Counterclockwise)

Preceding gene: 50365337

Following gene: 50365335

Centisome position: 77.26

GC content: 30.84

Gene sequence:

>1722_bases
ATGAGTAAACAATTTAAAGGTATTGGAGCTAGTGAAGGTATTGCTGTAGCAAAAGCTTTAGTTCTAGCAGAAGACCATAT
AGAAATTAAAAAAACAAAAGTTTCTGATATTGAAGCAGAAGTTTCAAAATTAGAAAATGCAGTTAATAAATCAATCGAAG
ATTTAGAGAATTTAAAAGCTACTACACTTGAAAAATTAGGTCCAGAAAAAGCAGCTATTTTTGATGCACATAAAGAAATT
GCATCTGACCCAGCTATTAAAGATGAAATTATAAATGTTATTAAATCTGAATCAATTTGTGCTGAGTTTGCGGCAGAACA
AGTAACTAATAACTTTTATGAAATGTTTGCCTCAATGGATGATCCATATTTTAAAGAAAGAAGTGCAGACATTAAAGATG
TAGCTTCAAGAATTATTAAACACATTTTAGGAATTGCTATTGTTGACCTATCAACAATAAGCGAAGAAGTTATTATTGTT
GCTGAAGATTTAACACCTTCACAAACTGCTCAATTAAATAAACAATTTGTTAAAGGTTTTGCAACTAATATTGGTGGAAG
AACTTCTCATGCTGCTATTATGGCAAGAAGTTTAGAAATTCCTGCAGTTTTAGGTTTAAGAACAATAACAAGTGATGTTA
AAAATGGAGAAGTTTTTGCATTAAACGGAACAACAGGTATTGTTGAATTAGATTTAACAGATACAATTAAATCAGAATAT
GAAAAATTAGCAAAAGAATTTTCTGATTTAAAAGCTGAATTACAAAAATTTAAAGACTTACCTTCAAAAACAGCTGATGG
ACAAGAAAAATTAATTGAAGCAAATATTGGTTCTCCAACTGATGTTGAATCTGTTATTGAACATGGTGGAGAAGGAATTG
GATTATTCCGTTCAGAATTCTTATACATGGACAATGACCACTTCCCAACTGAAGAAGAACAATATATAGCTTACAAACAA
GTTATGGATGCAATGAAAGGTAAATTAGTTGTTATCCGTACATTAGATATCGGTGGAGATAAAAAATTATCATACTTTGA
ATTCCCACATGAAATGAACCCATTCTTAGGATATAGAGCTGTTCGTTTTACATTAGACAGAAAAGATATTTTTAAAGATC
AATTAAGAGCTTTATTAAGAGCTAGTGCTCATGGTGAATTAGGAATTATGTTCCCAATGATTGCAACTGTTGATGAATTC
AAGCGTGCTAAAGCAATCGTAGAAGAATGTAAGGAAGAATTAAGAAAAGAAAATATTGCATTTGACGAAAATGTTCAAGT
TGGAATGATGGTTGAAATTCCTGCCGCTGCTGTTAACGCAGATAAATTTAGTAAATATGCTGATTTCTTCTCAATCGGAA
CAAATGATTTAATTCAATATTCAATGGCTGCTGACCGTATGAGCGAAAATGTTTCATATCTTTACCAACCTTTAAATCCA
GCAATATTAAAATTAATTGATTTAACAATTAAAGGTGCACACAAAAATAATAAATGAGTTGGTATGTGTGGAGAAATGGC
TGGAGATATTCAAGCTTTACCATTACTTTTAGGAATGGGATTAGATGCATTTTCAATGAGTGCAACTTCAATGTTAAGAG
CAAGAGCTTTAATGAGTAAAATTACTATGGCTGAAGCAGAAGAATTAGCTAACAAAGCATTATCTTCAGATGATACTCCT
GAAGTTATTGAATTAGTTGATGCATTCTTAGCAACTAAATAA

Upstream 100 bases:

>100_bases
TAATAATATTTTCCATTTTTTAATATTTTTAATATAAAAAATAGCAATTAAATATTATAATTATATATGTTATGAAATAA
CTTACATAGGAGAAAAAAGT

Downstream 100 bases:

>100_bases
TATTTGAGAATAACGAGATTAAGATCGTTATTTTTATTTTTAAAATAAATAAATAGTATAATAATATAATAAAGAAAGAG
GATAATATGAAAAATTTTAA

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSKQFKGIGASEGIAVAKALVLAEDHIEIKKTKVSDIEAEVSKLENAVNKSIEDLENLKATTLEKLGPEKAAIFDAHKEI
ASDPAIKDEIINVIKSESICAEFAAEQVTNNFYEMFASMDDPYFKERSADIKDVASRIIKHILGIAIVDLSTISEEVIIV
AEDLTPSQTAQLNKQFVKGFATNIGGRTSHAAIMARSLEIPAVLGLRTITSDVKNGEVFALNGTTGIVELDLTDTIKSEY
EKLAKEFSDLKAELQKFKDLPSKTADGQEKLIEANIGSPTDVESVIEHGGEGIGLFRSEFLYMDNDHFPTEEEQYIAYKQ
VMDAMKGKLVVIRTLDIGGDKKLSYFEFPHEMNPFLGYRAVRFTLDRKDIFKDQLRALLRASAHGELGIMFPMIATVDEF
KRAKAIVEECKEELRKENIAFDENVQVGMMVEIPAAAVNADKFSKYADFFSIGTNDLIQYSMAADRMSENVSYLYQPLNP
AILKLIDLTIKGAHKNNKWVGMCGEMAGDIQALPLLLGMGLDAFSMSATSMLRARALMSKITMAEAEELANKALSSDDTP
EVIELVDAFLATK

Sequences:

>Translated_573_residues
MSKQFKGIGASEGIAVAKALVLAEDHIEIKKTKVSDIEAEVSKLENAVNKSIEDLENLKATTLEKLGPEKAAIFDAHKEI
ASDPAIKDEIINVIKSESICAEFAAEQVTNNFYEMFASMDDPYFKERSADIKDVASRIIKHILGIAIVDLSTISEEVIIV
AEDLTPSQTAQLNKQFVKGFATNIGGRTSHAAIMARSLEIPAVLGLRTITSDVKNGEVFALNGTTGIVELDLTDTIKSEY
EKLAKEFSDLKAELQKFKDLPSKTADGQEKLIEANIGSPTDVESVIEHGGEGIGLFRSEFLYMDNDHFPTEEEQYIAYKQ
VMDAMKGKLVVIRTLDIGGDKKLSYFEFPHEMNPFLGYRAVRFTLDRKDIFKDQLRALLRASAHGELGIMFPMIATVDEF
KRAKAIVEECKEELRKENIAFDENVQVGMMVEIPAAAVNADKFSKYADFFSIGTNDLIQYSMAADRMSENVSYLYQPLNP
AILKLIDLTIKGAHKNNK*VGMCGEMAGDIQALPLLLGMGLDAFSMSATSMLRARALMSKITMAEAEELANKALSSDDTP
EVIELVDAFLATK
>Mature_572_residues
SKQFKGIGASEGIAVAKALVLAEDHIEIKKTKVSDIEAEVSKLENAVNKSIEDLENLKATTLEKLGPEKAAIFDAHKEIA
SDPAIKDEIINVIKSESICAEFAAEQVTNNFYEMFASMDDPYFKERSADIKDVASRIIKHILGIAIVDLSTISEEVIIVA
EDLTPSQTAQLNKQFVKGFATNIGGRTSHAAIMARSLEIPAVLGLRTITSDVKNGEVFALNGTTGIVELDLTDTIKSEYE
KLAKEFSDLKAELQKFKDLPSKTADGQEKLIEANIGSPTDVESVIEHGGEGIGLFRSEFLYMDNDHFPTEEEQYIAYKQV
MDAMKGKLVVIRTLDIGGDKKLSYFEFPHEMNPFLGYRAVRFTLDRKDIFKDQLRALLRASAHGELGIMFPMIATVDEFK
RAKAIVEECKEELRKENIAFDENVQVGMMVEIPAAAVNADKFSKYADFFSIGTNDLIQYSMAADRMSENVSYLYQPLNPA
ILKLIDLTIKGAHKNNK*VGMCGEMAGDIQALPLLLGMGLDAFSMSATSMLRARALMSKITMAEAEELANKALSSDDTPE
VIELVDAFLATK

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=569, Percent_Identity=47.6274165202109, Blast_Score=524, Evalue=1e-150,
Organism=Escherichia coli, GI48994992, Length=538, Percent_Identity=36.0594795539033, Blast_Score=347, Evalue=2e-96,
Organism=Escherichia coli, GI1789193, Length=577, Percent_Identity=32.2357019064125, Blast_Score=298, Evalue=7e-82,
Organism=Escherichia coli, GI1788726, Length=476, Percent_Identity=35.5042016806723, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI1787994, Length=458, Percent_Identity=30.5676855895196, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI226510935, Length=164, Percent_Identity=29.2682926829268, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63014; Mature: 62883

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQFKGIGASEGIAVAKALVLAEDHIEIKKTKVSDIEAEVSKLENAVNKSIEDLENLKA
CCCCCCCCCCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLEKLGPEKAAIFDAHKEIASDPAIKDEIINVIKSESICAEFAAEQVTNNFYEMFASMD
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPYFKERSADIKDVASRIIKHILGIAIVDLSTISEEVIIVAEDLTPSQTAQLNKQFVKGF
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
ATNIGGRTSHAAIMARSLEIPAVLGLRTITSDVKNGEVFALNGTTGIVELDLTDTIKSEY
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHHH
EKLAKEFSDLKAELQKFKDLPSKTADGQEKLIEANIGSPTDVESVIEHGGEGIGLFRSEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCHHHHHHCCCCCEEEHHHCE
LYMDNDHFPTEEEQYIAYKQVMDAMKGKLVVIRTLDIGGDKKLSYFEFPHEMNPFLGYRA
EEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEECCCCCCCHHCCEE
VRFTLDRKDIFKDQLRALLRASAHGELGIMFPMIATVDEFKRAKAIVEECKEELRKENIA
EEEEECHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDENVQVGMMVEIPAAAVNADKFSKYADFFSIGTNDLIQYSMAADRMSENVSYLYQPLNP
CCCCCCEEEEEECCHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
AILKLIDLTIKGAHKNNKVGMCGEMAGDIQALPLLLGMGLDAFSMSATSMLRARALMSKI
HHHHHHHHHEECCCCCCCCCCCHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TMAEAEELANKALSSDDTPEVIELVDAFLATK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKQFKGIGASEGIAVAKALVLAEDHIEIKKTKVSDIEAEVSKLENAVNKSIEDLENLKA
CCCCCCCCCCCHHHHHHHHHHHHCCHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTLEKLGPEKAAIFDAHKEIASDPAIKDEIINVIKSESICAEFAAEQVTNNFYEMFASMD
HHHHHHCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
DPYFKERSADIKDVASRIIKHILGIAIVDLSTISEEVIIVAEDLTPSQTAQLNKQFVKGF
CCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHH
ATNIGGRTSHAAIMARSLEIPAVLGLRTITSDVKNGEVFALNGTTGIVELDLTDTIKSEY
HHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCCCEEEEEECCCEEEEECCHHHHHHHH
EKLAKEFSDLKAELQKFKDLPSKTADGQEKLIEANIGSPTDVESVIEHGGEGIGLFRSEF
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHEECCCCCCCCHHHHHHCCCCCEEEHHHCE
LYMDNDHFPTEEEQYIAYKQVMDAMKGKLVVIRTLDIGGDKKLSYFEFPHEMNPFLGYRA
EEECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCEECCCCCCCHHCCEE
VRFTLDRKDIFKDQLRALLRASAHGELGIMFPMIATVDEFKRAKAIVEECKEELRKENIA
EEEEECHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
FDENVQVGMMVEIPAAAVNADKFSKYADFFSIGTNDLIQYSMAADRMSENVSYLYQPLNP
CCCCCCEEEEEECCHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCH
AILKLIDLTIKGAHKNNKVGMCGEMAGDIQALPLLLGMGLDAFSMSATSMLRARALMSKI
HHHHHHHHHEECCCCCCCCCCCHHHHCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
TMAEAEELANKALSSDDTPEVIELVDAFLATK
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7703858 [H]